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Dive into the research topics where Fumio Nomura is active.

Publication


Featured researches published by Fumio Nomura.


Clinical Cancer Research | 2004

Identification of Altered Protein Expression and Post-Translational Modifications in Primary Colorectal Cancer by Using Agarose Two-Dimensional Gel Electrophoresis

Takeshi Tomonaga; Kazuyuki Matsushita; Seiko Yamaguchi; Masamichi Oh-Ishi; Yoshio Kodera; Tadakazu Maeda; Hideaki Shimada; Takenori Ochiai; Fumio Nomura

Purpose: Although numerous proteome studies have been performed recently to identify cancer-related changes in protein expression, only a limited display of relatively abundant proteins has been identified. The aim of this study is to identify novel proteins as potential tumor markers in primary colorectal cancer tissues using a high-resolution two-dimensional gel electrophoresis (2-DE). Experimental Design: 2-DE using an agarose gel for isoelectric focusing was used to compare protein profiling of 10 colorectal cancer tissues and adjacent normal mucosa. Altered expression and post-translational modification of several proteins were examined using Western blot analysis and immunohistochemistry. Results: Ninety-seven proteins of 107 spots (90.7%) that were differentially expressed between matched normal and tumor tissues were identified by mass spectrometry. Among them, 42 unique proteins (49 spots) significantly increased or decreased in the tumors. They include eukaryotic translation initiation factor 4H, inorganic pyrophosphatase, anterior gradient 2 homologue, aldolase A, and chloride intracellular channel 1, whose elevated expression in tumor tissues was confirmed by Western blot analysis and immunohistochemistry. Interestingly, only isoform 1 of two transcript variants of eukaryotic translation initiation factor 4H was greatly up-regulated in most of the tumor tissues. Moreover, post-translational modifications of the prolyl-4-hydroxylase β subunit and annexin A2 also were identified. Conclusions: We identified several novel proteins with altered expression in primary colorectal cancer using agarose 2-DE. This method is a powerful technique with which to search for not only quantitative but also qualitative changes in a biological process of interest and may contribute to the deeper understanding of underlying mechanisms of human cancer.


Archive | 2003

Method and kit for diagnosing cancer

Hideaki Shimada; Takeshi Tomonaga; Kazuyuki Matsushita; Takenori Ochiai; Fumio Nomura


Archive | 2008

Marker peptide for diagnosis of alcoholic liver disease, and method for diagnosis of alcoholic liver disease using the same

Kazuyuki Sogawa; Fumio Nomura; Takeshi Tomonaga; Mamoru Sato; Tatsuya Ohashi; Iwao Kiyokawa; Ryo Kojima; Katsuhiro Katayama


Archive | 2005

Kit for solid cancer diagnosis and medicine for solid cancer therapy

Hideaki Shimada; Takeshi Tomonaga; Takaki Hiwasa; Kazuyuki Matsushita; Takenori Ochiai; Fumio Nomura; Masaki Takiguchi


Archive | 2008

Colorectal cancer marker polypeptide, and method for diagnosis of colorectal cancer

Yoshio Kodera; Tadakazu Maeda; Yusuke Kawashima; Fumio Nomura; Takeshi Tomonaga


The Japanese journal of clinical pathology | 2007

Novel tumor marker discovery of gastrointestinal cancers using various proteomic approaches

Takeshi Tomonaga; Fumio Nomura


Archive | 2004

Human solid cancer antigen peptides, polynucleotides encoding the same and utilization thereof

Hideaki Shimada; Takaki Hiwasa; Takeshi Tomonaga; Kazuyuki Matsushita; Fumio Nomura; Masaki Takiguchi; Takenori Ochiai


The Japanese journal of clinical pathology | 2013

Usefulness of the standardized serum cystatin C reagent in evaluation of renal function

Yoshitake Suzuki; Seimiya M; Toshihiko Yoshida; Yuji Sawabe; Kazuyuki Matsushita; Fumio Nomura


Archive | 2007

METHOD OF DETECTING CANCER USING SPLICING VARIANT OF c-myc GENE TRANSCRIPTIONAL REGULATOR FIR OR FOUR-BASE REPETITIVE SEQUENCE IN INTRON 2

Hideaki Shimada; Kazuyuki Matsushita; Takeshi Tomonaga; Fumio Nomura; Takenori Ochiai


Archive | 2006

Oncogene and diagnostic kit making use of the same

Hideaki Shimda; Takeshi Tomonaga; Kazuyuki Matsushita; Takenori Ochiai; Fumio Nomura

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