Glenn Vergauwen
Ghent University
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Publication
Featured researches published by Glenn Vergauwen.
Nature Methods | 2017
Jan Van Deun; Pieter Mestdagh; Patrizia Agostinis; Özden Akay; Sushma Anand; Jasper Anckaert; Zoraida Andreu Martinez; Tine Baetens; Els Beghein; Laurence Bertier; Geert Berx; Janneke Boere; Stephanie Boukouris; Michel Bremer; Dominik Buschmann; James Brian Byrd; Clara Casert; Lesley Cheng; Anna Cmoch; Delphine Daveloose; Eva De Smedt; Seyma Demirsoy; Victoria Depoorter; Bert Dhondt; Tom A. P. Driedonks; Aleksandra M. Dudek; Abdou ElSharawy; Ilaria Floris; Andrew D. Foers; Kathrin Gärtner
We argue that the field of extracellular vesicle (EV) biology needs more transparent reporting to facilitate interpretation and replication of experiments. To achieve this, we describe EV-TRACK, a crowdsourcing knowledgebase (http://evtrack.org) that centralizes EV biology and methodology with the goal of stimulating authors, reviewers, editors and funders to put experimental guidelines into practice.
Scientific Reports | 2017
Glenn Vergauwen; Bert Dhondt; Jan Van Deun; Eva De Smedt; Geert Berx; Evy Timmerman; Kris Gevaert; Ilkka Miinalainen; Veronique Cocquyt; Geert Braems; Rudy Van den Broecke; Hannelore Denys; Olivier De Wever; An Hendrix
Identification and validation of extracellular vesicle (EV)-associated biomarkers requires robust isolation and characterization protocols. We assessed the impact of some commonly implemented pre-analytical, analytical and post-analytical variables in EV research. Centrifugal filters with different membrane types and pore sizes are used to reduce large volume biofluids prior to EV isolation or to concentrate EVs. We compared five commonly reported filters for their efficiency when using plasma, urine and EV-spiked PBS. Regenerated cellulose membranes with pore size of 10 kDa recovered EVs the most efficient. Less than 40% recovery was achieved with other filters. Next, we analyzed the effect of the type of protein assays to measure EV protein in colorimetric and fluorometric kits. The fluorometric assay Qubit measured low concentration EV and BSA samples the most accurately with the lowest variation among technical and biological replicates. Lastly, we quantified Optiprep remnants in EV samples from density gradient ultracentrifugation and demonstrate that size-exclusion chromatography efficiently removes Optiprep from EVs. In conclusion, choice of centrifugal filters and protein assays confound EV analysis and should be carefully considered to increase efficiency towards biomarker discovery. SEC-based removal of Optiprep remnants from EVs can be considered for downstream applications.
Journal of extracellular vesicles | 2017
Glenn Vergauwen; Bert Dhondt; Jan Van Deun; Evy Timmerman; Kris Gevaert; Geert Braems; Rudy Van den Broecke; Veronique Cocquyt; Hannelore Denys; Olivier De Wever; An Hendrix
Book: ISEV2017 To cite this article: (2017) Abstract Book: ISEV2017, Journal of Extracellular Vesicles, 6:sup1, 1310414, DOI: 10.1080/20013078.2017.1310414 To link to this article: https://doi.org/10.1080/20013078.2017.1310414
OncoPoint, 6th Research seminar, Abstracts | 2018
Edward Geeurickx; Elisa Heyrman; Jan Van Deun; Lien Lippens; Joeri Tulkens; Bert Dhondt; Glenn Vergauwen; Kris Gevaert; Francis Impens; Ilkka Miinalainen; Pieter-Jan Van Bockstal; Thomas De Beer; Marca H. M. Wauben; Ester Nolte-'t Hoen; Johan Swinnen; Sven Eyckerman; Pieter Mestdagh; Jo Vandesompele; Geert Braems; Hannelore Denys; Marc Bracke; Olivier De Wever; An Hendrix
OncoPoint, 6th Research seminar, Abstracts | 2018
Bert Dhondt; Glenn Vergauwen; Jan Van Deun; Edward Geeurickx; Joeri Tulkens; Lien Lippens; Sarah Buelens; Elise De Bleser; Ilkka Miinalainen; Pekka Rappu; Jyrki Heino; Nicolaas Lumen; Olivier De Wever; An Hendrix
Journal of extracellular vesicles | 2018
Bert Dhondt; Glenn Vergauwen; Jan Van Deun; Edward Geeurickx; Joeri Tulkens; Lien Lippens; Ilkka Miinalainen; Pekka Rappu; Jyrki Heino; Nicolaas Lumen; Olivier De Wever; An Hendrix
European Urology Supplements | 2018
Bert Dhondt; Glenn Vergauwen; Jan Van Deun; Edward Geeurickx; Joeri Tulkens; Lien Lippens; Sarah Buelens; Elise De Bleser; Filip Poelaert; Tom Claeys; Ilkka Miinalainen; Pekka Rappu; Jyrki Heino; An Hendrix; Olivier De Wever; Nicolaas Lumen
Elautprijs, Samenvattingen | 2018
Bert Dhondt; Glenn Vergauwen; Jan Van Deun; Edward Geeurickx; Joeri Tulkens; Lien Lippens; Sarah Buelens; Elise De Bleser; Filip Poelaert; Tom Claeys; Ilkka Miinalainen; Pekka Rappu; Jyrki Heino; An Hendrix; Olivier De Wever; Nicolaas Lumen
The Breast | 2017
M. Van Bockstal; N. Van Roy; Glenn Vergauwen; Kathleen Lambein; Veronique Cocquyt; Louis Libbrecht; Hannelore Denys; Marc Bracke; O. De Wever
OncoPoint, 5th Research seminar, Abstracts | 2017
Bert Dhondt; Glenn Vergauwen; Jan Van Deun; Edward Geeurickx; Tom Claeys; Filip Poelaert; Sarah Buelens; Nicolaas Lumen; An Hendrix; Olivier De Wever
