Genta Karino

Familiarity perception call elicited under restricted sensory cues in peer-social interactions of the domestic chick.

Social cognitive mechanisms are central to understanding developmental abnormalities, such as autistic spectrum disorder. Peer relations besides parent-infant or pair-bonding interactions are pivotal social relationships that are especially well developed in humans. Cognition of familiarity forms the basis of peer socialization. Domestic chick (Gallus gallus) studies have contributed to our understanding of the developmental process in sensory-motor cognition but many processes remain unknown. In this report, we used chicks, as they are precocial birds, and we could therefore focus on peer interaction without having to consider parenting. The subject chick behavior towards familiar and unfamiliar reference peers was video-recorded, where the subject and the reference were separated by either an opaque or transparent wall. Spectrogram and behavior correlation analyses based on principal component analysis, revealed that chicks elicited an intermediate contact call and a morphologically different distress call, more frequently towards familiar versus unfamiliar chicks in acoustic only conditions. When both visual and acoustic cues were present, subject chicks exhibited approaching and floor pecking behavior, while eliciting joyful (pleasant) calls, irrespective of whether reference peers were familiar or unfamiliar. Our result showed that chicks recognized familiarity using acoustic cues and expressed cognition through modified distress calls. These finding suggests that peer affiliation may be established by acoustic recognition, independent of visual face recognition, and that eventually, both forms of recognition are integrated, with modulation of acoustic recognition.

Peer attachment formation by systemic redox regulation with social training after a sensitive period

Attachment formation is the most pivotal factor for humans and animals in the growth and development of social relationships. However, the developmental processes of attachment formation mediated by sensory-motor, emotional, and cognitive integration remain obscure. Here we developed an animal model to understand the types of social interactions that lead to peer-social attachment formation. We found that the social interaction in a sensitive period was essential to stabilise or overwrite the initially imprinted peer affiliation state and that synchronised behaviour with others based on common motivations could be a driver of peer social attachment formation. Furthermore, feeding experience with supplementation of ubiquinol conferred peer social attachment formation even after the sensitive period. Surprisingly, the experience of feeding beyond the cage window was also effective to reduce the required amount ubiquinol, suggesting that peri-personal space modulation may affect socio-emotional cognition and there by lead to attachment formation.

Socio-emotional development evaluated by Behaviour Output analysis for Quantitative Emotional State Translation (BOUQUET): Towards early diagnosis of individuals with developmental disorders

For citation purposes: Koshiba M, Nakamura S, Mimura K, Senoo A, Karino G, Amemiya S, et al. Socio-emotional development evaluated by Behaviour Output analysis for Quantitative Emotional State Translation (BOUQUET): towards early diagnosis of individuals with developmental disorders. OA Autism 2013 Jul 21;1(2):18. Co m pe tin g in te re st s: n on e de cl ar ed . C on fli ct o f i nt er es ts : n on e de cl ar ed .

A cross-species socio-emotional behaviour development revealed by a multivariate analysis

Recent progress in affective neuroscience and social neurobiology has been propelled by neuro-imaging technology and epigenetic approach in neurobiology of animal behaviour. However, quantitative measurements of socio-emotional development remains lacking, though sensory-motor development has been extensively studied in terms of digitised imaging analysis. Here, we developed a method for socio-emotional behaviour measurement that is based on the video recordings under well-defined social context using animal models with variously social sensory interaction during development. The behaviour features digitized from the video recordings were visualised in a multivariate statistic space using principal component analysis. The clustering of the behaviour parameters suggested the existence of species- and stage-specific as well as cross-species behaviour modules. These modules were used to characterise the behaviour of children with or without autism spectrum disorders (ASDs). We found that socio-emotional behaviour is highly dependent on social context and the cross-species behaviour modules may predict neurobiological basis of ASDs.

Susceptible period of socio-emotional development affected by constant exposure to daylight

As a diurnal experimental primate, the common marmoset (Callithrix jacchus) has recently contributed to numerous kinds of studies of neurobiological psychiatry as an essential pre-clinical model. The marmoset matures sexually within one or two years after birth. Thus, we can observe how the primate learns and develops psycho-cognitive functions through experiences in experimental environment for a much shorter period compared to that of humans. Longer daylight exposure may affect psychological development of children. In our research, we focus on raising marmosets under constant daylight from birth until various ages. In order to quantitatively evaluate the development of higher-ordered psychological functions, we designed a system of socio-behavioral tests and multivariate correlation analysis methods based on principal component analysis. With reference to the call and typical body movement expressed during a particular social context, we statistically inferred the emotional features of the subjects. In the current literature, we review our published results showing increased alert behaviors by constant light, and then, attempted to extend our additional analysis to seek age-dependent susceptibility to constant light. We then present the neurobiological mechanisms with reference to previous research reports. The current review suggests possible existence of a susceptible period earlier than three to five month-old in the environment-induced developmental disorder model, supposedly like attention deficit hyperactive disorders (ADHD) or oppositional defiant disorder (ODD).

Potential of a smartphone as a stress-free sensor of daily human behaviour

Behaviour is one of the most powerful objective signals that connotes psychological functions regulated by neuronal network systems. This study searched for simple behaviours using smartphone sensors with three axes for measuring acceleration, angular speed and direction. We used quantitative analytic methodology of pattern recognition for work contexts, individual workers and seasonal effects in our own longitudinally recorded data. Our 13 laboratory members were involved in the care of common marmosets and domestic chicks, which lived in separate rooms. They attached a smartphone to their front waist-belts during feeding and cleaning in five care tasks. Behavioural characteristics such as speed, acceleration and azimuth, pitch, and roll angles were monitored. Afterwards, participants noted subjective scores of warmth sensation and work efficiency. The multivariate time series behavioral data were characterized by the subjective scores and environmental factors such as room temperature, season, and humidity, using the linear mixed model. In contrast to high-precision but stress-inducing sensors, the mobile sensors measuring daily behaviours allowed us to quantify the effects of the psychological states and environmental factors on the behavioural traits.

The social interaction effect on circadian rhythm of body temperature and locomotion activity captured through longitudinal monitoring by thermo-imagery sensor

Neurons in the preoptic area (POA), especially the ventral lateral preoptic area and the median preoptic nucleus, fire rapidly during sleep and cease firing during wakefulness. These neurons carry GABA, and thought to play an important role in initiation and maintenance of sleep by sending inhibitory projections to the arousal systems that reside in the brain stem. Recently, several evidence have suggested that orexinergic neurons in the hypothalamus, which play a critical role in maintaining arousal, are also influenced by these neurons. To elucidate the roles of these neurons in regulation of orexin neurons, we optogenetically stimulate these sleep-active neurons. We used Gad1-Cre knock-in mice, in which Cre recombinase is exclusively expressed in GABAergic neurons. We used an adeno-associated viral vector to deliver channelrhodopsin-2-YFP to Cre-expressing neurons in the POA. The axonal projection of the GABAegic neurons of the POA was visualized with double-label immunohistochemistry used anti orexin antiserum combined with an anti-GFP antiserum. Rich immunoreactivities of GFP-ir projections were observed in arousal region including the LHA. Optogenetic stimulation of POA GABAergic neurons resulted in increase of NREM sleep accompanied by inhibition of orexin neurons. These observations suggest that the POA GABAergic neurons are important in inhibition of arousal regions including hypothalamic orexin neurons.