Gizo Nakagawara

Blue rubber bleb nevus syndrome: report of a case.

SummaryA 21-year-old man had blue rubber bleb nevus syndrome: 1) hemangiomas of the skin all over the body; 2) hemangiomas of the digestive system; 3) iron-deficiency anemia. A giant mass of hemangiomas in the tranverse colon was resected surgically.

Lymphangioma of the transverse colon treated by transendoscopic polypectomy

This report concerns a 73-year-old man complaining of intermittent, dull, umbilical pain of one months duration. Barium-enema examination and endoscopy revealed a hemispheric cystic tumor at the midportion of the transverse colon, and the presumptive diagnosis was cystic submucosal tumor of the colon. Transendoscopic polypectomy was performed and the bulk of the lesion was locally excised from the colonic mucosa. Histologic examination revealed cavernous lymphangioma originating from the submucosa.

Experimental studies on cryopreservation combined with the cultural process of isolated rat pancreatic islets

Recently many investigators have attempted transplantation of isolated islets of Langerhans in an expectation of therapeutic effects in patients with diabetes. In the course of time, preservation of the islets was found to be a prerequisite for successful transplantation. In the present studies, we used our newly designed method of preserving islets, i.e., cryopreservation combined with the cultural process before and after freezing, and we examined the insulin-releasing activity of the islets and attempted transplantation of the islets so-obtained. As to the insulin-releasing activity, the islets cryopreserved after short-term culture increased insulin output as compared with those cryopreserved without culture. Damage to Langerhans’ islets resulting from isolation and cryopreservation was overcome during the culture. Transplantation of Langerhans’ islets cultured for 3–4 days before and after the cryopreserving process produced favorable results.

A case of granuloma of the ascending colon due to penetration of Trichuris trichiura.

SummaryA 33 year-old woman was admitted with chief complaint of abdominal pain and high fever. A bariumenema showed serration and a tumor was seen in the proximal ascending colon. At laparotomy, a localized tumor about 5 cm in diameter was located in the proximal portion of the ascending colon. The operation was made according to the ileoceal resection. On the macroscopic examination of the resected specimen, a small hole penetrating into the subserosa of the ascending colon was noticed and a tumor measuring approximately 3 x 1.2 x 1 cm was located under the hole. A female worm,Trichuris trichiura, was found to be harbored in the adjacent site of the lesion. Histopathologic examination revealed granulomatous tissue reaction due to penetrating ofTrichuris trichiura. The patient is in good health now 20 months after operation.

Experimental studies on cadaver pancreas preserved by perfusion

SummaryPancreatic transplantation is the most effective therapy of advanced diabetes mellitus. However, the problem is whether the cadaver pancreas can be used as a donor, because the living pancreas cannot be used as a donor from an anatomical standpoint. In case of the cadaver pancreas another problem is coming up that the pancreas proceeds to rapid autolysis. So, brain death is recommended as the most available condition for this transplantation. In this study we investigated the viability of the cadaver pancreas from decapitated rats preserved by the cold perfusion method. The results obtained were as follows:1)The perfusing time affected the ratio of degenerative islets/normal islets. A large number of degenerative islets appeared with long perfusion time.2)The cold (4°C) perfusion method could preserve cadaver pancreas for 5 hours.3)Insulin-secretion of cadaver pancreatic islets preserved for 5 hours under cold perfusion was 60% or more of that of normal pancreatic islets.4)Three hundred islets from cadaver pancreas preserved for 5 hours under cold perfusion were transplanted into the portal vein of streptozotocin-induced diabetic rats. Four of these 11 rats recovered from diabetic state and maintained the non-diabetic state for 20 weeks after transplantation.

The Relationship Between Metastatic Potential and the Expression of Sialyl Lewis A (CA 19–9) and Sialyl Lewis X Antigens in Colorectal Cancer

The relationship between metastatic potential and the expression of sialyl Lea or sialyl Lex antigens was studied in 128 advanced colorectal cancer. Hematogenous metastasis was observed more often in the Grade II-III group of sialyl Lea or sialyl Lex staining than in Grade 0-1. The recurrence rates in the Grade II-III group of sialyl Lex staining was significantly higher than those in the Grade 0-1, and the prognosis in the Grade II-III group of sialyl Lex staining was significantly poorer than that in the Grade 0-1. These results suggested that sialyl Lea and sialyl Lex antigens in colorectal cancer might be involved in the hematogenous metastasis.

Proper Indication of Gastrectomy for the Gastric Carcinomas with Liver Metastasis

In 36 gastric carcinomas with synchronous liver metastasis, the influence of the degrees of liver metastasis and gastrectomy with lymphnode dissection upon the survival, was studied. In nine gastrectomy-patients who had less than seven metastatic liver tumors and underwent radical lymphadeneetomy, more favorable median survival time and 1-year survival were obtained, 456 days and 67%, than in other 20 gastrectomy-patients who received palliative lympadenectomy regardless of the numbers of liver metastasis, as well as than in the sever, patients who were not resectable.

Transplantation of preserved pancreatic islets into the portal vein of rats.

Langerhans islets were isolated from the exocrine pancreata of Wistar rats by the improved collagenase-digestion method. The isolated islets were preserved in a tissue culture medium for seven days. Transplantation of these preserved islets into the portal vein of streptozotocin-induced diabetic rats resulted in a significant reduction of hyperglycemia, polyuria and glucosuria, and a restoration of weight gain. It was found that these effects could be maintained for 16 weeks. In order to normalize the K-values and plasma insulin levels, at least 600 islets had to be transplanted into each diabetic rat.