Gisella Paci

Effect of an outdoor rearing system on the welfare, growth performance, carcass and meat quality of a slow-growing rabbit population

The effect of Outdoor or Indoor housing systems on the growth, welfare and carcass and meat quality of a local rabbit population was investigated. The slaughter age was 103±2days. Open-field tests showed an effective capacity of the Outdoor group to combat stressors. Compared to Indoor rabbits, Outdoor rabbits showed better growth performance and higher slaughter weight (SW) (2535 vs 2137g; P<0.01). Outdoor housing conditions increased the physical activity of rabbits and their hind legs were more developed (36.1% vs 34.9%; P<0.01). Slaughter yield was lower in Outdoor rabbits (57.8% vs 58.4% SW; P<0.05) due to the higher skin proportion (17.2% vs 15.6% SW; P<0.05). Outdoor rabbit meat showed lower L∗ value (L. lumborum: 55.6 vs 59.2; P<0.01; B. femoris: 53.0 vs 55.5; P<0.01) and cooking loss (L. lumborum: 15.9% vs 18.1%; P<0.05). Outdoor rabbit hind leg meat was characterized by lower water (74.5% vs 75.1%; P<0.01) and higher protein (22.9% vs 22.6%; P<0.01) and fat (1.4% vs 1.1%; P<0.01) contents; lipids were lower in SFA and higher in MUFA. Outdoor rearing seems to be a possible alternative housing system that allays the ethical concerns of modern consumers while also providing good meat quality.

Effect of turmeric powder (Curcuma longa L.) and ascorbic acid on physical characteristics and oxidative status of fresh and stored rabbit burgers

The objective of this study was to evaluate the effect of Curcuma longa powder and ascorbic acid on some quality traits of rabbit burgers. The burgers (burgers control with no additives; burgers with 3.5 g of turmeric powder/100g meat; burgers with 0.1g of ascorbic acid/100g meat) were analyzed at Days 0 and 7 for pH, color, drip loss, cooking loss, fatty acid profile, TBARS, antioxidant capacity (ABTS, DPPH and FRAP) and microbial growth. The addition of turmeric powder modified the meat color, produced an antioxidant capacity similar to ascorbic acid and determined a lower cooking loss than other formulations. Turmeric powder might be considered as a useful natural antioxidant, increasing the quality and extending the shelf life of rabbit burgers.

Effect of genotype and rearing system on the native immunity and oxidative status of growing rabbits

Abstract To investigate the effects of genotype and rearing system on innate immunity and oxidative status, 40 weaned commercial hybrid (H) rabbits and 40 rabbits of a local population (LP) were assigned to two rearing systems: cage (17 rabbits/m2) and pen (1x2 m, 10 rabbits/m2). Rearing H rabbits in pen resulted in a higher lysozyme concentration (P<.05), an increased bactericidal activity (P<0.05) and a lower complement haemolytic activity (P<0.05) with respect to those reared in cage. Opposite results were obtained for LP rabbits. In addition, genotype H was more susceptibility to inflammation than LP in pen, whereas genotype LP suffered more in cage. A higher lipid (P<0.05) and protein (P<0.05) oxidation was found in Longissimus dorsi and Biceps femoris of rabbits H in pen compared to those in cage. An opposite trend was observed for LP rabbits. In cage, LP rabbits were more susceptible to oxidative stress than H rabbits. Taken together, our results show that immune and oxidative resistance ascribed to a specific genotype are important factors to be considered when a specific rearing system should be adopted. Considering this, rabbit LP seem to better adapt to larger spaces during growing.

Effect of stocking density and group size on growth performance, carcass traits and meat quality of outdoor-reared rabbits

The effect of stocking density (16 rabbits/m(2), 5 rabbits/m(2), 2.5 rabbits/m(2), n = 60, Experiment 1) and group size (4 rabbits/cage, 8 rabbits/cage, 16 rabbits/cage, n = 88, Experiment 2) on productive performance, carcass and meat quality of a slow-growing rabbit population reared outdoors was investigated in two experiments. The highest stocking density induced the highest skin percentage. Lower stocking densities showed lower lightness of Biceps femoris and higher redness of Longissimus lumborum muscles. Four rabbits/cage group (Experiment 2) showed the highest daily weight gain and slaughter weight and the lowest skin percentage. The muscles of 16 rabbits/cage showed significantly higher pHu than 8 and 4 rabbits/cage. BF of 16 and 4 rabbits/cage showed higher L* value. Productive performance and meat quality of rabbits reared outdoors improved in low group size while stocking density needs more experiments. The best combination of density, group size and total available surface that showed the best production and carcass traits was of 5 rabbits/m(2), 4 rabbits/cage, and 0.8m(2).

Relationship between habitat, densities and metabolic profile in brown hares (Lepus europaeus Pallas)

Abstract Some habitat traits and haematic parameters were studied to understand the relationships between the hare densities, habitat characteristics and physiological and nutritional condition of the animals. A total of 33 protected areas, reserved for wild game reproduction, located in the Province of Florence (Central Italy), were monitored during a 2-year period. In each protected area the hares were submitted to census. The habitat features of the protected areas were studied and the following parameters were categorised: altitude; cleared-land/total-land ratio; main exposure; main ground composition; water availability; main slope; anthropogenic presence; predator presence; wooded borders; presence of trees and shrubs; surveillance against hunting; demographic predator control; kind of cultivation; unharvested crops for game. After the census the hares were captured for translocation outside in “free” hunting areas. During capture the hares were put in darkened, wooden capture-boxes and remained inside for a variable period of time (10min to 3h). A sample of 3 to 7 hares, captured per year and per each protected area, were removed from the boxes (physically restrained, with covered eyes) for blood sample collection, sex, age and live weight determination. The following analyses were performed on frozen plasma samples: ALanine aminoTransferase (ALT), ASpartate aminoTransferase (AST), glucose, cholesterol, Blood Urea Nitrogen (BUN), Ca, P, Mg, Na, K, and Cl concentrations. The relationship between hare density and habitat characteristics was analysed by single regressions analysis. Then the habitat characteristics were subjected to multivariate analysis in relationship to hare body condition. The haematic parameters were analysed by least square means considering habitat traits, animal density, age and sex, as main categorical factors, interaction sex*age, and “pregnant and non-reproducing” nested within sex. Results showed that the highest density and best body conditions can be found in highlands, open fields with low tree presence and wooded borders, medium mixture soils, scarce predator presence and limited anthropogenic presence and with abundant water availability and shrubbiness. The study of the absolute values of metabolic profile, indicator of the physiological and nutritional condition of the reared animals, did not show any nutritional winter deficiency in wild hares and, as census data, should be repeated for several years since, probably, only their variations can be used as indicators of preliminary problems.

Genetic Variability in Bracco Italiano Dog Breed Assessed by Pedigree Data

The Bracco Italiano is one of the oldest pointing dog breed, used for hunting ever since the Renaissance time. The complete electronic record of the breed was downloaded from the ENCI database [whole population (WP) = 24,613 animals registered since 1970 to 2011] with the aim to estimate genetic variability in Bracco Italiano dog breed using pedigree records. Up to 97% of the individuals had registered parents and 86% registered grandfathers. Average generation interval was 4.68±0.545 for stallions and 4.08±0.321 year for dams. Reference population (RP) was defined as the population of interest that include living reproductive animals approaching the last three generations and include 9006 dogs of which 34% were inbreds. The number of ancestors was 564 in WP and 188 in RP, while the effective number of ancestors was 46 and 34 respectively. To explain 50% of the genetic variability, a total of 18 and 9 ancestors enough, respectively in the WP and RP. The average inbreeding coefficient in the RP resulted 6.7% while the average increase in inbreeding was estimated to be 1.29% (Ne=38.86). Nevertheless a regular monitoring of genetic variability of the population is important and must be adopted, in order to avoid the danger of an excessive increase of inbreeding in the future, which would result in significant inbreeding depression and in significant loss of genetic variation.

Habitat use and home range traits of resident and relocated hares (Lepus europaeus, Pallas)

The aim of the study was to identify the habitat preferences and home range of resident and relocated brown hares during the nohunting period. The trial was carried out in a protected area and in a free-hunting territory (FHT), both located in the Florence province. During the captures, 21 hares were equipped with a necklace radio-tag: seven hares (resident group) were released in the same area of capture and 14 hares (relocated group) were relocated in six different locations within the FHT. The effect of the place of release was analysed by ANOVA and/or nonparametric methods. Results showed that the home ranges of the resident group were characterized by a greater amount of fallow land and shrub land than of the relocated group (P<0.05). Home range sizes and maximum distances from the releasing sites were greater in the relocated group. Resident hares preferred landscape characterized by a higher density of patches (152 vs. 70 n/100 ha), patch richness (43 vs. 12 n/100 ha), and patch area (4703 vs. 8142 m2) than the relocated hares (P<0.01). The landscape structure indices, the home range sizes and the maximum distance from the releasing sites suggest that the relocated hares, even if released in suitable habitats, will move from their releasing point to look for better habitats. The landscape with the most complexity is preferred by the resident hares. This result should be considered when a project is programmed to reintroduce this lagomorph into a territory, or when it is necessary to improve the dynamics of a natural population.

Investigation on the genetic variability of the american Pit Bull Terrier dogs belonging to an Italian breeder using microsatellite markers and genealogical data

The genetic variability of 18 American Pit Bull Terriers bred in Italy was studied using 21 STR markers from the panels recommended for the 2006, 2008 and 2010 ISAG canine comparison test and the genealogical information. As expected, all statistical analysis showed a reduced genetic variability. It is there-fore recommended greater attention in the programming of mating with an increase of gene flow among farmers, which would reduce the average inbreeding in the population and increase genetic variability.

Relationship between Anti-European Brown Hare Syndrome Serological Titers and Brown Hare (Lepus europaeus Pallas) Densities

Thirty-three protected wild game reproduction areas, located in the province of Florence (Central Italy), were monitored for habitat characteristics and hare census over a period of 2 years. A total of 172 hares was captured, checked for sex, and age, and blood samples were taken. Serum samples were analyzed by competitive ELISA test for detection and titration of anti-European brown hare syndrome virus (EBHSV) antibodies. Results showed that EBHSV seropositive hares from areas with high and medium population densities had higher antibody titers than those coming from low-density areas and that adults showed lower values than young animals. Anti-EBHSV antibody levels were inversely related to the distances between protected areas and private hunting areas while a high density of protected areas was not associated with any similarity in the values or prevalence of EBHSV.

Carcass traits and meat quality of two different rabbit genotypes

To evaluate the effect of genotype on carcass traits and meat quality, sixty-four rabbits belonging to two genotypes (slow growing local population, LP; commercial hybrids, HY) were used. Rabbits were weaned at 35 days of age and slaughtered when they reached 2500 g of live weight, corresponding to 103 and 87 days of age for LP and HY, respectively. Comparing the slaughter traits of two genotypes, LP provided higher dressing out (59.4 vs 56.2%, P<0.01) and skin percentage (16.0 vs 14.2%, P<0.05), lower incidence of full gastrointestinal tract (18.5 vs 22.3%, P<0.01) and higher incidence of head (9.5 vs 8,9%, P<0.05) and kidneys (0.99 vs 0.86%, P<0.05) than HY. Reference carcasses from LP had greater incidence of perirenal fat (2.04 vs 1.12%; P<0.01), loin (21.5 vs 19.2%; P<0.01) and hind leg (34.4 vs 31.6%; P<0.01) than HY. Hind leg meat-to-bone ratio was significantly higher in HY than LP (4.7 vs 3.8%; P<0.01). The comparison between the variances of slaughter weight, chilled carcass weight, kidneys percentage, reference carcass weight, perirenal fat and hind leg percentage showed the greatest variability in the LP. Meat derived from LP showed lower L*, higher a*, b* and C* colour values than HY (P<0.01). In conclusion, LP showed good slaughter traits and favourable meat quality. The great variability observed in the LP could allow to improve the productive performances, however maintaining animal rusticity.