Erika Rosa Maria Hackmann

Determination of Piroxicam in Pharmaceutical Preparations By ultraviolet direct spectrophotometry, ultraviolet difference spectrophotometry and high performance Liquid Chromatography

Abstract Piroxicam in pharmaceutical preparations (capsules (C), tablets (T), oral drops (OD), suppositories (S) and simulated sample (SS)) was determined by UV direct spectrophotometry (UVS) at 333 nm, by UV difference spectrophotometry (UVDS) at 327 nm, and in C and T, by high performance liquid chromatography (HPLC). For UVS, Beers law was obeyed in the range 3.0 – 8.5 μg/mL. The coefficient of correlation (CC), absolute precision (AP) and relative precision (RP) were 0.9999, 0.02 and 0.33%, respectively. The coefficient of variation (CV) for C, T, OD, S and SS were 0.48%, 0.35%, 0.32%, 0.48% and 0.19%, respectively. The recovery average (RA) was 100.22%. For UVDS, Beers law was obeyed in the range 5.0 – 15.0 μg/mL. The CC, AP and RP were respectively 0.9999, 0.05 and 0.47%. The CV for C, T, OD, S and SS were 0.64%, 0.84%, 0.62%, 0.54% and 0.15%, respectively. The RA was 99.02%. In HPLC determination, a LiChrospher® 100 RP- 18 (5 μm) in LiChroCART® 125-4 column at ambient temperature with a mobile ph...