Gisely Naura Venâncio

Evaluation of the stability and antimicrobial activity of an ethanolic extract of Libidibia ferrea

Biofilm is a dense, whitish, noncalcified aggregate of bacteria, with desquamated epithelial cells and food debris creating conditions for an imbalance of resident oral microflora and favoring the destruction of hard and soft tissues by development of caries and gingivitis. The aim of this study was to obtain and characterize an extract of Libidibia ferrea, ex Caesalpinia ferrea L. and to evaluate its feasibility for formulation as a mouthwash, according to current legislation. For this purpose, pH, sedimentation, density, and stability were evaluated, along with microbiological testing of the extract. The microbiological test was used to verify the presence of Staphylococcus aureus, Pseudomonas aeruginosa, fungi, yeasts, coliforms, and minimum inhibitory concentrations of Streptococcus mutans and Streptococcus oralis strains. Characterization, microbiological evaluation, and minimum inhibitory concentration results were tabulated and described using descriptive statistics. The L. ferrea extract showed stable characteristics, product quality, and antibacterial activity against the microorganisms tested irrespective of experimental time intervals. According to these results, it can be concluded that formulation of a mouthwash containing L. ferrea extract to control biofilm is feasible, but further studies are needed.

Antibacterial Activity of Copaiba Oil Gel on Dental Biofilm

Amazonian biodiversity products that have been used for years in folk medicine, have emerged as feasible and promising alternatives for the inhibition of microorganisms in dental biofilm. Copaiba oil, a phytotherapic agent widely used by the Amazonian populations, is known for its antibacterial, anti-inflammatory, anesthetic, healing and antitumor medicinal properties. Objective: The aim of this study was to evaluate the in vitro antibacterial activity of copaiba oil (Copaifera multijuga) gel against strains of Streptococcus sp present in dental biofilm. Materials and Methods: The copaiba oil was obtained and the chemical components were identified. The oil emulsions were formulated and used with the Brain Heart Infusion agar diffusion method with strains of Streptococcus mitis, Streptococcus constellatus and Streptococcus salivarius isolated from patients as well as standard strains of S. mitis (ATCC903), S. mutans (ATCC10449), S. sanguinis (ATCC15300) and S. oralis (ATCC10557). The study groups were as follows: experimental copaiba oil gel, 1% chlorhexidine gel (positive control) and base gel (negative control). The seeded plates were incubated at 37ºC for 12, 24 and 48 hours, respectively. The results obtained were analyzed by Shapiro-Wilk and Friedman Tests (p<0.05) for non parametric data and the Tukey test was used for pH values with 5% level of significance. Results: The experimental copaiba oil gel and 1% chlorhexidine gel showed antibacterial activity against the tested microorganisms. Conclusion: The copaiba oil gel demonstrated antibacterial activity against all the strains of Streptococcus sp tested, suggesting that it can be used for dental biofilm control.

Herbal mouthwash based on Libidibia ferrea: microbiological control, sensory characteristics, sedimentation, pH and density

Introduction Phytotherapy is the study of herbal medicines and their applicability to cure diseases in general, being a therapeutic method which can be used for the prevention and treatment of mouth diseases. Among the herbal studied, the Libidibia ferrea, known as juca or ironwood, is widely used in folk medicine by presenting anti-inflammatory, analgesic, antimicrobial and antipyretic therapeutic properties. Objective To evaluate in vitro pharmacological stability of the Libidibia ferrea extract’s mouthwash (INPA - 228 022). Material and method It was held the mouthwash microbiological control by determining the total number of microorganisms and Salmonella sp, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus; stability characteristics (color, odor, brightness and consistency), sedimentation test (centrifuge), the pH measurement (pH meter) and density evaluation (pycnometer) were analyzed. Result The mouthwash showed to be absent from microorganisms and no changes were observed in the organoleptics and sedimentation characteristics. The average pH values were 6.21, 6.15 and 5.85 at 0, 30 and 60 days, respectively, and 1.029, 1.033 and 1.035 g/ mL density values, respectively, without interfering with the final characteristic of the formulation. Conclusion The mouthwash presented pharmacological stability and quality conditions.

Tratamento de disfunção temporomandibular com placa oclusal: relato de caso

O transtorno da articulacao temporomandibular (ATM) e uma desordem de etiologia multifatorial que envolve os musculos da mastigacao, a ATM, a denticao e estruturas associadas. O objetivo deste trabalho e relatar um caso clinico de tratamento de disfuncao temporomandibular por meio da utilizacao de placa oclusal. A paciente compareceu a clinica da Universidade Nilton Lins, queixando-se de dores orofaciais, na cabeca e nos musculos da mastigacao. Ao exame extraoral de palpacao da ATM percebeu-se a presenca de estalido, crepitacao e deslocamento de disco com reducao em ambos os lados na abertura bucal. Ao exame intraoral, foi observada mordida ‘topo a topo’, desvio mandibular na abertura e fechamento, desgastes dentarios e ausencia do elemento 13. Apos analise dos modelos montados em articulador semi-ajustavel, o diagnostico foi de disfuncao temporomandibular associada ao bruxismo centrico. O tratamento de escolha foi a confeccao de placa oclusal superior rigida em resina acrilica termopolimerizavel. O procedimento executado foi satisfatorio, pois houve reducao da intensidade e frequencia da dor, com melhora na qualidade de vida da paciente. Descritores: Transtornos da Articulacao Temporomandibular; Musculos da Mastigacao; Placas Oclusais.

Tratamento de fratura dentária em atleta: Relato de caso

Devido ao crescimento de praticantes de atividade esportiva houve um aumento do indice de traumatismo dentario. Desta maneira, o presente trabalho tem como objetivo descrever o caso clinico de um atleta com fratura dentaria do elemento 11. Paciente de 14 anos, sexo masculino, compareceu a Clinica de Odontologia da Universidade Nilton Lins, queixando-se de fratura de dente anterior durante a pratica de esporte. Apos anamnese e exame clinico, optou-se pela restauracao direta em resina composta do elemento fraturado e confeccao do protetor bucal, como plano de tratamento, a fim de evitar traumas posteriores. Pode-se concluir que a restauracao em resina composta direta do dente anterior fraturado foi realizada de maneira satisfatoria, restabelecendo funcao e estetica, alem da harmonia do sorriso e a autoestima do paciente e que o uso de protetores bucais e importante para a prevencao de futuros traumas dentarios durante a pratica desportiva. Descritores: Traumatismos em Atletas; Resinas Compostas; Traumatismos Dentarios; Protetores Bucais.

Fechamento de diastemas anteriores com resina composta direta: Relato de caso

Introducao: Os diastemas sao espacos interdentais que ocorrem nos arcos superior e inferior, tanto em dentes anteriores como em dentes posteriores, sendo mais frequentes em dentes anterossuperiores. Sua etiologia e multifatorial e deve ser avaliada com cautela para que o tratamento correto seja realizado e concluido com sucesso. Objetivo: Dessa maneira, o presente artigo possui como objetivo relatar um caso clinico em que se optou pela confeccao de facetas em resina composta direta para o fechamento dos diastemas. Caso clinico: Paciente de 21 anos, procurou atendimento no curso de graduacao em Odontologia da Universidade Nilton Lins queixando-se do espaco que havia entre seus incisivos superiores. Apos exame clinico foi sugerida a confeccao de facetas em resina composta pela tecnica direta. Conclusao: Por meio deste caso clinico conclui-se que a realizacao de facetas utilizando materiais adesivos se destaca por reduzido tempo de trabalho e baixo custo. Mostrando ser uma tecnica eficaz no restabelecimento dos aspectos funcionais e esteticos do sorriso.

Biocompatibility of propolis in subcutaneous tissue of rats: a possible biomaterial for cavity cleansing

OBJECTIVE: The aim of this study was to histopathologically analyze, in subcutaneous connective tissue in rats, a propolis solution for cavity cleansing and its toxicity through hemolytic and Artemia franciscana tests. METHODS: Fifteen male rats were selected and randomly distributed in three experimental periods (07, 30 and 45 days), in which each animal received the four treatment groups in rounds: Group I – Propolis I; Group II – Propolis II; Group III – Calcium Hydroxide Water and Group IV – 2% Chlorexidine; the sides of the tube were the control group. The data were analyzed using descriptive statistics. The results showed, in terms of biocompatibility, that all materials presented a significant reduction of the inflammatory infiltrate and an increase of the thickness in the collagen fibers. It may be suggested, in decreasing order of biocompatibility, the use of following materials: calcium hydroxide-water, 2% chlorexidine, propolis I and propolis II. RESULTS: In the cytotoxicity test using A. franciscana, the propolis extract showed high toxicity when tested at concentrations and in the hemolytic activity test the propolis I extract showed greater activity than propolis II. CONCLUSION: The present study suggests the use of propolis as a cavity cleansing solution for shallow and medium cavities similar to 2% chlorexidine.

Cárie dentária em indivíduos entre 35 e 44 anos do município de Manaus-AM: análise dos dados do Projeto SB Brasil 2010

A carie, o maior problema de saude em odontologia, tem como principal fator etiologico o acumulo de biofilme dental, uma doenca que, na ausencia de tratamento, progride ate destruir totalmente a estrutura dentaria, sendo resultante do desequilibrio nos processos de desmineralizacao e remineralizacao. O objetivo deste trabalho foi analisar os resultados do Indice CPO-D na faixa etaria de 35-44 anos na populacao de Manaus coletados do levantamento epidemiologico de âmbito nacional da area de saude bucal, intitulado Projeto SB Brasil 2010. Os resultados mostram que o estado de saude bucal nos individuos 35-44 anos de idade reflete o impacto acumulado de politicas de prevencao e tratamento, sendo que a expressiva melhora nas condicoes dentarias e um indicador importante de que as mudancas na ocorrencia da carie dentaria descrita para a populacao infantil brasileira tambem estao alcancando a populacao adulta. Conclui-se que a cidade de Manaus, Amazonas, possui o maior indice CPO-D dentre as capitais da Regiao Norte, equiparando-se a Porto Velho, Rio Branco e Boa Vista. Descritores: Carie Dentaria; Adulto; Inqueritos de Saude Bucal.

Dentin Cleaning Ability of an Amazon Bioactive: Evaluation by Scanning Electron Microscopy

The role of dentin cleaning is to remove debris that may impair adaptation and marginal sealing, quantitatively reducing microorganisms. The aim of this study was to investigate through scanning electron microscopy (SEM) the morphology of the dentin surface, cut and treated with copaiba oil emulsions (CO) and suspension of ethanol extract of propolis (EP). Twenty four upper pre-molars teeth, divided into eight groups (n=3), were used: G1: no cleaning, G2: air/water spray, G3: 10% CO, G4: 10% CO + A, G5: 30% CO, G6: 30% CO + A, G7: 1% EP, G8: 2% Chlorhexidine. The specimens were dentin discs (1 mm Ø). The SEM photomicrographs were classified and the results were: G1 - Debris dentin on the entire image / countless microorganisms, G2 and G7 - 50-100 debris / countless microorganisms and G3, G4, G5, G6 and G8 - 0-50 debris / countable microorganisms (50-100 colonies). Conclusion: The present results suggest that copaiba oil emulsions (CO) and suspension of ethanol extract of propolis (EP) have feasibility to be used as bioactive dental cleaning agents.

Reabilitação estética anterior com sistema IPS e.max: relato de caso

The use of metal free crowns on anterior teeth have been widely used in cosmetic rehabilitation cases. Modern ceramic systems have high color stability, wear resistance, aesthetic similar to natural teeth and large clinical success rate, if well executed. The aim of this study was to report a case of aesthetic rehabilitation in upper front teeth with all-ceramic crowns associated with intraradical retainers. The adopted treatment plan for the event was the removal of the old cores, endodontic retreatment of the elements, cementing new retainers, gingivoplasty and cementation of crowns IPS e.max. The proposed treatment allowed restore aesthetics, function and return self-esteem, reported by the patient. The previous rehabilitation with IPS e.max can be an alternative that combines aesthetic qualities with excellent mechanical properties. Descriptors: Esthetics; Dental Prosthesis; Dental Porcelain.