Gerhard Baumann

A highly crosslinked, transparent polyacrylamide gel with improved mechanical stability for use in isoelectric focusing and isotachophoresis

Polyacrylamide gels highly (10–50%) crosslinked with N,N′-methylenebisacrylamide (BIS) provide large pore sizes but are mechanically labile. By substituting N,N′-diallyltartardiamide (DATD) for BIS, mechanical stability and firm adherence to glass walls is conferred to these gels, with only a minor decrease in pore size compared to highly BIS-crosslinked gels. The highly DATD-crosslinked gels have the further advantage of being transparent. It appears that highly DATD-crosslinked gels combining large pore size with mechanical stability are superior to presently used gel types in isoelectric focusing on polyacrylamide gel and isotachophoresis on polyacrylamide gel.

Lack of interaction between hormonal proteins and synthetic carrier ampholytes (Ampholine)

Abstract Using 14 C-labeled Ampholine, radioiodinated and unlabeled human growth hormone, and ovine prolactin, no significant degree of interaction could be detected by polyacrylamide gel electrophoresis and gel chromatography. Protein binding to excess Ampholine was negligible. Ampholine binding to excess protein was below the detection limit of 0.2 moles of Ampholine per mole of protein. Commercial Ampholine contains large molecular weight components that cannot be separated by gel chromatography from proteins in the 20–30,000-molecular weight range.

A simple electronic device for the measurement of band migration distances in polyacrylamide gels

Abstract An electronic device for the measurement of migration distances in polyacrylamide gel electrophrresis was developed and tested. Principal advantages over manual methods are: Speed, ease of operation, improved detection of minor bands, and adaptability to automated data storage and processing.