František Kocourek

Spiders (Araneae) in the biological and integrated pest management of apple in the Czech Republic

Abstract:  Two management systems, biological and integrated, were compared to control the major pest, codling moth (Cydia pomonella) in apple orchards. The aim of the study was to assess the effect of these two systems on arboreal spiders. The biological system was based on the use of biological preparations, whereas in the integrated system selective pesticides were employed. The control plot had no pesticide treatment. The abundance of spiders was similar in all study plots during 3 years of study. Diversity, however, was higher in the biological plot than in the control and the integrated plots, suggesting that the response of spiders to management was guild‐specific. Four spider families dominanted in all plots: Araneidae (orb weavers), Theridiidae (space‐web weavers), and Philodromidae and Thomisidae (ambushers). While Araneidae and Thomisidae were similarly abundant on all plots, the density of Theridiidae and Philodromidae differed. In the integrated plot there were significantly more theridiid spiders, whereas in the control plot philodromid spiders were significantly more abundant. On the biological plot, the two families were similarly abundant. These differences were attributed to different age of trees in the control and treated plots, different prey spectrum, different susceptibility of the two families to applied chemicals and intraguild predation of theridiids by philodromids.

Effect of azadirachtin applied systemically through roots of plants on the mortality, development and fecundity of the cabbage aphid (Brevicoryne brassicae)

The effect of low concentrations of azadirachtin applied systemically through root tissues of rape plants (Brassica napus subsp.napus) on the mortality, period of development, longevity and fecundity of the cabbage aphid (Brevicoryne brassicae L.) (Sternorrhyncha: Aphididae) was studied. The concentrations of azadirachtin A tested were: 0.25, 0.05, 0.005, 0.0005 and 0.00005 mg ml−1. The mortality of cabbage aphid nymphs, particularly during the period of ecdysis, increased significantly with increasing concentrations. Azadirachtin had no effect on the length of the development period of the respective immature stages of cabbage aphids. The average longevity of the cabbage aphids decreased with increasing azadirachtin concentration and time spent feeding. The fecundity of aphids decreased after the application of azadirachtin.

Flight activity of winter rape pests.

The paper presents the results of a 5‐year research on Ceutorhynchus napi, C. pallidactylus, C. assimilis and Meligethes aeneus, pests of winter rape. The population dynamics, flight activity, changes in sex ratio, and oogenesis of these pests were studied using yellow water‐traps. The sums of effective temperatures for flight activity (SETF) were determined. The sex ratio of the above species showed distinct changes, the percentage of females being higher than that of males. Also, there was a considerable difference between the development of ova in the ovarioles of females captured in the water‐traps and those found on rape plants. The use of the SETF and yellow water‐traps in practical pest control is discussed.

Detection of organophosphate and pyrethroid resistance alleles in Czech Leptinotarsa decemlineata (Coleoptera: Chrysomelidae) populations by molecular methods.

BACKGROUND Pyrethroids and organophosphates are the most frequently used insecticides for Colorado potato beetle, Leptinotarsa decemlineata (Say), control in the Czech Republic. Based on molecular methods, organophosphate and pyrethroid resistance alleles have been detected in samples from three sites. The accuracy of restriction fragment length polymorphism (RFLP) and bidirectional polymerase chain reaction amplification of specific alleles (Bi-PASA) for detection of resistance alleles is compared. RESULTS Leptinotarsa decemlineata from three sites showed higher frequencies of resistance alleles to organophosphates than to pyrethroids. The rates of occurrence of individuals homozygous resistant (RR) to pyrethroids ranged from 20.0 to 22.9%, while the rates of occurrence of individuals RR to organophosphates ranged from 52.9 to 66.7%. The incidences of individuals with resistance alleles to both organophosphates and pyrethroids ranged from 8.6 to 13.6%. No relationship was found between incidence of the pyrethroid resistance allele and site, while incidence of the organophosphate resistance allele differed significantly according to site. CONCLUSION Both RFLP and Bi-PASA were suitable for detecting resistance alleles to pyrethroids, and in most cases also for detecting resistance alleles to organophosphates. In contrast to Bi-PASA, RFLP was also suitable for samples with lower DNA quality when testing for the resistance allele to pyrethroids. On the other hand, RFLP was not as accurate as Bi-PASA in detection of the organophosphate resistance allele.

The effect of integrated pest management practices in an apple orchard on Heteroptera community structure and population dynamics.

Abstract: The community structure and population dynamics of Heteroptera was studied in an apple orchard in the Czech Republic, between 1992 and 1995. The study investigated the changes which occurred after introduction of integrated pest management (IPM) practices into an intensive apple orchard. The IPM consisted of establishing grass ground cover or planting six selected herb species in two wide belts along the rows of trees. The IPM areas were compared with areas where chemical control was continued. Seventy Heteroptera species were captured (22 predatory and 48 phytophagous). The diversity of heteropteran communities was always higher on IPM plots and six predatory species and 24 phytophagous species were only captured on the IPM plots. Annual variation of abundance of predatory species on IPM plots was smaller than in phytophagous species. Orius spp. were abundant on the chemical control areas due to abundant Tetranychus urticae C.L. Koch populations, which were probably a consequence of the application of pyrethroid insecticides. Other predatory species were more abundant in IPM areas due to higher prey availability. The abundance of phytophagous species was favoured by the herbaceous undergrowth of the IPM plots. The number of predatory Heteroptera species increased after the introduction of IPM practices. Vegetation cover diversity is enhanced due to cultivation of the plots with IPM regime and has not resulted in any additional increase in the abundance of predatory Heteroptera species.

Beyond the survival and death of the deltamethrin-threatened pollen beetle Meligethes aeneus: An in-depth proteomic study employing a transcriptome database

Insecticide resistance is an increasingly global problem that hampers pest control. We sought the mechanism responsible for survival following pyrethroid treatment and the factors connected to paralysis/death of the pollen beetle Meligethes aeneus through a proteome-level analysis using nanoLC coupled with Orbitrap Fusion™ Tribrid™ mass spectrometry. A tolerant field population of beetles was treated with deltamethrin, and the ensuing proteome changes were observed in the survivors (resistant), dead (paralyzed) and control-treated beetles. The protein database consisted of the translated transcriptome, and the resulting changes were manually annotated via BLASTP. We identified a number of high-abundance changes in which there were several dominant proteins, e.g., the electron carrier cytochrome b5, ribosomal proteins 60S RPL28, 40S RPS23 and RPS26, eIF4E-transporter, anoxia up-regulated protein, 2 isoforms of vitellogenin and pathogenesis-related protein 5. Deltamethrin detoxification was influenced by different cytochromes P450, which were likely boosted by increased cytochrome b5, but glutathione-S-transferase ε and UDP-glucuronosyltransferases also contributed. Moreover, we observed changes in proteins related to RNA interference, RNA binding and epigenetic modifications. The high changes in ribosomal proteins and associated factors suggest specific control of translation. Overall, we showed modulation of expression processes by epigenetic markers, alternative splicing and translation. Future functional studies will benefit. BIOLOGICAL SIGNIFICANCE Insects develop pesticide resistance, which has become one of the key issues in plant protection. This growing resistance increases the demand for pesticide applications and the development of new substances. Knowledge in the field regarding the resistance mechanism and its responses to pesticide treatment provides us the opportunity to propose a solution for this issue. Although the pollen beetle Meligethes aeneus was effectively controlled with pyrethroids for many years, there have been reports of increasing resistance. We show protein changes including production of isoforms in response to deltamethrin at the protein level. These results illustrate the insects survival state as a resistant beetle and in its paralyzed state (evaluated as dead) relative to resistant individuals.

Evaluation of pesticide residue dynamics in Chinese cabbage, head cabbage and cauliflower

ABSTRACT Pesticide residues from the time of application until harvest were analysed for 20, 17 and 18 active insecticidal and fungicidal substances in Chinese cabbage, head cabbage and cauliflower, respectively. In total, 40 mathematical models of residue degradation were developed using a first-order kinetic equation, and from these models it was possible to forecast the action pre-harvest interval for a given action threshold for low-residue production in Brassica vegetables as a percentage of the maximum residue level. Additionally, it was possible to establish an action pre-harvest interval based on an action threshold of 0.01 mg kg‒1 for the production of Brassica vegetables for baby food. Among the evaluated commodities, the speed of residue degradation was highest in head cabbage, medium in Chinese cabbage and lowest in cauliflower. The half-lives of pesticide in various vegetables were also determined: they ranged from 1.55 to 5.25 days in Chinese cabbage, from 0.47 to 6.54 days in head cabbage and from 1.88 to 7.22 days in cauliflower.

Seven‐year monitoring of pyrethroid resistance in the pollen beetle (Brassicogethes aeneus F.) during implementation of insect resistance management

BACKGROUND An increase in the spread of pyrethroid resistance in the pollen beetle has been documented in many European countries. Pyrethroid resistance in the pollen beetle in the Czech Republic has been detected using a topical application bioassay. Resistance monitoring has been carried out during the implementation of insect resistance management. RESULTS The susceptibilities of nine pollen beetle populations to four pyrethroids in 2009 - 2015 in the Czech Republic are presented in this paper. The highest resistance ratio (RR) values [based on the lethal dose for 50% of the population (LD50 )] obtained for deltamethrin, lambda-cyhalothrin, tau-fluvalinate and etofenprox were 500, 299, 108 and 66.9, respectively. Pollen beetle mortality after application of deltamethrin or lambda-cyhalothrin gradually decreased from 2009 to 2013. High cross-resistance between lambda-cyhalothrin and deltamethrin and low cross-resistance between lambda-cyhalothrin and etofenprox were demonstrated. A kdr mutation known to cause resistance in the pollen beetle was not detected. CONCLUSION A high level of resistance was recorded in most of the nine populations of the pollen beetle in 2013. In the following 2 years, a decrease in resistance associated with a population density decrease of the pollen beetle was observed in all nine populations as a result of insect resistance management based on pyrethroid reduction in winter oilseed rape.