Irene Cipollini

Effect of tannins on growth performance and intestinal ecosystem in weaned piglets

Tannins are natural polyphenolic compounds that can reduce digestibility of dietary protein but also display antibacterial effects. The present study investigated, in vitro and in vivo, the effect of different levels of tannins (using a chestnut wood extract containing 75% tannins) on growth performance, intestinal microbiota and wall morphology in piglets. During a 24 h in vitro caecal fermentation, the utilisation of tannins at 0.75, 1.5, 3, and 6 g/l significantly reduced total gas production and concentrations of ammonia and volatile fatty acids and increased viable counts of enterococci and coliforms. When fed to piglets at 1.13, 2.25, and 4.5 g/kg, tannins significantly improved feed efficiency and reduced caecal concentrations of ammonia, iso-butyric, and iso-valeric acid. Viable counts of lactobacilli tended to be increased by tannins in the jejunum, while bacterial caecal counts were not affected. Depth of ileal crypts tended to decrease in piglets fed tannins at 2.25 and 4.5 g/kg. The present study showed that feeding weaned piglets with a tannin-rich wood extract can result in improved feed efficiency and reduction of intestinal bacterial proteolytic reactions. The growth-enhancing effect that tannins had on enterococci and coliforms under in vitro conditions deserves further investigation.

Effect of feeding a selected combination of galacto-oligosaccharides and a strain of Bifidobacterium pseudocatenulatum on the intestinal microbiota of cats

OBJECTIVE To evaluate the growth kinetics of a strain of Bifidobacterium pseudocatenulatum (BP) on 4 oligo- or polysaccharides and the effect of feeding a selected probiotic-prebiotic combination on intestinal microbiota in cats. ANIMALS 10 healthy adult cats. PROCEDURES Growth kinetics of a strain of cat-origin BP (BP-B82) on fructo-oligosaccharides, galacto-oligosaccharides (GOS), lactitol, or pectins was determined, and the combination of GOS and BP-B82 was selected. Cats received supplemental once-daily feeding of 1% GOS-BP-B82 (10(10) CFUs/d) for 15 days; fecal samples were collected for analysis the day before (day 0) and 1 and 10 days after the feeding period (day 16 and 25, respectively). RESULTS Compared with the prefeeding value, mean fecal ammonia concentration was significantly lower on days 16 and 25 (288 and 281 μmol/g of fecal dry matter [fDM], respectively, vs 353 μmol/g of fDM); fecal acetic acid concentration was higher on day 16 (171 μmol/g of fDM vs 132 μmol/g of fDM). On day 16, fecal concentrations of lactic, n-valeric, and isovaleric acids (3.61, 1.52, and 3.55 μmol/g of fDM, respectively) were significantly lower than on days 0 (5.08, 18.4, and 6.48 μmol/g of fDM, respectively) and 25 (4.24, 17.3, and 6.17 μmol/g of fDM, respectively). A significant increase in fecal bifidobacteria content was observed on days 16 and 25 (7.98 and 7.52 log(10) CFUs/g of fDM, respectively), compared with the prefeeding value (5.63 log(10) CFUs/g of fDM). CONCLUSIONS AND CLINICAL RELEVANCE Results suggested that feeding 1% GOS-BP-B82 combination had some positive effects on the intestinal microbiota in cats.

A new in vitro method to evaluate digestibility of commercial diets for dogs

Abstract The aim of the present study was to develop a new in vitro method for evaluating the digestibility of commercial diets for dogs. First, in order to develop the in vitro method, the digestibility of four commercial diets for dogs was evaluated through several in vitro trials and results were compared with those that were retrieved from the literature. The in vitro method that was developed consists of two incubation phases, a first lasting 2h and taking place in the presence of pepsin, gastric lipase and HCl (gastric phase) and a second 4h one with pancreatin and bile salts (intestinal phase). Later, digestibility of 16 extruded diets for dogs was evaluated both in vivo with adult dogs and in vitro. There was a close linear relationship between in vivo total tract and in vitro dry matter digestibility (r2 = .81), whereas accuracy of crude protein digestibility using the in vitro method was lower (r2 = .51). Linear regression accuracy for ether extract and starch digestibility was low, but the digestibility results obtained with the in vitro method (95.3 and 98.7% for ether extract and starch, respectively) were very close to those from the in vivo trial (average digestibility of ether extract and starch was 94.8 and 99.1%, respectively). The present in vitro method has proved to be a relatively simple, quick procedure for predicting the digestibility of commercial diets for dogs. The utilisation of such a method may significantly reduce the need for in vivo digestion trials with dogs.

Fumonisin B1 and B2 in dry dog food: preliminary study on commercial samples

A reliable liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the identification and quantification of fumonisin B1 (FB1) and fumonisin B2 (FB2) in complete and complementary formulations of dry dog foods has been optimised and validated. The sample preparation consists of an extraction step followed by immunoaffinity clean-up. Method performance characteristics were evaluated spiking blank samples on multiple levels in four replicates. The method showed appropriate performance characteristic: good values of recovery (>95.9%) and precision (RSD <6.8%), as well as satisfying linearity of calibration curves (r2≥0.99). The limit of quantification (LOQ) and detection (LOD) were 0.100 µg/g and 0.005 µg/g, respectively, both in complete and complementary dry dog foods and both for FB1 and FB2. This method was applied to 41 commercial samples in order to test its efficacy and gain some preliminary data about fumonisin contamination in dog food available in the Italian market. FB1 and FB2 were d...

In vitro fermentation of different sources of soluble fiber by dog faecal inoculum

In vitro fermentation of different sources of soluble fiber by dog faecal inoculum G. Biagi & I. Cipollini & G. Zaghini Published online: 7 August 2008 # Springer Science + Business Media B.V. 2008

Preliminary Investigation of the Incidence of Obesity in a Canine Population in the USA

Obesity represents the most common nutritional pathology in companion animals. At least one-third of the canine population is estimated to be overweight or obese. The aim of the present study was to evaluate the nutritional status of a canine population in the USA and assess its correlation with some specific individual factors. During a period of approximately 2 months, data concerning nutritional status, dietary management, and clinical situation were collected for 158 adult dogs. Half of the animals were overweight or obese with an average body condition score (BCS) of 3.5/5. Factors such as age, neutering, hormone and chronic arthritic disorders, and breed were significantly positively correlated with BCS. On the contrary, gender, the presence of other animals and type of food provided did not correlate with BCS. Despite the relatively low number of dogs involved, these results confirm that overweight status affects a large portion of the canine population in the USA and is influenced mainly by endogenous factors.