Francesco Lucertini

Exercise as a new physiological stimulus for brown adipose tissue activity

BACKGROUND AND AIM Brown adipose tissue (BAT) plays a major role in body energy expenditure counteracting obesity and obesity-associated morbidities. BAT activity is sustained by the sympathetic nervous system (SNS). Since a massive activation of the SNS was described during physical activity, we investigated the effect of endurance running training on BAT of young rats to clarify the role of exercise training on the activity and recruitment state of brown cells. METHODS AND RESULTS Male, 10-week-old Sprague Dawley rats were trained on a motor treadmill (approximately 60% of VO2max), 5 days/week, both for 1 and 6 weeks. The effect of endurance training was valuated using morphological and molecular approaches. Running training affected on the morphology, sympathetic tone and vascularization of BAT, independently of the duration of the stimulus. Functionally, the weak increase in the thermogenesis (no difference in UCP-1), the increased expression of PGC-1α and the membrane localization of MCT-1 suggest a new function of BAT. Visceral fat increased the expression of the FOXC2, 48 h after last training session and some clusters of UCP-1 paucilocular and multilocular adipocytes appeared. CONCLUSION Exercise seemed a weakly effective stimulus for BAT thermogenesis, but surprisingly, without the supposed metabolically hypoactive effects. The observed browning of the visceral fat, by a supposed white-to-brown transdifferentiation phenomena suggested that exercise could be a new physiological stimulus to counteract obesity by an adrenergic-regulated brown recruitment of adipocytes.

The Pleiotropic Effect of Physical Exercise on Mitochondrial Dynamics in Aging Skeletal Muscle

Decline in human muscle mass and strength (sarcopenia) is one of the principal hallmarks of the aging process. Regular physical exercise and training programs are certain powerful stimuli to attenuate the physiological skeletal muscle alterations occurring during aging and contribute to promote health and well-being. Although the series of events that led to these muscle adaptations are poorly understood, the mechanisms that regulate these processes involve the “quality” of skeletal muscle mitochondria. Aerobic/endurance exercise helps to maintain and improve cardiovascular fitness and respiratory function, whereas strength/resistance-exercise programs increase muscle strength, power development, and function. Due to the different effect of both exercises in improving mitochondrial content and quality, in terms of biogenesis, dynamics, turnover, and genotype, combined physical activity programs should be individually prescribed to maximize the antiaging effects of exercise.

Muscle Stiffness and Rate of Torque Development during Sprint Cycling

PURPOSE Crank torque (CT) application and rate of CT development (RCTD) are important considerations in sprint cycling. The stiffness of the musculotendinous unit is related to the isometric rate of torque development (RTD); however, this relationship has yet to be examined in sprint cycling. METHODS Maximal isometric torque (MIT) and isometric RTD of the quadriceps were assessed in 21 trained male cyclists (28.7 +/- 9.5 yr, 1.74 +/- 0.08 m, and 67.5 +/- 7.2 kg). Unilateral musculoarticular (MA) stiffness of the quadriceps was quantified using an oscillation test. Further, the participants performed a maximal 6-s sprint to assess peak power output (POpeak), peak CT (CTpeak), peak RCTD (RCTDpeak), and the crank angles associated with CTpeak and RCTDpeak. Participants were ranked on MA stiffness properties and were divided into a relatively stiff group (SG) and a relatively compliant group (CG). RESULTS The SG displayed a significantly higher MA stiffness than the CG (P < 0.05). Furthermore, the SG reported significantly elevated MIT (27%), RTD (26%), and RCTDpeak (16%) when compared with the CG (P < 0.05), along with trends for increased POpeak (7%) and CTpeak (8%). The angles at CTpeak and RCTDpeak were 7% and 12% lower for the SG, respectively (P < 0.05). MA stiffness was significantly correlated with RCTDpeak, MIT, RTD, and POpeak. CONCLUSIONS Higher stiffness is related to superior RCTDpeak in trained cyclists during a single sprint. A significant proportion of the variance in RCTDpeak was attributed to MA stiffness (37%), which was of greater magnitude than the relationship between RCTDpeak and MIT. Furthermore, the lower CTpeak angle and RCTDpeak angle may contribute to a more rapid development of CT. Accordingly, MA stiffness seems to be an important consideration for sprint cycling.

A maximal isokinetic pedalling exercise for EMG normalization in cycling

An isometric maximal voluntary contraction (iMVC) is mostly used for the purpose of EMG normalization, a procedure described in the scientific literature in order to compare muscle activity among different muscles and subjects. However, the use of iMVC has certain limitations. The aims of the present study were therefore to propose a new method for the purpose of EMG amplitude normalization in cycling and assess its reliability. Twenty-three cyclists performed 10 trials of a maximal isokinetic protocol (MIP) on a cycle ergometer, then another four sub-maximal trials, whilst the EMG activity of four lower limbs muscles was registered. During the 10 trials power output (CV=2.19) and EMG activity (CV between 4.46 and 8.70) were quite steady. Furthermore, their maximal values were reached within the 4th trial. In sub-maximal protocol EMG activity exhibited an increase as a function of exercise intensity. MIP entails a maximal dynamic contraction of the muscles involved in the pedalling action and the normalization session is performed under the same biomechanical conditions as the following test session. Thus, it is highly cycling-specific. MIP has good logical validity and within-subject reproducibility. Three trials are enough for the purpose of EMG normalization in cycling.

Muscle Releases Alpha-Sarcoglycan Positive Extracellular Vesicles Carrying miRNAs in the Bloodstream

In the past few years, skeletal muscle has emerged as an important secretory organ producing soluble factors, called myokines, that exert either autocrine, paracrine or endocrine effects. Moreover, recent studies have shown that muscle releases microRNAs into the bloodstream in response to physical exercise. These microRNAs affect target cells, such as hormones and cytokines. The mechanisms underlying microRNA secretion are poorly characterized at present. Here, we investigated whether muscle tissue releases extracellular vesicles (EVs), which carry microRNAs in the bloodstream under physiological conditions such as physical exercise. Using density gradient separation of plasma from sedentary and physically fit young men we found EVs positive for TSG101 and alpha-sarcoglycan (SGCA), and enriched for miR-206. Cytometric analysis showed that the SGCA+ EVs account for 1–5% of the total and that 60–65% of these EVs were also positive for the exosomal marker CD81. Furthermore, the SGCA-immuno captured sub-population of EVs exhibited higher levels of the miR-206/miR16 ratio compared to total plasma EVs. Finally, a significant positive correlation was found between the aerobic fitness and muscle-specific miRNAs and EV miR-133b and -181a-5p were significantly up-regulated after acute exercise. Thus, our study proposes EVs as a novel means of muscle communication potentially involved in muscle remodeling and homeostasis.

Effects of Fatigue on Muscle Stiffness and Intermittent Sprinting during Cycling

PURPOSE It was recently demonstrated that musculoarticular (MA) stiffness is related to sprint cycling performance in nonfatigued conditions. This study examined whether relatively stiffer cyclists were more effective at sprinting under fatigued conditions, as occurs during endurance cycling competitions. METHODS MA stiffness of the quadriceps was assessed in 21 trained male cyclists (28.7 ± 9.5 yr, 1.74 ± 0.08 m, 67.5 ± 7.2 kg). Participants also performed a maximal 6-s sprint on a cycle ergometer to assess peak power output (POpeak), peak crank torque (CTpeak), and peak rate of crank torque development (RCTDpeak). A cycling fatigue protocol then required cyclists to pedal at 30%, 35%, and 40% of POpeak and sprint at the end of each stage. Surface EMG was recorded from vastus lateralis during each sprint and analyzed in the time domain as integrated EMG (iEMG) and in the frequency domain as instantaneous median frequency (MDF) adopting a continuous wavelet transform. Participants were then retested for MA stiffness. RESULTS MA stiffness (-12%) was significantly reduced after the cycling protocol. Further, POpeak, CTpeak, RCTDpeak, and iEMG were reduced by 20%, 15%, 13%, and 20%, respectively, after the fatigue protocol (P<0.05). When the cyclists were divided into relatively stiff (SG) and relatively compliant groups (CG), only SG exhibited significant decreases in MA stiffness, CTpeak, RCTDpeak (P<0.05), and instantaneous MDF (R=0.705). CONCLUSIONS Whereas neuromechanical parameters were generally reduced under conditions of fatigue, stiff and compliant cyclists were affected differently, with the sprint abilities of SG decreased to the level of CG. It seems important for endurance cyclists to incorporate training strategies to maintain MA stiffness during competition to offset declines in sprint performance.

Effectiveness of professionally-guided physical education on fitness outcomes of primary school children.

Abstract Physical education (PE) at school is an important starting point for long-term interventions improving quality of life in elderly. To evaluate the effectiveness of professionally led PE on motor and health-related abilities of Italian primary schoolchildren (3rd–5th graders), three schools were assigned to the experimental groups “A” (38 pupils, 17 M, 21 F) and “B” (37 pupils, 16 M, 21 F), and to control group “C” (26 pupils, 18 M, 8 F). All groups underwent a six-month, twice-a-week (60 min each session) PE intervention. The PE program of the EGs was age-tailored, included strength training and was administered by specialised teachers. Group A and B programs differed in the strength training devices used, while they were identical in terms of training load. The control group program was not structured and administered by generalist teachers. At baseline and follow-up, children underwent a motor and health-related abilities test battery. At follow-up, children in group C gained significantly more weight than children in the EGs and scored significantly less than the children in the EGs in the following assessments: counter movement jump (C:+0.15% vs. A:+4.1% and B:+6.99%), plate tapping (C:+13.56% vs. A:+19.37% and B:+36.12%), sit-and-reach (C:−311.15% vs. B:+409.57%), pinch strength (C:+2.39% vs. B:+10.83, on average) and sit-up (C:+29.69% vs. A:+72.61%). In conclusion, specialist-led pupils demonstrated greater increases in some motor and health-related abilities tests compared to generalist-led peers, while different strength training devices produced comparable increases of strength in both EGs.

Physiological and biomechanical responses to walking underwater on a non-motorised treadmill: effects of different exercise intensities and depths in middle-aged healthy women

Abstract Non-motorised underwater treadmills are commonly used in fitness activities. However, no studies have examined physiological and biomechanical responses of walking on non-motorised treadmills at different intensities and depths. Fifteen middle-aged healthy women underwent two underwater walking tests at two different depths, immersed either up to the xiphoid process (deep water) or the iliac crest (shallow water), at 100, 110, 120, 130 step-per-minute (spm). Oxygen consumption (VO2), heart rate (HR), blood lactate concentration, perceived exertion and step length were determined. Compared to deep water, walking in shallow water exhibited, at all intensities, significantly higher VO2 (+13.5%, on average) and HR (+8.1%, on average) responses. Water depth did not influence lactate concentration, whereas perceived exertion was higher in shallow compared to deep water, solely at 120 (+40%) and 130 (+39.4%) spm. Average step length was reduced as the intensity increased (from 100 to 130 spm), irrespective of water depth. Expressed as a percentage of maximum, average VO2 and HR were: 64–76% of peak VO2 and 71–90% of maximum HR, respectively at both water depths. Accordingly, this form of exercise can be included in the “vigorous” range of exercise intensity, at any of the step frequencies used in this study.

Trunk muscles activation during pole walking vs. walking performed at different speeds and grades.

Given their functional role and importance, the activity of several trunk muscles was assessed (via surface electromyography-EMG) during Walking (W) and Pole Walking (PW) in 21 healthy adults. EMG data was collected from the external oblique (EO), the erector spinae longissimus (ES), the multifidus (MU), and the rectus abdominis (RA) while performing W and PW on a motorized treadmill at different speeds (60, 80, and 100% of the highest speed at which the participants still walked naturally; PTS60, PTS80 and PTS100, respectively) and grades (0 and 7%; GRADE0 and GRADE7, respectively). Stride length, EMG area under the curve (AUC), muscles activity duration (ACT), and percentage of coactivation (CO-ACT) of ES, MU and RA, were calculated from the averaged stride for each of the tested combinations. Compared to W, PW significantly increased the stride length, EOAUC, RAAUC and the activation time of all the investigated muscles, to different extents depending on treadmill speeds and grades. In addition, MUAUC was higher in PW than in W at GRADE0 only (all speeds, p<0.01), while ESAUC during W and PW was similar at all the speeds and grades. These changes resulted in longer CO-ACT in PW than W, at GRADE0-PTS100 (p<0.01) and GRADE7 (all speeds, p<0.01). In conclusion, when compared to W, PW requires a greater engagement of the abdominal muscles and, in turn, a higher control of the trunk muscles. These two factors taken together may suggest an elevated spinal stability while walking with poles.

High Cardiorespiratory Fitness Is Negatively Associated with Daily Cortisol Output in Healthy Aging Men

Physical fitness has salutary psychological and physical effects in older adults by promoting neuroplasticity and adaptation to stress. In aging, however, the effects of fitness on the hypothalamic-pituitary-adrenal (HPA) axis are mixed. We investigated the association between cardiorespiratory fitness and HPA activity in healthy elderly men (n = 22, mean age 68 y; smokers, obese subjects, those taking drugs or reporting recent stressful events were excluded), by measuring in saliva: i) daily pattern of cortisol secretion (6 samples: 30’ post-awakening, and at 12.00, 15.00, 18.00, 21.00, 24.00 h); and ii) the cortisol response to a mental challenge. Cardiorespiratory fitness (VO2max) was estimated using the Rockport Walking Test and the participants were assigned to high-fit (HF, ≥60°, n = 10) and low-fit (LF, ≤35°, n = 12) groups according to age-specific percentiles of VO2max distribution in the general population. At all daytimes, basal cortisol levels were lower in the HF than the LF group, most notably in the evening and midnight samples, with a significant main effect of physical fitness for cortisol levels overall; the area-under-the-curve for total daily cortisol output was significantly smaller in the HF group. Among the subjects who responded to mental stress (baseline-to-peak increment >1.5 nmol/L; n = 13, 5 LF, 8 HF), the amplitude of cortisol response and the steepness of recovery decline displayed an increasing trend in the HF subjects, although between-group differences failed to reach the threshold for significance. In conclusion, cardiorespiratory fitness in healthy aging men is negatively correlated with daily cortisol output and contributes to buffering the HPA dysregulation that occurs with advancing age, thus possibly playing a beneficial role in contrasting age-related cognitive and physical decline.