Ewelina Klichowska

Multivariate morphometric analysis of the Stipa turkestanica group (Poaceae: Stipa sect. Stipa)

Based on numerical analyses of macromorphological characters (cluster analysis, principal coordinate analysis and principal component analysis), scanning electron microscopy observation of lemma and lamina micromorphology, as well as field observations, five taxa belonging to the Stipa turkestanica group have been recognized in the mountain area of Central Asia. They are S. turkestanica subsp. turkestanica, S. turkestanica subsp. trichoides, S. macroglossa subsp. macroglossa, S. macroglossa subsp. kazachstanica and S. kirghisorum. As a result of this study, we propose one new combination, S. macroglossa var. pubescens, and designate lectotypes for S. turkestanica subsp. trichoides and S. macroglossa var. pubescens, and an epitype for S. kirghisorum. Illustrations of micromorphological structures of the lemma, patterns of leaf hairiness and an identification key are provided. A taxonomic synopsis including information on nomenclatural types, synonyms, descriptions of the taxa, and, as supplementary information, a list of the specimens examined is also presented.

Stipa pennata subsp. ceynowae (Poaceae, Pooideae), a new taxon from Central Europe

Abstract Based on numerical analyses of macromorphological characters, scanning electron microscopy observation of leaves and lemma micromorphology, as well as field observations, Stipa pennata subsp. ceynowae was described here as a new taxon from Poland. It differs from the most similar S. pennata subsp. pennata and S. borysthenica mainly by its longer ligules of vegetative shoots. The affinities of this taxon are discussed and a morphological comparison with related species is provided. Illustrations and images of the micromorphological structures, as well as information about its distribution, habitat and conservation status are given.

Plastid super-barcodes as a tool for species discrimination in feather grasses (Poaceae: Stipa)

Present study was designed to verify which or if any of plastome loci is a hotspot region for mutations and hence might be useful for molecular species identification in feather grasses. 21 newly sequenced complete plastid genomes representing 19 taxa from the genus of Stipa were analyzed in search of the most variable and the most discriminative loci within Stipa. The results showed that the problem with selecting a good barcode locus for feather grasses lies in the very low level of genetic diversity within its plastome. None of the single chloroplast loci is polymorphic enough to play a role of a barcode or a phylogenetic marker for Stipa. The biggest number of taxa was successfully identified by the analysis of 600 bp long DNA fragment comprising a part of rbcL gene, the complete rbcL-rpl23 spacer and a part of rpl23 gene. The effectiveness of multi-locus barcode composed of six best-performing loci for Stipa (ndhH, rpl23, ndhF-rpl32, rpl32-ccsA, psbK-psbI and petA-psbJ) didn’t reach 70% of analyzed taxa. The analysis of complete plastome sequences as a super-barcode for Stipa although much more effective, still didn’t allow for discrimination of all the analyzed taxa of feather grasses.

Development and characterization of microsatellite markers for endangered species Stipa pennata (Poaceae) and their usefulness in intraspecific delimitation

Stipa pennata (Poaceae), has become a rare and endangered species in Central Europe due habitat loss and fragmentation. This species is characterized by high morphological variability, which has resulted in the description of numerous intraspecific taxa. The aim of present work is to develop microsatellite markers useful in population genetics studies as well as in intraspecific taxonomy of S. pennata s.l. We developed ten microsatellite markers using Illumina high-throughput. Polymorphism at each marker was evaluated using 4–15 individuals from four morphotypes of S. pennata s.l. Seven markers showed polymorphism while three were monomorphic. The number of alleles per locus ranged from 7 to 12, and the observed and expected heterozygosity varied from 0.000 to 1.000 and 0.000 to 0.8670, respectively. Our results confirm that three of four studied morphotypes are genetically distinct. The microsatellite markers developed here will be useful for evaluating levels of genetic diversity and differentiation, to study gene flow, population dynamics and in future conservation studies as well as for intraspecific delimitation of morphologically similar taxa within S. pennata s.l.

A new record of Arabis recta Vill. (Brassicaceae) from Poland

Abstract Arabis recta Vill. is a therophyte currently considered to be endangered in Poland (EN category). The species has been reported from ca. 20 localities situated mainly in the Nida Basin (southeastern Poland). Arabis recta is usually a component of xero-thermophile grasslands, but it can also grow in arable fields or fallows. An abundant population of A. recta was found on slopes of the Góra Sosnówka hill (Małopolska Upland) in 2017. This newly discovered locality is currently the northernmost in Poland. The distribution of A. recta in Poland as well as collected phytosociological documentation are presented.

A new record of Typha shuttleworthii (Typhaceae) in Poland

Abstract The paper presents a new locality of Typha shuttleworthii W.D.J. Koch & Sond. in Poland. The species was found in wet roadside ditch in Kryg village near Gorlice (ATPOL grid square EG09). The distribution map of the species in Poland is provided.