Giuliano Zaghini

The effect of journey time and stocking density during transport on carcass and meat quality in rabbits

Four hundred and fifty hybrid commercial rabbits (half males and half females) at the end of their productive cycle (82 days old) were transported in cages (98×52×24cm, length×width×height) at high or low density (75.5 or 49.0kg/m(2) - 15 or 10 animals per cage) on an uncovered truck for 1, 2 or 4h. Live weight before and after transport as well as slaughter data were recorded for each rabbit. A subset of 180 carcasses were evaluated for meat quality on the basis of meat pH, colour (CIELab system), cooking loss, drip loss and shear force. As a representative of the whole carcass muscle/bone ratio, the left hind leg was separated, dissected and its meat analysed in order to determine its water, protein, ash and lipid content. It was found that a longer journey significantly increases the live weight losses (3.3% vs. 2.0% vs. 1.6% for 4, 2 or 1h, respectively; P<0.001), as a result not only of urine and fecal losses, but also of a decrease in carcass weight (P<0.01) during transport. Ultimate pH (pH(u)) was higher and pH drop lower in rabbits transported for 4h compared to those transported for 2h (P<0.05). Moreover, the meat from animals that had undergone the longest journey was more purple-red (P<0.05), darker (P<0.0001), and firmer when raw and showed less cooking loss than meat from those that underwent shorter journeys. Transport density did not influence any of the considered parameters and there was no interaction between transport time and density.

Effect of feeding a selected combination of galacto-oligosaccharides and a strain of Bifidobacterium pseudocatenulatum on the intestinal microbiota of cats

OBJECTIVE To evaluate the growth kinetics of a strain of Bifidobacterium pseudocatenulatum (BP) on 4 oligo- or polysaccharides and the effect of feeding a selected probiotic-prebiotic combination on intestinal microbiota in cats. ANIMALS 10 healthy adult cats. PROCEDURES Growth kinetics of a strain of cat-origin BP (BP-B82) on fructo-oligosaccharides, galacto-oligosaccharides (GOS), lactitol, or pectins was determined, and the combination of GOS and BP-B82 was selected. Cats received supplemental once-daily feeding of 1% GOS-BP-B82 (10(10) CFUs/d) for 15 days; fecal samples were collected for analysis the day before (day 0) and 1 and 10 days after the feeding period (day 16 and 25, respectively). RESULTS Compared with the prefeeding value, mean fecal ammonia concentration was significantly lower on days 16 and 25 (288 and 281 μmol/g of fecal dry matter [fDM], respectively, vs 353 μmol/g of fDM); fecal acetic acid concentration was higher on day 16 (171 μmol/g of fDM vs 132 μmol/g of fDM). On day 16, fecal concentrations of lactic, n-valeric, and isovaleric acids (3.61, 1.52, and 3.55 μmol/g of fDM, respectively) were significantly lower than on days 0 (5.08, 18.4, and 6.48 μmol/g of fDM, respectively) and 25 (4.24, 17.3, and 6.17 μmol/g of fDM, respectively). A significant increase in fecal bifidobacteria content was observed on days 16 and 25 (7.98 and 7.52 log(10) CFUs/g of fDM, respectively), compared with the prefeeding value (5.63 log(10) CFUs/g of fDM). CONCLUSIONS AND CLINICAL RELEVANCE Results suggested that feeding 1% GOS-BP-B82 combination had some positive effects on the intestinal microbiota in cats.

Effects of a soybean-free diet supplied to Italian heavy pigs on fattening performance, and meat and dry-cured ham quality

The aim of this study was to investigate the effects of a diet containing non-conventional (i.e. alternative to soybean meal) vegetable protein sources on fattening performance, and meat and dry-cured ham quality of heavy pigs. Fifty-six (Landrace x Large White) castrated males with an initial average body weight of 50 kg were allocated to two experimental groups: a control group in which pigs received a traditional soybean meal-based diet, and a treatment group in which soybean meal was replaced by vegetable protein sources (i.e. sunflower meal, potato protein, corn gluten feed, faba beans and dehydrated alfalfa meal), mainly locally grown and not genetically modified. Pigs were slaughtered at approximately 160 kg body weight. Dietary treatment had no significant effect on fattening performance, or meat, fat or dry-cured ham properties. Results suggest that it is possible to feed heavy pigs a soybean-free diet without impairing fattening performance or the quality of meat and Italian PDO (Protected Designation of Origin) hams.

Growth parameters, behavior, and meat and ham quality of heavy pigs subjected to photoperiods of different duration

To attain a good level of animal welfare, pigs require a sufficient environmental illumination. Therefore, minimum levels for light duration and light intensity have been set up by the European legislation (Directive 2008/120). An experimental trial was designed to determine whether an increased duration of the photophase (up to 16 h of light per day) could modify the behavior, productive parameters, and meat and ham quality of Italian heavy pigs. Forty crossbred (Large White × Landrace) castrated males pigs (26 kg initial average BW) intended for Protected Designation of Origin (according to European Union Regulation 1151/2012, ) dry-cured ham production were raised according to Parma ham production rules up to the weight of 160 kg. Pigs were homogeneously allotted to 2 experimental groups, each comprising 20 pigs. The short photoperiod (SP) group received the minimum mandatory number of hours of light per day (corresponding to 8 h/d), whereas the long photoperiod (LP) group was subjected 16 h of light per day during the whole production cycle. Light intensity was maintained at 40 lux (i.e., the minimum mandatory level) for both the experimental groups. Growth and slaughtering parameters, carcass traits, fatty acid composition, meat and dry-cured ham quality, and animal behavior were assessed. Pigs in the LP group showed a greater live weight and carcass weight compared to the SP group ( = 0.005 and = 0.007, respectively). Similarly, hams obtained from the LP group were significantly heavier and their weight losses during the dry-curing period were reduced ( < 0.01) when compared to the SP group. No significant differences were detected between the experimental groups as concerns meat and ham quality or fatty acid composition of the subcutaneous fat. Pigs in the LP group spent more time resting and less time pseudo-rooting ( < 0.01). Our results indicate that, given an appropriate dark period for animal rest, an increased duration of the photoperiod, even at the lower mandatory light intensity level, can favorably affect growth parameters of heavy pigs without any negative effect on animal behavior, carcass traits, or meat or long-cured ham quality. Therefore, rearing pigs in semidarkness should be considered as a baseless practice, contrary to animal welfare.

Consequences of behaviour on productive performances of rabbits reared in pens

Riassunto Effetti del comportamento sulle prestazioni produttive di conigli allevati in colonia. Scopo della presente ricerca è stato di verificare i riflessi del comportamento sulle prestazioni produttive di conigli in accrescimento stabulati in gabbie bicellulari o in parchetti collettivi su fondo di rete, a pari densità (15 capi/m2) e a diversa numerosità (15 e 30 soggetti per gruppo). Trentasei conigli maschi, 12 per ciascun gruppo sperimentale, scelti a caso in un lotto complessivo di 212 animali sottoposti a sperimentazione, sono stati videoregistrati per un periodo di 16 ore nell’ultima settimana di allevamento, al fine di poterne evidenziare il tempo dedicato a diversi pattern comportamentali. Gli incrementi ponderali sono stati monitorati mediante pesate individuali ogni due settimane, dallo svezzamento (32 d) alla macellazione (82 d). I riscontri dimostrano, rispetto ai soggetti allevati in gabbie tradizionali, una maggiore attività e aggressività nei conigli allevati in colonia, indipendentemente dalla numerosità adottata. Si è potuta evidenziare una correlazione significativa (P<lt;0,01) fra le attività di interazione e di aggressione o di fuga ed i minori accrescimenti realizzati dai conigli allevati in parchetti. In tal senso, la stabulazione alternativa in colonia potrebbe rivelarsi elemento inscindibilmente correlato con situazioni “stressanti” che condizionano negativamente le prestazioni produttive.

A new in vitro method to evaluate digestibility of commercial diets for dogs

Abstract The aim of the present study was to develop a new in vitro method for evaluating the digestibility of commercial diets for dogs. First, in order to develop the in vitro method, the digestibility of four commercial diets for dogs was evaluated through several in vitro trials and results were compared with those that were retrieved from the literature. The in vitro method that was developed consists of two incubation phases, a first lasting 2h and taking place in the presence of pepsin, gastric lipase and HCl (gastric phase) and a second 4h one with pancreatin and bile salts (intestinal phase). Later, digestibility of 16 extruded diets for dogs was evaluated both in vivo with adult dogs and in vitro. There was a close linear relationship between in vivo total tract and in vitro dry matter digestibility (r2 = .81), whereas accuracy of crude protein digestibility using the in vitro method was lower (r2 = .51). Linear regression accuracy for ether extract and starch digestibility was low, but the digestibility results obtained with the in vitro method (95.3 and 98.7% for ether extract and starch, respectively) were very close to those from the in vivo trial (average digestibility of ether extract and starch was 94.8 and 99.1%, respectively). The present in vitro method has proved to be a relatively simple, quick procedure for predicting the digestibility of commercial diets for dogs. The utilisation of such a method may significantly reduce the need for in vivo digestion trials with dogs.

Fumonisin B1 and B2 in dry dog food: preliminary study on commercial samples

A reliable liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the identification and quantification of fumonisin B1 (FB1) and fumonisin B2 (FB2) in complete and complementary formulations of dry dog foods has been optimised and validated. The sample preparation consists of an extraction step followed by immunoaffinity clean-up. Method performance characteristics were evaluated spiking blank samples on multiple levels in four replicates. The method showed appropriate performance characteristic: good values of recovery (>95.9%) and precision (RSD <6.8%), as well as satisfying linearity of calibration curves (r2≥0.99). The limit of quantification (LOQ) and detection (LOD) were 0.100 µg/g and 0.005 µg/g, respectively, both in complete and complementary dry dog foods and both for FB1 and FB2. This method was applied to 41 commercial samples in order to test its efficacy and gain some preliminary data about fumonisin contamination in dog food available in the Italian market. FB1 and FB2 were d...

Preliminary Investigation of the Incidence of Obesity in a Canine Population in the USA

Obesity represents the most common nutritional pathology in companion animals. At least one-third of the canine population is estimated to be overweight or obese. The aim of the present study was to evaluate the nutritional status of a canine population in the USA and assess its correlation with some specific individual factors. During a period of approximately 2 months, data concerning nutritional status, dietary management, and clinical situation were collected for 158 adult dogs. Half of the animals were overweight or obese with an average body condition score (BCS) of 3.5/5. Factors such as age, neutering, hormone and chronic arthritic disorders, and breed were significantly positively correlated with BCS. On the contrary, gender, the presence of other animals and type of food provided did not correlate with BCS. Despite the relatively low number of dogs involved, these results confirm that overweight status affects a large portion of the canine population in the USA and is influenced mainly by endogenous factors.