Francisco Carlos de Lima

Monitoramento histopatológico de mexilhão Perna perna da Lagoa de Itaipu, Niterói, RJ

Histologic sections of 120 mussels Perna perna (Linnaeus, 1758), from Itaipu Lagoon, Niteroi, State of Rio de Janeiro, Brazil, were examined for the presence of parasites and lesions during a period of 12 months. Ninety mussels showed five different pathological processes: bucephalosis, protozoan Nematopsis sp., basophilic non-specific inclusions, parasitic encapsulations and ctenidia epithelial cysts. Fifty animals presented two or more pathological processes at the same time.

Infecção por Kudoa Meglitsch, 1947 (Myxozoa: Multivalvulida) em musculatura esquelética de espada Trichiurus lepturus L. (Teleostei: Trichiuridae)

The presence of Kudoa sp in somatic muscle of cutless fish (Trichiurus lepturus) captured at Itaipu beach, Niteroi, Rio de Janeiro, is been reported. It was used as samples 47 specimes, measured and necropsied at the Laboratorio de Inspecao e Tecnologia de Pescado da Faculdade de Veterinaria daUniversidade Federal Fluminense. The tissues were processed for paraffin inclusion and stained by hematoxilina-eosin. From the total samples, nine of them (19,15%) showed plasmodic type cists in muscular fibers without inflammatory reaction. It was four valves, the principal feature of the genus. The number of cysts varied from one to 15, with a average of 5,1. The length and width average of cysts were 82,57 and 28,27, respectively. The length average of positive fishes was 116cm. The smaller animal (68,0) showed more cysts. It was not observed myoliquefaction even several hours after captured

Aspectos gametogênicos e histoquímicos de Iphigenia brasiliana (Lamarck) (Bivalvia, Donacidae) da Lagoa de Itaipu, Niterói, Rio de Janeiro, Brasil

Gametogenic and histochemical aspects of an edible bivalve, lphigenia brasiliana (Lamarek, 1818) from Itaipu Lagoon, Niteroi, State of Rio de Janeiro, were studied. Between May 1998 and October 1999 a total of 120 clams were picked up. Samples of 10 animals were collected once a month. Specimens were fixed in Bouins fixative for 4 hours or less and transferred to 10% formalin for 24 hours. The shell lenght has been measured with the valves lightly opened to permit the penetration of fixative solution deeply in the tissues. Transverse sections including digestive diverticula, mantle, gills, gonad and foot were obtained from the total body. After fixative treatment, tissues sections were embedded in paraffin, cut routinely at 5 µm and stained with Harriss hematoxylin and eosin. Microscopic analysis was carried out at low and high dry magnifications, as well as under oil immersion. Advanced ova stages were measured. Special staining techniques have been applied as a tentative tool for searching glycogen, mucous cells, collagenic and reticulin fibers, RNA and protozoan organisms. The animals collected showed variation in lenght from 7.20 cm to 4.10 cm and in height from 4.60 cm to 3.10 cm. The sex ratio (M:F) was 40:69. The average ova diameter of I. brasiliana was 34.70 µm with a standard deviation of 5.19 µm. Nothing could be observed in I. brasiliana, concerning to lesions and parasites at histopathological analysis.

Ciclo reprodutivo do mexilhão Perna perna (Linnaeus) (Molusca, Bivalvia) da Lagoa de Itaipu, Niterói, Rio de Janeiro, Brasil

The gametogenic aspects of mussel Perna perna (Linnaeus, 1758) from Itaipu Lagoon, Niteroi, were studied through November 1998 to October 1999. Microscopic analysis was carried out at low and high dry magnifications, as well as under oil immersion. The animals collected showed variation of size from 4.8 to 9.1 cm in total length and from 2.0 to 3.9 cm in total width. Most advanced gonadal stage was detected through May, August and October of 1999 for female specimens. Male advanced gonadal stage occurred in September of the same year. The sex-ratio (M:F) for P. perna was 64:52.

Epitheliocystis disease in Tilapia nilotica (Linnaeus, 1758) from Rio de Janeiro State, Brazil

Branchial cysts are described in eight specimens of Tilapia nilotica from the state of Rio de Janeiro, Brazil. Cysts consisted of hypertrophy of branchial epithelial cells, which dysplayed a single basophilic corpuscle. The disease reported has the microscopic hallmarks of epitheliocystis.

HISTOPATOLOGIA DO PARASITISMO POR METACERCARIAS DE Clinostomum sp EM TAMOATA, Hoplosternum littorale (HANCOCK, 1828)

Ahistopathological study of the parasitism by metacercaries of genera Clinostomum was caried out in 26 fishes of the species Hoplosternum littorale. The fishes were collected in Guama River - Belem City - Para State. 85% of them showed the parasitism. Microscopically, degenerated muscular fibers around of the cystics formations and moderated inflamatory response with mononuclear cells infliltration were observed, being diagnosed as parasitary miositis