G. Quero

Long period fiber grating nano-optrode for cancer biomarker detection

We report an innovative fiber optic nano-optrode based on Long Period Gratings (LPGs) working in reflection mode for the detection of human Thyroglobulin (TG), a protein marker of differentiated thyroid cancer. The reflection-type LPG (RT-LPG) biosensor, coated with a single layer of atactic polystyrene (aPS) onto which a specific, high affinity anti-Tg antibody was adsorbed, allowed the label-free detection of Tg in the needle washouts of fine-needle aspiration biopsies, at concentrations useful for pre- and post-operative assessment of the biomarker levels. Analyte recognition and capture were confirmed with a parallel on fiber ELISA-like assay using, in pilot tests, the biotinylated protein and HRP-labeled streptavidin for its detection. Dose-dependent experiments showed that the detection is linearly dependent on concentration within the range between 0 and 4 ng/mL, while antibody saturation occurs for higher protein levels. The system is characterized by a very high sensitivity and specificity allowing the ex-vivo detection of sub ng/ml concentrations of human Tg from needle washouts of fine-needle aspiration biopsies of thyroid nodule from different patients.

Label-free fiber optic optrode for the detection of class C β-lactamases expressed by drug resistant bacteria

This paper reports the experimental assessment of an automated optical assay based on label free optical fiber optrodes for the fast detection of class C β-lactamases (AmpC BLs), actually considered as one of the most important sources of resistance to β-lactams antibiotics expressed by resistant bacteria. Reflection-type long period fiber gratings (RT-LPG) have been used as highly sensitive label free optrodes, while a higher affine boronic acid-based ligand was here selected to enhance the overall assay performances compared to those obtained in our first demonstration. In order to prove the feasibility analysis towards a fully automated optical assay, an engineered system was developed to simultaneously manipulate and interrogate multiple fiber optic optrodes in the different phases of the assay. The automated system tested in AmpC solutions at increasing concentrations demonstrated a limit of detection (LOD) of 6 nM, three times better when compared with the results obtained in our previous work. Moreover, the real effectiveness of the proposed optical assay has been also confirmed in complex matrices as the case of lysates of Escherichia coli overexpressing AmpC.

Simple technique for integrating compact silicon devices within optical fibers

In this work, we present a simple fabrication process enabling the integration of a subwavelength amorphous silicon layer inside optical fibers by means of the arc discharge technique. To assess our method, we have fabricated a compact in-line Fabry-Perot interferometer consisting of a thin (<1  μm) a-Si:H layer completely embedded within a standard single-mode optical fiber. The device exhibits low loss (1.3 dB) and high interference fringe visibility (~80%) both in reflection and transmission, due to the high refractive index contrast between silica and a-Si:H. A high linear temperature sensitivity up to 106  pm/°C is demonstrated in the range 120°C-400°C. The proposed interferometer is attractive for point monitoring applications as well as for ultrahigh-temperature sensing in harsh environments.

Self-assembled periodic patterns on the optical fiber tip by microsphere arrays

In this work, we report a fabrication route for self-assembling periodic patterns on optical fiber tips. The technique is based on self-assembling polystyrene microspheres at the air/water interface and on successive transferring of the monolayer colloidal crystal on the fiber tip. By applying to the fiber further treatments like particle size reduction, metal coating and sphere removal, different periodic structures are conveniently realized. The results obtained indicate that self-assembly technique affords opportunity to create on the optical fiber tip dielectric and metallic-dielectric spheres’ arrays with a feature size down to a submicron scale or metallic patterns with a few hundred nanometers at low fabrication costs.

Lab on fiber by using the breath figure technique

The “Lab on Fiber” technology has been recently proposed as a valuable route for the realization of novel and highly functionalized technological platforms completely integrated in a single optical fiber in communication and sensing applications. As a follow up of the proposed technological approach, here, we present recent results on the fabrication of metallo-dielectric structures on the optical fiber tip by using a self-assembly technique. Our studies aim to attain advanced nanostructured sensors by exploiting easy and low cost fabrication processes suitable to be employed in massive production of technologically advanced devices. The pursued approach basically consists in the preliminary preparation of a patterned polymeric film by the breath figure technique, directly on the optical fiber tip, and in the successive metal deposition by evaporation. The experimental results demonstrate the successful creation of a metallodielectric honeycomb pattern on the optical fiber tip. The experimental spectral features are in good agreement with the numerical analysis, elucidating the photonic and plasmonic interactions occurring in the Lab onto the optical fiber tip. The sensing properties of the optical fiber probes have been successfully explored in terms of sensitivity to the surrounding refractive index changes demonstrating their potentialities for chemical and biological sensing applications.

Hybrid fiber grating cavity for multi-parametric sensing

We propose an all-fiber hybrid cavity involving two unbalanced uniform fiber Bragg gratings (FBGs) written at both sides of a tilted FBG (TFBG) to form an all-fiber interferometer. This configuration provides a wavelength gated reflection signal with interference fringes depending on the cavity features modulated by spectral dips associated to the wavelength dependent optical losses due to cladding mode coupling occurring along the TFBG. Such a robust structure preserves the advantages of uniform FBGs in terms of interrogation methods and allows the possibility of simultaneous physical and chemical sensing.

Lab-on-Fiber biosensing for cancer biomarker detection

This work deals with a novel Lab-on-Fiber biosensor able to detect in real time thyroid carcinomas biomarkers. The device is based on a gold nanostructure supporting localized surface plasmon resonances (LSPR) directly fabricated on the fiber tip by means of electron beam lithography and lift-off process. Following a suitable chemical and biological functionalization of the sensing area, human Thyroglobulin has been detected at nanomolar concentrations. Also, compatibility with full baseline restoration, achieved through biomarkers/bioreceptors dissociation, has been demonstrated.

Ultrasensitive nanoprobes based on metallo-dielectric crystals integrated onto optical fiber tips using the breath figures technique

We present recent results on the fabrication of metallo-dielectric structures on the optical fiber tip by using a self-assembly technique. Our studies aim to attain advanced nanostructured sensors by exploiting easy and low cost fabrication processes suitable to be employed in massive production of technologically advanced devices in the roadmap of the Lab on Fiber Technology. The pursued approach basically consists in the preliminary preparation of a patterned polymeric film by the breath figure technique, directly on the optical fiber tip, and in the successive metal deposition by evaporation. The experimental results demonstrate the successful creation of a metallo-dielectric honeycomb pattern on the optical fiber tip. The experimental spectral features are in good agreement with the numerical analysis, elucidating the photonic and plasmonic interactions occurring in the Lab onto the optical fiber tip. The sensing properties of the optical fiber probes have been successfully explored in terms of sensitivity to the surrounding refractive index changes demonstrating their potentialities for chemical and biological sensing applications.

Ultracompact optical fiber Fabry-Perot interferometer based on in-line integrated sub-micron silicon film

In this work, an ultra compact in line fiber optic Fabry-Perot interferometer is presented. The interferometric structure consists of a thin (< 1 μm) amorphous silicon layer in line integrated into a standard single mode optical fiber by means of an electric arc discharge technique. The device exhibits low loss (1.46 dB) and high interference fringe visibility (~ 30% in linear scale) both in reflection and transmission due to the high refractive index contrast between silica and α-Si. A high linear temperature sensitivity up to 75 pm/°C is demonstrated in the range 15-52 °C. The proposed device is simple, compact, cost effective and attractive for point monitoring sensing application in ultra-high temperature sensing in harsh environments.

Nanosphere Lithography on Fiber: Towards Engineered Lab-On-Fiber SERS Optrodes

In this paper we report on the engineering of repeatable surface enhanced Raman scattering (SERS) optical fiber sensor devices (optrodes), as realized through nanosphere lithography. The Lab-on-Fiber SERS optrode consists of polystyrene nanospheres in a close-packed arrays configuration covered by a thin film of gold on the optical fiber tip. The SERS surfaces were fabricated by using a nanosphere lithography approach that is already demonstrated as able to produce highly repeatable patterns on the fiber tip. In order to engineer and optimize the SERS probes, we first evaluated and compared the SERS performances in terms of Enhancement Factor (EF) pertaining to different patterns with different nanosphere diameters and gold thicknesses. To this aim, the EF of SERS surfaces with a pitch of 500, 750 and 1000 nm, and gold films of 20, 30 and 40 nm have been retrieved, adopting the SERS signal of a monolayer of biphenyl-4-thiol (BPT) as a reliable benchmark. The analysis allowed us to identify of the most promising SERS platform: for the samples with nanospheres diameter of 500 nm and gold thickness of 30 nm, we measured values of EF of 4 × 105, which is comparable with state-of-the-art SERS EF achievable with highly performing colloidal gold nanoparticles. The reproducibility of the SERS enhancement was thoroughly evaluated. In particular, the SERS intensity revealed intra-sample (i.e., between different spatial regions of a selected substrate) and inter-sample (i.e., between regions of different substrates) repeatability, with a relative standard deviation lower than 9 and 15%, respectively. Finally, in order to determine the most suitable optical fiber probe, in terms of excitation/collection efficiency and Raman background, we selected several commercially available optical fibers and tested them with a BPT solution used as benchmark. A fiber probe with a pure silica core of 200 µm diameter and high numerical aperture (i.e., 0.5) was found to be the most promising fiber platform, providing the best trade-off between high excitation/collection efficiency and low background. This work, thus, poses the basis for realizing reproducible and engineered Lab-on-Fiber SERS optrodes for in-situ trace detection directed toward highly advanced in vivo sensing.