Fagner Luiz da Costa Freitas

Aspectos clínicos e patológicos em frangos de corte (Gallus gallus domesticus) infectados experimentalmente com oocistos esporulados de Eimeria acervulina Tyzzer, 1929

3 ABSTRACT:- FREITAS, F.L. DA C.; ALMEIDA, K. DE S.; NASCIMENTO, A.A. DO; TEBALDI, J.H.; MA- CHADO, R.Z.; MACHADO, C.R. (Clinical and pathological aspects in broilers (Gallus gallus domesticus) infected experimentally with Eimeria acervulina Tyzzer, 1929 sporulated oocysts). Aspectos clinicos e patolo- gicos em frangos de corte (Gallus gallus domesticus) infectados experimentalmente com oocistos esporulados de Eimeria acervulina Tyzzer, 1929. Revista Brasileira de Parasitologia Veterinaria, v. 17, n. 1, p.16-20, 2008. Programa de Pos-Graduacao em Medicina Veterinaria, Departamento de Patologia Animal, Universidade Estadual Paulista, Campus de Jaboticabal, Av. Prof. Paulo Donato Castellane, s/n, Zona Rural, Jaboticabal, SP 14884-900, Brazil. E-mail: fagner@fcav.unesp.br. In the present study, clinical signs and pathological changes were evaluated during 30 days in broilers, lineage Cobb, males, with ten days old, infected with Eimeria acervulina. Were used 192 animals distributed in 3 groups: group A inoculated with 1x10 6 sporulated oocysts; group B inoculated with 1x10 5 sporulated oocysts; group C inoculated with distilled water. The clinical signs observed were anorexia, diarrhea and apathy. The pathological macroscopic changes observed were: enteritis, hyperemia with subsequent intestinal congestion, high concentration of mucus exudates in the lumen of the small intestine, pale and dehydration muscle, gall bladder full of liquid and deposition of hepatic fat. The villous atrophy and high presence of inflammatory cells were the microscopic changes observed in the gut epithelium. In histopathologic analysis of liver observed inflammatory cells infiltrated and fat deposition. The results demonstrate that broilers experimentally infected with E. acervulina presented progressive intestinal lesions of variable intensity and that these abnormalities are the main cause of reduction of bird performance.

Enzootic instability for bovine anaplasmosis on family farms located in southwestern Paraná, Brazil

The objective of this study was to assess the occurence of animals seropositive for Anaplasma marginale in the municipality of Realeza, Paraná State, Brazil. Blood samples were collected from 344 cows on 18 small farms in the municipality of Realeza-PR. The animalsserum samples were forwarded to the Federal University of Fronteira do Sul, in order to investigate the occurrence of anti-A. marginale IgG antibodies by an enzyme-linked immunosorbent assay commercial kit. IgG antibodies to A. marginale were detected in cattle from 77.7% of the farms. To the best authors knowledge, this is the first report of occurrence of A. marginale in cattle in southwestern Paraná. The serological assay showed that 24.4% of the animals were seropositive, thus characterizing the location investigated as an area of enzootic instability for the disease. The family farms located in the municipality of Realeza-PR showed enzootic instability for bovine anaplasmosis. It is necessary to conduct disease monitoring programs in association with preventive measures in order to ensure the sanitary quality of the herds and to reduce economic losses for the farmers. In addition, it is essential to implement educational extension actions that allow farmers to acquire knowledge, attitudes and perceptions regarding the risk factors that contribute towards herd A. marginale-infection.

Experimental Infection with Sporulated Oocysts of Eimeria maxima (Apicomplexa: Eimeriidae) in Broiler.

Through this study we assessed the metabolic and pathological changes in broilers experimentally infected with oocysts of Eimeria maxima. To perform the experiment, we used 150 broiler strain cooB males, with ten days of age, were randomized according to weight and randomly assigned to two experimental groups: the control group was inoculated with 0.5 mL of distilled water; the infected group inoculated with 0.5 mL of solution containing 5 × 104 sporulated oocysts of Eimeria maxima. The live performance was evaluated on day 0 (day of inoculation), 5°, 10°, 15°, 25°, and 35° dpi, being slaughtered by cervical dislocation, fifteen birds/group. Although the sum in meat production was higher in the control group, the weight of the heart and gizzard of the experimental animals showed no significant difference, while the liver had difference on day 5°, 15°, and 35° dpi. The pathologic evaluation showed congested mucosa and presence of large amounts of mucus at 6 dpi. Therefore, it is concluded that the dose of 5 × 104  E. maxima inoculated in the experimental group was enough to cause harm to the animal organism.