Franc Lobnik

Microbial Community Structure During Composting With and Without Mass Inocula

Inoculation of a mixture of household organics and shredded wood with inocula from the active phase of composting enhanced mineralization of organic matter and yielded a biologically stabilized product with a more favorable C/N ratio than in a non-inoculated treatment. Analysis of phospholipid fatty acids was used to determine total viable microbial biomass and relative amounts of bacteria, Gram positive and Gram negative bacteria, actinomycetes, fungi, thermophiles and nonthermophiles in compost. The initial viable microbial biomass was 6-fold higher in inoculated than in noninoculated compost. The thermophilic phase occurred immediately after inoculation and the total viable biomass afterwards followed the course of temperature. The ratio of viable (sum of phospholipid fatty acids) to dead (sum of diglyceride fatty acids) microbial biomass during this period was generally higher at lower composting temperatures in both inoculated and noninoculated compost. During composting, fluctuations in total viable microbial biomass and in the relative amounts of indicator PLFAs of all microbial groups except Gram negative bacteria was more intensive in noninoculated compost.

Single application of sewage sludge - impact on the quality of an alluvial agricultural soil.

The effects of sewage sludge on soil quality with regard to its nutrient and heavy metal content, microbial community structure and ability to maintain specific soil function (degradation of herbicide glyphosate) were investigated in a three months study using an alluvial soil (Eutric Fluvisol). Dehydrated sewage sludge significantly increased soil organic matter (up to 20.6% of initial content), total and available forms of N (up to 33% and 220% of initial amount, respectively), as well as total and plant available forms of P (up to 11% and 170% of initial amount, respectively) and K (up to 70% and 47% of initial amount, respectively) in the upper 2 cm soil layer. The increase of organic matter was most prominent 3d after the application of sewage sludge, after 3 months it was no longer significant. Contents of nutrients kept to be significantly higher in the sewage sludge treated soil till the end of experiment. Contents of some heavy metals (Zn, Cu, Pb) increased as well. The highest increase was found for Zn (up to 53% of initial amount), however it was strongly bound to soil particles and its total content was kept below the maximum permissible limit for agricultural soil. Based on molecular fingerprinting of bacterial 16S rRNA gene and fungal ITS fragment on 3rd day and 3rd month after sewage sludge amendment, significant short term effects on bacterial and fungal communities were shown due to the sewage sludge. The effects were more pronounced and more long-term for bacterial than fungal communities. The mineralization of (14)C-glyphosate in the sewage sludge soil was 55.6% higher than in the control which can be linked to (i) a higher glyphosate bioavailability in sewage sludge soil, which was triggered by the pre-sorption of phosphate originating from the sewage sludge and/or (ii) beneficial alterations of the sewage sludge to the physical-chemical characteristics of the soil.

Chemometric characterization of soil and plant pollution: Part 1: Multivariate data analysis and geostatistical determination of relationship and spatial structure of inorganic contaminants in soil

Geostatistical and multivariate methods of data analysis are used to describe patterns of soil pollution with inorganic contaminants in Celje County, Slovenia. Groups of contaminants and polluted sites were identified using cluster analysis and confirmed with multidimensional variance and discriminant analysis. Factor analysis yields an identification of not directly observable relationships between the contaminants. The spatial structure and distribution of contaminants were assessed by applying semivariogram analysis and kriging interpolation method. Zinc, Cd and Cu were identified as a pollutant emitted from the zinc smelter, Pb also from other sources, and Cr and Ni mostly from geological parent material.

Prediction of cadmium concentration in selected home-produced vegetables

Soil contaminated with cadmium presents a potential hazard for humans, animals and plants. The latter play a major role in the transfer of cadmium to the food chain. The uptake of cadmium and its accumulation by plants is dependent on various soil, plants and environmental factors. In order to identify soil properties with statistically significant influence on cadmium concentration in vegetables and to reduce the collection of data, time and costs, regression models can be applied. The main objective of this research was to develop regression models to predict the concentration of cadmium in 9-vegetable species: zucchini, tomato, cabbage, onion, potato, carrot, red beet, endive and chicory, based on soil properties. Soil samples were collected from 123 home gardens of the Municipality of Celje and 59 of these gardens were also included in vegetable sampling. The concentration of elements (e.g. arsenic, cadmium, copper, lead, and zinc) in the samples was determined by Inductively Coupled Plasma Mass Spectrometry. Single (for cabbage, potato, red beet and chicory) and multiple (for tomato, onion, carrot and endive) linear regression models were developed. There was no statistically significant regression model for zucchini. The most significant parameter for the influencing the cadmium concentration in vegetables was the concentration of cadmium in soil. Other important soil properties were the content of organic matter, pH-value and the concentration of manganese. It was concluded that consuming carrots, red beets, endives, onions, potatoes and chicory which are grown in gardens with Cd concentrations (mgkg(-1) DW) above 2.4, 3.2, 6.3, 7.9, 8.3 and 10.9, respectively, might represent an important contribution to dietary Cd exposure.

Statistical analyses of soil properties on a quaternary terrace sequence in the upper sava river valley, Slovenia, Yugoslavia

Abstract Alpine glaciations, climatic changes and tectonic movements have created a Quaternary sequence of gravely carbonate sediments in the upper Sava River Valley, Slovenia, Yugoslavia. The names for terraces, assigned in this model, Gunz, Mindel, Riss and Wurm in order of decreasing age, are used as morphostratigraphic terms. Soil chronosequence on the terraces was examined to evaluate which soil properties are time dependent and can be used to help constrain the ages of glaciofluvial sedimentation. Soil thickness, thickness of Bt horizons, amount and continuity of clay coatings and amount of Fe and Me concretions increase with soil age. The main source of variability consists of solutions of carbonate, leaching of basic cations and acidification of soils, which are time dependent and increase with the age of soils. The second source of variability is the content of organic matter, which is less time dependent, but varies more within soil profiles. Textural changes are significant, presented by solution of carbonate pebbles and sand, and formation is silt loam matrix, which with age becomes finer, with clay loam or clayey texture. The oldest, Gunz, terrace shows slight deviation from general progressive trends of changes of soil properties with time. The hypothesis of single versus multiple depositional periods of deposition was tested with one-way analysis of variance (ANOVA) on a staggered, nested hierarchical sampling design on a terrace of largest extent and greatest gravel volume, the Wurm terrace. The variability of soil properties is generally higher within subareas than between areas of the terrace, except for the soil thickness. Observed differences in soil thickness between the areas of the terrace could be due to multiple periods of gravel deposition, or to the initial differences of texture of the deposits.

Nitrogen isotope enrichment factor as an indicator of denitrification potential in top and subsoil in the Apace Valley, Slovenia

Denitrification is still a poorly explained process in soil nitrogen cycles. Nitrogen isotope analyses, in combination with conventional soil-science methods (i.e. rate measurements), permit the tracing and quantification of several turn-over and transfer processes in soils. The aim of the presented investigations is to determine the 15N isotope enrichment factor (e) in a laboratory experiment during denitrification in the topsoil and subsoil layer of a Eutric Fluvisol (FAO classification) from the Apace Valley (NE Slovenia). Intact soil cores taken from depths of 0.10–0.20 m and 0.90–1.00 m were incubated under anaerobic conditions at a temperature of 18°C. A nitrate dose of 116 mg N-NO3/L was added to the topsoil layer and 58 mg N-NO3/L to the subsoil material. Glucose (250 mg) was added to each soil column. After the initial 24 h of incubation, water samples were taken every 8 h. The isotope enrichment factor for denitrification in the upper soil-layer columns was –7.60 ± 1.28‰ and for subsoil columns –34.91 ± 1.77‰ (n = 3). The isotope enrichment factor close to zero demonstrated that microorganisms easily consumed abundant substrate in the topsoil layer. Added nitrate and glucose exceeded the capacity of the microbial population in the subsoil and the process was limited by a low microbial population for which a high e is characteristic.