Gunasekaran Kannan
Amgen
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Publication
Featured researches published by Gunasekaran Kannan.
Molecular Immunology | 2013
Yaoqing Diana Liu; Tian Wang; Robert Y.-T. Chou; Louise Chen; Gunasekaran Kannan; Riki Stevenson; Andrew M. Goetze; Xinzhao Grace Jiang; Gang Huang; Thomas M. Dillon; Gregory C. Flynn
Human IgG2 antibodies contain three types of disulfide isoforms, classified by the number of Fab arms having disulfide links to the heavy chain hinge region. In the IgG2-B form, both Fab arms have interchain disulfide bonds to the hinge region, and in IgG2-A, neither Fab arm are disulfide linked to the hinge. The IgG2-A/B is a hybrid between these two forms, with only one Fab arm disulfide linked to the hinge. Changes in the relative levels of these forms over time are observed while IgG2 circulates in humans, suggesting IgG2-A→IgG2-A/B→IgG2-B conversion. Using a flow-through dialysis system, we studied the conversion kinetics of these forms in vitro under physiological conditions. For two IgG2κ antibodies, in vivo results closely matched the kinetics observed in vitro, indicating that the changes observed in vivo were solely conversions between isoforms, not differential clearance of specific forms. Moreover, the combined results validate the accuracy of the physiological model for the study of blood redox reactions. Further exploration of the conversion kinetics using material enriched in the IgG2-A forms revealed that the IgG2-A→IgG2-A/B rate was similar between IgG2κ and IgG2λ antibodies. In IgG2κ antibodies, conversion of IgG2-A/B→IgG2-B was slower than the IgG2-A→IgG2-A/B reaction. However, in IgG2λ antibodies, little IgG2-A/B→IgG2-B conversion was detected under physiological conditions. Thus, small differences in the C-terminus of the light chain sequences affect the disulfide conversion kinetics and impact the IgG2 disulfide isoforms produced in vivo.
Archive | 2009
Gunasekaran Kannan; Michael Wittekind; Wei Yan; Martin J. Pentony
Archive | 2010
Gunasekaran Kannan; Hongxing Zhou; Nancy Sun
Archive | 2010
Gunasekaran Kannan; Hongxing Zhou
Archive | 2013
Yumei Xiong; Yi Zhang; Jackie Zeqi Sheng; Agnes Eva Hamburger; Murielle Veniant-Ellison; Grant Shimamoto; Xiaoshan Min; Zhulun Wang; Jie Tang; Gunasekaran Kannan; Kenneth W. Walker; Bryan Lemon
Archive | 2014
Marc A. Gavin; Gunasekaran Kannan; Li Li; Joshua T. Pearson; Margaret Karow
Archive | 2012
Gunasekaran Kannan
Archive | 2014
Yumei Xiong; Yi Zhang; Jackie Zeqi Sheng; Agnes Eva Hamburger; Murielle Veniant-Ellison; Grant Shimamoto; Xiaoshan Min; Zhulun Wang; Jie Tang; Gunasekaran Kannan; Marissa Mock; Kenneth W. Walker
Archive | 2013
Richard J. Armitage; Michelle Blake; Iii William Christian Fanslow; Jason C. O'neill; Gunasekaran Kannan; Jiangchun Xu; Mark E. Tometsko; Zhulun Wang; Athena Sudom
Archive | 2014
Hailing Hsu; Ming Zhang; Gunasekaran Kannan; Frederick W. Jacobsen; Wayne Tsuji