Faez Firdaus Jesse Abdullah

Clinico-pathology, hematology and biochemistry responses in buffaloes towards Pasteurella multocida type B: 2 immunogen lypopolysaccharide via oral and intravenous routes of infection

Haemorrhagic septicaemia is a disease caused by Pasteurella multocida serotype B: 2 and E: 2. The organism causes acute, highly fatal septicaemic disease with high morbidity and mortality in cattle and more susceptible in buffaloes. Lipopolysaccharide can be found on the outer cell wall of the organism. Lipopolysaccharide is released during multiplication which leads to inflammatory reaction. It represents the endotoxin of P. multocida type B: 2 and responsible for toxicity in haemorrhagic septicaemia which plays an important role in the pathogenesis of the disease. Therefore, the aim of this study was to investigate the clinical signs, blood parameters, gross post mortem lesions and histopathology changes caused by P. multocida type B:2 immunogen lipopolysaccharide infections initiated through intravenous and oral routes of infection. 9 buffalo heifers were divided equally into 3 treatment groups. Group 1 was inoculated orally with 10 ml of phosphate buffer saline (PBS); Group 2 and 3 were inoculated with 10 ml of lipopolysaccharide broth intravenously and orally respectively. For the clinical signs, there were significant differences (p < 0.05) in temperature between the control, intravenous and oral group. In hematology and biochemistry findings, there were significant differences (p < 0.05) in erythrocytes, haemoglobin, PCV, MCV, lymphocytes, monocytes, eosinophils, GGT and albumin between the control, intravenous and oral group. However, there were no significant differences (p > 0.05) in the MCHC, leukocytes, band neutrophils, basophils, thrombocytes, plasma protein, icterus index, total protein, globulin and A:G ratio between intravenous and oral group. For Group 2 buffaloes, there were gross lesions in the lung, trachea, heart, liver, spleen, and kidney. In contrast, lesions were only observed in the lung, trachea and liver of Group 3 buffaloes. There were significant differences (p < 0.05) in hemorrhage and congestion; necrosis and degeneration; and inflammatory cells infiltration between experimental groups and control group. However, there were no significant differences (p > 0.05) in edema lesion between groups. In conclusion, this study is a proof that oral route infection of P. multocida type B:2 immunogen lipopolysaccharide can be used to stimulate host cell responses where oral vaccine through feed could be developed in the near future.

Clinico-pathology, hematology, and biochemistry responses toward Pasteurella multocida Type B: 2 via oral and subcutaneous route of infections

Background: Pasteurella multocida a Gram-negative bacterium has been identified as the causative agent of many economically important diseases in a wide range of hosts. Hemorrhagic septicemia is a disease caused by P. multocida serotype B:2 and E:2. The organism causes acute, a highly fatal septicemic disease with high morbidity and mortality in cattle and more susceptible in buffaloes. Therefore, the aim of this study was to investigate the clinical signs, blood parameters, post mortem and histopathology changes caused by P. multocida Type B:2 infections initiated through the oral and subcutaneous routes. Methods: Nine buffalo heifers were divided equally into 3 treatment groups. Group 1 was inoculated orally with 10 ml of phosphate buffer saline; Groups 2 and 3 were inoculated with 10 ml of 1012 colony forming unit of P. multocida Type B:2 subcutaneously and orally respectively. Results: There was a significant difference (p<0.05) in temperature between the subcutaneous and the control group. The results revealed significant differences (p<0.05) in erythrocytes, hemoglobin, packed cell volume, leukocytes, monocytes, and A: G ratio between the subcutaneous and the control group. Furthermore, there were significant differences (p<0.05) in leukocytes, band neutrophils, segmented neutrophils, lymphocytes, eosinophils, basophils, thrombocytes, plasma protein, icterus index, gamma glutamyl tranferase and A: G ratio between the oral and the control group. The post mortem lesions of the subcutaneous group buffaloes showed generalized hyperemia, congestion and hemorrhage of the immune organs, gastro-intestinal tract organs and vital organs. The oral group buffaloes showed mild lesions in the lung and liver. Histologically, there were significant differences (p<0.05) in hemorrhage and congestion; necrosis and degeneration; inflammatory cells infiltration; and edema in between the groups. Conclusion: This study was a proof that oral route infection of P. multocida Type B:2 can be used to stimulate host cell responses where oral vaccine through feed can be developed in the near future.

Clinico-pathological Features in Mice Following Oral Exposure to Pasteurella Multocida B: 2

Haemorrhagic septicaemia (HS) is a major cause of losses to livestock production in many countries around the world. In Malaysia, more specifically, the disease yet remains a major constraint to the national industry. However, the pathogenesis of haemorrhagic septicaemia is another scenario in which the limitations still exists. Thus, the present paper provides more information on the pathogenicity and host response dynamics in a mouse model. Our study of experimental nature manipulates P. multocida serotype B:2, the bacterium responsible for the disease in Asia. In this study, sixteen mice (n=16) were divided into two groups (A & B) of 8 mice each group. Animals in group A were inoculated orally with 1.0 ml 10 9 cfu/ml of P. multocida type B while mice in group 2 were challenged orally with 1.0 ml of phosphate buffer saline (PBS). The animals were observed for clinical signs for 5 days. The mice showing severe signs and surviving mice after 5 days of post- inoculation were euthanized using cervical dislocation approach and the organs such as heart, lung, kidney, stomach, spleen, colon and small intestine were collected for microscopic examinations. The result indicated that mice inoculated with the Pasteurella multocida showed significant (p<0.05) severe clinical signs compared to control group.These clinical signs ranged from mild to severe in which most of individuals infected with Pasteurella multocida showed moderate to severe clinical signs of ruffled hair, laboured breathing, eye discharge and responsiveness with mean levels of 2.13±0.64, 1.88±0.99, 1.50±1.20 and 1.88±0.99 respectively in comparison to the control group. Moreover, mortality rate was recorded between 24 to 50 h post-inoculation in the group that challenged with Pasteurella multocida type B: 2. Microscopically, the extent of visceral tissue damages due to the infection was scored. The interested parameters included pulmonary oedema, presence of inflammatory cells, haemorrhage and necrosis. Of these parameters, animals in infected group showed significant (p<0.05) differences in all most all visceral organs. Lungs, liver and kidney were, in particular, the most predominantly affected tissues. Therefore, oral inoculation of P. multocida type B in mice showed significant clinical response and cellular changes.

In vitro larvicidal effects of ethanolic extract of Curcuma longa Linn. on Haemonchus larval stage

Aim: Gastrointestinal helminthosis is a global problem in small ruminant production. Most parasites have developed resistance to commonly available anthelminthic compounds, and there is currently an increasing need for new compounds with more efficacies. This study evaluated the in vitro effects of ethanolic extract of Curcuma longa (EECL) as a biological nematicide against third stage Haemonchus larvae (L3) isolated from sheep. Materials and Methods: Haemonchus L3 were cultured and harvested from the feces of naturally infected sheep. EECL was prepared and three concentrations; 50, 100, and 200 mg/mL were tested for their efficacies on Haemonchus L3. Levamisole at concentration 1.5 and 3 mg/mL were used as positive controls. Results: EECL showed anthelmintic activity in a dose-dependent manner with 78% worm mortality within 24 h of exposure at the highest dose rate of 200 mg/mL. There was a 100% worm mortality rate after 2 h of levamisole (3 mg/mL) admisntration. However, there was a comparable larvicidal effect between when levamisole (1.5 mg/mL) and EECL (200 mg) were administered. Conclusion: The study shows that EECL does exhibit good anthelmintic properties at 200 mg/mL which is comparable with levamisole at 1.5 mg/mL.

Seroprevalence of Bovine Viral Diarrhea Virus Infection and Associated Risk Factors in Cattle in Selangor, Malaysia

Nurhusien Yimer/ Mohamed Ariff Omar,Jesse Faez Firdaus bin Abdullah,Rosnina Hj. Yusoff,Siti Suri Arshad,Abd Wahid Haron/ / , Kazhal Sarsaif,

Stage II Keratoconjunctivitis in a Goat: A Case report

A two year old Australian Feral male goat weighing 30 kg was presented to the large animal unit of Universiti Putra Malaysia Veterinary Hospital with the clinical signs of conjunctivitis and corneal ulceration with purulent ocular discharge. A sterile swab was taken from the eyefor bacteriological culture and the culture yielded a mixed growth of Pseudomonas aeroginosa and Moraxella caprae.The conditionwas diagnosed as pink eye disease. The goat was administered 3ml of 20mg/kgoxytetracyclinesubconjunctival injection of 0.4ml, intravenous injection of 2.2mg/kg Flunixin meglumine (antipyretic) agent, topical application of terramycine eye ointment and infusion of 2L of 0.6% Nacl solution. The signs of conjunctivitis and corneal ulcersof the eye had substantially reduced five days post treatment. Quarantine of infected animals, good quality feeding and fly control wasrecommendedas preventive measures. Keywords: Pink eye, goat, Moraxella caprae, bacteriology

Hemogram responses in goats toward challenged with Corynebacterium pseudotuberculosis and its immunogen mycolic acids

Aim:: This study was conducted to analyze the changes in blood profile of goats inoculated with Corynebacterium pseudotuberculosis and its immunogen mycolic acid (MA) extract. Materials and Methods:: A total of 12 clinically healthy crossbred Boer female goats were divided into three groups; A, B and C (4 goats each per group). Group A was inoculated with 2 ml sterile phosphate buffered saline via intradermal route as the negative control group whilst Group B was inoculated with 2 ml of MA extract (1 g/ml) intradermally and Group C was then inoculated with 2 ml (1×109) colony forming unit of active C. pseudotuberculosis intradermally. Blood sample was collected aseptically from the jugular vein periodically for complete blood count (CBC) analysis throughout the experimental period (3 months). Result:: A significant decrease (p<0.05) was observed in red blood cells, hemoglobin (Hb), packed cell volume, mean corpuscular volume and mean corpuscular Hb concentration in Groups B and C as compared to the control while WBCs, neutrophil, lymphocyte and basophil showed a significant increase (p<0.05) as compared to the control. Conclusion:: The inoculation of C. pseudotuberculosis and MA resulted in a significant change in the CBC, thereby, indicating that MA has a role in caseous lymphadenitis pathogenesis.

Isolation and detection of Corynebacterium pseudotuberculosis in the reproductive organs and associated lymph nodes of non-pregnant does experimentally inoculated through intradermal route in chronic form.

Aim: Corynebacterium pseudotuberculosis is the etiological agent of caseous lymphadenitis that affects sheep and goats. This study was designed to determine the presence of the causative organism in the female reproductive organs and associated lymph nodes in non-pregnant does experimentally inoculated through intradermal route in the chronic form. Materials and Methods: 18 non-pregnant healthy Katjang does aged 2-year-old were divided randomly into two groups. The first and second group consists of nine non-pregnant does each and the two groups were subdivided into three subgroups. The first group was experimentally inoculated with 1 ml of 107cfu of live C. pseudotuberculosis through intradermal route, whereas the second group was inoculated with 1 ml phosphate buffer saline (pH 7) solution intradermally. The first group were further subdivided into three subgroups where, the first subgroup (B1) were kept for 30 days post-infection, second subgroup (B2) were kept for 60 days post-infection, and third subgroup (B3) were kept for 90 days. The second group was further subdivided into three subgroups (C1, C2, and C3) where they were kept for 39, 60, and 90 days post-infection, respectively. Results: From this study, there was successful isolation of C. pseudotuberculosis from the reproductive organs of the treatment group after 60 days post-infection. The subgroups (B1, C1, C2, and C3) did not show any presence of the causative organism in the reproductive organs. The second subgroup B2 and third subgroup B3 showed positive isolation of the causative organisms from the ovary, uterine horns, uterus, cervix, vagina, and inguinal lymph node of the experimental non-pregnant does. Conclusion: This study showed that chronic infection of C. pseudotuberculosis via intradermal route may cause effect toward the reproductive organs and may be able to influence the reproductive efficiency of the infected animals.

Haematological, Biochemical And Serum Electrolyte Changes In Non-Pregnant Boer Does Inoculated With Corynebacterium Pseudotuberculosis Via Various Routes

Caseous lymphadenitis is a chronic disease characterized by internal and external abscesses and is caused by Corynebacterium pseudotuberculosis.Thisstudy is designed to measure the hematological, biochemicaland serum electrolyte changes in experimental non-pregnant doesinoculated with Corynebacterium pseudotuberculosis via variousroutes. Little is known about the changes in these parameters through different routes of infection.A total of 20 healthy does (n=20) were divided into 4 groups (intradermal, intranasal, oral and control) of 5 goats each. The three groups were inoculated with 107cfu/1ml of live Corynebacterium pseudotuberculosis, while control is kept unexposed. Following infection, blood samples were collected from the jugular vein for hematological, biochemical and serum electrolyteanalysis. A significantdecrease was observed in RBC count (p<0.05) in the intradermal group, whilenochangesobserved in PCV, Hb, MCV and MCHC parameters. Significant increasein WBC were observed in intradermal, intranasal and oral groups (p<0.05). Slight increase (p<0.05) in monocyte count was observed in intranasal group. A significant reduction (p<0.05) in lymphocytic count was observed for the intranasally inoculated group (p<0.05) and a slight increase (p<0.05) in neutrophil was observed in intranasal and intradermal groups. Biochemically,creatinine and albumin levels increases inintranasal group(p<0.05) and GGT levels were elevated in all the three infected groups (p<0.05).However,there were no significant changes in AST, T.Protein and APT. Serumelectrolyte, revealed a decrease in Calcium (Ca+) concentration in intradermal group with concentration of 2.22mmol/L,intranasal 2.23mmol/L group (p<0.05), and no changes were observed in potassium (K+) and sodium (Na+). The study, therefore, observed increase in WBCs, neutrophils, monocytes, creatinine, GGT, albumin levels and decrease in RBCs, lymphocytes and calcium concentrations on different route of infections.

Cytomegalovirus Replication Steps and the Actions of Antiviral Drugs

Human cytomegalovirus (HCMV) is a beta herpesvirus that inflicts an active infection in the fetus and immunosuppressive patients. The virus encodes many proteins that work together with cellular factors to achieve virus replication. In addition to vaccines, antiviral drugs can be deployed to manipulate how the virus replicates and minimize its pathogenicity. The five antiviral drugs approved by the Food and Drug Administration (FDA) have shown adverse reactions and the antiviral drug resistance were reported. Hence, this warrants the need for urgent development of a novel antiviral drug. Detailed understanding of the virus replication steps and how cellular signals interact with these steps will be key for pharmacological developments of for anti HCMV drugs. This review summarized all the drugs that target the virus proteins and cell signals that mediate CMV replication.