Gi Dae Bok

PALEOPARASITOLOGICAL REPORT ON THE STOOL FROM A MEDIEVAL CHILD MUMMY IN YANGJU, KOREA

Previous studies have successfully shown evidence for parasitic infections in human remains from various archaeological sites. However, in the case of Korea, since there have been very few paleoparasitological reports published, pre-20th century parasitic infection patterns remain obscure. Therefore, in order to partly fill this gap, we are reporting on a case of paleoparasitic infection from the feces of a 15th century child mummy from Yangju, Korea. In the course of the present study, we found the eggs of Clonorchis sinensis, Ascaris lumbricoides, and Trichuris trichiura in the feces of the mummy. Trichuris trichiura eggs were found in far greater numbers than other parasite eggs; in fact, intact bipolar plugs were clearly observed and even the larvae were still visible in some eggs. The eggs of C. sinensis and A. lumbricoides were also well preserved, though not in as great a number. Since we could find a number of well-preserved larvae-containing eggs, we are encouraged that successful extraction, amplification, and sequence determination of ancient DNA from the paleoparasite eggs might be possible in future studies. With additional paleoparasitological investigation using feces from Korean mummies, we hope that a history of parasite infection in Korea will be reconstructed.

SCANNING ELECTRON MICROSCOPE STUDY OF ANCIENT PARASITE EGGS RECOVERED FROM KOREAN MUMMIES OF THE JOSEON DYNASTY

Abstract We have previously shown that parasite eggs have been identified in the coprolites of Korean mummies. These eggs have shed light on parasitic infection patterns in Korean populations living several hundred years ago. We conducted a scanning electron microscopy (SEM) study on ancient Trichuris trichiura, Ascaris lumbricoides, Metagonimus yokogawai, Paragonimus westermani, and Gymnophalloides seoi eggs recovered from Korean mummies of the Joseon Dynasty. We anticipated that the taphonomic conditions of mummification would alter the eggs of certain species but not of others. Our SEM data show that each species of ancient egg exhibited different degrees of preservation. Thus, some of them, for example, M. yokogawai, exhibited a better preservation status than others, suggesting that they should be the first candidates considered when choosing subjects for future paleoparasitological studies.

Endoscopic investigation of the internal organs of a 15th-century child mummy from Yangju, Korea.

Our previous reports on medieval mummies in Korea have provided information on their preservation status. Because invasive techniques cannot easily be applied when investigating such mummies, the need for non‐invasive techniques incurring minimal damage has increased among researchers. Therefore, we wished to confirm whether endoscopy, which has been used in non‐invasive and minimally invasive studies of mummies around the world, is an effective tool for study of Korean mummies as well. In conducting an endoscopic investigation on a 15th‐century child mummy, we found that well‐preserved internal organs remained within the thoracic, abdominal and cranial cavities. The internal organs – including the brain, spinal cord, lung, muscles, liver, heart, intestine, diaphragm and mesentery – were easily investigated by endoscopy. Even the stool of the mummy, which accidentally leaked into the abdominal cavity during an endoscopic biopsy, was clearly observed. In addition, unusual nodules were found on the surface of the intestines and liver. Our current study therefore showed that endoscopic observation could provide an invaluable tool for the palaeo‐pathological study of Korean mummies. This technique will continue to be used in the study of medieval mummy cases in the future.

Magnetic resonance imaging performed on a hydrated mummy of medieval Korea

Previous investigations have shown that magnetic resonance imaging (MRI) can be employed as an efficient non‐invasive diagnostic tool in studies on Egyptian mummies. MRI, moreover, because it produces especially clear images of well‐hydrated tissue, could be a particularly effective diagnostic option for mummies that still retain humidity within tissues or organs. Therefore, in the present study, we tested MRI on a 17th century mummy, one of the most perfectly preserved ‘hydrated mummies’ ever found in Korea, in order to determine the quality of images that could be obtained. We found that the diagnostic value of an MRI scan of the hydrated mummy was not inferior to that of a computed tomography scan. The T1‐ and T2‐weighted magnetic resonance (MR) signals showed unique patterns not easily obtained by computed tomography, the resultant MR images revealing the organ specificities clearly. Overall, the quality of the MR images from the hydrated mummy was superb and the scientific value of MRI in the study of hydrated mummies should not be underestimated.

Acute traumatic death of a 17th century general based on examination of mummified remains found in Korea

Recently, we examined one of the most perfectly preserved mummies of the Joseon Dynasty (1392-1910) ever found in Korea. The individual was an elderly man and a high-ranking general who had lived sometime during the 16th or 17th century in Korea. When computerized tomography (CT) radiographs were taken, a fracture line was observed on the left side of the mandible. A post-factum dissection also provided crucial clues to the cause of death. First of all, blood clots were still evident at the fracture site, indicating that the mandibular fracture had occurred just before death. Second, we also found feces exclusively in the sigmoid colon or rectum, but not in the stomach, small intestine or colon. This told us that our subject had not eaten anything during his final 2 days (even though there was no indication that he would have had any difficulty eating during that time). Therefore, we presume that this case might not be one of chronic or wasting disease, but rather a case of sudden death. By virtue of the varied specialties of the researchers involved in this study, we were able to piece together a partly very clear and partly very plausible story for our 17th century mummy subject. Considering the high level of preservation of remains and artifacts found in lime soil mixture barrier (LSMB) tombs, not to mention the rich supplementary information available from historical documents, similarly successful studies are promised in forthcoming days and years.

The potential for non-invasive study of mummies: validation of the use of computerized tomography by post factum dissection and histological examination of a 17th century female Korean mummy.

The socio‐cultural antipathies of some descendants with regard to invasive examinations of age‐old human remains make permission for dissection of Korean mummies of the Joseon Dynasty (1392–1910) difficult to obtain. Overcoming this obstacle necessitated the use of non‐invasive techniques, such as multi‐detector computerized tomography (MDCT) and endoscopic examination, enabling determination of the preservation status of internal organs of mummies without significantly damaging the mummies themselves. However, MDCT alone cannot clearly differentiate specific mummified organs. Therefore, in much the same way as diagnostic radiologists make their MDCT readings on living patients more reliable by means of comparison with accumulated post‐factum data from autopsies or histological studies, examinations of mummies by invasive techniques should not be decried as mere destruction of age‐old human remains. Rather, providing that due permission from descendants and/or other relevant authorities can be obtained, dissection and histological examination should be performed whenever opportunities arise. Therefore, in this study, we compared the radiological data acquired from a 17th century mummy with our dissection results for the same subject. As accumulation of this kind of data could be very crucial for correct interpretation of MDCT findings on Korean mummies, we will perform similar trials on other Korean mummies found in forthcoming days if conditions permit.