G. P. Walker

Effects of nutrition and management on the production and composition of milk fat and protein: a review

The composition and functional properties of cow’s milk are of considerable importance to the dairy farmer, manufacturer, and consumer. Broadly, there are 3 options for altering the composition and/or functional properties of milk: cow nutrition and management, cow genetics, and dairy manufacturing technologies. This review considers the effects of nutrition and management on the composition and production of milk fat and protein, and the relevance of these effects to the feeding systems used in the Australian dairy industry. Dairy cows on herbage-based diets derive fatty acids for milk fat synthesis from the diet/rumen microorganisms (400–450 g/kg), from adipose tissues (<100 g/kg), and from de novo synthesis in the mammary gland (about 500 g/kg). However, the relative contributions of these sources of fatty acids to milk fat production are highly dependent upon feed intake, diet composition, and stage of lactation. Feed intake, the amount of starch relative to fibre, the amount and composition of long chain fatty acids in the diet, and energy balance are particularly important. Significant differences in these factors exist between pasture-based dairy production systems and those based on total mixed ration, leading to differences in milk fat composition between the two. High intakes of starch are associated with higher levels of de novo synthesis of fat in the mammary gland, resulting in milk fat with a higher concentration of saturated fatty acids. In contrast, higher intakes of polyunsaturated fatty acids from pasture and/or lipid supplements result in higher concentrations of unsaturated fatty acids, particularly oleate, trans-vaccenate, and conjugated linoleic acid (CLA) in milk fat. A decline in milk fat concentration associated with increased feeding with starch-based concentrates can be attributed to changes in the ratios of lipogenic to glucogenic volatile fatty acids produced in the rumen. Milk fat depression, however, is likely the result of increased rates of production of long chain fatty acids containing a trans-10 double bond in the rumen, in particular trans-10 18 : 1 and trans-10-cis-12 18 : 2 in response to diets that contain a high concentration of polyunsaturated fatty acids and/or starch. Low rumen fluid pH can also be a factor. The concentration and composition of protein in milk are largely unresponsive to variation in nutrition and management. Exceptions to this are the effects of very low intakes of metabolisable energy (ME) and/or metabolisable protein (MP) on the concentration of total protein in milk, and the effects of feeding with supplements that contain organic Se on the concentration of Se, as selenoprotein, in milk. In general, the first limitation for the synthesis of milk protein in Australian dairy production systems is availability of ME since pasture usually provides an excess of MP. However, low concentrations of protein in milk produced in Queensland and Western Australia, associated with seasonal variations in the nutritional value of herbage, may be a response to low intakes of both ME and MP. Stage of lactation is important in determining milk protein concentration, but has little influence on protein composition. The exception to this is in very late lactation where stage of lactation and low ME intake can interact to reduce the casein fraction and increase the whey fraction in milk and, consequently, reduce the yield of cheese per unit of milk. Milk and dairy products could also provide significant amounts of Se, as selenoproteins, in human diets. Feeding organic Se supplements to dairy cows grazing pastures that are low in Se may also benefit cow health. Research into targetted feeding strategies that make use of feed supplements including oil seeds, vegetable and fish oils, and organic Se supplements would increase the management options available to dairy farmers for the production of milks that differ in their composition. Given appropriate market signals, milk could be produced with lower concentrations of fat or higher levels of unsaturated fats, including CLA, and/or high concentrations of selenoproteins. This has the potential to allow the farmer to find a higher value market for milk and improve the competitiveness of the dairy manufacturer by enabling better matching of the supply of dairy products to the demands of the market.

Influence of pasture and concentrates in the diet of grazing dairy cows on the fatty acid composition of milk

In five short-term experiments conducted in Victoria in 1997 and 1998, grazing dairy cows were given either pasture alone or pasture supplemented with high-energy concentrates, and the fatty acid profiles of milk fat were measured. We established the effects of these feeds on some aspects of milk fat of importance for human nutrition, but we specifically focused on the hypothesis that conjugated linoleic acid (CLA) concentrations in milk fat increase as pasture intake increases, and decrease as more concentrates are fed. In agreement with previous research, feeding fresh pasture alone resulted in high concentrations (1.0-1.8 g/100 g milk fat) of CLA. When the effect of level of pasture consumption on CLA content was examined, a significant positive relationship (r2 = 0.35; P < 0.05) was obtained. When cereal grain concentrates were used to supplement pasture intake, the CLA content of milk fat generally declined (P < 0.05), except where the amount of concentrates given led to a marked reduction in total milk fat concentration. The use of cereal grain concentrates also generally resulted in significant (P < 0.05) increases in medium-chain saturated fatty acids, but always reduced the contribution of butyric acid to milk fat, from 4.5 to 3.9 g/100 g milk fat, on average.

Rumen fermentation characteristics of dairy cows grazing different allowances of Persian clover- or perennial ryegrass-dominant swards in spring

An experiment was conducted in which cows in early lactation grazed Persian clover (Trifolium resupinatum L.) or perennial ryegrass (Lolium perenne L.)-dominant pastures at low or high pasture allowances in order to determine the effects of pasture type and level of feeding on rumen fermentation patterns. The hypotheses for grazing dairy cows were: (i) the consumption of Persian clover would result in a more rapid rate of degradation and less stable rumen fermentation patterns compared with perennial ryegrass; and (ii) the greater intake of cows grazing at high compared with low pasture allowances would also cause less stable rumen fermentation patterns. Stability of rumen fermentation refers to the level to which rumen fluid pH declines, especially for long periods of a day, indicating that the rumen is not coping with neutralising and/or removing acids. Cows grazing Persian clover had lower (P 0.05) of pasture allowance on the degradation rate of perennial ryegrass dry matter, but the higher allowance of Persian clover resulted in the highest (P<0.05) rate of degradation of dry matter compared with either ryegrass treatment or the low allowance of Persian clover. The effective dry matter degradability of Persian clover was greater (P<0.05) than that of perennial ryegrass, and the effective dry matter degradability of herbage in cows grazing at low allowances was greater (P<0.05) than at higher allowances. However, future research should consider neutral detergent fibre degradation in grazing dairy cows with low rumen fluid pH levels.

Profitable feeding of dairy cows on irrigated dairy farms in northern Victoria

Milk production per cow and per farm in the irrigated region in northern Victoria have increased dramatically over the past 2 decades. However, these increases have involved large increases in inputs, and average productivity gains on farms have been modest. Before the early 1980s, cows were fed predominantly pasture and conserved fodder. There is now large diversity in feeding systems and feed costs comprise 40–65% of total costs on irrigated dairy farms. This diversity in feeding systems has increased the need to understand the nutrient requirements of dairy cows and the unique aspects of nutrient intake and digestion in cows at grazing. Principles of nutrient intake and supply to the grazing dairy cow from the past 15 years’ research in northern Victoria are summarised and gaps in knowledge for making future productivity gains are identified. Moreover, since the majority of the milk produced in south-eastern Australia is used in the manufacture of products for export, dairy companies have increased their interest in value-added dairy products that better meet nutritional requirements or provide health benefits for humans. Finally, some examples of the impacts of farm system changes on operating profit for some case study farms in northern Victoria are presented to illustrate the need for thorough analysis of such management decisions.

Output of selenium in milk, urine, and feces is proportional to selenium intake in dairy cows fed a total mixed ration supplemented with selenium yeast

Fifteen rumen fistulated Holstein cows in late lactation and fed a total mixed ration offered ad libitum were supplemented with Se yeast to provide 0, 11, 20, 30, or 42 mg of supplemental Se/day to test the hypothesis that amounts of Se secreted in milk, excreted in urine and feces, and apparently retained in tissues would increase in direct proportion to Se intake. One-half of the yeast supplement was placed directly into the rumen through the fistula of each cow just before milking in the morning and again in the evening, and estimates of average daily excretion of Se were made using total collections of urine and feces from 25 to 31 d after treatments commenced. Amounts of Se secreted daily in milk and apparently retained in tissues increased linearly with average daily intake of Se. The amount of Se excreted in feces and total excretion of Se in urine plus feces increased curvilinearly with Se intake, such that proportionately less Se was excreted as the amount of Se fed increased. On average, total Se excretion accounted for 66%, Se secretion in milk accounted for 17%, and Se apparently retained in tissues accounted for 17% of total Se intake by cows. Thus, in herds fed large amounts of Se yeast, most of the Se will be excreted and retained on-farm. High concentrations of Se will be found where urine and feces accumulate (e.g., yards and effluent ponds), and effluent management practices must be tailored to avoid environmental issues.

Selenium levels in cows fed pasture and concentrates or a total mixed ration and supplemented with selenized yeast to produce milk with supra-nutritional selenium concentrations

Seventy multiparous Holstein-Friesian cows were fed different amounts of pasture and concentrates, or a total mixed ration (TMR), for 42 d in mid-lactation to test the hypothesis that the concentration of Se in milk would depend on the amount of Se consumed, when the Se is primarily organic in nature, regardless of the diet of the cows. Of the 70 cows, 60 grazed irrigated perennial pasture at daily allowances of either 20 or 40 kg of dry matter (DM)/cow. These cows received 1 of 3 amounts of concentrates, either 1, 3, or 6 kg of DM/cow per day of pellets, and at each level of concentrate feeding, the pellets were formulated to provide 1 of 2 quantities of Se from Se yeast, either about 16 or 32 mg of Se/d. The other 10 cows were included in 2 additional treatments where a TMR diet was supplemented with 1 kg of DM/d of pellets formulated to include 1 of the 2 quantities of supplemental Se. Total Se intakes ranged from 14.5 to 35.9 mg/d, and of this, the Se-enriched pellets provided 93, 91, and 72% of the Se for cows allocated 20 and 40 kg of pasture DM/d or the TMR, respectively. No effects of the amount of Se consumed on any milk production variable, or on somatic cell count, body weight, and body condition score, for either the pasture-fed or TMR-fed cows were found. Milk Se concentrations responded quickly to the commencement of Se supplementation, reaching 89% of steady state levels at d 5. When milk Se concentrations were at steady state (d 12 to 40), each 1mg of Se eaten increased the Se concentration of milk by 5.0 μg/kg (R(2)=0.97), and this response did not seem to be affected by the diet of the cows or their milk production. The concentration of Se in whole blood was more variable than that in milk, and took much longer to respond to change in Se status, but it was not affected by diet at any time either. For the on-farm production of Se-enriched milk, it is important to be able to predict milk Se concentration from Se input. In our study, type of diet did not affect this relationship.

Seasonal variation in the concentrations of conjugated linoleic and trans fatty acids in milk fat from commercial dairy farms is associated with pasture and grazing management and supplementary feeding practices

A study of irrigated pasture-based dairy farms that used split calving (autumn and spring) was undertaken in northern Victoria, Australia, to examine associations between nutrition, time of year and season of calving on the concentrations of isomers of trans 18 : 1 fatty acids and conjugated linoleic acids (CLA) in milk fat. Factors associated with time of year explained most of the variation, with the highest concentrations observed in spring and summer when pasture intake by herds was high. However, there was substantial variation observed between herds and time of year. The mean total CLA concentration was 9.1 mg/g milk fatty acids (range 1.1–35.4 mg/g) with the cis,trans-9,11 accounting for ~84% of the total CLA. The mean total trans 18 : 1 concentration was 60.5 mg/g milk fatty acids (range 13.6–267 mg/g) with vaccenic acid (trans-11 18 : 1) accounting for ~53% of total trans 18 : 1 fatty acids. Total CLA and vaccenic acid were highest in August–September (southern hemisphere spring) (15.1 and 76.3 mg/g milk fat) and lowest in November–March (5.6 mg/g milk fat) and May–July (9.53 mg/g milk fat), respectively. There was no association between season of calving and milk CLA or trans 18 : 1 fatty acid concentrations. Trans-10 and -11 18 : 1 fatty acids and trans/trans-CLA were negatively correlated with milk fat concentrations. Management strategies designed to increase the concentration of CLA and trans 18 : 1 fatty acids in milk fat would not need to consider the effects of season of calving or stage of lactation, but should focus on pasture availability and quality.

Seasonal variation in milk production and cheese yield from commercial dairy farms located in northern Victoria is associated with pasture and grazing management and supplementary feeding practices

A study of irrigated pasture-based commercial dairy farms that made use of split calving (two distinct periods of calving; autumn and spring) was undertaken between April 2001 and March 2002 in northern Victoria, to examine associations between herd nutrition, time of year and season of calving and the production and composition of milk. On average, herds that had access to higher digestibility pasture or were fed more cereal grain-based concentrates produced more milk. However, the average marginal yield of 4% fat corrected milk/kg cereal grain-based concentrates was less than responses achieved under experimental conditions in northern Victoria. Herds that calved in autumn had different production characteristics to those that calved in spring, in that they did not show an early lactation peak in milk yield and produced milk with lower average concentrations of crude protein, casein and fat. Despite this, herds that calved in autumn had greater persistency of milk yield in mid to late lactation, when they tended to be better fed on pasture, so that yields of milk solids over a notional 310-day lactation were similar for both calving groups (523 v. 529 kg fat + protein; autumn v. spring, respectively), but herds that calved in autumn produced milk with a lower potential to yield cheddar cheese (10.2 v. 10.6 kg cheese/100 kg milk; P < 0.01). Farms that produced milk in the lowest quartile for potential to yield cheddar cheese differed from the top quartile in that they: (i) milked fewer cows (175 v. 250); (ii) fed less supplements (5.6 v. 9.4 kg DM/cow.day); (iii) walked their herds shorter distances between pasture and the dairy (2.2 v. 3.2 km/day); (iv) allocated lower herbage allowances (33 v. 43 kg DM/cow.day); and (v) grazed pastures at a mass low enough to have restricted pasture intake (< 3 t DM/ha), about twice the frequency of farms (0.40 v. 0.17) in the top quartile. Greater productivity of the dairy industry in northern Victoria could be achieved through better grazing and pasture management and supplementary feeding practices on farms.

Seasonal and stage of lactation effects on milk fat composition in northern Victoria

Effect of herd nutrition, time of year and season of calving on milk fat composition and physical properties were examined on irrigated commercial dairy farms in northern Victoria that made use of split-calving and a diverse range of feeding systems. Twenty-four farms were included in the study, and from each farm, morning and evening milk samples were collected from 16 cows that calved in autumn and 16 cows that calved in spring. There were no significant effects of season of calving on the concentration of fatty acids or phospholipids in milk fat, but there were interactions between season of calving and time of year (P < 0.001). These differences could be attributed to changes in energy balance and body condition with stage of lactation. The phospholipids comprising mainly phosphotidylcholine (PC), phosphtidylethanolamine (PE) and sphingomyelin (SP) also varied, with PC and PE being highest in late lactation and SP lowest during peak and mid lactation for both calving groups. Milk fat colour and the concentrations of free fatty acids were more influenced by factors associated with time of year rather than stage of lactation. Milk fat colour in particular showed strong seasonal variation being distinctly lighter in summer–early autumn when compared with rest of the year. Increasing the amount of concentrates fed was associated with decreases in short-chain fatty acid concentration and increases in the solid fat content of milk fat. Variations in nutritional management practices had only small (non-significant) effects on fat composition.

Producing milk with uniform high selenium concentrations on commercial dairy farms

Six herds on five commercial dairy farms were involved in the production of high selenium (Se) milk. The farms had a range of herd sizes, herd structures, feeding systems and milk production per cow. On all farms, pelleted concentrate supplements containing Se yeast were fed twice daily in the dairy for 16 days. The objectives were to: (1) produce milk with Se concentrations exceeding 225 μg/kg on the five farms for pilot-scale production of a high protein milk powder; (2) validate a predictive relationship between Se intake and milk Se concentration developed in research; and (3) examine the time taken from the introduction of Se yeast to steady-state concentrations of Se in milk under a range of commercial farming conditions. We hypothesised that the relationship between Se intake and its concentration in milk found in research would apply on commercial farms. Daily Se intake, which was primarily from Se yeast in the pelleted concentrates, varied from 35 to 51 mg Se/cow. Grazed pasture and conserved forage contributed less than 1 mg Se/cow on all farms. The time taken from the introduction of pellets containing Se yeast to steady-state milk Se concentrations was 4–7 days. The steady-state Se concentrations in milk varied from 166 to 247 µg/kg, but these concentrations were only 55–72% of predicted values. All the milk produced from the five farms on the last 2 days of feeding of Se-enriched pellets was used to produce a milk protein concentrate with a Se concentration of 5.4 mg/kg. Factors that might have affected Se incorporation into milk and the implications of these results for commercial production of high Se milk or milk products are discussed.