Frédéric Cazaux
university of lille
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Frédéric Cazaux.
Acta Biomaterialia | 2011
Thomas Laurent; I. Kacem; N. Blanchemain; Frédéric Cazaux; Christel Neut; H.F. Hildebrand; Bernard Martel
The aim of this work was to develop a polypropylene (PP) artificial abdominal wall implant for the prolonged release of ciprofloxacin (CFX). This sustained release effect was obtained by functionalization of the textile mesh with citric acid and hydroxypropyl-γ-cyclodextrin (HPγCD) or maltodextrin (MD). In both cases the textile finishing reaction yielded a cyclo- or malto-dextrin crosslinked polymer coating the fibers. The modified supports were characterized by thermogravimetric analysis (TGA), differential scanning calorimetry and scanning electron microscopy. The sorption capacities and the kinetics of CFX release were studied by batch tests coupled with spectrophotometric assays. Microbiological assays were carried out on Staphylococcus aureus, Staphylococcus epidermidis and Escherichia coli, while proliferation and viability tests used fibroblasts. The main results were as follows. (i) Due to the differences between the range of temperature of thermal degradation of the (cyclo)dextrins polymers and of the PP fibers TGA was a reliable method for quantifying the degree of functionalization of the textiles. (ii) Both modified supports showed improved sorption/desorption capacities for CFX, compared with the virgin mesh. The HPγCD-finished support showed an increased sorption capacity and a lower release rate of CFX compared with the MD modified support. (iii) Microbiological assays confirmed the latter result, with greater sustained antibacterial activity of the HPγCD treated support. These experiments have demonstrated the role of the cyclodextrin cavity in interactions with CFX: the antibiotic was not only adsorbed via hydrogen and acid-base interactions with the polyCTR-HPγCD network, but also via host-guest complexation. (iv) Biological tests revealed a slight decrease in fibroblast proliferation after 6 days on the modified supports, but cell viability tests showed that this was not due to toxicity of the (cyclo)dextrin polymer coatings.
Carbohydrate Polymers | 2013
Adeline Martin; Nicolas Tabary; Laurent Leclercq; Jatupol Junthip; Stéphanie Degoutin; François Aubert-Viard; Frédéric Cazaux; Joël Lyskawa; Ludovic Janus; Marc Bria; Bernard Martel
The aim of this work was to develop the formation of multilayered coating incorporating a cyclodextrin polyelectrolyte onto a non-woven polyethylene terephthalate (PET) textile support in order to obtain reservoir and sustained release properties towards bioactive molecules. We optimized the multilayer assembly immobilization onto the PET surface according to the layer-by-layer (LbL) deposition process. After a pre-treatment of the textile support aiming to offer a sufficient ionic character to the surface, it was alternatively immersed into two polyelectrolytes aqueous solutions consisting of chitosan (CHT) as polycation on the one hand, and a β-cyclodextrin polymer (polyCTR-βCD) as polyanion on the other hand. In a second approach, a TBBA/polyCTR-βCD complex (4-tert-butylbenzoic acid, TBBA) was used in order to load the system with a drug model whose kinetics of release was assessed. Gravimetry, microscopy, OWLS, colorimetric titration, infrared and zetametry were used as characterization techniques. An effective deposition on the textile surface due to ionic interactions with alternation of up to 10 layers of each of both polyelectrolytes was clearly evidenced. However, we observed that layer formation occurred to a lesser extent when TBBA/polyCTR-βCD complex was applied instead of polyCTR-βCD alone. The release study showed that drug reservoir properties and release kinetics could be controlled by the number of layers in the system and that TBBA release was faster than the multilayered coating degradation.
Chemical Communications | 2009
Julien Bigot; Marc Bria; Stuart T. Caldwell; Frédéric Cazaux; Alan Cooper; Bernadette Charleux; Graeme Cooke; Brian Fitzpatrick; David Fournier; Joël Lyskawa; Margaret Nutley; François Stoffelbach; Patrice Woisel
We describe the application of the LCST of a naphthalene-functionalised polyNIPAM derivative as a convenient, tuneable and reversible method to disrupt complex formation with CBPQT(4+) in water.
Carbohydrate Polymers | 2013
Maria José Garcia-Fernandez; Nicolas Tabary; Bernard Martel; Frédéric Cazaux; A. Oliva; Pablo Taboada; Angel Concheiro; Carmen Alvarez-Lorenzo
Efficient ophthalmic therapy requires the development of strategies that can provide sufficiently high drug levels in the ocular structures for a prolonged time. This work focuses on the suitability of poly-(cyclo)dextrins as carriers able to solubilize the carbonic anhydrase inhibitor (CAI) ethoxzolamide (ETOX), which is so far used for oral treatment of glaucoma. Topical ocular treatment should notably enhance the efficiency/safety profile of the drug. Natural α-, β- and γ-cyclodextrins and a maltodextrin were separately polymerized using citric acid as cross-linker agent under mild conditions. The resultant hydrophilic polymers exhibited larger capability to solubilize ETOX than the pristine (cyclo)dextrins. Moreover, they provided sustained drug diffusion in artificial lachrymal fluid. Interestingly the poly-(cyclo)dextrins solutions facilitate the loading of remarkably high doses of ETOX in poly(2-hydroxyethyl methacrylate)-based contact lenses. Exploiting ionic interactions between functional groups in the contact lenses and remnant free carboxylic acids in the citric acid linkers of poly-(cyclo)dextrins led to the retention of the drug-loaded poly-(cyclo)dextrins and, in turn, to sustained release for several weeks.
Polymer Chemistry | 2010
Julien Bigot; David Fournier; Joël Lyskawa; Thomas Marmin; Frédéric Cazaux; Graeme Cooke; Patrice Woisel
A series of poly(N-isopropylacrylamide)s (poly(NIPAM)s) have been synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization from a functionalized chain transfer agent (CTA) bearing either dialkoxynaphthalene or dialkoxyphenylene moieties. After demonstrating the controlled character of the RAFT polymerization in the presence of these CTAs, well-defined functionalized poly(NIPAM)s with low PDIs and similar molecular weights were selected and subjected to lower critical solution temperature (LCST) measurements using UV-vis spectroscopy. We have investigated the complexation of the polymers with the tetracationic cyclophane cyclobis(paraquat-p-phenylene) (CBPQT4+) and specifically the role the counter anion (Cl−, Br−, I−) of the cyclophane has on the LCST. Moreover, we have shown that the addition of a competing end-functionalized naphthalene poly(NIPAM) guest to a (CBPQT4+)–end-functionalized phenylene poly(NIPAM) complex results in the dethreading of the original architecture.
Journal of Biomedical Materials Research Part A | 2016
Jatupol Junthip; Nicolas Tabary; Feng Chai; Laurent Leclercq; Mickael Maton; Frédéric Cazaux; Christel Neut; Laurent Paccou; Yannick Guinet; Jean-Noel Staelens; Marc Bria; David Landy; Alain Hédoux; Nicolas Blanchemain; Bernard Martel
The coating of a nonwoven textile by polyelectrolyte multilayer film (PEM) issued from cationic and anionic β-cyclodextrin (βCD) polyelectrolytes according to the layer-by-layer (LbL) technique was successfully attempted. The tert-butyl benzoic acid (TBBA) was used as drug model to evaluate the loading capacity and sustained release properties of this PEM system. The build-up of the multilayer assembly was monitored in situ by optical waveguide lightmode spectroscopy (OWLS) on the one hand, and was assessed by gravimetry on the other hand when applied onto the textile substrate. In parallel, the complexation study of TBBA with both CD polyelectrolytes was also investigated by nuclear magnetic resonance (NMR) and isothermal titration calorimetry (ITC). The influence of thermal crosslinking of the multilayered coating on its stability and on TBBA release kinetics in phosphate buffered saline (PBS) at 37°C was studied. Finally, biological and microbiological tests were performed to investigate the cytocompatibility and the intrisic antibacterial activity of multilayer assemblies.
International Journal of Pharmaceutics | 2016
Safa Ouerghemmi; Stéphanie Degoutin; Nicolas Tabary; Frédéric Cazaux; Mickael Maton; Valérie Gaucher; Ludovic Janus; Christel Neut; Feng Chai; Nicolas Blanchemain; Bernard Martel
This work focuses on the relevance of antibacterial nanofibers based on a polyelectrolyte complex formed between positively charged chitosan (CHT) and an anionic hydroxypropyl betacyclodextrin (CD)-citric acid polymer (PCD) complexing triclosan (TCL). The study of PCD/TCL inclusion complex and its release in dynamic conditions, a cytocompatibility study, and finally the antibacterial activity assessment were studied. The fibers were obtained by electrospinning a solution containing chitosan mixed with PCD/TCL inclusion complex. CHT/TCL and CHT-CD/TCL were also prepared as control samples. The TCL loaded nanofibers were analyzed by Scanning Electron Microscopy (SEM), Fourier Transformed Infrared spectroscopy (FTIR) and X-Ray Diffraction (XRD). Nanofibers stability and swelling behavior in aqueous medium were pH and CHT:PCD weight ratio dependent. Such results confirmed that CHT and PCD interacted through ionic interactions, forming a polyelectrolyte complex. A high PCD content in addition to a thermal post treatment at 90°C were necessary to reach a nanofibers stability during 15days in soft acidic conditions, at pH=5.5. In dynamic conditions (USP IV system), a prolonged release of TCL with a reduced burst effect was observed on CHT-PCD polyelectrolyte complex based fibers compared to CHT-CD nanofibers. These results were confirmed by a microbiology study showing prolonged antibacterial activity of the nanofibers against Escherichia coli and Staphylococcus aureus. Such results could be explained by the fact that the stability of the polyelectrolyte CHT-PCD complex in the nanofibers matrix prevented the diffusion of the PCD/triclosan inclusion complex in the supernatant, on the contrary of the similar system including cyclodextrin in its monomeric form.
International Journal of Pharmaceutics | 2016
Maria José Garcia-Fernandez; Nicolas Tabary; Feng Chai; Frédéric Cazaux; Nicolas Blanchemain; M.P. Flament; Bernard Martel
A β-cyclodextrin (β-CD) polymer obtained by crosslinking β-CD with citric acid in its water-insoluble (PCD-I) and soluble (PCD-S) forms was used as a multifunctional direct compression excipient for tablet designing. PCD-I powder was obtained after grinding the solid fraction through a 200μm grid. PCD-S powder was recovered after lyophilization or spray drying of the PCD-S aqueous solutions, eventually followed by a wet granulation step. Both PCD-I and PCD-S powders were characterized, separately and mixed in variable ratios, based on dynamic water vapor sorption, SEM, particle size distribution, tapped density, compressibility, and flowability. PCD-I and spray dried and lyophilized/wet granulated PCD-S, as well as the mixture PCD-I/PCD-S=90/10, presented optimal free flowing characteristics. Then, PCD-I or PCD-S powders - separately or mixed in variable ratios - were used for tablets preparation by direct compression without adding any other excipient (e.g. binder, lubricant, disintegrant etc). As PCD-I decreased, tablets resistance to crushing and disintegration time increased from 15s to 15min (against 30min for β-CD), showing the improved disintegrant functionality of PCD-I, that rapidly swelled once in contact with water. Finally, PCD was force-fed to Sprague-Dawley rats (2g/kg) which were then observed during 14days for any clinical signs of toxicity.
Carbohydrate Polymers | 2017
Claudia Flores; Marco Lopez; Nicolas Tabary; Christel Neut; Feng Chai; Didier Betbeder; Clément Herkt; Frédéric Cazaux; Valérie Gaucher; Bernard Martel; Nicolas Blanchemain
Chitosan (CS) presents antibacterial, mucoadhesive and hemostatic properties and is an ideal candidate for wound dressing applications. This work reports the development of sponge-like materials obtained from physical hydrogels after the interaction between CS and a β-cyclodextrin polymer (PCD) in acidic conditions to provoke immediate gelation. Characterization consisted of zeta potential (ZP) measurements, rheology analysis, Fourier transform infrared (FTIR), Raman spectroscopy, wide angle X-ray scattering (WAXS) and scanning electron microscopy (SEM). Swelling behavior, cytotoxicity, drug sorption and drug delivery properties of sponges were assessed. ZP indicated that CS and PCD presented opposite charges needed for physical crosslinking. Rheology, swelling, and cytotoxicity of sponges depended on their CS:PCD weight ratios. Increasing PCD in the mixture delayed the gel time, reduced the swelling and increased the cytotoxicity. FTIR and Raman confirmed the physical crosslinking between CS and PCD through ionic interactions, and WAXS showed the amorphous state of the sponges. Finally, the efficiency of chlorhexidine loaded sponge against S. aureus bacteria was proved for up to 30days in agar diffusion tests.
Journal of the American Ceramic Society | 2017
Yao Zhang; Catherine A. Davy; Gregory Tricot; Cyrille Albert-Mercier; Natacha Henry; Pieter Bertier; Frédéric Cazaux; Denis Damidot; Xavier Bourbon