Frederick J. Hanke

Buzonamine, a new alkaloid from the defensive secretion of the millipede, Buzonium crassipes.

GC-MS analysis of the defensive secretion from the millipede Buzonium crassipes showed three volatile components, beta-pinene (35%), limonene (6%) and a new alkaloid, buzonamine (59%). Buzonamine had an HRMS molecular ion at m/z=221.1785 (calculated for C(14)H(23)NO, 221.1781), 14 carbons in the 13C-NMR and 23 hydrogens by SFORD, DEPT and APT experiments. All distinct 1H nuclei were assigned to 13C resonances with 2D 1H-13C COSY data, and the final structure was determined by 1H-1H COSY, 1H-13C HMBC and nOe experiments. Buzonamine contains four rings including an epoxy group and a tertiary nitrogen. beta-Pinene, limonene or buzonamine, kept the mound nesting ant, Formica obscuripes, from eating mealworms (Tenebrio molitor) treated with 1mg of the chemical. A 150mg millipede expels 4mg of secretion.

Epicatechin can cause the seedling growth inhibitor, nagilactone E, to induce growth stimulation

A possible new role for the flavonoid (−)-epicatechin (II) is described. It has no growth effects on its own, but when it is added to lettuce and rice seeds together with the known seedling growth inhibitor nagilactone E (I), the growth inhibitor activity ofI can cease and growth stimulation can be observed.

Droplet Countercurrent Chromatography: Recent Applications in Natural Products Chemistry

Abstract A few recent examples of our own modifications, procedures, and experiences utilizing DCCC are discussed that have enabled us to use DCCC more efficiently

Increasing the speed of droplet counter-current chromatography separations

Un systeme de vannes permet de traiter simultanement des groupes de colonnes, ce qui limite le parcours de solvant, diminue la pression de travail, entrainant une diminution de 30% des durees de lavage et chargement des colonnes