Giordano Cabral Gianotti

Eletroestimulação na cicatrização de feridas cutâneas experimentais em coelhos

Chronic skin wounds are frequently found in veterinary medicine routine and it becomes a challenge for clinicians. Sometimes the treatment routinely used end up failing. The electrotherapy has been indicated as an alternative to conventional treatments because of its ability to promote healing. The aim of this paper is to report an experimental study using electrical stimulation (ES) in wound healing in rabbits. It was evaluated 10 healthy New Zealand rabbits that were submitted to general anesthesia for resection of two wounds with 1cm2 located in both sides of the back behind the scapula edge. It was determinate that the right wound would be treated by ES and the left would be used as a control, both cleaned with solution of NaCl 0,9%. The frequency used to ES was 60Hz for 10 minutes every two days, being applied by acupuncture needles in four equidistant points around the lesion, to 0.5cm from the edge. The animals were divided in one group of 15 days and another one of 8 days for treatment and macroscopic and histopathological evaluation. The results showed that the lesions treated by ES do not have significant difference in healing time. However, those treated had higher scarring macroscopic and microscopic quality than the control group. Therefore, it is possible to indicate the use of ES as a skin wounds treatment, whereas it was demonstrated to be an effective technique, easy to use and has relatively low cost.

Functional and regenerative effects of local administration of autologous mononuclear bone marrow cells combined with silicone conduit on transected femoral nerve of rabbits

The inoculation of cells into injury sites can accelerate and improve the quality of nerve regeneration. This study aimed to evaluate the functional and regenerative effects of mononuclear autologous bone marrow cells (MABMC) combined with silicon conduit grafting in rabbit femoral nerves. Twenty-eight animals were allocated to one of two groups: treatment group (TG) or control group (CG), divided according to the time of evaluation, at either 50 or 75 days. After neurotmesis of the femoral nerve, surgical repair was performed with nerve autografts in silicon conduits, leaving a 5mm gap in both groups. The TG received MABMC in silicon conduits, and CG received a sham saline inoculum. Histological, clinical and electrophysiological analyses detected no differences between groups, but analysis of leg diameter showed that TG diameters were larger. This cell therapy did not improve regeneration of the femoral nerve, but there was a tendency for better functional recovery.

Osteossíntese de tíbia com fixador esquelético externo em um cordeiro

Entre os avancos que a clinica e cirurgia veterinaria apresentaram nos ultimos anos, o tratamento de fraturas em grandes animais pode ser citado como um exemplo. A experiencia, consolidada em outras especies de menor porte, permite a tentativa de extrapolar a mesma para as especies de maior porte, adequando-se sempre as caracteristicas e particularidades de cada especie. Atualmente, devemos considerar o tratamento de fraturas de animais de producao como uma forma de evitar o seu descarte, uma vez que, quando se trata de um animal reprodutor, devemos ter a opcao de trata-lo ao inves de decidir pela eutanasia. O presente estudo relata um caso de fratura transversa de diafise de tibia de membro posterior esquerdo em um cordeiro sem raca definida, com duas semanas de idade, com historico de pisoteamento por um bovino. Foi utilizada fixacao esqueletica externa tipo II para a osteossintese, obtendo-se eficiente estabilizacao da fratura. O animal ficou hospitalizado durante todo o periodo de pos-operatorio e a consolidacao ossea foi obtida em 30 dias.

Células-tronco mesenquimais aplicadas nas fases inflamatória e proliferativa da cicatrização de feridas cutâneas

Wound healing is a process that requires the interaction of various cells in the dermis and epidermis. The aim of this study was to evaluate the action of ADSCs in the treatment of acute wounds in order to understand if application time of the cells results in a difference in healing the first seven days of injury. The stem cells were isolated from adipose tissue of C57BL / 6 mice GFP +. Thus, we used 49 mice C57BL / 6 divided into four groups: Group I (GI / control, n=14); Group II (GII; n=14): ADSCs injected to the d0; Group III (GIII; n=14): ADSCs injected on the 3rd day, and Group IV (GIV; n=7): ADSCs injected day 5(d5). Clinical evaluations were performed on days 0, 3, 5 and 7 and the histopathology on days 5 and 7. In the proposed methodology, the use of ADSCs increased vascularization, formation of granulation tissue, collagen deposition and increases the number of hair follicles in just seven days of evaluation. In addition, the time of application of the cells did not affect significant differences in the inflammatory and the proliferative phase of wound healing skin.

Mielomalácia hemorrágica progressiva em cão

Mielomalacia e a necrose isquemica ou hemorragica da medula espinhal, que pode ocorrer como sequela de uma lesao ocasionada por um trauma espinhal agudo. A mielomalacia pode ser focal ou difusa, sendo a lesao caracterizada por uma combinacao de infarto isquemico e hemorragico do parenquima do cordao espinhal, que migra tanto de forma ascendente como descendente, causando lesoes neurologicas irreversiveis e compativeis com a regiao afetada. Um canino da raca dachshund, cinco anos e 6,7kg foi atendido no Hospital de Clinicas Veterinarias (HCV) da Universidade Federal do Rio Grande do Sul (UFRGS). No exame neurologico apresentou paralisia de membros posteriores, com ausencia de dor profunda e reflexo patelar aumentado. Foi in iciada terapeutica com prednisolona e repouso absoluto, sendo recomendada a reavaliacao em dois dias. No retorno, o canino apresentou tetraparalisia, ausencia de dor profunda nos membros anteriores e posteriores, dispneia e hipotermia. Apos exame radiografico e com base nos sinais clinicos do animal, suspeitou-se de mielomalacia hemorragica progressiva, que foi confirmado no exame de necropsia.