Gustavo Muniz Dias

Distinct community dynamics at two artificial habitats in a recreational marina

Man-made facilities along coastlines modify water circulation and sedimentation dynamics which can affect the structure of marine benthic and pelagic communities. To test how environmental heterogeneity associated with a recreational marina affects the structure of the fouling community and the benthic-pelagic link, we conducted an experiment in which predation effects on recruitment and community structure were assessed in two artificial habitats: inside the marina, an area of calm waters and often disturbed by boating activity, and the breakwater, a more hydrodynamic area. Using visual censuses and video footages we also described the predation pressure and the identity of predators on the two areas. Inside the marina, the recruitment of ascidians and serpulids, but not of bryozoans, was restricted in some occasions, possibly due to reduced water circulation. Predation, mainly by the silver porgy fish Diplodus argenteus, reduced the survivor of didemnid ascidians on both areas, but predation intensity was 40 times higher in the breakwater than inside the marina. While the two artificial habitats did not necessarily support distinct communities, low recruitment coupled to weak predation inside the marina, a less dynamic environment, likely imply lower resilience and more susceptibility to disturbance.

Current conditions and colonization history asymmetrically shape the organization of shallow sessile communities after simulated state shifts

Historical processes affecting biological organization are rarely considered when predicting the effects of disturbance on community structure. In order to assess the relative importance of historical and post-disturbance conditions as determinants of community structure, we undertook reciprocal transplants, at different successional stages, of sessile communities developing at recreational piers that were previously observed to show contrasting fish predation pressure and settlement rate in the São Sebastião Channel, Brazil. Regardless the direction of state shift, after 15 weeks communities converged to the destination site structure, substantially drifting away from the path observed at origin, therefore revealing high susceptibility to environmental change. Although converging, transplanted communities never matched the destination standard in both transplant directions, suggesting that history still mattered, as providing some legacy that lasted, at least, for 15 weeks. The taxonomic groups resisting community drift were hard-bodied invertebrates, which could eventually provide some resilience to these communities through ecosystem engineering.

CHEMICAL PROFILE AND CITOTOXIC POTENCIAL OF A MARINE-DERIVED FUNGUS Trichoderma harzianum

Purpose: The objective of this work will be to identify the nutritional and alimentary impact during the treatment of patients with head and neck neoplasm. Methods: The present study will be carried out in patients of the Hospital do Cancer de Franca-SP, with the authorization of the coordinator responsible for the hospital and later the Research Ethics Committee - CEPE of Santa Casa de Franca. The research will be developed through a retrospective analysis of medical records of patients presenting with head and neck neoplasm, male and female, over 18 years of age, from June 2017 to May 2018 at Hospital do Cancer de Franca. Firstly, medical records of all patients with head and neck cancer who are undergoing antineoplastic treatment will be evaluated, covering the methods of radiotherapy, chemotherapy and surgeries. The analysis will be performed to collect information on anthropometric, biochemical, clinical and dietary data of certain patients. The variables to be analyzed will receive descriptive statistical treatment through tables and frequency graphs and for variables of a quantitative nature, the calculation of descriptive parameters such as arithmetic mean, median, standard deviation and coefficient of variation will complete the descriptive process. Results: The worlds cancer incidence rate exceeds 14 million people per year, and by 2030 it is estimated that more than 21 million new cases in the world. The present work is in the final phase of data collection and the next step will be the treatment and discussion of the data.Purpose: To design, construct and validate a system with efficiency of electric and thermal energy generation through refrigerated photovoltaic panels (hybrids). The focus of this work will be to optimize the energy efficiency of the photovoltaic panel and consequently to obtain the thermal energy generation with the energy dissipated by the system. Methods: Through a literature review from renowned authors, books and scientific articles, as well as a research applied through the design, construction and validation of a prototype, the experimental data of the panels are being collected through appropriate instruments, as well as validation of the modeling through data analysis and comparison between the techniques of capturing solar energy and cooling of the panels that best corresponds to the efficiency of the project. Results: The photovoltaic panel loses efficiency when its temperature increases. Although we have advanced well in the project, until the writing of this summary we were still testing the refrigeration system that provides better efficiency. Conclusion: From the study and experiences that have been and will still be, it was expected that the refrigerated panel would provide a greater generation of electrical energy in relation to the non-refrigerated and consequently already heat the water, thus obtaining a better use of energy.Purpose: The Community Group of Mental Health is characterized by an intervention modality with the purpose of promoting mental health, the dynamics of this group construction develops in a welcoming environment through the interaction between person and daily life thus promoting personal and collective growth. Method: Therefore, in the context of this research, it is emphasized the relevance of the use of this modality of group intervention for the promotion of mental health in the psychologist’s academic development, taking into account the statistic’s increase of psychological vulnerability in the university environment. The researchs main goal is to collect and analyze productions in the area of community groups on mental health and the psychologists training in the period from 2012 to 2017, The researchs main goal is to collect and analyze productions in the area of community groups on mental health and the psychologists training in the period from 2012 to 2017, aiming to characterize the focus of these productions and their contributions in psychology training. Results: The methodology used was a descriptive-qualitative research, through the integrative review of the scientific productions in the area during the period from 2012 to 2017 in PUBmed, BVsalud, Scielo and CAPES electronic scientific journals, using as descriptors mental health in university students and community mental health groups. The analysis was created integrating the results of the productions on Community Mental Health Groups with surveys about the psychological suffering in the graduations context. Among the works found three are in English and five in Spanish. Conclusion: The scientific productions in the search made addressed themes related to psychological vulnerability in the university environment as well as on Community Groups on Mental Health.Objective: This research deals with the new extra-judicial usucapion procedure, introduced in the legal system by the New Civil Procedure Code (Law 13.015/15). In his art. 1,071, the New Civil Code added art. 216-A in the Law n.6.015/73 (Public Registers), and established, in its paragraphs, a list of legal requirements for acquisition of property (real state) by extrajudicial misappropriation. Methods: The research aims at the interpretation of the norms that make up the legal institute of extrajudicial usucapion; analyzes interrelated norms of equal or different hierarchies in the legal system, starting with the Federal Constitution; analyzes the infraconstitutional legislation and normative acts that regulate the extra-judicial usucapion; analyzes technical reports from notary public registries and court decisions, in order to understand the operational aspects of the legal institute researched. Results: The results are partial, with positive and negative aspects and some controversial points, since its application is still in the phase of adaptation by the notaries. There have been practical doubts, however, in view of this, on 14/12/2017, the National Council of Justice – CNJ – brought to public attention a document, which established several guidelines for the procedure of extrajudicial misappropriation in notary public registries and public real estate registries. Conclusion: The extrajudicial misappropriation procedure allowed the recognition by extrajudicial way of all the usucapion modalities foreseen in the Brazilian law, provided that all the legal requirements are met. Thus, the present study aimed to study the new procedure, which brought speed and quick resolution of the demands, but did not exclude the discretion of the judiciary, since the procedure is optional, so it will be up to the interested party to choose. Keywords: unfairness; procedure; usucapion extrajudicial. Acknowledgments: CAPES, CNPqPurpose: the aim of this study was to evaluate the in vitro efficacy of three differents decontaminating treatments on oral bacterial biofilms developed on the surface of pacifiers made with silicone or latex. Methods: on the surfaces of 96 pacifiers made of silicone and latex of two comercial brands, biofilms were developed from the following reference strains (ATCC): Streptococcus mutans (25175) and Lactobacillus casei (11578). The pacifiers were then divided into 4 groups (N=12) according to the treatments: control group (without treatment), boiling water (1 minute), microwave (7 minutes), and chlorhexidine digluconate solution at 0.12 % (spray 6 times, 0.6 mL). The data were submitted to one-way ANOVA for repeated measures. The significance level established was of 5%. Results: at the moment, the data are under statistical treatment and will be available for presentation at the event. Conclusions: the conclusions will be formulated from the results of the statistical analysis.Purpose: Total Quality Management (TQM) is based on the application of 7 principles of quality to cover all aspects of an organization and ensure the total quality of the product offered to the customer. This article had as objective to analyze the implementation of the seven principles of TQM in civil construction. Methods: A descriptive and explanatory case study was conducted. We interviewed the Quality Manager with the objective of understanding the techniques and actions of quality management in projects of a large construction company located in the interior of the State of Sao Paulo. Results: It was possible to highlight the importance of the involvement of each collaborator of the organization, the need for top management support and the influence that TQM exerts on decision making and the product delivered to the final customer. Conclusion: We conclude that the scope of quality management is associated to several aspects of an organization, and a good management and the degree of satisfactory involvement of its employees is necessary. The use of TQM provides total quality assurance and a positive outlook for continuous improvement, which benefits the maintenance of the companys competitiveness in the market.Purpose: Although the standardization of disinfection processes by national and international organizations, there is a contamination of dialysis machines caused by fungus, especially in the biofilm growth (microorganisms held together by exopolymeric substance and adhered to a surface), has increased patients risk of acquiring infection. In addition, concentration of nutrients in the ambient, salinity, pH and oxygenation modulate the biofilm formation. The anti-biofilm activity of peracetic acid, a disinfectant recently introduced for the disinfection of hemodialysis machines, against Fusarium biofilms was evaluated under two oxygenation conditions. The concentrations tested were 0.1% and 0.2%, with 30 minutes and 10 minutes exposure duration, respectively. Methods: The biofilms were formed in microaerophilic and aerophilic conditions in 96-well microplates at 30 ° C for 72 hours, determining their cell viability by the tetrazolium salt reduction assay (XTT). Results: The results showed that 0.2% peracetic acid during 10 minutes effectively reduced the metabolic activity of Fusarium biofilms (p<0.05). Comparative analysis between aerophilia and microaerophilia showed higher efficiency for the microaerophilic condition (p <0.001). Conclusion: The 0.2% peracetic acid with a 10 min-exposure time showed to be a potent agent for disinfection of the dialysis machines infected with Fusarium biofilms. Disinfection efficiency can contribute to a better quality of life for patients who require renal replacement therapy, as well as reduce the costs of the public health system, reducing hospitalization for infection.Introduction: Neglected tropical diseases, a diverse group of diseases that prevail in tropical and subtropical conditions in 149 countries. Schistosomiasis is the third most devastating tropical disease in the world, being a major source of morbidity and mortality for developing countries. The treatment and control of schistosomiasis virtually relies on a single drug, praziquantel. The pressing need to develop new antischistosomal compounds has been emphasized, particularly in view of blanket application of praziquantel within the frame of preventive chemotherapy, a strategy that might select for drug-resistant parasites. Purpose: Here, we report the in vitro effect of crude extract and fractions of Baccharis dracunculifolia green propolis on Schistosoma mansoni adult worms . Methods: Green propolis was subjected to exhaustive maceration with ethanol. The obtained ethanol solution was filtered, concentrated and then submitted to liquid/liquid partition using different solvents. The obtained fractions, as well as the crude ethanolic extract, were analyzed by HPLC/DAD and evaluated in schistosomicidal assays Results: The crude ethanolic extract of Baccharis dracunculifolia green propolis (at a concentration of 200 μg/mL) showed substancial results, killing 93.75% of the Schistosoma mansoni worms. Dichloromethane and hexane fractions showed the greatest schistosomicidal effect (93.75% and 87.5%, respectively) while ethyl acetate and butanol fractions did not show schistosomicidal activity at the same concentration evaluated. Conclusion: Based on chemical and biological evaluation, the two main fractions of green propolis were selected for further study of isolation and identification of compounds with schistosomicidal activity.Purpose: Stenoma catenifer (Lepidoptera: Elachistidae), avocado seed moth, is considered a key pest in avocado because of the direct damages caused to the fruits by the entrance of the larvae and the construction of galleries. Thus, the objective was to determine a methodology of laboratory rearing to obtain insects for research on it behavior, biology, ecology and mainly control. Methods: The beginning of the laboratory establishment was with insects collected in organic crop of avocado located in Franca, SP (20 ° 32’19’’S 47 ° 24’03’’W). The insects were taken to the Laboratory of Agricultural Entomology of Unifran and the material was screened to obtain the S. catenifer larvae. Results: The fruits containing larvae were packed in transparent plastic containers until the pupae were formed (36cm length, 25.5cm width and 12.5 height). The pupae were transferred to other transparent plastic containers (11cm length, 11cm width and 7.5cm height) containing a layer of vermiculite to fix the pupae. In the adult phase, couples were placed in mate and oviposition cages (14cm length, 14cm width and 22cm height), containing a fruit to stimulate oviposition (fixed in support) and two sponges under pet bottle caps to offer the diet (water and honey at 10%). The top of the cage was closed with voil fabric to promote aeration. Conclusion : The methodology used is suitable for mass rearing of avocado seed moth S. catenifer .Purpose : The increase in water intake has been studied as a method to decrease urine specific gravity in order to prevent urolithiasis, interstitial cystitis and feline urethral obstruction. This stud aims to understand if the type of drinking fountain will reflect in the increase of water intake and how this will influence the urine specific gravity and possible prevention of the diseases mentioned above. Methods: This study proposes to compare the amount of water ingested and which type of waterfall is preferred by felines through camera recording. Thirty healthy cats with a body weight of 3 to 6 kg (score 5 to 6) and range from 2 to 5 years. Results: To date, 22 animals have been evaluated, where partial results have shown that the feline normally has a preference for its usual source and new sources interfere in a negative way in its ingestion, in addition, it was observed that places where there is more than one animal, dominant feline is the one that ingests greater amount of water and prevents other felines from ingesting it, generating fear in the others. Conclusions: Thus, the implementation of new sources tends to be a disadvantage among felines and the dominance factor between them also interferes in these results.Purpose: The parasitoid Cotesia flavipes Cameron (Hymenoptera: Braconidae), used in the control of the sugarcane borer Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) is applied in about 3.5 million hectares in Brazil. Currently, 4 to 8 plastic containers are released manually, corresponding to about 6 thousand parasitoids per hectare. In this way, workers walk between the lines of culture to make the release. The objective of this work was to verify the longevity of adults in plastic and biodegradable packaging, trying to provide a packaging that is environmentally adequate, has rapid degradation in the field and allows the release of parasitoids using drone. Methods: As soon as the insects emerged, two pairs were individualized per pack, observing 10 repetitions for each package. The evaluations were carried out every 4 hours until the death of the insects that were kept under controlled conditions (temperature of 25 ± 1 °C and photoperiod of 12h of light/12h of dark) and remained without food. Results: The longevity of adults was influenced by the packages. The males showed longevity of 36.4 hours in the plastic packages and 25.5 hours in the biodegradable ones. The females showed longevity of 37.4 hours in the plastic packages and 27.0 hours in the biodegradable ones. Conclusion : The plastic packages provided greater longevity of the adults of the C. flavipes parasitoid. However, parasitoids can be released in the field using the biodegradable packages in drones if the release occurs soon after the emergence of adults.Objective: Once the three rhetorical fundamental pillars are stablished as ethos , pathos , and logos , the aim of this paper was to unveil the Aristotelian passions, based on the pathos sphere, in a musical-rhetorical corpus – i.e. the image-sound bond in a cinematographic piece. The corpus is composed by the sixth episode of the 2014 Argentinean movie, Wild Tales . Entitled Till Death do us Apart , the episode deflagrates the perfect bond between image and sound and how this relationship is powerful concerning the persuasion matter. Methods: In order to achieve this conclusion, we performed a rhetorical analysis based on both: Musical – Arakawa (1995), Bennett (1990), and Copland (1974) – and Rhetorical – Aristotle (2000), Meyer (2007), Reboul (2004), Ferreira (2010), Abreu (2001), and Fiorin (2014) – theories. The objective was to consider how the image-sound bond could evoke and project the Aristotelian passions from the plot to the audience, turning this process into persuasion. Results: The preliminary results showed us that the image-sound bond, in a cinematographic sphere, can be used as a bridge to awaken emotions, intended by the plot, in the audience, turning the movie meanings more tangible. Moreover, this bond can also create new nuances, bringing new meanings to the piece. Through our rhetorical analysis it was possible to deflagrate the power of Music concerning Rhetoric and persuasion in a cinematographic piece.

CHEMICAL PROFILE AND SCHISTOSOMICIDAL INVESTIGATION OF A MARINE-DERIVED FUNGUS Penicillium citrinum

Purpose: The objective of this work will be to identify the nutritional and alimentary impact during the treatment of patients with head and neck neoplasm. Methods: The present study will be carried out in patients of the Hospital do Cancer de Franca-SP, with the authorization of the coordinator responsible for the hospital and later the Research Ethics Committee - CEPE of Santa Casa de Franca. The research will be developed through a retrospective analysis of medical records of patients presenting with head and neck neoplasm, male and female, over 18 years of age, from June 2017 to May 2018 at Hospital do Cancer de Franca. Firstly, medical records of all patients with head and neck cancer who are undergoing antineoplastic treatment will be evaluated, covering the methods of radiotherapy, chemotherapy and surgeries. The analysis will be performed to collect information on anthropometric, biochemical, clinical and dietary data of certain patients. The variables to be analyzed will receive descriptive statistical treatment through tables and frequency graphs and for variables of a quantitative nature, the calculation of descriptive parameters such as arithmetic mean, median, standard deviation and coefficient of variation will complete the descriptive process. Results: The worlds cancer incidence rate exceeds 14 million people per year, and by 2030 it is estimated that more than 21 million new cases in the world. The present work is in the final phase of data collection and the next step will be the treatment and discussion of the data.Purpose: To design, construct and validate a system with efficiency of electric and thermal energy generation through refrigerated photovoltaic panels (hybrids). The focus of this work will be to optimize the energy efficiency of the photovoltaic panel and consequently to obtain the thermal energy generation with the energy dissipated by the system. Methods: Through a literature review from renowned authors, books and scientific articles, as well as a research applied through the design, construction and validation of a prototype, the experimental data of the panels are being collected through appropriate instruments, as well as validation of the modeling through data analysis and comparison between the techniques of capturing solar energy and cooling of the panels that best corresponds to the efficiency of the project. Results: The photovoltaic panel loses efficiency when its temperature increases. Although we have advanced well in the project, until the writing of this summary we were still testing the refrigeration system that provides better efficiency. Conclusion: From the study and experiences that have been and will still be, it was expected that the refrigerated panel would provide a greater generation of electrical energy in relation to the non-refrigerated and consequently already heat the water, thus obtaining a better use of energy.Purpose: The Community Group of Mental Health is characterized by an intervention modality with the purpose of promoting mental health, the dynamics of this group construction develops in a welcoming environment through the interaction between person and daily life thus promoting personal and collective growth. Method: Therefore, in the context of this research, it is emphasized the relevance of the use of this modality of group intervention for the promotion of mental health in the psychologist’s academic development, taking into account the statistic’s increase of psychological vulnerability in the university environment. The researchs main goal is to collect and analyze productions in the area of community groups on mental health and the psychologists training in the period from 2012 to 2017, The researchs main goal is to collect and analyze productions in the area of community groups on mental health and the psychologists training in the period from 2012 to 2017, aiming to characterize the focus of these productions and their contributions in psychology training. Results: The methodology used was a descriptive-qualitative research, through the integrative review of the scientific productions in the area during the period from 2012 to 2017 in PUBmed, BVsalud, Scielo and CAPES electronic scientific journals, using as descriptors mental health in university students and community mental health groups. The analysis was created integrating the results of the productions on Community Mental Health Groups with surveys about the psychological suffering in the graduations context. Among the works found three are in English and five in Spanish. Conclusion: The scientific productions in the search made addressed themes related to psychological vulnerability in the university environment as well as on Community Groups on Mental Health.Objective: This research deals with the new extra-judicial usucapion procedure, introduced in the legal system by the New Civil Procedure Code (Law 13.015/15). In his art. 1,071, the New Civil Code added art. 216-A in the Law n.6.015/73 (Public Registers), and established, in its paragraphs, a list of legal requirements for acquisition of property (real state) by extrajudicial misappropriation. Methods: The research aims at the interpretation of the norms that make up the legal institute of extrajudicial usucapion; analyzes interrelated norms of equal or different hierarchies in the legal system, starting with the Federal Constitution; analyzes the infraconstitutional legislation and normative acts that regulate the extra-judicial usucapion; analyzes technical reports from notary public registries and court decisions, in order to understand the operational aspects of the legal institute researched. Results: The results are partial, with positive and negative aspects and some controversial points, since its application is still in the phase of adaptation by the notaries. There have been practical doubts, however, in view of this, on 14/12/2017, the National Council of Justice – CNJ – brought to public attention a document, which established several guidelines for the procedure of extrajudicial misappropriation in notary public registries and public real estate registries. Conclusion: The extrajudicial misappropriation procedure allowed the recognition by extrajudicial way of all the usucapion modalities foreseen in the Brazilian law, provided that all the legal requirements are met. Thus, the present study aimed to study the new procedure, which brought speed and quick resolution of the demands, but did not exclude the discretion of the judiciary, since the procedure is optional, so it will be up to the interested party to choose. Keywords: unfairness; procedure; usucapion extrajudicial. Acknowledgments: CAPES, CNPqPurpose: the aim of this study was to evaluate the in vitro efficacy of three differents decontaminating treatments on oral bacterial biofilms developed on the surface of pacifiers made with silicone or latex. Methods: on the surfaces of 96 pacifiers made of silicone and latex of two comercial brands, biofilms were developed from the following reference strains (ATCC): Streptococcus mutans (25175) and Lactobacillus casei (11578). The pacifiers were then divided into 4 groups (N=12) according to the treatments: control group (without treatment), boiling water (1 minute), microwave (7 minutes), and chlorhexidine digluconate solution at 0.12 % (spray 6 times, 0.6 mL). The data were submitted to one-way ANOVA for repeated measures. The significance level established was of 5%. Results: at the moment, the data are under statistical treatment and will be available for presentation at the event. Conclusions: the conclusions will be formulated from the results of the statistical analysis.Purpose: Total Quality Management (TQM) is based on the application of 7 principles of quality to cover all aspects of an organization and ensure the total quality of the product offered to the customer. This article had as objective to analyze the implementation of the seven principles of TQM in civil construction. Methods: A descriptive and explanatory case study was conducted. We interviewed the Quality Manager with the objective of understanding the techniques and actions of quality management in projects of a large construction company located in the interior of the State of Sao Paulo. Results: It was possible to highlight the importance of the involvement of each collaborator of the organization, the need for top management support and the influence that TQM exerts on decision making and the product delivered to the final customer. Conclusion: We conclude that the scope of quality management is associated to several aspects of an organization, and a good management and the degree of satisfactory involvement of its employees is necessary. The use of TQM provides total quality assurance and a positive outlook for continuous improvement, which benefits the maintenance of the companys competitiveness in the market.Purpose: Although the standardization of disinfection processes by national and international organizations, there is a contamination of dialysis machines caused by fungus, especially in the biofilm growth (microorganisms held together by exopolymeric substance and adhered to a surface), has increased patients risk of acquiring infection. In addition, concentration of nutrients in the ambient, salinity, pH and oxygenation modulate the biofilm formation. The anti-biofilm activity of peracetic acid, a disinfectant recently introduced for the disinfection of hemodialysis machines, against Fusarium biofilms was evaluated under two oxygenation conditions. The concentrations tested were 0.1% and 0.2%, with 30 minutes and 10 minutes exposure duration, respectively. Methods: The biofilms were formed in microaerophilic and aerophilic conditions in 96-well microplates at 30 ° C for 72 hours, determining their cell viability by the tetrazolium salt reduction assay (XTT). Results: The results showed that 0.2% peracetic acid during 10 minutes effectively reduced the metabolic activity of Fusarium biofilms (p<0.05). Comparative analysis between aerophilia and microaerophilia showed higher efficiency for the microaerophilic condition (p <0.001). Conclusion: The 0.2% peracetic acid with a 10 min-exposure time showed to be a potent agent for disinfection of the dialysis machines infected with Fusarium biofilms. Disinfection efficiency can contribute to a better quality of life for patients who require renal replacement therapy, as well as reduce the costs of the public health system, reducing hospitalization for infection.Introduction: Neglected tropical diseases, a diverse group of diseases that prevail in tropical and subtropical conditions in 149 countries. Schistosomiasis is the third most devastating tropical disease in the world, being a major source of morbidity and mortality for developing countries. The treatment and control of schistosomiasis virtually relies on a single drug, praziquantel. The pressing need to develop new antischistosomal compounds has been emphasized, particularly in view of blanket application of praziquantel within the frame of preventive chemotherapy, a strategy that might select for drug-resistant parasites. Purpose: Here, we report the in vitro effect of crude extract and fractions of Baccharis dracunculifolia green propolis on Schistosoma mansoni adult worms . Methods: Green propolis was subjected to exhaustive maceration with ethanol. The obtained ethanol solution was filtered, concentrated and then submitted to liquid/liquid partition using different solvents. The obtained fractions, as well as the crude ethanolic extract, were analyzed by HPLC/DAD and evaluated in schistosomicidal assays Results: The crude ethanolic extract of Baccharis dracunculifolia green propolis (at a concentration of 200 μg/mL) showed substancial results, killing 93.75% of the Schistosoma mansoni worms. Dichloromethane and hexane fractions showed the greatest schistosomicidal effect (93.75% and 87.5%, respectively) while ethyl acetate and butanol fractions did not show schistosomicidal activity at the same concentration evaluated. Conclusion: Based on chemical and biological evaluation, the two main fractions of green propolis were selected for further study of isolation and identification of compounds with schistosomicidal activity.Purpose: Stenoma catenifer (Lepidoptera: Elachistidae), avocado seed moth, is considered a key pest in avocado because of the direct damages caused to the fruits by the entrance of the larvae and the construction of galleries. Thus, the objective was to determine a methodology of laboratory rearing to obtain insects for research on it behavior, biology, ecology and mainly control. Methods: The beginning of the laboratory establishment was with insects collected in organic crop of avocado located in Franca, SP (20 ° 32’19’’S 47 ° 24’03’’W). The insects were taken to the Laboratory of Agricultural Entomology of Unifran and the material was screened to obtain the S. catenifer larvae. Results: The fruits containing larvae were packed in transparent plastic containers until the pupae were formed (36cm length, 25.5cm width and 12.5 height). The pupae were transferred to other transparent plastic containers (11cm length, 11cm width and 7.5cm height) containing a layer of vermiculite to fix the pupae. In the adult phase, couples were placed in mate and oviposition cages (14cm length, 14cm width and 22cm height), containing a fruit to stimulate oviposition (fixed in support) and two sponges under pet bottle caps to offer the diet (water and honey at 10%). The top of the cage was closed with voil fabric to promote aeration. Conclusion : The methodology used is suitable for mass rearing of avocado seed moth S. catenifer .Purpose : The increase in water intake has been studied as a method to decrease urine specific gravity in order to prevent urolithiasis, interstitial cystitis and feline urethral obstruction. This stud aims to understand if the type of drinking fountain will reflect in the increase of water intake and how this will influence the urine specific gravity and possible prevention of the diseases mentioned above. Methods: This study proposes to compare the amount of water ingested and which type of waterfall is preferred by felines through camera recording. Thirty healthy cats with a body weight of 3 to 6 kg (score 5 to 6) and range from 2 to 5 years. Results: To date, 22 animals have been evaluated, where partial results have shown that the feline normally has a preference for its usual source and new sources interfere in a negative way in its ingestion, in addition, it was observed that places where there is more than one animal, dominant feline is the one that ingests greater amount of water and prevents other felines from ingesting it, generating fear in the others. Conclusions: Thus, the implementation of new sources tends to be a disadvantage among felines and the dominance factor between them also interferes in these results.Purpose: The parasitoid Cotesia flavipes Cameron (Hymenoptera: Braconidae), used in the control of the sugarcane borer Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) is applied in about 3.5 million hectares in Brazil. Currently, 4 to 8 plastic containers are released manually, corresponding to about 6 thousand parasitoids per hectare. In this way, workers walk between the lines of culture to make the release. The objective of this work was to verify the longevity of adults in plastic and biodegradable packaging, trying to provide a packaging that is environmentally adequate, has rapid degradation in the field and allows the release of parasitoids using drone. Methods: As soon as the insects emerged, two pairs were individualized per pack, observing 10 repetitions for each package. The evaluations were carried out every 4 hours until the death of the insects that were kept under controlled conditions (temperature of 25 ± 1 °C and photoperiod of 12h of light/12h of dark) and remained without food. Results: The longevity of adults was influenced by the packages. The males showed longevity of 36.4 hours in the plastic packages and 25.5 hours in the biodegradable ones. The females showed longevity of 37.4 hours in the plastic packages and 27.0 hours in the biodegradable ones. Conclusion : The plastic packages provided greater longevity of the adults of the C. flavipes parasitoid. However, parasitoids can be released in the field using the biodegradable packages in drones if the release occurs soon after the emergence of adults.Objective: Once the three rhetorical fundamental pillars are stablished as ethos , pathos , and logos , the aim of this paper was to unveil the Aristotelian passions, based on the pathos sphere, in a musical-rhetorical corpus – i.e. the image-sound bond in a cinematographic piece. The corpus is composed by the sixth episode of the 2014 Argentinean movie, Wild Tales . Entitled Till Death do us Apart , the episode deflagrates the perfect bond between image and sound and how this relationship is powerful concerning the persuasion matter. Methods: In order to achieve this conclusion, we performed a rhetorical analysis based on both: Musical – Arakawa (1995), Bennett (1990), and Copland (1974) – and Rhetorical – Aristotle (2000), Meyer (2007), Reboul (2004), Ferreira (2010), Abreu (2001), and Fiorin (2014) – theories. The objective was to consider how the image-sound bond could evoke and project the Aristotelian passions from the plot to the audience, turning this process into persuasion. Results: The preliminary results showed us that the image-sound bond, in a cinematographic sphere, can be used as a bridge to awaken emotions, intended by the plot, in the audience, turning the movie meanings more tangible. Moreover, this bond can also create new nuances, bringing new meanings to the piece. Through our rhetorical analysis it was possible to deflagrate the power of Music concerning Rhetoric and persuasion in a cinematographic piece.

Environmental heterogeneity at small spatial scales affects population and community dynamics on intertidal rocky shores of a threatened bay system

Abstract Coastal areas are critical transition zones between marine and terrestrial domains that support a large diversity of species but are exposed to significant anthropogenic impacts. While most conservation and management strategies rely on understanding the drivers of diversity over large scales, the importance of small-scale variation in environmental conditions can be overlooked. Here we combined a descriptive and experimental approach to describe the spatio-temporal dynamics of sessile communities and of the population of the barnacle Chthamalus bisinuatus within Araca Bay, a transition zone in southeastern Brazil threatened by the expansion of the Sao Sebastiao Port. We compared the relative coverage of sessile organisms from rocky shores for 20 months and several population parameters of barnacles in three distinct regions of Araca Bay, a few hundred meters apart: the inner portion, close to the Mae Isabel River and the Port, the middle of the Bay, and the outer region, close to the bay entrance. Community structure varied significantly over these small spatial scales; the macroalga Ulva lactuca and the oyster Crassostrea sp. were more abundant in the inner portion of the Bay, where the influence of freshwater and organic pollution from Mae Isabel River is more intense. Recruitment and density of C. bisinuatus was higher on the shores close to the mouth of the Bay, but individual size increased as one moves into the bay. Experimental manipulation of barnacles originally recruiting at the outer part of the bay and translocated to both outer and inner positions showed that while recruitment is restricted at the inner region, barnacle growth and survival are higher than at the outer shore. Our results suggest that the gradient of physical conditions created along the Araca Bay can result in distinct dynamics of communities and populations and that the region close to the mouth of the bay is more dynamic, with a larger turnover of space. In the opposite direction, the inner part of the bay, which is closer to the Sao Sebastiao Port, is more prone to be disturbed by any large human modification on the coast as populations are less dynamic and communities tend to be monopolized by species associated with organic pollution. Besides providing useful data for the management of Araca Bay, our results also show that small-scale variation of environmental conditions can contribute to create distinct dynamics of populations and communities and should be addressed in assessments of environmental impacts on the coast.

A century of introductions by coastal sessile marine invertebrates in Angola, South East Atlantic Ocean

South Atlantic studies referring to non-native taxa are mostly restricted to Argentinean, Brazilian, and South African coasts. In this study we examined the literature to provide a list of sessile marine invertebrates along the Angolan coast, to infer its introduction status according to their biogeographical distribution and natural history. We reported 29 non-native and 7 cryptogenic species, a small number when compared to other South Atlantic regions of similar extension. Half of the non-native species were reported for Luanda. The majority of the introduced species had a northern hemisphere origin, a consequence of the main introduction route being from the North Atlantic/Mediterranean Sea during the Portuguese colonization. This is the first comprehensive assessment of this kind for the Angolan coast and the diversity of introduced species is certainly underestimated. Regular and rigorous assessments and monitoring of introduced marine species will help to understand the vectors, routes and time of introductions.

The importance of shore height and host identity for amphipod assemblages

ABSTRACT The spatial distribution of organisms associated with marine intertidal macroalgae may be a direct result of their tolerance to air exposure or an indirect consequence of the distribution of their host. We compared amphipod assemblages from five intertidal macroalgae to investigate their relationship with algal identity. To test the effect of height regardless of algal characteristics, we transplanted coralline algal turfs to three different levels within the intertidal zone and compared amphipod assemblages after 1 and 14 days. Interstitial volume was positively correlated to the abundance of amphipods, suggesting that this attribute may correspond better to the potential space for their occupation when compared to algal biomass, thallus volume or the ratio between thallus and interstitial volume. Algal level determined the structure of the amphipod assemblages. Upper-level (Acanthophora spicifera and Caulerpa racemosa) and intermediate-level (coralline) algae host similar amphipod assemblages dominated by Apohyale media, but different from lower-level algae (Padina gymnospora and Sargassum cymosum), which were dominated by Hyale niger. Ten of the 15 amphipod species reported from natural communities were found in the transplanted plots. Distinct pools of amphipod species colonized coralline transplants at upper and lower levels after 1 day. However, regardless of the position on the shore, transplanted coralline turfs supported similar assemblages after 14 days, indicating that algal identity is also important for species assemblages. Our results suggest that both height on the shore and host identity combine to determine the vertical structure of amphipod assemblages in the rocky intertidal.

CLAVEMINOLS FROM THE ASCIDIAN Clavelina oblonga

Purpose: The Brazilian sea constitutes a vast biome that remains to be investigated. The ascidian Clavelina oblonga (Clavelinidae) is widely distributed in the Sao Sebastiao Channel, Sao Paulo, Brazil with rare chemical data described in the literature . The aim of this research was to evaluate the chemical profile and the cytotoxity potential of C. oblonga . Methods: The methanolic extract of C. oblonga (CO) was cyclic loaded in a HP20 column eluted with increasing amounts of Me 2 CO in H 2 O (20% -100%), affording the fraction CO-16 B to CO-16F. The cytotoxicity test with each fraction was evaluated using the in vitro toxicology colorimetric assay – Kit XTT in a normal line of human lung fibroblast cells GM0792A. Results: All samples were analysed by TLC, HPLC and 1 H-NMR showing different chemical profiles. The fraction CO-16 D was the most active and altered the cellular viability of normal human fibroblast cells GM07492A with IC 50 of 45,8 11,0 ± 11,0 compared to the cells control . Conclusion: The marine environment is considered a promising source of structurally novel and pharmacologically interesting natural compounds but the chemical constitution and biological potential of C.oblonga remains unknown, for this reason, further studies are underway in order to purify the marine natural compounds from the active fractions.

CHEMICAL STUDY AND ANTIMICROBIAL AND CYTOTOXIC ACTIVITIES OF THE ASCIDIAN Clavelina oblonga

Purpose: Marine organisms produce a variety of structurally novel and pharmacologically interesting compounds. In this work the ascidian Clavelina oblonga (Clavelinidae) was selected for study. Methods: After collection (previously authorized by the Ministry of Environment -MMA n. 34958-2), the fresh material was extracted in methanol by maceration to obtain the respective crude extract (CO) which was analyzed by HPLC-DAD and 1 H-NMR and then subjected to the purification by cyclic column using the PSDVB polymer stationary phase to give the fractions CO-A to CO-F. The mobile phase used was mixtures of water and acetone (20% -100%). The obtained fractions were analyzed by TLC and HPLC-DAD and also submitted to antimicrobial and cytotoxic assays. Results: The chromatographic procedure performed with CO extract afforded fractions with different chemical profile. In the antimicrobial assay, the CO-D fraction was the most promising, showing inhibitory action against the bacteria Streptococcus mitis at a concentration of 50 µg/mL and also inhibited the strains Streptococcus bacteria (ATCC 10556 and clinical isolate) and Streptococcus oralis (clinical isolate) at the concentration of 100 μg/ml. Despite the cytotoxic assay, the CO-D fraction showed cytotoxicity to normal human fibroblast cells GM07492A with IC 50 of 45.8 ± 11.0 µg/mL. Conclusion: These results will lead the sequence of chemical research looking for the active compounds of C. oblonga.