Erkan Yula

Investigation of neutrophil lymphocyte ratio and blood glucose regulation in patients with type 2 diabetes mellitus

Objective Leukocytosis is thought to be directly associated with the pathogenesis of atherosclerosis and metabolic syndrome. Increased white blood cell (WBC) count is related to cardiovascular disease in patients with type 2 diabetes mellitus; raised neutrophil lymphocyte ratio (NLR) is associated with metabolic syndrome. There is little information, however, concerning a correlation between glycosylated haemoglobin (HbA1c) and NLR. The aim of the present study was to investigate the relationship between NLR and blood glucose regulation. Methods This retrospective study was conducted in patients with type 2 diabetes mellitus, divided into two groups according to HbA1c levels: group 1, HbA1c levels ≤ 7%; group 2, HbA1c levels > 7%. Venous WBC, neutrophil and lymphocyte counts were determined. Results Of 71 patients included, fasting serum glucose, neutrophil and WBC counts were significantly higher in group 2 compared with group 1. NLR had a positive correlation with HbA1c. Conclusion There may be a significant relationship between NLR and blood glucose regulation. The authors propose that increased NLR may be associated with elevated HbA1c in patients with type 2 diabetes mellitus.

The effect of blood glucose regulation on the presence of opportunistic Demodex folliculorum mites in patients with type 2 diabetes mellitus.

Objectives To measure the rate of Demodex folliculorum mite infestation in patients with type 2 diabetes mellitus and to investigate if it was related to blood glucose control. Methods Patients with type 2 diabetes were classified according to their glycosylated haemoglobin (HbA1c) level into two groups: a well controlled blood glucose group (HbA1c ≤ 7%) and a poorly controlled blood glucose group (HbA1c > 7%). A standardized skin surface biopsy method was used to determine if the patients had D. folliculorum infestation (>5 mites/cm2 of skin). Results A total of 69 patients (38 female) were enrolled in the study. Seventeen (24.6%) patients had D. folliculorum infestation. There were no significant differences in age, sex or body mass index between patients with and without D. folliculorum infestations. A significantly higher proportion of patients with poor blood glucose control had D. folliculorum infestation compared with patients with well controlled blood glucose. Conclusions These current findings suggest that poor blood glucose regulation increases the susceptibility to D. folliculorum mite infestation in patients with type 2 diabetes.

Antifungal activity of several root canal sealers against Candida albicans

Abstract Objective. The purpose of this in vitro study was to evaluate the antifungal activity of several root canal sealers (iRoot SP, MTA Fillapex and GuttaFlow) against Candida albicans and compare them to that of AH Plus Jet. Materials and methods. A 10 μL fungi in suspension was allowed to directly contact the sealers, which were freshly mixed or allowed to set for 1 or 7 days. Fresh media was then added and survival of fungi was determined by using 10-fold serial dilution and inoculated onto agar plates. After incubation for 48 h, colony-forming units (CFU) were calculated and their log10 values converted. The data were analyzed using ANOVA and Tukey tests (α = 0.05). Results. Freshly mixed AH Plus Jet totally inhibited the growth of fungi and showed the highest antifungal activity. GuttaFlow did not show any significant antifungal activity at all times. Freshly mixed iRoot SP and MTA Fillapex were found to be antifungal. Statistical differences were found between freshly mixed and set samples (p < 0.05) in favor of the former, except GuttaFlow. No statistically significant differences were found among the tested sealers at the 1 and 7-day samples (p > 0.05). Conclusions. Fresh AH Plus Jet had very potent antifungal activity. All sealers, except GuttaFlow, exhibited antifungal activity when freshly mixed.

Bir hastanede gaita örneklerinde direkt mikroskopik inceleme ve ELISA ile Entamoeba histolitika araştırılması

Objectives: Stool antigen assay has been shown to be as sensitive and specific as culture with isoenzyme analysis and to outperform microscopy for the detection of E.histolytica in endemic area. The aim of the present study is to investigate the presence of E.histolytica by direct microscopic examination and ELISA in stool samples, comparatively. Materials and methods: Between September 2010 and May 2011, a total of 975 stool samples of patients in different age groups were sent to microbiology laboratory

Determination of antibiotic resistance and high-performance liquid chromatography profiles for Mycobacterium species

Incidence of mycobacterial infections has been increasing. However, diagnosis and treatment of mycobacterial infections can be difficult. The aim of this study was to investigate high‐performance liquid chromatography (HPLC) analysis of the mycolic acids for rapid identification and dendrogram cluster analysis of mycobacterium species.

To create awareness of serological tests instead of ERCP for fascioliasis diagnosis among physicians: A case report

Fascioliasis is a liver and biliary tract disease, which is rare in human. It is transmitted by consuming water plants containing Fasciola hepatica metacercariae or drinking contaminated water. Detection of parasite eggs in stool and serological/radiological tests are significant in fascioliasis diagnosis. A female patient who is 75-years-old applied to our clinic with one month duration of abdominal pain, nausea, vomit and anorexia. Routine biochemistry parameters of the patients with cholecystectomy were normal except her 8% eosinophilia. Endoscopic ultrasonography was planned in order to determine abdominal pain etiology and if there was a stone in ductus choledochus. Endoscopic retrograde cholangiopancreatography (ERCP) was applied for diagnosis and treatment purpose after the view of parasitecompatible view in ductus choledochus of the patient. Two parasites compatible with F. hepatica were taken out of ductus choledochus by ERCP. It was observed that the complaints of the patient, who was postoperatively treated with two doses of 10 mg/kg triclabendazole, was completely passed in control examination after two weeks. We present an acute cholangitis case depends on fascioliasis that we interventional diagnosed and treated, and the review of literatures on this case. OZET Fascioliasis, insanda nadir rastlanan karaciger

The Seropositivity of Parvovirus B19 in Patients with Ankylosing Spondylitis

Objective: Parvovirus B19 is one of the most common causes of viral arthritis, which suggests that it can be a significant factor in the pathogenesis of chronic inflammatory diseases, such as rheumatoid arthritis. The aim of the present study was to investigate the seropositivity of parvovirus B19 in patients with ankylosing spondylitis (AS). Material and Methods: The patient group consisted of 43 patients with ankylosing spondylitis; the control group consisted of 70 subjects who did not have any bone joints complaints. Anti-parvovirus B19 IgM and IgG antibodies were measured by ELISA. Results: Parvovirus B19 IgM antibody positivity in patients with AS was significantly higher than that in the control group (p<0.001). Conclusion: The high frequency of anti-parvovirus B19 IgM positivity in patients with AS suggests that the virus reactivates or persists in these patients due to immuno deficiency.