Gökhan Oto

The effect of Plantago major Linnaeus on serum total sialic acid, lipid-bound sialic acid, some trace elements and minerals after administration of 7,12-dimethylbenz(a)anthracene in rats.

The present study was designed to evaluate the effect of Plantago major Linnaeus (PM) extract on serum total sialic acid (TSA), lipid-bound sialic acid (LSA), some trace elements (copper (Cu), zinc (Zn) and iron) and mineral levels (magnesium, calcium and sodium) in Wistar albino rat administrated 7,12-dimethylbenz(a)anthracene (DMBA). Rats were divided into three equal groups (n = 6). Group I comprised the control group, group II was treated with DMBA (100 mg/kg, single dose) and group III was treated with DMBA (100 mg/kg single dose) and aqueous extract of PM 100 mg/kg/day for 60 days. After 60 days, statistical analyses showed that TSA and LSA levels in DMBA and DMBA + PM groups were significantly higher compared to the control group (TSA: p < 0.01, p < 0.05; LSA: p < 0.05, p < 0.05, respectively). Serum Zn levels were decreased in subjects treated with DMBA (p < 0.01) and DMBA + PM (p < 0.05) compared to the control group values. Serum Cu levels were increased in DMBA group and PM-treated group compared to the control group values. The results of this investigation showed that the levels of TSA and LSA changed significantly, which are sensitive markers for detecting the toxic effects of DMBA. On the other hand, observed decline in Zn levels in rats from DMBA + PM group might be due to decreased generation of free radicals and oxidative stress. Results from this study suggest that PM may be partially effective in preventing carcinogenesis initiated by environmental carcinogen DMBA.

Effects of quercetin and surgicel for preventing adhesions after gynecological surgery: A rat uterine horn model

Postoperative pelvic adhesions are significant health care problems causing chronic pelvic pain, infertility and intestinal obstruction after abdominal or pelvic surgery. We investigated the effects of quercetin and Surgicel for the prevention of adhesions after gynecological surgery.

Simultaneous determination of retinol, retinyl palmitate and β-carotene in rat serum treated with 7,12-dimethylbenz[a]anthracene and Hypericum Perforatum L. by high-performance liquid chromatography with diode-array detection

A new and simple high-performance liquid chromatography method was developed and validated for the simultaneous determination of retinol, retinyl palmitate and β-carotene in rat serum treated with Hypericum Perforatum L. and 7,12-dimethylbenz[a]anthracene. Furthermore, vitamin C was determined spectrophotometrically. High-performance liquid chromatography analysis was performed utilizing an Inertsil ODS3 reversed phase column with methanol-acetonitrile-tetrahydrofuran (65:30:5, v/v/v) as mobile phase, at a flow rate of 1.5 mL min−1 and 40°C. Diode-array detection was conducted at 325 and 450 nm for retinol and retinyl palmitate, and β-carotene, respectively with a running time of 26 min. The high-performance liquid chromatography assay and extraction procedure proposed are simple, rapid, sensitive and accurate. This method was then applied to determine the amounts of retinol, retinyl palmitate and β-carotene in rat serum. Results of this study demonstrated that at 60th day in the 7,12-dimethylbenz[a]anthracene-treated group there was a significant decrease (p<0.001), (p<0,01), (p<0.05) in levels of retinol, retinyl palmitate and vitamin C, respectively compared to the control group levels. A significant decrease (p<0.01) in retinyl palimitate was observed in the 7,12 dimethylbenz[a] anthracene + Hypericum Perforatum L. treated group compared to the control group..

Method Validation and Simultaneous Determination of Retinol, Retinyl Palmitate, β-Carotene, α-Tocopherol and Vitamin C in Rat Serum Treated with 7,12 Dimethylbenz[a]Anthracene and Plantago major L. by High- Performance Liquid Chromatography Using Diode-Array Detection

A new and simple high-performance liquid chromatography method was developed and validated for the simultaneous determination of retinol, retinyl palmitate, β-carotene, α-tocopherol and vitamin C in rat serum treated with Plantago Major L. and 7,12 dimethylbenz[a]anthracene. High-performance liquid chromatography analysis was performed utilizing an Inertsil ODS3 reversed phase column with methanol-tetrahydrofuran-water as mobile phase under gradient conditions, at 1.5 mL min(-1) flow rate and 25 °C. Diode-array detection was at 325, 450, 290 and 270 nm (retinol and retinyl palmitate), β-carotene, α-tocopherol and vitamin C, respectively and runnig time 18 min. The high-performance liquid chromatography assay and extraction procedure proposed are simple, rapid, sensitive and accurate. The method was then applied for the determination of retinol, retinyl palmitate, β-carotene, α-tocopherol and vitamin C in rat serum. Results of this study demonstrated that; at 60th day DMBA-treated group, there was a significant decrease in vitamin levels compared to the levels of control group. A significant increase was observed in vitamin levels of 7,12 dimethylbenz[α]anthracene+Plantago Major L.-treated group compared to the DMBA-treated group. Additionally, the results obtained in the study are found to be in agreement with data reported in the literature.

Determination of 7,12-dimethylbenz[a]anthracene in orally treated rats by high-performance liquid chromatography and transfer stripping voltammetry.

A number of polycyclic aromatic hydrocarbons (PAHs) have been shown to be toxicants, and induce carcinogenic and immunotoxic effects. As a model PAH agent, 7,12-dimethylbenz[a]anthracene (DMBA) was the strongest one tested in terms of its biological activities and biotransformation. A new and simple high-performance liquid chromatographic (HPLC) method with diode-array detection at 290 nm was developed and validated for monitoring of DMBA in different matrices (serum, liver and kidney) of rats orally treated with DMBA. Furthermore, the applicability of adsorptive transfer stripping voltammetry (AdTSV) on the pencil-lead graphite electrode to these samples was illustrated using our previously reported data for bulk aqueous solutions of DMBA. HPLC and AdTSV methods, which were compatible with each other, allowed DMBA to be detected down to the levels of 3.82x10-9 M (0.98 ppb) and 6.73x10-9 M (1.73 ppb), respectively. Olive oil solutions of DMBA in dose 50 mg/kg were orally administered. 60 days after a single dose of DMBA, its concentrations in these biological samples from rats were measured by means of both methods. Because of rapid biotransformation, DMBA could not be detected in serum. Only low levels of the compounds were deposited unchanged in kidney whereas its levels were considerably higher in liver. These methods were also applied to the assay whether there is an influence of the intake of aqueous extracts of Hypericum Perforatum L. plant on the parent DMBA levels accumulated in rat tissues.

Investigation of Allium sativum’s Protective Effect on Ovarian Reserve in an Experimental Ovarian Injury Model

We aimed to evaluate the effects of detorsion and Allium sativum (garlic oil) treatment on the ovarian reserve in an ovarian torsion model. Ovarian torsion may lead to loss of ovarian tissue and infertility. It is an experimental rat study that was carried out on 16 sets of ovaries each, one for treatment group and a control group. In the control group, the procedure involved only the sur gically opening and closing the abdomen. Bilateral adnexal torsion/detorsion was performed after a 3-hour ischemia period for the detorsion-onl y g oup. The detorsion + Allium sativum group received a 5 ml/kg dose of Allium sativum intraperitoneally, 2 hours before surgery. After the second surgery, removed ovarian samples were evaluated for follicle counts, damage scores and other parameters. Primordial, preantral, small antral and large antral follicle counts were significantly higher in the detorsion + Allium sativum group. Degeneration, congestion, hemorrhage ,inflammation and total damage scores were significantly elevated in the detorsion only group compared to those for the detorsi on + Allium sativum group. Finally, there was a significant correlation between AMH alterations and postoperative, preantral follicle count (p<0.0 5). As a conclusion detorsion + Allium sativum treatment may be effective in protecting the ovarian reserve after torsion.

Antioxidant properties of Ferulago angulata and its hepatoprotective effect against N-nitrosodimethylamine-induced oxidative stress in rats

Abstract Context: Ferulago angulata (Schlecht.) Boiss. (Apiaceae) (FASB) is used to treat liver diseases and has been used both as food and therapeutics by many cultures for thousands of years because of the natural antioxidant compounds. Objective: This study determines antioxidant properties of FASB flowers, the levels of minerals and vitamins, and also, evaluates the hepatoprotective effect of flowers against N-nitrosodimethylamine (NDMA) induced on liver tissue by assessing antioxidant enzymes and histopathological parameters in Wistar albino rats. Materials and methods: In the study, the rats were divided into six groups of ten. Control, untreated animals were given 0.9% NaCl. Rats were intraperitoneally given NDMA (10 mg/kg) for the first 7 days. FASB methanol extract (150 and 300 mg/kg) was administered orally for 21 days. Results: α-Tocopherol, retinol, ascorbic acid, total antioxidant activity, phenolic and flavonoid contents of FASB were 0.70 ± 0.13, 0.29 ± 0.03 μg/g, 139.32 ± 7.06 μg/100 g, 171.61 ± 6.05 mM ascorbic acid/g, 90.47 ± 4.11 mg GA/g and 37.39 ± 2.85 mg QE/g. DPPH and hydroxyl radical scavenging activity was obtained IC50 67.34 ± 4.14 and 64.87 ± 4.68 μg/mL, respectively. Discussion and conclusion: The results of the study indicated that FASB flowers contain high levels of vitamins, minerals, total antioxidant activity, phenolics and flavonoids. Due to the positive effect on significant changes in antioxidant enzymes of liver tissue and histopathological examination, it is thought that the plant could be used as a hepatoprotective.

The evaluation of serum total sialic acid and lipid-bound sialic acid levels in chronically exposed rats to 7,12-dimethylbenz(a)anthracene and fluoride

In this study, changes in serum total sialic acid (TSA) and lipid-bound sialic acid (LSA) levels were examined in chronically exposed rats to 7,12-dimethylbenz(a)anthracene (DMBA) and fluoride. This study demonstrated that the TSA, LSA levels increased more in DMBA-treated groups compared to the fluoride treated groups. The result obtained has shown that the harmful effect of DMBA which is also causing more cell membrane damage on human and animal health should be taken into consideration.

Effect of exercise on erythrocyte osmotic fragility in high fat diet fed rats during intermittent fasting

High fat including diets are deleterious for general health and deteriorate health body composition. A healthy diet and exercise is advised for maintaining a healthy life. Exercise is protective against many diseases and prevents obesity, osteoporosis and cardiovascular diseases such as hypertension (1). Regular moderate exercise is good for various aspects of health such as mood alleviation (2). However, irregular and exhausting exercises are not protective and even destructive for cardiovascular system and immune system as well as causing musculoskeletal injuries (3).

Antioxidant properties of Rosa pisiformis and its protective effect against isoproterenol-induced oxidative stress in rats / Rosa pisiformis’in antioksidant özellikleri ve izoproterenol ile ratlarda oluşturulan okdidatif strese karşı koruyucu etkisi

Abstract Objective: In this study, Rosa pisiformis was evaluated for its antioxidant activity, vitamin (A, E, C), trace element (Fe, Cu, Zn, Mn, Cr, Se) and mineral (Ca, K, Mg, Na) levels, in addition to its cardioprotective effects on cardiac tissue antioxidant enzymes (GSH-Px, SOD, CAT) during isoproterenol (ISO) - induced oxidative stress. Methods: In the study, vitamin and mineral analysis was carried out using HPLC and ICP-MS method, R. P. antioxidant and antiradical properties were determined using spectrophotometer. Forty wistar albino rats were divided into four groups: control (0.9% NaCl), ISO (100 mg/kg), ISO (100 mg/kg) + R. P. (300 mg/kg) and R. P. (300 mg/ kg). ISO was injected subcutaneously into rats twice at an interval of 24 h for two consecutive days (on 28th and 29th day). R. P. fruit administered daily. The experimental period was continued for 30 days. Results: The results of the study indicated that R. P. fruit and leaves contain high levels of minerals and vitamins and possess antioxidant and antiradical activity and its fruits have positive effects on cardiac tissues. The results are supported with significant changes in antioxidant enzyme levels and histopathological examination. Conclusion: This study demonstrates that R. P. fruits in particular can be used to treat and prevent cardiac diseases. Özet Amaç: Bu çalışmada Rosa pisiformis’in antioksidant aktivite, vitamin (A,E,C), iz element (Fe, Cu, Zn, Mn, Cr, Se) ve mineral (Ca, K, Mg, Na) düzeyleri, bununla birlikte, isoproterenol ile oluşturulan oksidatif stres boyunca, kalp dokusu antioksidan enzimleri (GSH-Px, SOD, CAT) üzerine koruyucu etkileri değerlendirildi. Metod: Çalışmada, vitamin ve mineral analizi HPLC ve ICP-MS metodu kullanılarak gerçekleştirildi. R. P. antioksidant ve antiradikal özellikleri spektrofotometre kullanılarak belirlendi. Kırk wistar albino rat dört guba ayrıldı: Kontrol (0.9% NaCl), ISO (100 mg/kg), ISO (100 mg/kg) + R. P. (300 mg/kg) ve R. P. (300 mg/kg). ISO ratlara 24 saat ara ile 2 gün (28 ve 29. gün) boyunca iki kez subkutan olarak enjekte edildi. R. P. meyvesi günlük olarak uygulandı. Deneysel aşama 30 gün sürdürüldü. Bulgular: Bu çalışmanın sonuçları, R. P. meyve ve yapraklarının yüksek düzeylerde mineral ve vitamin içeriği, antioksidan ve antiradikal aktiviteye sahip olduğu vemeyvelerinin kalp dokusu üzerinde olumlu etkilere sahip olduğunu gösterdi. Bu sonuçlar, antioksidan enzim düzeylerinde ve histopatolojik incelemedeki anlamlı değişiklikler ile desteklendi. Sonuç: Bu çalışma, R. P. meyvesinin özellikle kalp hastalıklarının önlenmesi ve tedavisi için kullanılabileceğini göstermektedir.