Guo-hong Li
Southeast University
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Featured researches published by Guo-hong Li.
International Journal of Nanomedicine | 2009
Baoan Chen; Jian Cheng; Mingfang Shen; Feng Gao; Xu Wl; Hui-lin Shen; Ding Jh; Chong Gao; Qian Sun; Xin-chen Sun; Hongyan Cheng; Guo-hong Li; Wen-ji Chen; Ning-Na Chen; Li-jie Liu; Xiaomao Li; Xuemei Wang
Apoptosis is a common pathway that finally mediated the killing functions of anticancer drugs, which is an important cause of multidrug resistance (MDR). The aim of this study was to investigate the potential benefit of combination therapy with magnetic nanoparticle of Fe3O4 (MNP(Fe3O4)) and 5-bromotetrandrin (BrTet). Analysis of the apoptosis percentage showed that combination of daunorubicin (DNR) with either MNP(Fe3O4) or BrTet exerted a potent cytotoxic effect on K562/A02 cells, while MNP(Fe3O4) and BrTet cotreatment can synergistically enhance DNR-induced apoptosis. Importantly, we confirmed that the distinct synergism effect of that composite on reverse multidrug resistance may owe to the regulation of various proliferative and antiapoptotic gene products, including P53 and caspase-3. Thus our in vitro data strongly suggests a potential clinical application of MNP(Fe3O4) and BrTet combination on CML.
International Journal of Nanomedicine | 2009
Baoan Chen; Jian Cheng; Yanan Wu; Feng Gao; Xu Wl; Hui-lin Shen; Ding Jh; Chong Gao; Qian Sun; Xin-chen Sun; Hongyan Cheng; Guo-hong Li; Wen-ji Chen; Ning-Na Chen; Li-jie Liu; Xiaomao Li; Xuemei Wang
In this paper we establish the xenograft leukemia model with stable multidrug resistance in nude mice and to investigate the reversal effect of 5-bromotetrandrine (5-BrTet) and magnetic nanoparticle of Fe3O4 (MNP-Fe3O4) combined with daunorubicin (DNR) in vivo. Two subclones of K562 and K562/A02 cells were inoculated subcutaneously into the back of athymic nude mice (1 × 107 cells/each) respectively to establish leukemia xenograft models. Drug-resistant and sensitive tumor-bearing nude mice were assigned randomly into five groups which were treated with normal saline; DNR; NP-Fe3O4 combined with DNR; 5-BrTet combined with DNR; 5-BrTet and MNP-Fe3O4 combined with DNR, respectively. The incidence of formation, growth characteristics, weight, and volume of tumors were observed. The histopathologic examination of tumors and organs were detected. For resistant tumors, the protein levels of Bcl-2, and BAX were detected by Western blot. Bcl-2, BAX, and caspase-3 genes were also detected. For K562/A02 cells xenograft tumors, 5-BrTet and MNP-Fe3O4 combined with DNR significantly suppressed growth of tumor. A histopathologic examination of tumors clearly showed necrosis of the tumors. Application of 5-BrTet and MNP-Fe3O4 inhibited the expression of Bcl-2 protein and upregulated the expression of BAX and caspase-3 proteins in K562/A02 cells xenograft tumor. It is concluded that 5-BrTet and MNP-Fe3O4 combined with DNR had a significant tumor-suppressing effect on a MDR leukemia cells xenograft model.
International Journal of Nanomedicine | 2009
Baoan Chen; Yiqiong Liang; Weiwei Wu; Jian Cheng; Guohua Xia; Feng Gao; Ding Jh; Chong Gao; Shao Zy; Guo-hong Li; Wen-ji Chen; Xu Wl; Xin-chen Sun; Li-jie Liu; Xiaomao Li; Xuemei Wang
Gambogic acid (GA) has a significant anticancer effect on a wide variety of solid tumors. Recently, many nanoparticles have been introduced as drug-delivery systems to enhance the efficiency of anticancer drug delivery. The aim of this study was to investigate the potential benefit of combination therapy with GA and magnetic nanoparticles of Fe3O4 (MNPs-Fe3O4). The proliferation of K562 cells and their cytotoxicity were evaluated by MTT assay. Cell apoptosis was observed and analyzed by microscope and flow cytometry, respectively. Furthermore, real-time polymerase chain reaction and Western blotting analyses were performed to examine gene transcription and protein expression, respectively. The results showed that MNPs-Fe3O4 dramatically enhanced GA-induced cytotoxicity and apoptosis in K562 cells. The typical morphological features of apoptosis treated with GA and MNPs-Fe3O4 were observed under an optical microscope and a fluorescence microscope, respectively. The transcription of caspase-3 and bax gene in the group treated with GA and MNPs-Fe3O4 was higher than that in the GA-alone group or MNPs-Fe3O4-alone group, but the transcription of bcl-2, nuclear factor-κB, and survivin degraded as did the expression of corresponding proteins in K562 cells. Our data suggests a potential clinical application of a combination of GA and MNPs-Fe3O4 in leukemia therapy.
Chinese journal of cancer | 2002
Chen Tan; Cong Peng; Ying Huang; Zhang Qh; Tang K; Xiaomao Li; Guo-hong Li
Chinese journal of cancer | 2009
Zhi Jiang; Baoan Chen; Qiang Wu; Guohua Xia; Yu Zhang; Feng Gao; Tie-Yan Hong; Cui-Rong Xu; Jian Cheng; Guo-hong Li; Wen-ji Chen; Li-jie Liu; Xiaomao Li; Xuemei Wang
Chinese journal of cancer | 2002
Cong Peng; Zhou M; Zhang Qh; Tang K; Guo-hong Li
Journal of Experimental Hematology | 2009
Mingfang Shen; Bao-An Chen; Jian Cheng; Feng Gao; Xu Wl; Ding Jh; Chong Gao; Xin-chen Sun; Guo-hong Li; Wen-ji Chen; Li-jie Liu; Xiaomao Li; 王雪
Blood | 2008
Bao-An Chen; Bin-bin Lai; Jian Cheng; Feng Gao; Xu Wl; Hui-lin Shen; Ding Jh; Chong Gao; Yun-Yu Sun; Jun Wang; Gang Zhao; Song Hh; Wen Bao; Xin-chen Sun; Hongyan Cheng; Yu-xia Deng; Guo-hong Li; Li-jie Liu; Wen-ji Chen; Shao Zy; Guohua Xia; Jue-qiong Wang; Peng Yuan; Xiao-Ping Pei; Ning-Na Chen; Qin-ning Li; Xuemei Wang
Blood | 2008
Bao-An Chen; Mingfang Shen; Jian Cheng; Feng Gao; Xu Wl; Hui-lin Shen; Ding Jh; Chong Gao; Yun-Yu Sun; Jun Wang; Gang Zhao; Song Hh; Wen Bao; Xin-chen Sun; Hongyan Cheng; Yu-xia Deng; Guo-hong Li; Li-jie Liu; Wen-ji Chen; Jue-qiong Wang; Peng Yuan; Xiao-Ping Pei; Ning-Na Chen; Xuemei Wang
Blood | 2008
Bao-An Chen; Bin-bin Lai; Jian Cheng; Feng Gao; Xu Wl; Hui-lin Shen; Ding Jh; Chong Gao; Yun-Yu Sun; Jun Wang; Gang Zhao; Song Hh; Wen Bao; Xin-chen Sun; Hongyan Cheng; Yu-xia Deng; Guo-hong Li; Li-jie Liu; Wen-ji Chen; Shao Zy; Guohua Xia; Jue-qiong Wang; Peng Yuan; Xiao-Ping Pei; Ning-Na Chen; Qin-ning Li; Xuemei Wang