Euclides Lara Cardozo Junior
Universidade Paranaense
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Publication
Featured researches published by Euclides Lara Cardozo Junior.
Journal of Ethnopharmacology | 2014
Nayara Mercedes Frediani Brant; Francielly Mourão Gasparotto; Valdinei de Oliveira Araújo; Jhonatan Christian Maraschin; Rita de Cássia Lima Ribeiro; Emerson Luiz Botelho Lourenço; Euclides Lara Cardozo Junior; Arquimedes Gasparotto Junior
ETHNOPHARMACOLOGICAL RELEVANCE Although Casearia sylvestris Swartz is used in Brazilian folk medicine to treat obesity, no study has been conducted to evaluate the effects of this species in an experimental model of dyslipidemia and atherosclerosis. So, the aim of this study was to evaluate possible hypolipemiant and antiatherogenic activity of the methanolic extract obtained from Casearia sylvestris (MECS) using Swiss and C57BL/6 LDLr-null mice undergoing high fat diet (HFD). MATERIAL AND METHODS Dyslipidemia and atherogenesis were induced by the administration of commercial HFD for 4 weeks. The MECS was administered orally at doses of 250 and 500mg/kg, once a day, for two weeks, starting from the 2nd week of HFD. The gain in body weight and systolic blood pressure (SBP) were measured weekly over the four week study. At the end of the experiments the levels of triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) and very low-density lipoprotein cholesterol (VLDL-C) were measured by colorimetric method. Aldosterone, vasopressin and angiotensin converting enzyme (ACE) activity were also evaluated in collected serum. The renal function, atherogenic index serum (AIS) and in vitro antiplatelet activity were investigated. Additionally, histopathological analyzes were performed to determine the intima-media thickness (IMT) and intima media ratio (IMR) in aorta samples. RESULTS The HFD induced dyslipidemia and major structural changes in the aortic wall, including raising of the systolic blood pressure in LDLr-null mice. In addition, we observed an increase in lipid peroxidation accompanied by a reduction of serum nitrite. The treatment with MECS was able to prevent the increase of SBP, TC, LDL-C, VLDL-C and triglycerides levels and increase HDL-C in Swiss and LDLr-null mice. These effects were accompanied by a significant reduction in oxidative stress. Moreover, AIS, IMT and IMR were significantly reduced in MECS-treated mice, and the extract was able to reduce platelet aggregation in vitro. CONCLUSION This study demonstrated that MECS reduces the serum lipids and oxidative stress when orally administered to Swiss and LDLr-null mice. In addition, it was able to prevent arterial thickening induced by HFD and to inhibit platelet aggregation in vitro.
Pesquisa Agropecuaria Brasileira | 2010
Euclides Lara Cardozo Junior; Carmen Maria Donaduzzi; Osvaldo Ferrarese-Filho; Juliana Cristhina Friedrich; Adriana Gonela; José Alfredo Sturion
The objective of this work was to determine the contents of methylxanthines, caffeine and theobromine, and phenolic compounds, chlorogenic and caffeic acids, in 51 mate progenies (half-sib families) and estimate the heritability of genetic parameters. Mate progenies were from five Brazilian municipalities: Pinhao, Ivai, Barao de Cotegipe, Quedas do Iguacu, and Cascavel. The progenies were grown in the Ivai locality. The contents of the compounds were obtained by high performance liquid chromatography (HPLC). The estimation of genetic parameters by the restricted maximum likelihood (REML) and the prediction of genotypic values via best linear unbiased prediction (BLUP) were obtained by the Selegen - REML/BLUP software. Caffeine (0.248-1.663%) and theobromine (0.106-0.807%) contents were significantly different (p 0.5). The two different progeny groups determined for chlorogenic (1.365-2.281%) and caffeic (0.027-0.037%) acid contents were not significantly different (p<0.05) depending on the locality of origin. Individual heritability values were low to medium for chlorogenic (ĥ2<0.4) and caffeic acid (ĥ2<0.3). The content of the compounds and the values of genetic parameters could support breeding programs for mate.
Evidence-based Complementary and Alternative Medicine | 2014
Thiago Bruno Lima Prando; Tatiane da Fonseca Baciquete; Jennifer Alexandra Castanho Vieira; Jaqueline Bressan; Francielly Mourão Gasparotto; Douglas Rossi Jesus; Euclides Lara Cardozo Junior; Emerson Luiz Botelho Lourenço; Arquimedes Gasparotto Junior
Several species of the genus Piper are known in Brazilian folk medicine as having diuretic activity. So, we propose to investigate the acute diuretic activity and the possible toxic effects of Piper glabratum Kunth, popularly known as false Jaborandi. Additionally, we propose to check whether there is any correlation between the biological activities of the crude extract (MEPG) and its 2-methoxy-4,5-methylenedioxy-trans-cinnamoyl-pyrrolidine (MMCP) in Wistar rats. The MEPG was fractioned by chromatography column and the MMCP was identified by analyses of 1H and 13C RMN spectral data and correlations. Both MEPG and MMCP were assayed for diuretic activity. The preparations obtained were orally administered in a single dose to rats. The urine excretion, pH, density, conductivity, and content of Na+, K+, Cl−, and HCO3 − were measured in the urine of saline-loaded animals. Additionally, acute toxicity of the extract was also evaluated. MMCP at doses of 30 mg/kg was able to increase the urine volume, pH, and HCO3 − excretion. Moreover, high dosage of MEPG showed important liver toxicity and elevated mortality when injected intraperitoneally. The results indicate that the MMCP shows important diuretic properties when administered in Wistar rats. Additionally, MEPG can induce important acute toxicity if given in high doses.
Chemistry & Biodiversity | 2017
Juliana Cristhina Friedrich; Adriana Gonela; Maria Celeste Gonçalves Vidigal; Pedro Soares Vidigal Filho; José Alfredo Sturion; Euclides Lara Cardozo Junior
The aim of this study was to evaluate the phytochemical and genetic diversity, relationships and identification of mate (Ilex paraguariensis A.St.‐Hil.) elite genetic resources belonging to the Brazilian germplasm collection and mate breeding program. Mate has been studied due to the presence of phytochemical compounds, especially methylxanthines and phenolic compounds. The samples were collected from the leaves of 76 mate elite genetic resources (16 progenies × 5 localities). Total DNA was extracted from mate leaves and 20 random primers were used for DNA amplification. Methylxanthines (caffeine and theobromine) and phenolic compounds (chlorogenic, neochlorogenic, and criptochlorogenic acids) were quantified by HPLC. The genetic divergence estimated was higher within (92%) than among (8%) the different populations. Analysis of genetic distance between origins provided the formation of two groups by UPGMA cluster analysis, with higher polymorphism (94.9%). The average content of caffeine ranged from 0.01 to 1.38% and theobromine of 0.10 – 0.85% (w/w). The caffeoylquinic acids concentrations (1.43 – 5.38%) showed a gradient 3‐CQA > 5‐CQA > 4‐CQA. The coefficient of genetic variation (CVg) was of low magnitude for all mono‐caffeoylquinics acids. Significant correlations (positive and negative) were observed between the phytochemical compounds. Genetic diversity analysis performed by RAPD markers showed a greater intra‐populational diversity; genetic resources with low caffeine and higher theobromine content were identified and can be used in breeding programs; the correlation between methylxanthines and phenolic compounds can be used as a good predictor in future studies.
Evidence-based Complementary and Alternative Medicine | 2015
Valdinei de Oliveira Araújo; Francielly Mourão Gasparotto; Vanessa Aranega Pires; Aline Antunes Maciel; Caroline Flach Ortmann; Euclides Lara Cardozo Junior; Emerson Luiz Botelho Lourenço; Arquimedes Gasparotto Junior
Although Vitex megapotamica (Spreng.) Moldenke is used in Brazilian folk medicine as hypolipidemic drug no study has been conducted to evaluate the effects of this species in an experimental model of atherosclerosis. So, the aim of this study was to evaluate the possible renoprotective activity of methanolic extract obtained from Vitex megapotamica (MEVM) using C57BL/6 LDLr-null mice submitted to high fat diet (HFD). MEVM was orally administered at doses of 30, 100, and 300 mg/kg, for three weeks, starting from the 2nd week of HFD. Systolic blood pressure (SBP) and diuretic activity were measured weekly. At the end of experiments the serum lipids, atherogenic index serum (AIS), oxidative stress, and markers of renal function were determined. HFD induced a significant increase in the systolic blood pressure, dyslipidemia, increase in AIS, and lipid peroxidation accompanied by an important reduction in renal function. Treatment with MEVM was able to prevent increase in SBP, total cholesterol, triglycerides, AIS, urea, and creatinine levels in LDLr-null mice. These effects were accompanied by a significant reduction in oxidative stress and renal injury. The data reported here support the potential of Vitex megapotamica as candidate to be an herbal medicine used in cardiovascular or renal diseases.
Journal of Ethnopharmacology | 2015
Mônica Bolson; Sonia Regina Hefler; Elisiane Inês Dall’Oglio Chaves; Arquimedes Gasparotto Junior; Euclides Lara Cardozo Junior
Journal of Functional Foods | 2016
Euclides Lara Cardozo Junior; Christine Morand
Arquivos de Ciências da Saúde da UNIPAR | 2003
Carmen Maria Donaduzzi; Euclides Lara Cardozo Junior; Elenize Maria Donaduzzi; Miriam Machado da Silva; José Alfredo Sturion; Gabriel Correa
Revista Brasileira De Farmacognosia-brazilian Journal of Pharmacognosy | 2017
Cleide Adriane Slgnor Tirloni; Giseli Karenina Traesel; Francislaine Aparecida dos Reis Lívero; Salvador Dias Vieira Neto; Ronaldo de Faria Junior; Thaís C. Paim; Joyce Alencar Santos; Silvia Aparecida Oesterreich; Ariany Carvalho dos Santos; Roosevelt Isaias Carvalho Souza; Euclides Lara Cardozo Junior; Arquimedes Gasparotto Junior
Phytomedicine | 2017
Patrícia Gonçalves Santiago; Francielly Mourão Gasparotto; Karimi Sater Gebara; Flavia Barbieri Bacha; Francislaine Aparecida dos Reis Lívero; Maria Angélica Strapazon; Euclides Lara Cardozo Junior; Cândida Aparecida Leite Kassuya; Lauro Mera de Souza; Arquimedes Gasparotto Junior
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Elisiane Inês Dall’Oglio Chaves
Pontifícia Universidade Católica do Paraná
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