Fu Zhenyan

e0080 The CYP2J2 G50T polymorphism and the risk of coronary artery disease in Han Chinese population

Objective CYP2J2 is the major enzyme responsible for the formation of epoxyeicosatrienoic acids (EETs) in the heart, the EETs are potent endogenous vasodilators and inhibitors of vascular inflammation. The CYP2J2 G-50T polymorphism has been shown to be associated with increased risk of coronary artery disease (CAD) via lower plasma concentrations of EETs, while there are currently some population-based studies which found controversial results. The aim of the study was to assess associations between the G-50T polymorphism of CYP2J2 and CAD via a case-control study in the Han population of China. Methods There were 249 CAD patients and 243 age-matched control subjects genotyped for the CYP2J2 G-50T polymorphism. The data were assessed for three separate groups: the total subjects, men and women. Results In total, the distribution of the dominant model of G-50T promoter (G/G versus GT+TT) was significantly lower in CAD patients than control subjects (p=0.048), and simultaneously the T allele was significantly lower in CAD patients than control subjects (p=0.037). However, logistic regression analysis failed to show that the GT+TT genotype was a protective factor for CAD (OR 0.477, 95% CI 0.175 to 1.299; p=0.148). We investigated the synergistic effect between G/T+T/T genotype and no smoking or no DM, finally results showed G/T+T/T genotype and no smoking had a tendency to be a synergistic effect (G/T+T/T genotype+no smoking OR=0.296 vs G/T+T/T genotype+smoking OR=0.730), while lacked sufficient statistical power (p=0.127, p=0.663, respectively). Conclusions In presence of other risk factors, the CYP2J2 G-50T failed to show a significant association with coronary artery disease in Han population of China. However, since our result is close to the border of significance, further research based on the larger, prospective researches are necessary.

e0081 Association of genetic polymorphisms of SAA1 and SAA2 with coronary artery disease in Chinese Han population

Background Both Low plasma HDL cholesterol (HDL-C) and inflammatory responses are associated with an increased risk of coronary artery disease (CAD). Serum amyloid A protein (SAA) is not only an inflammatory factor but also an apolipoprotein that can replace apolipoprotein A1 (apoA1) as the major apolipoprotein of HDL. However, the relationship between genetic polymorphisms of SAA and CAD remains unclear. Methods 4 Single Nucleotide Polymorphisms (SNPs) (rs12218, rs1059559, rs2229338, and rs2468844) of SAA1 and SAA2 gene were genotyped in 1580 CAD patients and 1914 age- and sex-matched controls by the use of PCR-restriction fragment length polymorphism (PCR-RFLP) analysis. Results The CC genotype and C allele of rs12218 and the GG genotype and G allele of rs2468844 were more common in the CAD patients than in the control subjects, respectively (all p<0.001). After adjusted for diabetes mellitus, hypertension, smoking, drinking and lipid disorders by use of logistic regression, the SNPs rs12218 (OR=5.906, 95% CI 2.877 to 12.124, p<0.001) and rs2468844 (OR=4.102, 95% CI 2.018 to 8.129, p<0.001) still differed significantly between the CAD patients and control subjects. Conclusion These data suggest that genetic polymorphisms of SAA1/2 gene significantly increased the risk of CAD in a Chinese Han population.

e0254 Polymorphisms in the SAA12 gene are associated with carotid intima media thickness in Chinese Han healthy subjects

Aims Serum amyloid A protein (SAA) is not only an inflammatory factor, but also an apolipoprotein that can replace apolipoprotein A1 (apoA1) as the major apolipoprotein of HDL, which has been linked to atherosclerosis. However, the relationship between genetic polymorphisms and common carotid artery intima-media thickness (IMT) in healthy subjects remains unclear. We investigated the role of the SAA1 and SAA2 gene polymorphisms with IMT in a cohort of healthy subjects participating in the Cardiovascular Risk Survey (CRS) study. Methods Anthropometric and B-mode ultrasound of the carotid IMT were measured in 1914 subjects (849 men; 1065 women) recruited from 7 cities in Xinjiang, the west China. Four SNPs (rs12218, rs2229338, rs1059559, and rs2468844) were genotyped by use of PCR—restriction fragment length polymorphism (PCR-RFLP) method. Results There was significant difference between mutational genotype (CC+CT genotype) and wild genotype (TT genotype) of rs12218 in carotid IMT [(0.070±0.03) cm vs (0.084±0.03) cm; p<0.001)], and the difference remains significant after multi-adjusted (adjusted for the sex, age, blood pressure, BMI and HDL) [(0.068±0.002 cm vs 0.081±0.003 p =0.008)]. This relationship was also observed in rs246 8844 after multi-adjusted but did not found in rs222 9338 and rs105 9559 before and after multi-adjusted. Conclusions Both rs12218 of the SAA1 gene and rs246 8844 of SAA2 gene are associated with carotid IMT in healthy Chinese Han subjects.

e0255 Relationship of alcohol consumption and carotid atherosclerosis in Chinese Han Uighur and Hazakh cohort

Aim The relationship between alcohol consumption and carotid atherosclerosis had been reported in some epidemiologic studies. But the results were conflicting in different researches. In the present study, we investigated the association between alcohol intake and carotid atherosclerosis in Chinese Han, Uighur, and Hazakh population. Methods and Results The study population sample comprised 13 037 Chinese people (5277 Han, 4572 Uighur, and 3188 Hazakh) aged 35 years and over, who participated a Cardiovascular Risk Survey between June 2007 and September 2009. Daily alcohol consumption was determined by the number and frequency of alcoholic beverages consumed. The carotid artery parameters including common carotid artery intima-media thickness (CCA-IMT) and carotid plaques were measured using high-resolution B-mode ultrasonography. In Han or Hazakh, carotid IMT as a function of alcohol consumption was depicted as a J-shaped curve with a nadir for the alcohol intake category of 20 to 29.9 g/d; In Uighur, the similar curve with a nadir of 30 to 49.9 g/d was observed. For the prevalence of carotid plaques, we also observed the similar curves in Han and Hazakh but not in Uighur. After adjusted for the age, sex, blood pressure, body mass index, smoking, GLU, total cholesterol, HDL, and LDL, the J-shaped curves remain existence. Conclusions Our results indicated that moderate drinking is a protective factor for carotid atherosclerosis. But the definition of moderate drinking should be difference in Han, Uighur and Hazakh population.