Falls B. Hershey

QUANTITATIVE HISTOCHEMISTRY OF HUMAN SKIN

of el)i(lerlflis has i)eeli llan(licappe(l 1)y its histological complexity. The micro methods devised by I)r. 0. H. Lowry have pernlitte(l accurate Samfling of various ((‘11 ty )es in brain, an(l other structures. In this a jer, (lata are l)resentecl testing :tIuI vali(lating nlinor nlodiflcations of these lilethO(15 that are useful for the studs’ of various (‘elI types in litlIllali skin. Study of the enzynles of skin :tiicl especially of (‘j)i(lerIllis has 1)een Ilalldicapped also by iifficulty in se )arating and honiogenizing elji(lerl1lis, an(I at’t’urate sanl )ling of the at’tive layers an(l structures. At’curate sanl )hng is necessary 1)ecaUSe the sul)jacent (lernus is relatively inactive . Soine of tllese difficulties have l)een circulllveflte(l so that sn all 5itIlll)les of particular tell tVl)es (all 1)e analvze(l . ‘lilt’ methods are sufficiently l)reeiSe and setisitive to l)(’rlllit conll)arison of nlicrogr:ull 5aI1 l)les. ‘i’his )a )er rel)orts nleasurenlent of five

Operation for aneurysm of the internal carotid artery high in the neck: a new and an old technique.

From the Department of Surgery, Washington University School of Medicine, and the Vascular/Surgical Service, St. John’s Mercy Hospital, St. Louis, Missouri. * Chief of Vascular Surgery, St. Johns Mercy Medical Center, St. Louis, Missouri. Aneurysms of the internal carotid artery high in the neck challcnge the judgment and ingenuity of the vascular surgeon. The ideal treatment of aneurysms is resection and restoration of arterial continuity. However, cases are encountered in which the distal artery may be difficulty to control by clamps and impossible to anastomose.3 ~ 4 , 8,9,10 Therefore the vascular surgeon should be familiar with all the techniques of reconstruction as well as methods of ligation. This paper reports a new internal shunt which prevented back bleeding from the Circle of Willis, and which conducted flow to the brain during repair of an internal carotid aneurysm which extended up to the base of the skull. The aneurysm was repaired and a normal channel reconstructed by the technique invented by Matas in 1903 and used for popliteal aneurysms in the early years, the &dquo;endoaneurysmorrhaphy&dquo;. Carotid aneurysms are a rare lesion, comprising less than 10 % of all peripheral aneurysms, and the internal carotid is less commonly involved than the common carotid or external.

PHOSPHOPYRIDINE NUCLEOTIDE LINKED DEHYDROGENASES, ALDOLASE AND FUMARASE IN MOUSE BREAST

I . Methods are Presented for nieasuring five enzymes in 1 to 5 y frozen-dried samples of mouse mammiuar duct, fat or conmiective tissue. 2. Effects of incubation temperature, pH, and substrate and pyridine nucleotide concentrations (Ofl activity of the five mammary enzymes are reported. 3. Lactic, mahic and isocitric dehiydrogenases are stable for at least 49 days in mouse mamniary gland wheni stored frozen and dcied at -20#{176}C. Aldolase amid fumarase activities dechine after 21 days. 4. Activities of these five enzymes amid of glucose-6-phosphate dehuydrogenase do not significantly change from estrus to diestrus in mouse

Quadrilateral Peripheral Vascular Disease in the Young Adult

Seven cases of quadrilateral chronic occlusive arterial disease different from other known chronic occlusive arterial diseases as evidenced by arteriography, pathology, and clinical manifestations are reported. They resemble some of the cases originally described by Buerger.

HORMONAL EFFECTS ON GLUCOSE-6-PHOSPHATE DEHYDROGENASE OF MOUSE MAMMARY GLAND

Phuosl)hate dehydrogenase activity in frozen and dried sections of mouse mammary gland duct. The methods (2, 7) have been modified to give optimum activity. Characteristics of the enzyme in homogenates are reported. Striking changes imi activity of the enzyme occur in various hormomual states of the niouse. lnhuihition of the enzyme by certain C17 and C20 ketosteroids is reported. Activity of this enzyme in breast hiomogenates has beemi previously reporte(1 to increase markedly durimig lactation (9). Activity of homogenates of breast cancer of the rat decreases after ovariectomv (10). Inhibitiomi in various other tissues of the rat amid humnan by pregnenobone and other C7 amid Czo ketosteroids is reported by Marks

Chromaffin-Reacting Cells of Digital Skin in Peripheral Vascular Diseases

* From the Departments of Pathology, Surgery, and Internal Medicine, Washington University School of Medicine, Barnes Hospital and U. S. Veterans Administration Hospital, St. Louis, Missouri. Supported by grants (CRT-5017) and (RG 4192 C3) from the National Institutes of Health, Public Health Service, Bethesda, Maryland. t United States Public Health Trainee in Experimental Pathology (Grant No. CRT-5017). ‡ We are grateful to Dr. H. N. K. Luke and Dr. R. A. Sutter for these samples. Chromaffin-positive granules have been found in human skin,1 . 4 and may play a role in controlling skin circualtion.4 A technique of demonstrating these chromaffin-reacting cells in punch biopsies of normal human digital skin