Fumio Nomura
Kitasato University
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Publication
Featured researches published by Fumio Nomura.
Clinical Cancer Research | 2004
Takeshi Tomonaga; Kazuyuki Matsushita; Seiko Yamaguchi; Masamichi Oh-Ishi; Yoshio Kodera; Tadakazu Maeda; Hideaki Shimada; Takenori Ochiai; Fumio Nomura
Purpose: Although numerous proteome studies have been performed recently to identify cancer-related changes in protein expression, only a limited display of relatively abundant proteins has been identified. The aim of this study is to identify novel proteins as potential tumor markers in primary colorectal cancer tissues using a high-resolution two-dimensional gel electrophoresis (2-DE). Experimental Design: 2-DE using an agarose gel for isoelectric focusing was used to compare protein profiling of 10 colorectal cancer tissues and adjacent normal mucosa. Altered expression and post-translational modification of several proteins were examined using Western blot analysis and immunohistochemistry. Results: Ninety-seven proteins of 107 spots (90.7%) that were differentially expressed between matched normal and tumor tissues were identified by mass spectrometry. Among them, 42 unique proteins (49 spots) significantly increased or decreased in the tumors. They include eukaryotic translation initiation factor 4H, inorganic pyrophosphatase, anterior gradient 2 homologue, aldolase A, and chloride intracellular channel 1, whose elevated expression in tumor tissues was confirmed by Western blot analysis and immunohistochemistry. Interestingly, only isoform 1 of two transcript variants of eukaryotic translation initiation factor 4H was greatly up-regulated in most of the tumor tissues. Moreover, post-translational modifications of the prolyl-4-hydroxylase β subunit and annexin A2 also were identified. Conclusions: We identified several novel proteins with altered expression in primary colorectal cancer using agarose 2-DE. This method is a powerful technique with which to search for not only quantitative but also qualitative changes in a biological process of interest and may contribute to the deeper understanding of underlying mechanisms of human cancer.
Archive | 2003
Hideaki Shimada; Takeshi Tomonaga; Kazuyuki Matsushita; Takenori Ochiai; Fumio Nomura
Archive | 2008
Kazuyuki Sogawa; Fumio Nomura; Takeshi Tomonaga; Mamoru Sato; Tatsuya Ohashi; Iwao Kiyokawa; Ryo Kojima; Katsuhiro Katayama
Archive | 2005
Hideaki Shimada; Takeshi Tomonaga; Takaki Hiwasa; Kazuyuki Matsushita; Takenori Ochiai; Fumio Nomura; Masaki Takiguchi
Archive | 2008
Yoshio Kodera; Tadakazu Maeda; Yusuke Kawashima; Fumio Nomura; Takeshi Tomonaga
The Japanese journal of clinical pathology | 2007
Takeshi Tomonaga; Fumio Nomura
Archive | 2004
Hideaki Shimada; Takaki Hiwasa; Takeshi Tomonaga; Kazuyuki Matsushita; Fumio Nomura; Masaki Takiguchi; Takenori Ochiai
The Japanese journal of clinical pathology | 2013
Yoshitake Suzuki; Seimiya M; Toshihiko Yoshida; Yuji Sawabe; Kazuyuki Matsushita; Fumio Nomura
Archive | 2007
Hideaki Shimada; Kazuyuki Matsushita; Takeshi Tomonaga; Fumio Nomura; Takenori Ochiai
Archive | 2006
Hideaki Shimda; Takeshi Tomonaga; Kazuyuki Matsushita; Takenori Ochiai; Fumio Nomura