Fabrício de Vargas Arigony Braga

Transplante autólogo de células mononucleares da medula óssea em úlcera de córnea experimental em cães

Bone marrow (BM) mononuclear cells (MC) are a great subject in tissual regeneration. The main stem cell source to the eye is the limbus. Theses cells replace injured corneal cells, however, if the limbal stem cells are not functional, a limbal deficiency with concomitant conjunctivalization takes place. This pathological condition can be caused for several reasons, in which alkali burns are the most common. To conduct a research about transplanted BM MC presence, the cells homing and to histopathologically compare the treated and sham group, an experimental corneal ulcer model associated with MC autotransplant was used. Sixteen, male or female, stray dogs suffered experimental corneal ulceration with sodium hydroxide soaked filter discs. After the lesions, animals were submitted to subconjunctival autotransplant of previously marked BM MC. The evaluation was made by immunofluorescence on the sixth day after lesions creation and histopathology was conducted 15 days after the same procedure, when it was possible to observe that the MC grafted in the injured area, the cells did not execute the homing process and, despite the inflammatory decrease, they did not help the corneal epithelial healing process in a short term evaluation. Thus, future studies about MC transplantations in corneal ulcers are indicated.

Hernioplastia diafragmática em cão com pericárdio bovino conservado em solução supersaturada de açúcar

Seven adult mongrel dogs, three males and four females, weighting between 10 and 22kg were used to evaluate the diaphragmatic muscle healing process. A defect of 8.0×5.5cm was created at the muscular portion of the left hemidiaphragm, through a thoracotomy at the eighth left intercostal space. A segment of bovine pericardium, preserved in 300% sugar solution, was implanted in the diaphragmatic defect. The heterologous implant was sutured with 3.0 polyamide using simple continuous suture. After the observation period, radiologic analysis and macroscopic and histopathologic evaluations were performed. Upon radiographic examination, there was no evidence of interruption of diaphragmatic outline or increased radiopacity at the implantation site. Histologically, fine connective tissue occluded the diaphragmatic defect. Bovine pericardium preserved in 300% supersaturated sugar solution at room temperature is replaced by a fine layer of connective fibrous tissue and promote repair of large diaphragmatic defects in dogs.

MÚSCULO DIAFRAGMA HOMÓLOGO CONSERVADO EM SOLUÇÃO SUPERSATURADA DE AÇÚCAR PARA REPARAÇÃO DE GRANDE DEFEITO NO DIAFRAGMA DE CÃO

The repair induced by an homologous muscular segment conserved in 300% supersaturated sugar solution on the diaphragmatic muscle of dogs was investigated. Nine adults mongrel dogs, three males, weights varying from 10.4 to 21.0kg, were used separated in three equally numbered denominated groups I, II and III. A defect was created, on the muscular portion of the right hemidiaphragm, with dimensions of 9.0 x 6.0cm, through thoracotomy in the 10th right intercostal space. The graft was sutured with thread poligalactin 910 3-0, by means of horizontal mattress suture, in an isolated pattern. The dogs were observed for a period of 30 days (subgroups I), 75 days (groups II) and 120 days (groups III) postoperatively. After this period, macroscopic observation and collection of samples for histologic evaluation were performed. It was verified in the animals of the subgroup I, partial substitution and in the subgroups II and III, total substitution of the muscular portion of the diaphragm. A fibrovascular tissue occluding the diaphragmatic defect was observed. With the postoperative evolution, a thin tissue, almost transparent, was identified. The segment of diaphragmatic muscle conserved in 300% supersaturated sugar solution, at room temperature, for repair of great defects in the diaphragmatic muscle of dogs was substituted by a fine layer of fibrous connective tissue.

Homoimplante de costela conservada em solução supersaturada de açúcar a 300% ou em açúcar in natura na reconstituição experimental de costelas em gatos

This study was aimed at evaluating the use of a homologous bone implant preserved sugar in natura (Ga) (8) or in 300% sugar solution (Gb) (8) for reconstitution of the thoracic wall in 16 cats, involving three ribs. A costal defect was made on the right side of the thoracic wall and the preserved ribs were used as homologous bone implants for the correction of this defect. These ribs were fixed to the receptor by means of a catheter needle. The animals were clinically evaluated by X-rays and histology. The percentual of implant incorporation at 60 days was 91.7% in the Ga and 73.9% in the Gb. A mild resorption was observed at different times, 29.2% in the in natura sugar group, and 37.8% in the 300% sugar group. No case of infection or rejection was observed in the evaluated period. The homologous rib bone implant preserved in sugar in natura or in 300% solution is efficient for the reconstitution of costal wall in cats.

Reparação da traquéia de cã o com segmaneto muscular homólogo de diafragma conservado em glicerina a 98

The viability of a homologous diaphragmatic muscle preserved in 98% glycerin for cervical tracheal repair in dogs was studied. Eighteen adult mongrel dogs, eight males, weighting 6 to 21kg were used. Five tracheal rings (2.0 x 4.5cm) were partially resected and a homologous muscle fragment was implanted. The animals were distributed in three numerally equal groups. They were observed for a post-operative period of 15 days (group I), 30 days (group II) and 60 days (group III). After these periods, the dogs were reoperated for macroscopic evaluation and collection of samples for histological evaluations. Tracheal repair with progressive substitution of the muscle flap by fibrous connective tissue, epithelial migration through the tracheal lumen and presence of cilia were detected. The homologous diaphragmatic muscle fragment preserved in 98% glycerin acts as a scaffold for the growth of fibrous connective tissue and allows epithelialization across the implant, without exuberant proliferation. Thus it can be used for the repair of tracheal defects in dogs.

Artrografia com meio de contraste positivo e duplo contraste na articulação do úmero de cães

The double contrast artrography is a diagnostic method in which positive contrast material (MCP) is injected in the joint space followed by negative contrast in order to outline lesions on the articular surfaces and adjacent structures. This technique is an effective and minimally invasive procedure already consolidated in human medicine as well as in the equine species with better results than the ones achieved by using usual diagnostic methods. In dogs this is not widely applied. The present study aimed to describe and evaluate the double contrast artrography technique applied to the humeral joint of canine species to compare with artrography with MCP in the evaluation of the articular surface of humerus head, intra-articular portion of the biceps muscle tendon, and articular capsule of eleven adult dogs. The double contrast within the joint has been produced by combining a small amount of iodine-based half of positive contrast covering the articular surfaces and capsular limits and a larger amount of a negative contrast filling the intra-articular space. The information collected from four radiologists have allowed to conclude that in the evaluation of the articular surface of the humerus head the double contrast artrography technique determinates a better image than with MCP. In addition, in the evaluation of the outline of the intra-articular portion of the biceps muscle tendon the artrography with MCP produced a better image than with double contrast artrography.

Membranas de látex natural na herniorrafia diafragmática experimental em cães

Latex membranes were experimentally used to repair diaphragmatic defects in 12 dogs, distributed in 3 groups. In group 1, a commercial membrane was used, and in group 2, an experimental membrane. Group 3, animals were used as control. A rectangular defect (4cm in length and 3cm in width) was surgically performed in the diaphragm muscle, which was substituted, in group 1 and 2, by the implantation of corresponding latex membrane. The animals were evaluated by radiography, blood count, video-surgery, and histologic study. Results showed that the latex membrane of group 2, were efficient in the correction of the defect in the diaphragm, promoting the repairing and local neovascularization, without causing rejection during the evaluated period.

Osso bovino esponjoso inorgânico liofilizado em bloco no reparo de fístula oronasal induzida em cães

The oronasal fistula repair still represents a challenge to the surgeons, due to the high incidence of recidivation. An experiment using 10 adult mongrel dogs weighting between 8 and 17 kilograms was performed in order to evaluate the in block lyophilized inorganic bovine bone cancellous (BLIBBC) in the treatment of this disease. Each dog had an exodontic procedure of both canine teeth, in order to produce an oronasal fistula. The surgical procedure realized consisted in two different surgical steps: the left side defect was repaired right after the defect have been produced, while the right side defect was repaired seven days after the fistula induction; both sides were repaired using BLIBBC and covered with gengival flap. The animals were radiological and clinicaly evaluated every week for 120 days. The material used was effective in the repair of both fistulas, without rejection or recurrence.

Pós-operatório de homoimplante de costela avaliado por toracoscopia paraxifóide transdiafragmática modificada em gatos

O objetivo deste estudo foi de avaliar a exequibilidade do acesso paraxifoide transdiafragmatico modificado, em gatos, no periodo pos-operatorio de homoimplante de costela para reconstituicao de parede toracica. Para tanto foram avaliados 16 gatos apos a reconstituicao de parede toracica com o uso de homoimplante de costela conservado em acucar. A cavidade toracica dos animais foi acessada por via transdiafragmatica modificada, por meio de uma incisao de 2cm na linha mediana paraxifoide para posterior introducao da cânula ao diafragma utilizando uma optica de 10mm de diâmetro. Foi identificado o terco medio da parede toracica, sendo necessario que os animais fossem posicionados em decubito lateral obliquado. Foram constatadas aderencias e visibilizado o aspecto dos implantes. Foi possivel avaliar a parede toracica de gatos com optica de 10mm de diâmetro atraves do acesso transdiafragmatico modificado.