G. G. Wendt

Beitrag zur Problematik des Lp-Systems

It is demonstrated that the Lp(a) positive reactions can be divided into strong positive (Lp(a+)) and weak positive (Lp(as+)) types. Frequency among healthy and sick persons, age distribution, and inheritance of both factors were investigated.The significance of these findings for the difficulties of the Lp-sytem is discussed.ZusammenfassungEs wird gezeigt, daß die positiven Lp(a)-Reaktionen sich in stark positive (Lp(a+)) und schwach positive (Lp(as+)) unterteilen lassen. Frequenz bei Gesunden und Kranken, Altersverteilung und Vererbung beider Faktoren werden untersucht.Die Bedeutung dieser Befunde für die Problematik des Lp-Systems wird diskutiert.

Das Lp-System

Rabbits are immunized with purified human serum fl-Lipoprotein from one single individual. This individual has to be Lp(a-/-). l~abbig immune serum produced in this way contains antibodies against all the fl-Lipoproteins. If it is absorbed with human serum from an individual which is Lp(a--), it is possible to obtain a specific immune serum against the factor Lp(a+). The method to obtain anti-Lp(a)-serum and the technique of Lp-identification are described. The frequency of the gene Lp a (301 ~ ~~ ) is 0.179. Relations between the Lp-system and other genetic systems of the blood with possible exception of the factor Gc (1--1) could not be found.

Auftrennung von AK (EC 2.7.4.3) und ADA (EC 3.5.4.4) in einem Strkeblock

SummaryA new method is described for simultaneous starch gel electrophoresis of AK and ADA.

Beitrag zur Populationsgenetik der Glutathionreduktase menschlicher Erythrocyten

SummaryThe frequency of heterozygotes for the glutathione-reductase in a sample of 215 young healthy Germans is 1,9%. Suggesting a 2-allele-model we can calculate the frequency of the three phenotypes in the population: GR(A)=98,1%, GR(AB)=1,9%, GR(B)=0,009%. No correlation between blood-and serum-groups and the enzyme activity could be found.

A faster migrating Gc-variant: Gc Darmstadt

SummaryIn three members of a family from Darmstadt (Germany) a faster migrating Gc variant has been observed. The variant phenotypes have been examined by routine immunoelectrophoresis (Fig. 1), by immunoelectrophoresis with prolonged separation times and with Gc-monospecific antisera (Fig. 2), by polyacrylamide gel electrophoresis (Fig. 3), and by antigen-antibody crossed electrophoresis (Fig. 4). By antigen-antibody crossed electrophoresis the new Gc variant was clearly distinguishable from the Gc Aborigine and from the Gc Chippewa variant. The variant was named Gc Darmstadt (Gc D). Gc Darmstadt has an electrophoretic migration rate intermediate between Gc Ab and Gc 1. In two sibs the type Gc D-2 was observed, the daughter of one of these sibs had the type Gc D-1. The analysis of several members of this family provided only limited information on the mode of inheritance of Gc Darmstadt (Fig. 5). Gc Darmstadt appears to be determined by a gene GcD which may be allelic to Gc1 and Gc2.ZusammenfassungBei drei Angehörigen einer Familie aus Darmstadt (Deutschland) wurde eine schneller wandernde Gc-Variante beobachtet. Die neue Variante, die eindeutig von Gc Aborigine und Gc Chippewa unterschieden werden kann, wurde Gc Darmstadt (Gc D) genannt. Bei elektrophoretischer Auftrennung liegt Gc Darmstadt zwischen Gc Ab und Gc 1. Gc Darmstadt ist sehr wahrscheinluch durch ein Gen GcD bedingt, das ein Allel zu Gc1 und Gc2 ist.

Beitrag zur Populationsgenetik der Pyruvatkinase menschlicher Erythrocyten

SummaryIn 214 healthy young Germans the activity of Pyruvatekinase from red blood cells has been determined. Three persons had values in the heterozygote range between 10.0 and 20.0 U. Suggesting a 2-allele-model the frequency of the three phenotypes in the German population can be calculated as followed: PK(A)=98.6%, PK(AB)=1.4%, PK(B)=0.005%.No correlation could be found between the distribution of blood-and serum-groups and the enzyme-activity.

Statistische Betrachtung ber die Hufigkeit der Haptoglobin-Typen bei verschiedenen Krankheiten

The frequency of the three most common haptoglobin phenotypes are studied in a sample of 2160 persons which are divided in a control group and groups of patients suffering of cardiac infarction, psoriasis, colhim cancer, eholelithiasis, gastric ulcer, and neurodermitis; all groups coming from the same North Hassian population. The overall-z2-test gives no significant inhomogenity. The pairwise comparison with the z2-test gives no significant difference between disease and control groups. A significant difference (P ~ 0,04) has been found between the cardial infarctus and the collum cancer group. I t may, however, be discussed wether in the case of such multiple tests the significance level of the usual z2-distribution can be used instead of that from the (still unknown) distribution of the maximal g 2. Finally WooLFs method (1955) is compared with the )/e-test.

Zur populationsgenetik der phosphoglucoseisomerase (EC: 5.3.1.9)

SummaryIn a population sample of 728 unrelated individuals from Marburg one variant PGI 5-1 was found.

Problematischer Mutter-Kind-Ausschluß mit PGM1

SummaryAn apparently false exclusion of maternity (PGM11 versus PGM12) indicates the possibility of an additional allele of the PGM1 locus, which cannot be detected by the usual electrophoretic methods.

A pilot scheme for a genetic clinic

SummaryReport on a special genetic counselling centre with 1152 cases within 1 year.ZusammenfassungEs wird über den Modellversuch mit einer speziellen genetischen Beratungsstelle berichtet. Die 1152 Fälle des ersten Jahres werden diagnostisch aufgegliedert.