Hülya Özdemir

Effect of Ginkgo biloba Extract (EGb 761) on the Healing of Left Colonic Anastomoses in Rat

BackgroundThe aim of this study was to assess the effect of Ginkgo biloba extract (EGb 761) on healing of experimental colonic anastomoses in a rat model.MethodsRats were divided into four groups: postoperative day (POD) 3 untreated control group, POD 3 EGb 761 group, POD 7 untreated control group, and POD 7 EGb 761 group. In the oral EGb 761 groups, the agent was given at 9.6 mg daily per orogastric route using a 4-F fine feeding catheter. We measured bursting pressures and hydroxyproline content and histologically examined the resected anastomoses on POD 3 and POD 7.ResultsThe bursting pressures increased more in the EGb 761 group than in the untreated control group on POD 3, but this difference was not statistically significant. Hydroxyproline content was higher in the EGb 761 group than in the untreated control group on POD 3, and this difference was statistically significant. Anastomosis bursting pressure values and hydroxyproline contents were significantly higher in the EGb 761 group than in the untreated control group on POD 7. Histological examination showed greater fibroblastic activity in the EGb 761 group than in the untreated control group on POD 3. There was no significant difference in anastomotic polimorphonuclear leukocyte, mononuclear cells and blood vessel neodevelopment between the POD 3 groups, but there was significant difference in fibroblastic activity and blood vessel neodevelopment between the POD 7 groups.ConclusionsThese results showed us that EGb 761 administration resulted in enhanced stability of colonic anastomoses during the first postoperative week.

The effect of Plantago major Linnaeus on serum total sialic acid, lipid-bound sialic acid, some trace elements and minerals after administration of 7,12-dimethylbenz(a)anthracene in rats.

The present study was designed to evaluate the effect of Plantago major Linnaeus (PM) extract on serum total sialic acid (TSA), lipid-bound sialic acid (LSA), some trace elements (copper (Cu), zinc (Zn) and iron) and mineral levels (magnesium, calcium and sodium) in Wistar albino rat administrated 7,12-dimethylbenz(a)anthracene (DMBA). Rats were divided into three equal groups (n = 6). Group I comprised the control group, group II was treated with DMBA (100 mg/kg, single dose) and group III was treated with DMBA (100 mg/kg single dose) and aqueous extract of PM 100 mg/kg/day for 60 days. After 60 days, statistical analyses showed that TSA and LSA levels in DMBA and DMBA + PM groups were significantly higher compared to the control group (TSA: p < 0.01, p < 0.05; LSA: p < 0.05, p < 0.05, respectively). Serum Zn levels were decreased in subjects treated with DMBA (p < 0.01) and DMBA + PM (p < 0.05) compared to the control group values. Serum Cu levels were increased in DMBA group and PM-treated group compared to the control group values. The results of this investigation showed that the levels of TSA and LSA changed significantly, which are sensitive markers for detecting the toxic effects of DMBA. On the other hand, observed decline in Zn levels in rats from DMBA + PM group might be due to decreased generation of free radicals and oxidative stress. Results from this study suggest that PM may be partially effective in preventing carcinogenesis initiated by environmental carcinogen DMBA.

Effects of quercetin and surgicel for preventing adhesions after gynecological surgery: A rat uterine horn model

Postoperative pelvic adhesions are significant health care problems causing chronic pelvic pain, infertility and intestinal obstruction after abdominal or pelvic surgery. We investigated the effects of quercetin and Surgicel for the prevention of adhesions after gynecological surgery.

Investigation of Allium sativum’s Protective Effect on Ovarian Reserve in an Experimental Ovarian Injury Model

We aimed to evaluate the effects of detorsion and Allium sativum (garlic oil) treatment on the ovarian reserve in an ovarian torsion model. Ovarian torsion may lead to loss of ovarian tissue and infertility. It is an experimental rat study that was carried out on 16 sets of ovaries each, one for treatment group and a control group. In the control group, the procedure involved only the sur gically opening and closing the abdomen. Bilateral adnexal torsion/detorsion was performed after a 3-hour ischemia period for the detorsion-onl y g oup. The detorsion + Allium sativum group received a 5 ml/kg dose of Allium sativum intraperitoneally, 2 hours before surgery. After the second surgery, removed ovarian samples were evaluated for follicle counts, damage scores and other parameters. Primordial, preantral, small antral and large antral follicle counts were significantly higher in the detorsion + Allium sativum group. Degeneration, congestion, hemorrhage ,inflammation and total damage scores were significantly elevated in the detorsion only group compared to those for the detorsi on + Allium sativum group. Finally, there was a significant correlation between AMH alterations and postoperative, preantral follicle count (p<0.0 5). As a conclusion detorsion + Allium sativum treatment may be effective in protecting the ovarian reserve after torsion.

The evaluation of serum total sialic acid and lipid-bound sialic acid levels in chronically exposed rats to 7,12-dimethylbenz(a)anthracene and fluoride

In this study, changes in serum total sialic acid (TSA) and lipid-bound sialic acid (LSA) levels were examined in chronically exposed rats to 7,12-dimethylbenz(a)anthracene (DMBA) and fluoride. This study demonstrated that the TSA, LSA levels increased more in DMBA-treated groups compared to the fluoride treated groups. The result obtained has shown that the harmful effect of DMBA which is also causing more cell membrane damage on human and animal health should be taken into consideration.

The treatment of metastatic prostate carcinoma with BNCT in the ITU TRIGA MARKII reactor on rat model

Received 06-12-2016 Accepted 22-12-2016 Available Online 30-12-2016 1 Celal Bayar University School of Medicine, Department of Biophysics, Manisa, TR 2 Yüzüncü Yıl University School of Medicine, Department of Pharmacology, Van, TR 3 Istanbul Medeniyet University, School of Medicine, Department of Nuclear Medicine, Istanbul, TR 4 Hacettepe University, Faculty of Engineering, Dept. of Nuclear Engineering, Ankara, TR 5 Celal Bayar University School of Medicine, Department of Urology, Manisa, TR * Corresponding Author: Zafer Akan E-mail: zaferakan@marmara.edu.tr Phone: +44 020 3289 9294 The treatment of metastatic prostate carcinoma with BNCT in the ITU TRIGA MARKII reactor on rat model