Abdinasir Yusuf Osman

Clinico-pathological Features in Mice Following Oral Exposure to Pasteurella Multocida B: 2

Haemorrhagic septicaemia (HS) is a major cause of losses to livestock production in many countries around the world. In Malaysia, more specifically, the disease yet remains a major constraint to the national industry. However, the pathogenesis of haemorrhagic septicaemia is another scenario in which the limitations still exists. Thus, the present paper provides more information on the pathogenicity and host response dynamics in a mouse model. Our study of experimental nature manipulates P. multocida serotype B:2, the bacterium responsible for the disease in Asia. In this study, sixteen mice (n=16) were divided into two groups (A & B) of 8 mice each group. Animals in group A were inoculated orally with 1.0 ml 10 9 cfu/ml of P. multocida type B while mice in group 2 were challenged orally with 1.0 ml of phosphate buffer saline (PBS). The animals were observed for clinical signs for 5 days. The mice showing severe signs and surviving mice after 5 days of post- inoculation were euthanized using cervical dislocation approach and the organs such as heart, lung, kidney, stomach, spleen, colon and small intestine were collected for microscopic examinations. The result indicated that mice inoculated with the Pasteurella multocida showed significant (p<0.05) severe clinical signs compared to control group.These clinical signs ranged from mild to severe in which most of individuals infected with Pasteurella multocida showed moderate to severe clinical signs of ruffled hair, laboured breathing, eye discharge and responsiveness with mean levels of 2.13±0.64, 1.88±0.99, 1.50±1.20 and 1.88±0.99 respectively in comparison to the control group. Moreover, mortality rate was recorded between 24 to 50 h post-inoculation in the group that challenged with Pasteurella multocida type B: 2. Microscopically, the extent of visceral tissue damages due to the infection was scored. The interested parameters included pulmonary oedema, presence of inflammatory cells, haemorrhage and necrosis. Of these parameters, animals in infected group showed significant (p<0.05) differences in all most all visceral organs. Lungs, liver and kidney were, in particular, the most predominantly affected tissues. Therefore, oral inoculation of P. multocida type B in mice showed significant clinical response and cellular changes.

Stage II Keratoconjunctivitis in a Goat: A Case report

A two year old Australian Feral male goat weighing 30 kg was presented to the large animal unit of Universiti Putra Malaysia Veterinary Hospital with the clinical signs of conjunctivitis and corneal ulceration with purulent ocular discharge. A sterile swab was taken from the eyefor bacteriological culture and the culture yielded a mixed growth of Pseudomonas aeroginosa and Moraxella caprae.The conditionwas diagnosed as pink eye disease. The goat was administered 3ml of 20mg/kgoxytetracyclinesubconjunctival injection of 0.4ml, intravenous injection of 2.2mg/kg Flunixin meglumine (antipyretic) agent, topical application of terramycine eye ointment and infusion of 2L of 0.6% Nacl solution. The signs of conjunctivitis and corneal ulcersof the eye had substantially reduced five days post treatment. Quarantine of infected animals, good quality feeding and fly control wasrecommendedas preventive measures. Keywords: Pink eye, goat, Moraxella caprae, bacteriology

Parasitic Gastro-Enteritis (PGE) Concurrent with Eperythrozoonosis in a Goat: A Case Report

A 7 month-old, male Katjang cross goat weighing 35kg with body condition score of 3/5 was presented to Large Animal Ward, University Veterinary Hospital (UVH). The goat had pale mucous membrane and diarrhoea. The goat had moderate normocytic, normochromic anaemia with packed cell volume (PCV) of 0.14 (0.22-0.32) and low normal plasma protein, neutrophilia with left shift and monocytosis, including hyponatraemia, hypocalcaemia and hypoalbuminaemia with increased muscle enzyme (CK). Thin blood film stained with Geimsa showed evidence of blood parasites and mycoplasma sp. 0.1 % of RBC was infected and with high strongyle infestation (13900 epg) using modified McMaster technique. Blood transfusion was done as a result of decreased PCV value to 0.13 on the second day of hospitalization. Parasitic gastro-enteritis (PGE) concurrent with eperythrozoonosis infection was diagnosed in the goat. Keywords: Parasitic gastro-enteritis, eperythrozoonosis, anaemia, blood transfusion, PCV

Comparative experimental study of Brucella melitensis and its lipopolysaccharide in mouse model infected via subcutaneous route of exposure

Brucella melitensis is a major zoonotic pathogen in which lipopolysaccharide (LPS) is believed to play a major role in the diseases pathogenesis. To study the immunopathophysiological aspects, we established a mouse model experimentally infected with whole cell of B. melitensis and its lipopolysaccharide via subcutaneous route of exposure. Eighty four mice, BALB/c, both sexes with equal gender distribution and 6-8 weeks-old were randomly assigned into 3 groups. Group 1 (n = 36) were subcutaneoulsy inoculated with 0.4 mL 109 of B. melitensis while group 2 (n = 36) were subcutaneously challenged with 0.4 mL 109 of LPS. Group 3 (n = 12) was challenged subcuatneously with phosphate buffered saline and served as a control group. Animals were observed for clinical signs, haematological and histopathological analysis for a period of 24 days post-inoculation. Our results revealed that B. melitensis infected group demonstrated significant clinical signs and histopathological evidence than LPS infected group. However, both infected groups showed elevated levels of interleukins (IL-1β & IL6), antibody levels (IgM & IgG) as early as 3 days post-infection with predominance in LPS infected group. For hormone analysis, low levels of progesterone, estradiol and testosterone were observed in both B. melitensis and LPS challenged groups throughout the study period. Moreover, in B. melitensis infected groups, the organism was re-isolated from the organs and tissues of gastrointestinal, respiratory and reproductive systems; thereby confirming the possible transmission of the disease dynamics. Moreover, LPS stimulated significantly the innate and acquired immune system without significant systemic dysfunction suggesting the potentiality of the protective properties of this component as an alternative vaccine for brucellosis infection. This report is the first detailed investigation comparing the infection progression and host responses in relation to the immunopathophysiological aspects in mouse model after subcutaneous inoculation with B. melitensis and its lipopolysaccharide.

A clinical case of navel and joint ill in a calf - medical management

An intensively managed five days old female Friesian cross breed calf weighing approximately 30kg was presented to the University Veterinary Hospital, Universiti Putra Malaysia (UPM). The complaint was that the calf had swollen navel and joints and had not been able to stand up properly and frequently fell down. But the calf was alert and the appetite was normal at the time of presentation. On physical examination, body condition score was 2.5 out of 5 and and temperature was 39.3 °C (slight pyrexia), pulse and respiratory rates were within the normal range. The navel was swollen with dark necrotic tissues within the navel opening with presence of foul smelling odour. Both the carpal joints of the forelimbs and the hock joints of the hind limbs were swollen. Arthrocentesis and Jugular venipuncture were performed to collect samples for synovial fluid cytology, haematology and blood chemistry. Based on the history, physical examination and laboratory findings, the calf was diagnosed to have navel ill with associated joint ill. Treatment was performed by umbilical debridement and cleaning of necrotic tissues and debris with subsequent application of povidone of iodine. Flunixin meglumine, 1.1 mg/kg, 0.7 mL, was given intramuscularly, twice daily for 3 days as analgesic, anti-inflammatory and antipyretic agent. A combination of penicillin and dihydrostreptomycin (1.2mL) at a dosage of 1 mL/25 kg body weight was administered intramuscularly, once daily for 5 days. Remarkable progressive improvement was observed 3 days after commencement of treatment. The umbilical care by debridement of necrotic tissues and debris and topical antisepsis, coupled with systemic antibiotic therapy using combination of penicillin and dihydrostreptomycin was effective in the treatment of navel ill and/or joint ill.

Stage Two Milk Fever in a Dairy Cow: A Case Report

The biological cycles of milk production and reproduction determine dairy profitability thus making management decisions dynamic and time-dependent. Metabolic diseases pose significant risk on net earnings of a dairy enterprise. We report a case of clinical hypocalcaemia (milk fever). A 7-year-old Jersey cross cow weighing 500 kg was presented with left lateral recumbency with the neck curved towards the flank. The case history indicated that the animal had calved two months ago and was semi-intensively managed. There was no history of vaccination and deworming. The daily production of milk was about 13 liters.

Foot rot due to Corynebacterium pyogenes infection in a cow: A Case Report

Foot rot is a painful condition resulting in moderate to severe lameness in cattle but with early intervention and vigorous treatment, most cases will have a favourable outcome. A 4 year old Kedah-Kelantan cow weighing 250kg was presented to the large animal unit of the University Veterinary Hospital, Universiti Putra Malaysia, with the complain of lameness for 5 days. Physical examination findings were pyrexia, inappetence and lameness. Blood analysis revealed neutrophilia with left shift, while the biochemical analysis revealed an increase in aspartate aminotransferase, creatine kinase and globulin levels. Bacterial culture from the swab samples of the cows hoof revealed growth of Corynebacterium pyogenes. The hoof was disinfected with povidone iodine and Benacillin injection (25mg/kg), Flunixine meglumine (1.1 mg/kg) and multivitamin (10mg/kg) injections were administered intramuscularly. The hoof was washed with 5% copper sulphate solution once daily. The prognosis was good after a follow up visit one week later. Early intervention is paramount in addressing foot rot caused by bacterial organisms in cattle.

Trypanosomosis in a Sambar Deer

A five years old Sambar deer was presented to the University Veterinary Hospital of Universiti Putra Malaysia. The deer was physically diagnosed with clinical signs of depression, lethargy, teeth grinding, pallor of mucous membranes, the vital parameters showed pyrexia (40.90C), increase in pulse (197beats/min.) and respiratory rates (42 circles/min.). Laboratory examination of the Buffy coat by micro haematocrit centrifugation technique revealed the presence of motile flagellates. Buffy coat smear examination confirmed the presence of Trypanosome spp. The disease was diagnosed based on clinical signs and laboratory finding. Diminazene Aceturate at a dose rate of 3.5mg/kg bodyweight was administered intramuscularly, coupled with multivitamin and fluid therapy proves effective in treating the condition. This report shows the significance of wild ruminants in maintaining the transmission cycle of this parasite and timely treatment could resolve the disease condition.

Management of Grade I Vaginal Prolapse in a Friesian Cross: A case report

A 350kg Friesian cross was presented to the Veterinary Teaching Hospital, University Putra Malaysia with a complaint of a bulging vagina one month after paturition. On physical examination, there was evidence of a grade I vaginal prolapse with presence of a yellowish mucoid secretion from the vulva. All the vital parameters were normal. Blood workout showed leucocytosis accompanied by neutrophilia with left shift, lymphocytosis and monocytosis. Creatine kinase was elevated ten folds, while serum globulin was only slightly elevated. There was a slight decline in calcium level in the blood. The prolapse was corrected surgically by applying Buhners suture. Systemic analgesic, antibiotic, multivitamin and calcium infusion were administered to minimize pain, prevent bacterial complication and normalize the calcium level in the blood, respectively. The prognosis was good and the prolapse regressed completely after a week. Prompt and timely management of vaginal prolapse is necessary in order to avoid serious complications that may predispose to uterine infections, infertility and death due to septicemia. Keywords: Vaginal prolapse; Diary cow; Buhners suture; Calcium

Therapeutic Activity of Crude Ethanolic Extract of Artemisia herba alba Against Trypanosoma evansi in Rabbits

The present work was conducted to evaluate the antitrypanosomal efficacy of crude ethanolic extract (CEE) of the aerial parts of Artemisia herba alba against Trypanosoma evansi infection in an animal model. The results indicated low levels of parasitaemia in rabbits administered with crude ethanolic extract (CEE) compared to those from the negative control group. Similarly, there was also haematologically significant difference (p<0.05) where low mean levels of packed cell volume (PCV) was observed in Groups 1-4 respectively. In contrast, there was no statistically significant difference in almost all investigated parameters between positive control and treatment groups of animals. In conclusion, both CEE of A. herba-alba and Berenil® showed relatively a parasitaemia and normal haematological values in infected rabbits, thereby confirming their antiparasitic properties.