Fahrettin Gucin

Synthesis and investigation of antimicrobial activity of some bisbenzimidazole-derived chelating agents.

The 1,2-bis(2-benzimidazyl)-1,2-ethanediol (1), 1,4-bis(2-benzimidazyl)-1,2,3,4-butanetetraol (2), 1,3-bis(2-benzimidazyl)-2-thiapropane (3), 1,3-bis(2-benzimidazyl)-2-thia-propane-dihydrochloride (4), 1,5-bis(2-benzimidazyl)-3-thiapentane (5), and 1,5-bis(2-benzimidazyl)-3-thiapentane dihydrochloride (6) chelating ligands are synthesised and characterised by using analytical data and modem spectroscopic methods such as FT-Raman, FT-IR, 1H- and 13C-NMR spectrometers. Their antimicrobial activities are reported by comparing the in vitro activities, with those of ofloxacin, ciprofloxacin, piperacillin, ampicillin and cefazolin antibacterial agents against fresh clinical isolates. Antifungal activities are reported on Candida albicans, Candida utilis, Cryptococcus neoformans fungi, and the results are referenced with amphotericin-B, fluconazole and flucytosine antifungal agents. It has been found that all the compounds have broad spectra activity and was either more active or equipotent to those compared antibiotic and antifungal agents.

Antimicrobial Activity of Some Lactarius Species

The extracts obtained from six Lactarius species [Lactarius deterrimus Grager, Lactarius sanguifluus (Paul.: Fr.) Fr., Lactarius semisanguifluus Heim et Leclair, Lactarius piperatus Scop. ex Fr., Lactarius deliciosus (L. ex Fr.) S.F. Gray and Lactarius salmonicolor Heim et Leclair] have been investigated for their antimicrobial activity. Growth inhibition using agar disk diffusion assays was determined against: Escherichia coli ATCC 11230, Micrococcus luteus ATCC 2971, Stapylococcus aureus ATCC 6538P, Salmonella thyphi ATCC 19430, Klebsiella pneumoniae UC57, Pseudomonas aeruginosa ATCC 27853, Corynebacterium xerosis CCM 2824, Bacillus cereus ATCC 7064, Bacillus megaterium DSM 32, Mycobacterium smegmatis CCM 2067, Candida albicans ATCC10231 and Saccharomyces cerevisiae ATCC 9763. As a result of this study, we have found that Lactarius species revealed antimicrobial activity against some Gram (+) and Gram (-) bacteria, but showed no antagonistic effect against yeasts used in this study.

Synthesis, Raman, FT-IR, NMR spectroscopic characterization, antimicrobial activity, cytotoxicity and DNA binding of new mixed aza-oxo-thia macrocyclic compounds

Series of new mixed aza-oxo-thia macrocyclic ligands 1,9(2,6)-ditriazina-2,8,10,16-tetraaza-3,7,11,15-tetraoxo-5,13-dithia-cyclohexadecaphan-1(4),9(4)-diphenyl (L(1)); 1,10(2,6)-ditri azina-2,9,11,18-tetraaza-3,8,12,17-tetraoxo-5,6,14,15-tetrathia-cyclooctadecaphan-1(4),10(4)-diphenyl (L(2)); 1,11(2,6)-ditriazina-2,10,12,20-tetraaza-3,9,13,19-tetraoxo-6,16-dithia-cyclocosa-phan-1(4),11(4)-diphenyl (L(3)); 1,12(2,6)-ditriazina-2,11,13,22-tetraaza-3,10,14,21-tetraoxo-6,7,17,18-tetrathia-cyclodocosaphan-1(4),12(4)-diphenyl (L(4)) were synthesised. The structural features of the compounds have been studied by elemental analyses, Mass, FT-Raman, FT-IR, (1)H and (13)C NMR spectroscopy. The antimicrobial activities of the ligands were evaluated using disk diffusion method in dimethyl sulfoxide (DMSO) as well as the minimal inhibitory concentration (MIC) dilution method, against several bacteria and yeast cultures. The obtained results from both methods were assessed in side-by-side comparison with commercial antibacterial and antifungal agents. In most cases, the compounds show strong antifungal activity in the comparison tests. Cytotoxic activities of the ligands against two different human cancer cell lines, stomach (23132/87) and lung (A549) were determined by MTT assay. DNA fragmentation assay tested cell lines were used to analyze the DNA ladder formation which is a characteristic of apoptotic cell death. The binding of the ligands with calf thymus DNA (CT-DNA) has also been investigated by absorption spectroscopy.

A study on Antimicrobial Activity of Umbilicaria Crustulosa (ach.) Frey

in this study, the significance of recreation utiIizing activities inside the forests in Eskisehir and the demand for recreational use has been put forward comprehensively. Primarily the data related with human behaviours and recreational utilizing was gathered and it was evaluated variously statisiicaly.