Gail E. Bingham

Comparison of the Bowen ratio-energy balance and the water balance methods for the measurement of evapotranspiration

Abstract An experiment was conducted in a 36 ha alfalfa field in Logan, Utah, USA, during three growth-harvest cycles of alfalfa in April–July 1992. A Campbell Scientific (CS) Bowen Ratio System was used to measure continuously 20 min means of micrometeorological elements such as air and dew point temperatures at 1 and 2 m, solar and net radiation, wind speed and direction at 2 m, soil heat fluxes at 8 cm (two locations), and soil temperatures at 2 and 6 cm during the three consecutive growing cycles. The gravimetric method (for 0–10 cm) and the Campbell Pacific Nuclear (CPN) Hydroprobe (for 20–150cm) were used to measure soil moisture daily at 09:00 h. The 24 h evapotranspiration (LE, using the Bowen ratio-energy balance method) was computed by adding the 20 min mean LE values from 09:00 h until 09:00 h of the next day for comparison with the water balance method. Evapotranspiration measured by the water balance method was 98% of that measured by the Bowen ratio-energy balance method during the experimental period (r = 0.987).

Evapotranspiration from the margin and moist playa of a closed desert valley.

Two long term microclimate measurement stations with Bowen ratio capability have been used to study water cycling in a closed desert basin. Microclimate variables including the temperature and vapor pressure gradients were monitored continuously and were used to estimate the Bowen ratio, sensible and latent heat fluxes during 1986 and 1987. Despite having a water table that varied between the surface and 30 cm below the surface, the playa had little evaporation except after rainfall events. The very high osmotic pressure of the soil and salt crust caused most of the absorbed radiation to be partitioned to sensible heat. In contrast, along the margin the thin grass and brush cover transpired water freely, with the latent heat flux exceeding 60% of available energy for much of the season. The higher air temperatures above the playa raised potential evapotranspiration (ET) significantly higher than along the margin throughout the summer. The annual average actual ET of the playa was only 36% of the margin. During the drier summer period (May–October), this ratio decreased to 638 mm were lost from the margin groundwater supply. The 24-h solar and net radiation correlations were 0.80 and 0.94 for the playa and margin, respectively. The lower correlation for the playa resulted from the wide variation of albedo with surface moisture changes. The annual average albedo values for the playa and margin were 0.64 and 0.46, respectively.

Gravity independence of seed-to-seed cycling in Brassica rapa.

Abstract. Growth of higher plants in the microgravity environment of orbital platforms has been problematic. Plants typically developed more slowly in space and often failed at the reproductive phase. Short-duration experiments on the Space Shuttle showed that early stages in the reproductive process could occur normally in microgravity, so we sought a long-duration opportunity to test gravitys role throughout the complete life cycle. During a 122-d opportunity on the Mir space station, full life cycles were completed in microgravity with Brassica rapa L. in a series of three experiments in the Svet greenhouse. Plant material was preserved in space by chemical fixation, freezing, and drying, and then compared to material preserved in the same way during a high-fidelity ground control. At sampling times 13 d after planting, plants on Mir were the same size and had the same number of flower buds as ground control plants. Following hand-pollination of the flowers by the astronaut, siliques formed. In microgravity, siliques ripened basipetally and contained smaller seeds with less than 20% of the cotyledon cells found in the seeds harvested from the ground control. Cytochemical localization of storage reserves in the mature embryos showed that starch was retained in the spaceflight material, whereas protein and lipid were the primary storage reserves in the ground control seeds. While these successful seed-to-seed cycles show that gravity is not absolutely required for any step in the plant life cycle, seed quality in Brassica is compromised by development in microgravity.

Farming in space: environmental and biophysical concerns

The colonization of space will depend on our ability to routinely provide for the metabolic needs (oxygen, water, and food) of a crew with minimal re-supply from Earth. On Earth, these functions are facilitated by the cultivation of plant crops, thus it is important to develop plant-based food production systems to sustain the presence of mankind in space. Farming practices on earth have evolved for thousands of years to meet both the demands of an ever-increasing population and the availability of scarce resources, and now these practices must adapt to accommodate the effects of global warming. Similar challenges are expected when earth-based agricultural practices are adapted for space-based agriculture. A key variable in space is gravity; planets (e.g. Mars, 1/3 g) and moons (e.g. Earths moon, 1/6 g) differ from spacecraft orbiting the Earth (e.g. Space stations) or orbital transfer vehicles that are subject to microgravity. The movement of heat, water vapor, CO2 and O2 between plant surfaces and their environment is also affected by gravity. In microgravity, these processes may also be affected by reduced mass transport and thicker boundary layers around plant organs caused by the absence of buoyancy dependent convective transport. Future space farmers will have to adapt their practices to accommodate microgravity, high and low extremes in ambient temperatures, reduced atmospheric pressures, atmospheres containing high volatile organic carbon contents, and elevated to super-elevated CO2 concentrations. Farming in space must also be carried out within power-, volume-, and mass-limited life support systems and must share resources with manned crews. Improved lighting and sensor technologies will have to be developed and tested for use in space. These developments should also help make crop production in terrestrial controlled environments (plant growth chambers and greenhouses) more efficient and, therefore, make these alternative agricultural systems more economically feasible food production systems.

Analysis of the spaceflight effects on growth and development of Super Dwarf wheat grown on the Space Station Mir.

The hypothesis being tested is that Super Dwarf wheat, Triticum aestivum L., plants in the Svet Greenhouse onboard the Russian Space Station Mir will complete a life cycle in spaceflight, providing that the environmental conditions necessary for adequate growth on Earth are supplied. Twenty six seeds of wheat were planted in each of 2 rows of 2 root compartments for a total of 104 seeds in Svet. Germination rate at 7 d was 56 and 73% on Mir and 75 and 90% in ground-based controls. Plants were grown throughout the whole cycle of ontogenesis (123 d) with samples gathered at different times to validate the morphological and reproductive stages of the plants. Young plants showed vigorous early seedling growth, with large biomass production, including the formation of 280 floral spikes. Upon return to Earth, comparative analyses showed that the number of tillers and flowers per spikelet were 63.2% and 40% greater, respectively, in Mir-grown plants than in the controls. By contrast, the stem length (52.4%), spike mass (49.2%) and length (23.1%), awn length (75.7%), number of spikelets per spike (42.8%) and number of seeds per spike (100% sterile) from Mir-grown plants were substantially less than the controls. Distribution of moisture and roots throughout the substrate was very good. All florets on Mir-grown spikes ceased development at the same stage of ontogeny. Lack of caryopses formation was attributed to male sterility occurring at different stages of staminal development. Anthers failed to dehisce and pollen grains were smaller and shriveled compared to the controls, suggesting a chronic stress had occurred in the Svet growth chamber. Recent ground-based studies indicated that ethylene, which was measured at 0.3 to 1.8 mg kg-1 in the Mir, almost certainly could have induced male sterility in the wheat plants grown on the Mir.

Comparative floral development of Mir-grown and ethylene-treated, earth-grown Super Dwarf wheat

To study plant growth in microgravity, we grew Super Dwarf wheat (Triticum aestivum L.) in the Svet growth chamber onboard the orbiting Russian space station, Mir, and in identical ground control units at the Institute of BioMedical Problems in Moscow, Russia. Seedling emergence was 56% and 73% in the two root-module compartments on Mir and 75% and 90% on earth. Growth was vigorous (produced ca. 1 kg dry mass), and individual plants produced 5 to 8 tillers on Mir compared with 3 to 5 on earth-grown controls. Upon harvest in space and return to earth, however, all inflorescences of the flight-grown plants were sterile. To ascertain if Super Dwarf wheat responded to the 1.1 to 1.7 micromoles mol-1 atmospheric levels of ethylene measured on the Mir prior to and during flowering, plants on earth were exposed to 0, 1, 3, 10, and 20 micromoles mol-1 of ethylene gas and 1200 micromoles mol-1 CO2 from 7 d after emergence to maturity. As in our Mir wheat, plant height, awn length, and the flag leaf were significantly shorter in the ethylene-exposed plants than in controls; inflorescences also exhibited 100% sterility. Scanning-electron-microscopic (SEM) examination of florets from Mir-grown and ethylene-treated, earth-grown plants showed that development ceased prior to anthesis, and the anthers did not dehisce. Laser scanning confocal microscopic (LSCM) examination of pollen grains from Mir and ethylene-treated plants on earth exhibited zero, one, and occasionally two, but rarely three nuclei; pollen produced in the absence of ethylene was always trinucleate, the normal condition. The scarcity of trinucleate pollen, abrupt cessation of floret development prior to anthesis, and excess tillering in wheat plants on Mir and in ethylene-containing atmospheres on earth build a strong case for the ethylene on Mir as the agent for the induced male sterility and other symptoms, rather than microgravity.

Gas Diffusion Measurement and Modeling in Coarse-Textured Porous Media

Conventional gas diffusion measurements in coarse-textured and aggregated porous media are severely limited due to hydrostatically induced variations in water content and air-filled porosity. Motivated by the need to measure gas diffusion in coarse-textured plant growth media designed for use in microgravity (e.g., aboard the International Space Station), our objectives were (i) to develop and test an automated diffusion measurement system on earth with water content adjustment capability and that minimizes hydrostatic effects, and (ii) to model characteristics of gas diffusion in partially saturated aggregated porous media. The horizontally oriented O 2 diffusion cell design for reducing the gravitational effect was based on a thin profile rectangular cell. Continuous measurement of O 2 in sealed dual-chamber diffusion cells provided concentration data for fitting diffusion coefficients where water content was controlled volumetrically using a porous membrane with an imposed pressure for incremental addition or removal of water. Gas diffusion was modeled as a function of air-filled porosity in millimeter-sized aggregated particles exhibiting a substantial difference between internal and external aggregate pore sizes. For this case, the internal aggregate porosity contribution to diffusion compared with external aggregate pore space was minor as described by a dual-porosity diffusion model. The measurement approach described can be used in other coarse-textured and structured porous media.

Aglite lidar: a portable elastic lidar system for investigating aerosol and wind motions at or around agricultural production facilities

The Aglite Lidar is a portable scanning lidar that can be quickly deployed at agricultural and other air quality study sites. The purpose of Aglite is to map the concentration of PM 10 and PM 2.5 in aerosol plumes from agricultural and other sources. Aglite uses a high-repetition rate low-pulse energy 3-wavelength YAG laser with photon-counting detection together with a steerable pointing mirror to measure aerosol concentration with high spatial and temporal resolution. Aglite has been used in field campaigns in Iowa, Utah and California. The instrument is described, and performance and lidar sensitivity data are presented. The value of the lidar in aerosol plume mapping is demonstrated, as is the ability to extract wind-speed information from the lidar data.

Aglite lidar: calibration and retrievals of well characterized aerosols from agricultural operations using a three-wavelength elastic lidar

Lidar (LIght Detection And Ranging) provides the means to quantitatively evaluate the spatial and temporal variability of particulate emissions from agricultural activities. AGLITE is a three-wavelength portable scanning lidar system built at the Space Dynamic Laboratory (SDL) to measure the spatial and temporal distribution of particulate concentrations around an agricultural facility. The retrieval algorithm takes advantage of measurements taken simultaneously at three laser wavelengths (355, 532, and 1064 nm) to extract particulate optical parameters, convert these parameters to volume concentration, and estimate the particulate mass concentration of a particulate plume. The quantitative evaluation of particulate optical and physical properties from the lidar signal is complicated by the complexity of particle composition, particle size distribution, and environmental conditions such as heterogeneity of the ambient air conditions and atmospheric aerosol loading. Additional independent measurements of particulate physical and chemical properties are needed to unambiguously calibrate and validate the particulate physical properties retrieved from the lidar measurements. The calibration procedure utilizes point measurements of the particle size distribution and mass concentration to characterize the aerosol and calculate the aerosol parameters. Once calibrated, the Aglite system is able to map the spatial distribution and temporal variation of the particulate mass concentrations of aerosol fractions such as TSP, PM 10, PM 2.5, and PM 1. This ability is of particular importance in the characterization of agricultural operations being evaluated to minimize emissions and improve efficiency, especially for mobile source activities.

Microgravity effects on water supply and substrate properties in porous matrix root support systems

The control of water content and water movement in granular substrate-based plant root systems in microgravity is a complex problem. Improper water and oxygen delivery to plant roots has delayed studies of the effects of microgravity on plant development and the use of plants in physical and mental life support systems. Our international effort (USA, Russia and Bulgaria) has upgraded the plant growth facilities on the Mir Orbital Station (OS) and used them to study the full life cycle of plants. The Bulgarian-Russian-developed Svet Space Greenhouse (SG) system was upgraded on the Mir OS in 1996. The US developed Gas Exchange Measurement System (GEMS) greatly extends the range of environmental parameters monitored. The Svet-GEMS complex was used to grow a fully developed wheat crop during 1996. The growth rate and development of these plants compared well with earth grown plants indicating that the root zone water and oxygen stresses that have limited plant development in previous long-duration experiments have been overcome. However, management of the root environment during this experiment involved several significant changes in control settings as the relationship between the water delivery system, water status sensors, and the substrate changed during the growth cycles.