Ercüment O. Sarihan

Efficient in vitro bulblet regeneration from immature embryos of endangered Sternbergia fischeriana

Sternbergia fischeriana is an endangered geophyte and therefore in vitro micropropagation of this plant will have great importance for germplasm conservation and commercial production. Bulb scale and immature embryo explants of S. fischeriana were cultured on different nutrient media supplemented with various concentrations of plant growth regulators. Immature embryos produced higher number of bulblets than bulb scales. Large numbers of bulblets were regenerated (over 80 bulblets/explants) from immature embryos on Murashige and Skoog (MS) medium supplemented with 4 mg l−1 6-benzylaminopurine (BA) and 0.25 mg l−1α-naphthaleneacetic (NAA) or 2 mg l−12,4-dichlorophenoxyacetic acid (2,4-D) after 14 months of culture initiation. Regenerated bulblets were kept at 5 °C for 5 weeks and then transplanted to a potting mixture.

Adventitious Shoot Regeneration and Micropropagation in Calendula officinalis L.

Hypocotyl, cotyledon and cotyledonary node explants of Calendula officinalis L were cultured on Murashige and Skoog (MS) media supplemented with various concentrations of thidiazuron (TDZ), kinetin (KIN), α-naphthaleneacetic acid (NAA) and indole-3-butyric acid (IBA) to induce adventitious shoot regeneration and micropropagation. The highest frequency of adventitious shoot regeneration was achieved from hypocotyl and cotyledon explants on MS media supplemented with 0.75 mg dm−3 TDZ and either 0.25 or 0.50 mg dm−3 IBA. Efficient in vitro clonal propagation was also induced from cotyledonary nodes on a range of media supplemented with 0.75 mg dm−3 TDZ and 0.05 mg dm−3 NAA or 2 mg dm−3 KIN and 1 mg dm−3 NAA. Regenerated shoots were excised and rooted in MS medium supplemented with 1 mg dm−3 NAA. The rooted plantlets were finally transferred to pots.