Giorgio Bertanza

The influence of different disinfectants on mutagenicity and toxicity of urban wastewater

Many human pathogenic micro-organisms can be transmitted by waters contaminated by wastewater effluents. Wastewater disinfection helps prevent the spread of these pathogens in aquatic environments. Chlorination is the most widely used method for the disinfection of wastewater, but can cause the formation of mutagenic/carcinogenic and toxic by-products which are potentially harmful to human and aquatic organisms. The aim of this research was to study the influence of disinfectants alternative to chlorine, such as chlorine dioxide, ozone, peracetic acid and UV radiation, on the formation of mutagenic and toxic compounds in wastewater. Wastewater samples were collected before and after disinfection in summer and in winter and then adsorbed using silica C18 cartridges. Concentrates were tested for mutagenicity using the Ames test. For toxicity test, a bioluminescence assay using Vibrio fischeri photobacteria was employed. To detect DNA damage activity non-concentrated water samples were tested using two genotoxicity plant tests (Allium test and Tradescantia/micronuclei test). All disinfectant treatments produced bacterial mutagenicity, particularly after treatment with ClO2 or ozone. The Allium cepa test gave positive results only for PAA-treated wastewater sampled in winter and the Tradescantia/MCN test always gave negative results. Raw wastewater, ClO2- and PAA-disinfected waters showed toxic effects in the marine bacteria test.

Effect of biological and chemical oxidation on the removal of estrogenic compounds (NP and BPA) from wastewater: An integrated assessment procedure

A major source of the wide presence of EDCs (Endocrine Disrupting Compounds) in water bodies is represented by direct/indirect discharge of sewage. Recent scientific literature reports data about their trace concentration in water, sediments and aquatic organisms, as well as removal efficiencies of different wastewater treatment schemes. Despite the availability of a huge amount of data, some doubts still persist due to the difficulty in evaluating synergistic effects of trace pollutants in complex matrices. In this paper, an integrated assessment procedure was used, based on chemical and biological analyses, in order to compare the performance of two full scale biological wastewater treatment plants (either equipped with conventional settling tanks or with an ultrafiltration membrane unit) and tertiary ozonation (pilot scale). Nonylphenol and bisphenol A were chosen as model EDCs, together with the parent compounds mono- and di-ethoxylated nonylphenol (quantified by means of GC-MS). Water estrogenic activity was evaluated by applying the human breast cancer MCF-7 based reporter gene assay. Process parameters (e.g., sludge age, temperature) and conventional pollutants (e.g., COD, suspended solids) were also measured during monitoring campaigns. Conventional activated sludge achieved satisfactory removal of both analytes and estrogenicity. A further reduction of biological activity was exerted by MBR (Membrane Biological Reactor) as well as ozonation; the latter contributed also to decrease EDC concentrations.

A Comparison Among Different Wastewater Disinfection Systems: Experimental Results

In this work, the efficiency and the applicability of some disinfection systems for biologically treated wastewater were studied. The following disinfection processes were considered: peracetic acid (PAA), chlorine dioxide, ozone, UV light. These treatments were chosen as an alternative to chlorination. Their applicability was investigated considering both the removal efficiency of microorganisms and the qualitative changes in the treated effluent (e.g. presence of disinfectant residues, disinfection by-products, etc.). The different processes were tested by means of pilot scale plants working in parallel; in fact they were fed with the same wastewater (secondary effluent of a municipal wastewater treatment plant). Finally, an economical analysis was carried out.

EDCs, estrogenicity and genotoxicity reduction in a mixed (domestic + textile) secondary effluent by means of ozonation: A full-scale experience

WWTP (wastewater treatment plant) effluents are considered to be a major source for the release in the aquatic environment of EDCs (Endocrine-Disrupting Compounds), a group of anthropogenic substances able to alter the normal function of the endocrine system. The application of conventional processes (e.g. activated sludge with biological nitrogen removal) does not provide complete elimination of all these micropollutants and, consequently, an advanced treatment should be implemented. This experimental work was conducted on the tertiary ozonation stage of a 140,000 p.e. activated sludge WWTP, treating a mixed domestic and textile wastewater: an integrated monitoring, including both chemical (nonylphenol, together with the parent compounds mono- and di-ethoxylated, and bisphenol A were chosen as model EDCs) and biological (estrogenic and genotoxic activities) analyses, was carried out. Removal efficiencies of measured EDCs varied from 20% to 70%, depending on flow conditions (ozone dosage being 0.5 gO3/gTOC). Biological tests, furthermore, displayed that the oxidation stage did not significantly reduce (only by 20%) the estrogenicity of the effluent and revealed the presence and/or formation of genotoxic compounds. These results highlight the importance of the application of an integrated (biological+chemical) analytical procedure for a global evaluation of treatment suitability; poor performances recorded in this study have been attributed to the presence of a significant industrial component in the influent wastewater.

Automatic evaluation of design alternatives with quantitative argumentation

This paper presents a novel argumentation framework to support Issue-Based Information System style debates on design alternatives, by providing an automatic quantitative evaluation of the positions put forward. It also identifies several formal properties of the proposed quantitative argumentation framework and compares it with existing non-numerical abstract argumentation formalisms. Finally, the paper describes the integration of the proposed approach within the design Visual Understanding Environment software tool along with three case studies in engineering design. The case studies show the potential for a competitive advantage of the proposed approach with respect to state-of-the-art engineering design methods.

Biodegradability, toxicity and mutagenicity of detergents: Integrated experimental evaluations.

The widespread use of detergents has raised concern with regard to the environmental pollution caused by their active ingredients, which are biorefractory, toxic and persistent. Since detergents are complex mixtures of different substances, in which synergistic effects may occur, we aimed to assess the mutagenicity of different detergent formulations, taking into account aquatic toxicity and ready biodegradability. We performed a ready biodegradability test (OECD 301 F), Daphnia magna and Vibrio fischeri toxicity tests, and mutagenicity tests (Salmonella/microsome test, Allium cepa test and comet assay). Six detergent formulations were examined, 3 pre-manufacture and 3 commercially available. All detergents presented ready biodegradability. EC50 values varied for all products, according to the marker organism used, but were always higher than the more stringent value considered for aquatic toxicity assessment (V. fischeri 10-60 mg/L; D. magna 25-300 mg/L; A. cepa 250-2000 mg/L). None of the detergents caused mutations in bacteria. However, one commercial ecolabelled product induced an increase in micronucleus frequency in A. cepa root cells. All pre-manufacture detergents and one commercial one, which gave negative results in the Ames and A. cepa tests, induced DNA damage in human leukocytes. A more accurate evaluation of the environmental impact of complex mixtures such as detergents requires a battery of tests to describe degradation, as well as toxicological and mutagenic features.

Techno-economic and environmental assessment of sewage sludge wet oxidation.

Today, several technologies and management strategies are proposed and applied in wastewater treatment plants (WWTPs) to minimise sludge production and contamination. In order to avoid a shifting of burdens between different areas, their techno-economic and environmental performance has to be carefully evaluated. Wet oxidation (WO) is an alternative solution to incineration for recovering energy in sewage sludge while converting it to mostly inorganic residues. This paper deals with an experimentation carried out within the EU project “ROUTES”. A mass balance was made for a WWTP (500,000 person equivalents) in which a WO stage for sludge minimisation was considered to be installed. Both bench- and full-scale test results were used. Design of treatment units and estimation of capital and operational costs were then performed. Subsequently, technical and economic aspects were evaluated by means of a detailed methodology which was developed within the ROUTES project. Finally, an assessment of environmental impacts from a life cycle perspective was performed. The integrated assessment showed that for the specific upgrade considered in this study, WO technology, although requiring a certain increase of technical complexity at the WWTP, may contribute to environmental and economic advantages. The paper provides guidance in terms of which aspects need a more thorough evaluation in relation to the specific case in which an upgrade with WO is considered.

Ammonia stripping from MSW landfill leachate in bubble reactors: process modeling and optimization

This work presents a study of the ammonia air stripping pro cess in a semi-flow batch bubble reactor as a pre-treatment for municipal solid waste (MSW) landfill leachate. In order to assess the optimum treatment conditions from a technical and an economic point of view, the research was carried out in the following steps: 1) experimentation in a pilot plant; 2) mathematical model development; and 3) economic analysis. The experimentation showed that it is possible to achieve high ammonia removal efficiencies even without basificant dosage, if temperature is maintained between approximately 60 and 70°C. The development and calibration of a mathe matical model permitted the study to determine the treat ment efficiency of the process under several operating condi tions. The economic analysis has shown that, with a removal efficiency of approximately 70%, it is convenient to work without basificant dosage and at a temperature of 70°C. These conditions can also be considered optimal from a tech mcal point of view.

Integration between chemical oxidation and membrane thermophilic biological process

Full scale applications of activated sludge thermophilic aerobic process for treatment of liquid wastes are rare. This experimental work was carried out at a facility, where a thermophilic reactor (1,000 m(3) volume) is operated. In order to improve the global performance of the plant, it was decided to upgrade it, by means of two membrane filtration units (ultrafiltration -UF-, in place of the final sedimentation, and nanofiltration -NF-). Subsequently, the integration with chemical oxidation (O(3) and H(2)O(2)/UV processes) was taken into consideration. Studied solutions dealt with oxidation of both the NF effluents (permeate and concentrate). Based on experimental results and economic evaluation, an algorithm was proposed for defining limits of convenience of this process.

How green are environmental technologies? A new approach for a global evaluation: The case of WWTP effluents ozonation

The research on the impact of chemical pollution is now increasingly attracted by the topic of organic micropollutants: as secondary biological treatment of wastewater does not provide the complete elimination of these substances, an advanced treatment downstream the biological process can be implemented. Notwithstanding, the benefits of improved effluent quality can be weakened by the negative effects on air quality, when energy consumption and related pollutants emissions deriving from the advanced treatment technologies are taken into account. It is the aim of this work to present an innovative methodology to judge the environmental compatibility of wastewater treatment processes on the basis of the damage on human health produced/avoided, expressed as an economic value. In particular, while for air pollution the established external costs were applied, for water pollution the rates of the impacts on human health have been evaluated in terms of Global Burden of Disease and measured in units of DALY (Disability-Adjusted Life Years), then converted into costs based on Gross Domestic Product. As a first application, this procedure was used for assessing environmental compatibility of a final ozonation: the results of this study showed that the reduction of water pollution achieved by means of ozonation might be beneficial for human health at an extent which is in the same order of magnitude of damage caused by air pollution, emphasizing that the question if the use of advanced (energy-intensive) treatments is a proper solution to remove organic micropollutants from wastewater remains still open.