Emerson Antonio Contesini

Ovário-histerectomia laparoscópica em felinos hígidos: estudo comparativo de três métodos de hemostasia

Twenty-four healthy female mongrel cats were submitted to ovaryhisterectomy and distributed into three groups of eight animals each: (I) bipolar electrical cautery, (II) titanium clips, and (III) suture ligature for the occlusion of ovarian and uterine vessels, which were compared. The surgical procedure and the operation technique were viable in all three groups. Hemorrhagia and subcutaneous emphysema were the main intraoperative complications, and hematoma and suture dehiscence in the postoperative. The bipolar electrical cautery procedure is shown to be comparatively successful regarding the other studied methods for hemostasia.

Laparoscopia nas hérnias diafragmáticas: estudo experimental em cães

The most common type of diaphragmatic hernia in dogs and cats is the traumatic one. As an anatomic alteration, surgery is the elected treatment. The purpose of this study was to evaluate videolaparoscopic surgery in the diagnostic and treatment of diaphragmatic hernia in dogs. In the first phase of the study diaphragmatic hernias were performed in dogs. One week later the dogs were summited to a laparoscopic surgery to make diagnostic and surgical correction. Laparoscopic access showed to be efficient for identification of the defect and visceral displacement and for the surgical treatment.The most common type of diaphragmatic hernia in dogs and cats is the traumatic one.As an anatomic alteration surgery is the elected treatment. The purpose of this study was to evaluate videothoracoscopic surgery in the diagnostic and treatment of diaphragmatic hernia in eight dogs. In the first phase of the study diaphragmatic hernias were performed in the dogs through laparoscopic access. One week later the dogs were summited to a thoracoscopic surgery to make diagnostic and surgical correction. Thoracoscopic access showed to be efficient for identification of the defects and visceral displacements and for the surgical treatment.

Utilização de células-tronco autólogas de medula óssea na regeneração do nervo tibial de coelhos mediante técnica de tubulização com prótese de silicone

This study presents an experimental model of an acute deffect in a peripheral nerve to evaluate neural regeneration using a tubulization technique associated with the inoculation of autologous stem cells from bone marrow. A total of 12 New Zealand white rabbits underwent a bilateral dissection of the tibial nerve followed by repair with silicone tubulization. On the left tibial nerve of all animals, the tube was filled with autologous bone marrow-derived stem cells collected from the humerus. For control, using the same repair technique, the tubes were filled with a NaCl solution in the right tibial nerve. After 30 days of observation, the animals were euthanized and a histological evaluation of the collected nerve segments was performed by staining with hematoxylin-eosin, luxol fast blue, and toluidine blue. From the results it is possible to conclude that the transplanted autologous stem cells associated with the tubulization technique present an advantage in the peripheral nerve regeneration process.

Solução hipersaturada de sal ou de glicerina a 98% como conservantes de centros frênicos caninos utilizados na reparação de defeitos musculares em ratos wistar

Aiming to evaluate the over saturated salt solution (1.5g commercially available salt in 1.5ml of tridestilled water) as tissue conservator of the canine frenic center and to compare its conservator potential with the well-established glycerin 98% conservator solution, peaces of this tissue were implanted to repair produced muscle lesions in 28 Wistar rats. Previously both solutions had been tested for bacterial and fungal contaminations, both exams with negative results. In each experimental animal, lesions were made in both Rectus abdominis muscles and repaired with the tissue preserved in both solutions. The animals were divided in seven groups and sacrificed in three, five, seven, 10, 15, 30 and 60 days after reparation for histological evaluation of the implanted region. In macroscopic exam the complete substitution of the implants by live tissue was observed, without rejection of the implanted tissue. In the microscopic evaluation we observed that the substitutions were made by fibrous conjunctive tissue deposition. At the end of the evaluation period, the implanted membranes were not present in repaired regions. In both conserved tissues there were not differences in tissue reactions. The over saturated salt, and 98% glycerin solutions presented similar results in the canine frenic center preservation indicating that both solutions may be utilized to preserve this membrane.

Resistência à tração de colopexias incisionais realizadas por cirurgia laparoscópica ou celiotomia em cães

In the present study the traction resistance and histological features of incisional colopexys made by open or laparoscopic surgery were evaluated. For this purpose, 15 mongrel dogs were located in two groups. In the GL group (n= 8) the colopexy adhesion was made by a laparoscopic surgery, and in GA group (n= 7) by a celiotomy. For the laparoscopic procedures the dogs were positioned in dorsal decubitus and four trocars were introduced in the left and right position of umbilical region. The incisions produced in descendent colon and in the abdominal wall were sutured in two layers of intracorporeal suturing. In the GA group the colon suture was similar to the realized in GL group. Fourteen days after the surgeries the dogs were killed to collect samples of abdominal wall and colon descendent, which were used in resistance tests and in histological evaluations. The total surgery time and colon fixation time were higher in the GL group (p 0.05), and the rupture force in GL segments was 43.68±16.45N and in GA was 39.70±15.15N. No differences were found in histological examination between GL and GA groups, and in all dogs the fibers of collagen were mature. The results verified for the laparoscopic technique of colopexy, that presented surgical adhesions with similar quality of the provoked by a conventional technique, allow concluding that the laparoscopic procedure may be utilized as an alternative for rectal prolapse treatment in dogs.

Treatment of dilated cardiomyopathy in rabbits with mesenchymal stem cell transplantation and platelet-rich plasma

Dilated cardiomyopathy (DCM) is a major cause of cardiovascular mortality and morbidity, and there is evidence to suggest that stem cell transplantation may be a viable treatment option for this condition. Therefore, the goal of the present study was to assess myocardial regeneration in rabbits with doxorubicin-induced DCM treated with adipose mesenchymal stem cells (MSC) alone or in combination with platelet-rich plasma (PRP). Twenty New Zealand rabbits received doxorubicin for the induction of DCM and were divided into four groups according to treatment: saline, MSC, PRP and MSCu2009+u2009RP. Treatment agents were injected directly into the left ventricular myocardium following a thoracoscopy. Rabbits were assessed through echocardiographic and electrocardiographic examinations, as well as serum cardiac troponin I measurements at baseline, after the induction of DCM and 15 days after treatment. Animals were euthanased following the last assessment, and hearts were collected for histopathological analyses. The MSC group showed improvements in all parameters assessed, while the PRP group showed significantly impaired heart function. Histopathology of the heart revealed that the MSC group displayed the lowest number of lesions, while rabbits in the MSCu2009+u2009PRP, saline and PRP groups had steadily advancing lesions. These results suggest that MSC transplantation can improve heart function in rabbits with DCM, and underscore the need for further studies of the effects of PRP on the myocardium.

Meloxicam subaracnoide não inibe a hipernocicepção mecânica no teste da carragenina em ratos

BACKGROUND AND OBJECTIVEnEvaluate the antinociceptive effects of subarachnoid meloxicam on the mechanical hypernociception induced by carrageenan in rats.nnnMETHODSnRandomized controlled trial. Eighteen adult male Wistar rats underwent a cannula implantation into the subarachnoid space and were randomly divided into two groups: Group I (GI) received saline solution 5μL, while Group II (GII) received meloxicam 30mg. The mechanical hypernociception was induced by intraplantar injection of carrageenan and evaluated using a digital analgesymeter every 30minutes during a 4 hour period. The results were recorded as the Δ withdrawal threshold (in g), calculated by subtracting the measurement value afternnnRESULTSnThe Δ withdrawal threshold mean values were lower in the group of patients treated with meloxicam over all time points between 45 and 165minutes, however, there was no statistical significance, (p=0.835) for this difference.nnnCONCLUSIONnSubarachnoid meloxicam at a dose of 30μg.animal(-1) did not suppress the mechanical hypernociception in a model of inflammatory pain induced by intraplantar administration of carrageenan in rats. The data suggest that other dosages should be investigated the drug effect is discarded.

Eletroestimulação na cicatrização de feridas cutâneas experimentais em coelhos

Chronic skin wounds are frequently found in veterinary medicine routine and it becomes a challenge for clinicians. Sometimes the treatment routinely used end up failing. The electrotherapy has been indicated as an alternative to conventional treatments because of its ability to promote healing. The aim of this paper is to report an experimental study using electrical stimulation (ES) in wound healing in rabbits. It was evaluated 10 healthy New Zealand rabbits that were submitted to general anesthesia for resection of two wounds with 1cm2 located in both sides of the back behind the scapula edge. It was determinate that the right wound would be treated by ES and the left would be used as a control, both cleaned with solution of NaCl 0,9%. The frequency used to ES was 60Hz for 10 minutes every two days, being applied by acupuncture needles in four equidistant points around the lesion, to 0.5cm from the edge. The animals were divided in one group of 15 days and another one of 8 days for treatment and macroscopic and histopathological evaluation. The results showed that the lesions treated by ES do not have significant difference in healing time. However, those treated had higher scarring macroscopic and microscopic quality than the control group. Therefore, it is possible to indicate the use of ES as a skin wounds treatment, whereas it was demonstrated to be an effective technique, easy to use and has relatively low cost.

Urethral duplication in a dog: case report

A male Brazilian Fila dog was referred because of a perineal orifice with urine stream during micturition. To determine the extent of the accessory channel, a contrasted retrograde urethrocystography was performed. A surgical correction was undertaken. Histological study showed the presence of the urethral channel lined by multilayer transitional epithelium. The patient recovered well and there was no evidence of complications one year after surgery.

Functional and regenerative effects of local administration of autologous mononuclear bone marrow cells combined with silicone conduit on transected femoral nerve of rabbits

The inoculation of cells into injury sites can accelerate and improve the quality of nerve regeneration. This study aimed to evaluate the functional and regenerative effects of mononuclear autologous bone marrow cells (MABMC) combined with silicon conduit grafting in rabbit femoral nerves. Twenty-eight animals were allocated to one of two groups: treatment group (TG) or control group (CG), divided according to the time of evaluation, at either 50 or 75 days. After neurotmesis of the femoral nerve, surgical repair was performed with nerve autografts in silicon conduits, leaving a 5mm gap in both groups. The TG received MABMC in silicon conduits, and CG received a sham saline inoculum. Histological, clinical and electrophysiological analyses detected no differences between groups, but analysis of leg diameter showed that TG diameters were larger. This cell therapy did not improve regeneration of the femoral nerve, but there was a tendency for better functional recovery.