Kátia Aparecida de Siqueira

Effect of Endophytic Fungal Associations on the Chemical Profile of in vitroVochysia divergens Seedlings

Vochysia divergens (Vochysiaceae) is considered an invasive species in the wetlands of the Brazilian Pantanal, which hinders the cultivation of agricultural species. In this study, we evaluated the chemical profile by HPLC-DAD (high-performance liquid chromatography-diode array detector) of leaves extracts from V. divergens seedlings inoculated with endophytic fungi isolated from V. divergens roots. These fungi were collected on dry (D) and wet (W) seasons in the Pantanal. The presence of tannin hexahydroxydiphenoyl (HHDP)-galloyl-glucose and flavone 3’,5’-dimethoxy-luteolin were predominant in the seedlings inoculated with endophytic fungi W experiments at 100 and 80%, respectively. Likewise, flavone 3’,5-dimethoxy-luteolin7-O-β-glucoside showed a similar representation in the two evaluated periods, compared with 5-methoxy-luteolin, which was detected only in seedlings inoculated with W endophytic fungi. This approach is new to V. divergens, which has no scientific data on its in vitro elicitation, in the search for a better understanding of the ecological relationships of this species.

CHEMICAL PROFILE AND CITOTOXIC POTENCIAL OF A MARINE-DERIVED FUNGUS Trichoderma harzianum

Purpose: The objective of this work will be to identify the nutritional and alimentary impact during the treatment of patients with head and neck neoplasm. Methods: The present study will be carried out in patients of the Hospital do Cancer de Franca-SP, with the authorization of the coordinator responsible for the hospital and later the Research Ethics Committee - CEPE of Santa Casa de Franca. The research will be developed through a retrospective analysis of medical records of patients presenting with head and neck neoplasm, male and female, over 18 years of age, from June 2017 to May 2018 at Hospital do Cancer de Franca. Firstly, medical records of all patients with head and neck cancer who are undergoing antineoplastic treatment will be evaluated, covering the methods of radiotherapy, chemotherapy and surgeries. The analysis will be performed to collect information on anthropometric, biochemical, clinical and dietary data of certain patients. The variables to be analyzed will receive descriptive statistical treatment through tables and frequency graphs and for variables of a quantitative nature, the calculation of descriptive parameters such as arithmetic mean, median, standard deviation and coefficient of variation will complete the descriptive process. Results: The worlds cancer incidence rate exceeds 14 million people per year, and by 2030 it is estimated that more than 21 million new cases in the world. The present work is in the final phase of data collection and the next step will be the treatment and discussion of the data.Purpose: To design, construct and validate a system with efficiency of electric and thermal energy generation through refrigerated photovoltaic panels (hybrids). The focus of this work will be to optimize the energy efficiency of the photovoltaic panel and consequently to obtain the thermal energy generation with the energy dissipated by the system. Methods: Through a literature review from renowned authors, books and scientific articles, as well as a research applied through the design, construction and validation of a prototype, the experimental data of the panels are being collected through appropriate instruments, as well as validation of the modeling through data analysis and comparison between the techniques of capturing solar energy and cooling of the panels that best corresponds to the efficiency of the project. Results: The photovoltaic panel loses efficiency when its temperature increases. Although we have advanced well in the project, until the writing of this summary we were still testing the refrigeration system that provides better efficiency. Conclusion: From the study and experiences that have been and will still be, it was expected that the refrigerated panel would provide a greater generation of electrical energy in relation to the non-refrigerated and consequently already heat the water, thus obtaining a better use of energy.Purpose: The Community Group of Mental Health is characterized by an intervention modality with the purpose of promoting mental health, the dynamics of this group construction develops in a welcoming environment through the interaction between person and daily life thus promoting personal and collective growth. Method: Therefore, in the context of this research, it is emphasized the relevance of the use of this modality of group intervention for the promotion of mental health in the psychologist’s academic development, taking into account the statistic’s increase of psychological vulnerability in the university environment. The researchs main goal is to collect and analyze productions in the area of community groups on mental health and the psychologists training in the period from 2012 to 2017, The researchs main goal is to collect and analyze productions in the area of community groups on mental health and the psychologists training in the period from 2012 to 2017, aiming to characterize the focus of these productions and their contributions in psychology training. Results: The methodology used was a descriptive-qualitative research, through the integrative review of the scientific productions in the area during the period from 2012 to 2017 in PUBmed, BVsalud, Scielo and CAPES electronic scientific journals, using as descriptors mental health in university students and community mental health groups. The analysis was created integrating the results of the productions on Community Mental Health Groups with surveys about the psychological suffering in the graduations context. Among the works found three are in English and five in Spanish. Conclusion: The scientific productions in the search made addressed themes related to psychological vulnerability in the university environment as well as on Community Groups on Mental Health.Objective: This research deals with the new extra-judicial usucapion procedure, introduced in the legal system by the New Civil Procedure Code (Law 13.015/15). In his art. 1,071, the New Civil Code added art. 216-A in the Law n.6.015/73 (Public Registers), and established, in its paragraphs, a list of legal requirements for acquisition of property (real state) by extrajudicial misappropriation. Methods: The research aims at the interpretation of the norms that make up the legal institute of extrajudicial usucapion; analyzes interrelated norms of equal or different hierarchies in the legal system, starting with the Federal Constitution; analyzes the infraconstitutional legislation and normative acts that regulate the extra-judicial usucapion; analyzes technical reports from notary public registries and court decisions, in order to understand the operational aspects of the legal institute researched. Results: The results are partial, with positive and negative aspects and some controversial points, since its application is still in the phase of adaptation by the notaries. There have been practical doubts, however, in view of this, on 14/12/2017, the National Council of Justice – CNJ – brought to public attention a document, which established several guidelines for the procedure of extrajudicial misappropriation in notary public registries and public real estate registries. Conclusion: The extrajudicial misappropriation procedure allowed the recognition by extrajudicial way of all the usucapion modalities foreseen in the Brazilian law, provided that all the legal requirements are met. Thus, the present study aimed to study the new procedure, which brought speed and quick resolution of the demands, but did not exclude the discretion of the judiciary, since the procedure is optional, so it will be up to the interested party to choose. Keywords: unfairness; procedure; usucapion extrajudicial. Acknowledgments: CAPES, CNPqPurpose: the aim of this study was to evaluate the in vitro efficacy of three differents decontaminating treatments on oral bacterial biofilms developed on the surface of pacifiers made with silicone or latex. Methods: on the surfaces of 96 pacifiers made of silicone and latex of two comercial brands, biofilms were developed from the following reference strains (ATCC): Streptococcus mutans (25175) and Lactobacillus casei (11578). The pacifiers were then divided into 4 groups (N=12) according to the treatments: control group (without treatment), boiling water (1 minute), microwave (7 minutes), and chlorhexidine digluconate solution at 0.12 % (spray 6 times, 0.6 mL). The data were submitted to one-way ANOVA for repeated measures. The significance level established was of 5%. Results: at the moment, the data are under statistical treatment and will be available for presentation at the event. Conclusions: the conclusions will be formulated from the results of the statistical analysis.Purpose: Total Quality Management (TQM) is based on the application of 7 principles of quality to cover all aspects of an organization and ensure the total quality of the product offered to the customer. This article had as objective to analyze the implementation of the seven principles of TQM in civil construction. Methods: A descriptive and explanatory case study was conducted. We interviewed the Quality Manager with the objective of understanding the techniques and actions of quality management in projects of a large construction company located in the interior of the State of Sao Paulo. Results: It was possible to highlight the importance of the involvement of each collaborator of the organization, the need for top management support and the influence that TQM exerts on decision making and the product delivered to the final customer. Conclusion: We conclude that the scope of quality management is associated to several aspects of an organization, and a good management and the degree of satisfactory involvement of its employees is necessary. The use of TQM provides total quality assurance and a positive outlook for continuous improvement, which benefits the maintenance of the companys competitiveness in the market.Purpose: Although the standardization of disinfection processes by national and international organizations, there is a contamination of dialysis machines caused by fungus, especially in the biofilm growth (microorganisms held together by exopolymeric substance and adhered to a surface), has increased patients risk of acquiring infection. In addition, concentration of nutrients in the ambient, salinity, pH and oxygenation modulate the biofilm formation. The anti-biofilm activity of peracetic acid, a disinfectant recently introduced for the disinfection of hemodialysis machines, against Fusarium biofilms was evaluated under two oxygenation conditions. The concentrations tested were 0.1% and 0.2%, with 30 minutes and 10 minutes exposure duration, respectively. Methods: The biofilms were formed in microaerophilic and aerophilic conditions in 96-well microplates at 30 ° C for 72 hours, determining their cell viability by the tetrazolium salt reduction assay (XTT). Results: The results showed that 0.2% peracetic acid during 10 minutes effectively reduced the metabolic activity of Fusarium biofilms (p<0.05). Comparative analysis between aerophilia and microaerophilia showed higher efficiency for the microaerophilic condition (p <0.001). Conclusion: The 0.2% peracetic acid with a 10 min-exposure time showed to be a potent agent for disinfection of the dialysis machines infected with Fusarium biofilms. Disinfection efficiency can contribute to a better quality of life for patients who require renal replacement therapy, as well as reduce the costs of the public health system, reducing hospitalization for infection.Introduction: Neglected tropical diseases, a diverse group of diseases that prevail in tropical and subtropical conditions in 149 countries. Schistosomiasis is the third most devastating tropical disease in the world, being a major source of morbidity and mortality for developing countries. The treatment and control of schistosomiasis virtually relies on a single drug, praziquantel. The pressing need to develop new antischistosomal compounds has been emphasized, particularly in view of blanket application of praziquantel within the frame of preventive chemotherapy, a strategy that might select for drug-resistant parasites. Purpose: Here, we report the in vitro effect of crude extract and fractions of Baccharis dracunculifolia green propolis on Schistosoma mansoni adult worms . Methods: Green propolis was subjected to exhaustive maceration with ethanol. The obtained ethanol solution was filtered, concentrated and then submitted to liquid/liquid partition using different solvents. The obtained fractions, as well as the crude ethanolic extract, were analyzed by HPLC/DAD and evaluated in schistosomicidal assays Results: The crude ethanolic extract of Baccharis dracunculifolia green propolis (at a concentration of 200 μg/mL) showed substancial results, killing 93.75% of the Schistosoma mansoni worms. Dichloromethane and hexane fractions showed the greatest schistosomicidal effect (93.75% and 87.5%, respectively) while ethyl acetate and butanol fractions did not show schistosomicidal activity at the same concentration evaluated. Conclusion: Based on chemical and biological evaluation, the two main fractions of green propolis were selected for further study of isolation and identification of compounds with schistosomicidal activity.Purpose: Stenoma catenifer (Lepidoptera: Elachistidae), avocado seed moth, is considered a key pest in avocado because of the direct damages caused to the fruits by the entrance of the larvae and the construction of galleries. Thus, the objective was to determine a methodology of laboratory rearing to obtain insects for research on it behavior, biology, ecology and mainly control. Methods: The beginning of the laboratory establishment was with insects collected in organic crop of avocado located in Franca, SP (20 ° 32’19’’S 47 ° 24’03’’W). The insects were taken to the Laboratory of Agricultural Entomology of Unifran and the material was screened to obtain the S. catenifer larvae. Results: The fruits containing larvae were packed in transparent plastic containers until the pupae were formed (36cm length, 25.5cm width and 12.5 height). The pupae were transferred to other transparent plastic containers (11cm length, 11cm width and 7.5cm height) containing a layer of vermiculite to fix the pupae. In the adult phase, couples were placed in mate and oviposition cages (14cm length, 14cm width and 22cm height), containing a fruit to stimulate oviposition (fixed in support) and two sponges under pet bottle caps to offer the diet (water and honey at 10%). The top of the cage was closed with voil fabric to promote aeration. Conclusion : The methodology used is suitable for mass rearing of avocado seed moth S. catenifer .Purpose : The increase in water intake has been studied as a method to decrease urine specific gravity in order to prevent urolithiasis, interstitial cystitis and feline urethral obstruction. This stud aims to understand if the type of drinking fountain will reflect in the increase of water intake and how this will influence the urine specific gravity and possible prevention of the diseases mentioned above. Methods: This study proposes to compare the amount of water ingested and which type of waterfall is preferred by felines through camera recording. Thirty healthy cats with a body weight of 3 to 6 kg (score 5 to 6) and range from 2 to 5 years. Results: To date, 22 animals have been evaluated, where partial results have shown that the feline normally has a preference for its usual source and new sources interfere in a negative way in its ingestion, in addition, it was observed that places where there is more than one animal, dominant feline is the one that ingests greater amount of water and prevents other felines from ingesting it, generating fear in the others. Conclusions: Thus, the implementation of new sources tends to be a disadvantage among felines and the dominance factor between them also interferes in these results.Purpose: The parasitoid Cotesia flavipes Cameron (Hymenoptera: Braconidae), used in the control of the sugarcane borer Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) is applied in about 3.5 million hectares in Brazil. Currently, 4 to 8 plastic containers are released manually, corresponding to about 6 thousand parasitoids per hectare. In this way, workers walk between the lines of culture to make the release. The objective of this work was to verify the longevity of adults in plastic and biodegradable packaging, trying to provide a packaging that is environmentally adequate, has rapid degradation in the field and allows the release of parasitoids using drone. Methods: As soon as the insects emerged, two pairs were individualized per pack, observing 10 repetitions for each package. The evaluations were carried out every 4 hours until the death of the insects that were kept under controlled conditions (temperature of 25 ± 1 °C and photoperiod of 12h of light/12h of dark) and remained without food. Results: The longevity of adults was influenced by the packages. The males showed longevity of 36.4 hours in the plastic packages and 25.5 hours in the biodegradable ones. The females showed longevity of 37.4 hours in the plastic packages and 27.0 hours in the biodegradable ones. Conclusion : The plastic packages provided greater longevity of the adults of the C. flavipes parasitoid. However, parasitoids can be released in the field using the biodegradable packages in drones if the release occurs soon after the emergence of adults.Objective: Once the three rhetorical fundamental pillars are stablished as ethos , pathos , and logos , the aim of this paper was to unveil the Aristotelian passions, based on the pathos sphere, in a musical-rhetorical corpus – i.e. the image-sound bond in a cinematographic piece. The corpus is composed by the sixth episode of the 2014 Argentinean movie, Wild Tales . Entitled Till Death do us Apart , the episode deflagrates the perfect bond between image and sound and how this relationship is powerful concerning the persuasion matter. Methods: In order to achieve this conclusion, we performed a rhetorical analysis based on both: Musical – Arakawa (1995), Bennett (1990), and Copland (1974) – and Rhetorical – Aristotle (2000), Meyer (2007), Reboul (2004), Ferreira (2010), Abreu (2001), and Fiorin (2014) – theories. The objective was to consider how the image-sound bond could evoke and project the Aristotelian passions from the plot to the audience, turning this process into persuasion. Results: The preliminary results showed us that the image-sound bond, in a cinematographic sphere, can be used as a bridge to awaken emotions, intended by the plot, in the audience, turning the movie meanings more tangible. Moreover, this bond can also create new nuances, bringing new meanings to the piece. Through our rhetorical analysis it was possible to deflagrate the power of Music concerning Rhetoric and persuasion in a cinematographic piece.

CHEMICAL PROFILE AND SCHISTOSOMICIDAL INVESTIGATION OF A MARINE-DERIVED FUNGUS Penicillium citrinum

Purpose: The objective of this work will be to identify the nutritional and alimentary impact during the treatment of patients with head and neck neoplasm. Methods: The present study will be carried out in patients of the Hospital do Cancer de Franca-SP, with the authorization of the coordinator responsible for the hospital and later the Research Ethics Committee - CEPE of Santa Casa de Franca. The research will be developed through a retrospective analysis of medical records of patients presenting with head and neck neoplasm, male and female, over 18 years of age, from June 2017 to May 2018 at Hospital do Cancer de Franca. Firstly, medical records of all patients with head and neck cancer who are undergoing antineoplastic treatment will be evaluated, covering the methods of radiotherapy, chemotherapy and surgeries. The analysis will be performed to collect information on anthropometric, biochemical, clinical and dietary data of certain patients. The variables to be analyzed will receive descriptive statistical treatment through tables and frequency graphs and for variables of a quantitative nature, the calculation of descriptive parameters such as arithmetic mean, median, standard deviation and coefficient of variation will complete the descriptive process. Results: The worlds cancer incidence rate exceeds 14 million people per year, and by 2030 it is estimated that more than 21 million new cases in the world. The present work is in the final phase of data collection and the next step will be the treatment and discussion of the data.Purpose: To design, construct and validate a system with efficiency of electric and thermal energy generation through refrigerated photovoltaic panels (hybrids). The focus of this work will be to optimize the energy efficiency of the photovoltaic panel and consequently to obtain the thermal energy generation with the energy dissipated by the system. Methods: Through a literature review from renowned authors, books and scientific articles, as well as a research applied through the design, construction and validation of a prototype, the experimental data of the panels are being collected through appropriate instruments, as well as validation of the modeling through data analysis and comparison between the techniques of capturing solar energy and cooling of the panels that best corresponds to the efficiency of the project. Results: The photovoltaic panel loses efficiency when its temperature increases. Although we have advanced well in the project, until the writing of this summary we were still testing the refrigeration system that provides better efficiency. Conclusion: From the study and experiences that have been and will still be, it was expected that the refrigerated panel would provide a greater generation of electrical energy in relation to the non-refrigerated and consequently already heat the water, thus obtaining a better use of energy.Purpose: The Community Group of Mental Health is characterized by an intervention modality with the purpose of promoting mental health, the dynamics of this group construction develops in a welcoming environment through the interaction between person and daily life thus promoting personal and collective growth. Method: Therefore, in the context of this research, it is emphasized the relevance of the use of this modality of group intervention for the promotion of mental health in the psychologist’s academic development, taking into account the statistic’s increase of psychological vulnerability in the university environment. The researchs main goal is to collect and analyze productions in the area of community groups on mental health and the psychologists training in the period from 2012 to 2017, The researchs main goal is to collect and analyze productions in the area of community groups on mental health and the psychologists training in the period from 2012 to 2017, aiming to characterize the focus of these productions and their contributions in psychology training. Results: The methodology used was a descriptive-qualitative research, through the integrative review of the scientific productions in the area during the period from 2012 to 2017 in PUBmed, BVsalud, Scielo and CAPES electronic scientific journals, using as descriptors mental health in university students and community mental health groups. The analysis was created integrating the results of the productions on Community Mental Health Groups with surveys about the psychological suffering in the graduations context. Among the works found three are in English and five in Spanish. Conclusion: The scientific productions in the search made addressed themes related to psychological vulnerability in the university environment as well as on Community Groups on Mental Health.Objective: This research deals with the new extra-judicial usucapion procedure, introduced in the legal system by the New Civil Procedure Code (Law 13.015/15). In his art. 1,071, the New Civil Code added art. 216-A in the Law n.6.015/73 (Public Registers), and established, in its paragraphs, a list of legal requirements for acquisition of property (real state) by extrajudicial misappropriation. Methods: The research aims at the interpretation of the norms that make up the legal institute of extrajudicial usucapion; analyzes interrelated norms of equal or different hierarchies in the legal system, starting with the Federal Constitution; analyzes the infraconstitutional legislation and normative acts that regulate the extra-judicial usucapion; analyzes technical reports from notary public registries and court decisions, in order to understand the operational aspects of the legal institute researched. Results: The results are partial, with positive and negative aspects and some controversial points, since its application is still in the phase of adaptation by the notaries. There have been practical doubts, however, in view of this, on 14/12/2017, the National Council of Justice – CNJ – brought to public attention a document, which established several guidelines for the procedure of extrajudicial misappropriation in notary public registries and public real estate registries. Conclusion: The extrajudicial misappropriation procedure allowed the recognition by extrajudicial way of all the usucapion modalities foreseen in the Brazilian law, provided that all the legal requirements are met. Thus, the present study aimed to study the new procedure, which brought speed and quick resolution of the demands, but did not exclude the discretion of the judiciary, since the procedure is optional, so it will be up to the interested party to choose. Keywords: unfairness; procedure; usucapion extrajudicial. Acknowledgments: CAPES, CNPqPurpose: the aim of this study was to evaluate the in vitro efficacy of three differents decontaminating treatments on oral bacterial biofilms developed on the surface of pacifiers made with silicone or latex. Methods: on the surfaces of 96 pacifiers made of silicone and latex of two comercial brands, biofilms were developed from the following reference strains (ATCC): Streptococcus mutans (25175) and Lactobacillus casei (11578). The pacifiers were then divided into 4 groups (N=12) according to the treatments: control group (without treatment), boiling water (1 minute), microwave (7 minutes), and chlorhexidine digluconate solution at 0.12 % (spray 6 times, 0.6 mL). The data were submitted to one-way ANOVA for repeated measures. The significance level established was of 5%. Results: at the moment, the data are under statistical treatment and will be available for presentation at the event. Conclusions: the conclusions will be formulated from the results of the statistical analysis.Purpose: Total Quality Management (TQM) is based on the application of 7 principles of quality to cover all aspects of an organization and ensure the total quality of the product offered to the customer. This article had as objective to analyze the implementation of the seven principles of TQM in civil construction. Methods: A descriptive and explanatory case study was conducted. We interviewed the Quality Manager with the objective of understanding the techniques and actions of quality management in projects of a large construction company located in the interior of the State of Sao Paulo. Results: It was possible to highlight the importance of the involvement of each collaborator of the organization, the need for top management support and the influence that TQM exerts on decision making and the product delivered to the final customer. Conclusion: We conclude that the scope of quality management is associated to several aspects of an organization, and a good management and the degree of satisfactory involvement of its employees is necessary. The use of TQM provides total quality assurance and a positive outlook for continuous improvement, which benefits the maintenance of the companys competitiveness in the market.Purpose: Although the standardization of disinfection processes by national and international organizations, there is a contamination of dialysis machines caused by fungus, especially in the biofilm growth (microorganisms held together by exopolymeric substance and adhered to a surface), has increased patients risk of acquiring infection. In addition, concentration of nutrients in the ambient, salinity, pH and oxygenation modulate the biofilm formation. The anti-biofilm activity of peracetic acid, a disinfectant recently introduced for the disinfection of hemodialysis machines, against Fusarium biofilms was evaluated under two oxygenation conditions. The concentrations tested were 0.1% and 0.2%, with 30 minutes and 10 minutes exposure duration, respectively. Methods: The biofilms were formed in microaerophilic and aerophilic conditions in 96-well microplates at 30 ° C for 72 hours, determining their cell viability by the tetrazolium salt reduction assay (XTT). Results: The results showed that 0.2% peracetic acid during 10 minutes effectively reduced the metabolic activity of Fusarium biofilms (p<0.05). Comparative analysis between aerophilia and microaerophilia showed higher efficiency for the microaerophilic condition (p <0.001). Conclusion: The 0.2% peracetic acid with a 10 min-exposure time showed to be a potent agent for disinfection of the dialysis machines infected with Fusarium biofilms. Disinfection efficiency can contribute to a better quality of life for patients who require renal replacement therapy, as well as reduce the costs of the public health system, reducing hospitalization for infection.Introduction: Neglected tropical diseases, a diverse group of diseases that prevail in tropical and subtropical conditions in 149 countries. Schistosomiasis is the third most devastating tropical disease in the world, being a major source of morbidity and mortality for developing countries. The treatment and control of schistosomiasis virtually relies on a single drug, praziquantel. The pressing need to develop new antischistosomal compounds has been emphasized, particularly in view of blanket application of praziquantel within the frame of preventive chemotherapy, a strategy that might select for drug-resistant parasites. Purpose: Here, we report the in vitro effect of crude extract and fractions of Baccharis dracunculifolia green propolis on Schistosoma mansoni adult worms . Methods: Green propolis was subjected to exhaustive maceration with ethanol. The obtained ethanol solution was filtered, concentrated and then submitted to liquid/liquid partition using different solvents. The obtained fractions, as well as the crude ethanolic extract, were analyzed by HPLC/DAD and evaluated in schistosomicidal assays Results: The crude ethanolic extract of Baccharis dracunculifolia green propolis (at a concentration of 200 μg/mL) showed substancial results, killing 93.75% of the Schistosoma mansoni worms. Dichloromethane and hexane fractions showed the greatest schistosomicidal effect (93.75% and 87.5%, respectively) while ethyl acetate and butanol fractions did not show schistosomicidal activity at the same concentration evaluated. Conclusion: Based on chemical and biological evaluation, the two main fractions of green propolis were selected for further study of isolation and identification of compounds with schistosomicidal activity.Purpose: Stenoma catenifer (Lepidoptera: Elachistidae), avocado seed moth, is considered a key pest in avocado because of the direct damages caused to the fruits by the entrance of the larvae and the construction of galleries. Thus, the objective was to determine a methodology of laboratory rearing to obtain insects for research on it behavior, biology, ecology and mainly control. Methods: The beginning of the laboratory establishment was with insects collected in organic crop of avocado located in Franca, SP (20 ° 32’19’’S 47 ° 24’03’’W). The insects were taken to the Laboratory of Agricultural Entomology of Unifran and the material was screened to obtain the S. catenifer larvae. Results: The fruits containing larvae were packed in transparent plastic containers until the pupae were formed (36cm length, 25.5cm width and 12.5 height). The pupae were transferred to other transparent plastic containers (11cm length, 11cm width and 7.5cm height) containing a layer of vermiculite to fix the pupae. In the adult phase, couples were placed in mate and oviposition cages (14cm length, 14cm width and 22cm height), containing a fruit to stimulate oviposition (fixed in support) and two sponges under pet bottle caps to offer the diet (water and honey at 10%). The top of the cage was closed with voil fabric to promote aeration. Conclusion : The methodology used is suitable for mass rearing of avocado seed moth S. catenifer .Purpose : The increase in water intake has been studied as a method to decrease urine specific gravity in order to prevent urolithiasis, interstitial cystitis and feline urethral obstruction. This stud aims to understand if the type of drinking fountain will reflect in the increase of water intake and how this will influence the urine specific gravity and possible prevention of the diseases mentioned above. Methods: This study proposes to compare the amount of water ingested and which type of waterfall is preferred by felines through camera recording. Thirty healthy cats with a body weight of 3 to 6 kg (score 5 to 6) and range from 2 to 5 years. Results: To date, 22 animals have been evaluated, where partial results have shown that the feline normally has a preference for its usual source and new sources interfere in a negative way in its ingestion, in addition, it was observed that places where there is more than one animal, dominant feline is the one that ingests greater amount of water and prevents other felines from ingesting it, generating fear in the others. Conclusions: Thus, the implementation of new sources tends to be a disadvantage among felines and the dominance factor between them also interferes in these results.Purpose: The parasitoid Cotesia flavipes Cameron (Hymenoptera: Braconidae), used in the control of the sugarcane borer Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) is applied in about 3.5 million hectares in Brazil. Currently, 4 to 8 plastic containers are released manually, corresponding to about 6 thousand parasitoids per hectare. In this way, workers walk between the lines of culture to make the release. The objective of this work was to verify the longevity of adults in plastic and biodegradable packaging, trying to provide a packaging that is environmentally adequate, has rapid degradation in the field and allows the release of parasitoids using drone. Methods: As soon as the insects emerged, two pairs were individualized per pack, observing 10 repetitions for each package. The evaluations were carried out every 4 hours until the death of the insects that were kept under controlled conditions (temperature of 25 ± 1 °C and photoperiod of 12h of light/12h of dark) and remained without food. Results: The longevity of adults was influenced by the packages. The males showed longevity of 36.4 hours in the plastic packages and 25.5 hours in the biodegradable ones. The females showed longevity of 37.4 hours in the plastic packages and 27.0 hours in the biodegradable ones. Conclusion : The plastic packages provided greater longevity of the adults of the C. flavipes parasitoid. However, parasitoids can be released in the field using the biodegradable packages in drones if the release occurs soon after the emergence of adults.Objective: Once the three rhetorical fundamental pillars are stablished as ethos , pathos , and logos , the aim of this paper was to unveil the Aristotelian passions, based on the pathos sphere, in a musical-rhetorical corpus – i.e. the image-sound bond in a cinematographic piece. The corpus is composed by the sixth episode of the 2014 Argentinean movie, Wild Tales . Entitled Till Death do us Apart , the episode deflagrates the perfect bond between image and sound and how this relationship is powerful concerning the persuasion matter. Methods: In order to achieve this conclusion, we performed a rhetorical analysis based on both: Musical – Arakawa (1995), Bennett (1990), and Copland (1974) – and Rhetorical – Aristotle (2000), Meyer (2007), Reboul (2004), Ferreira (2010), Abreu (2001), and Fiorin (2014) – theories. The objective was to consider how the image-sound bond could evoke and project the Aristotelian passions from the plot to the audience, turning this process into persuasion. Results: The preliminary results showed us that the image-sound bond, in a cinematographic sphere, can be used as a bridge to awaken emotions, intended by the plot, in the audience, turning the movie meanings more tangible. Moreover, this bond can also create new nuances, bringing new meanings to the piece. Through our rhetorical analysis it was possible to deflagrate the power of Music concerning Rhetoric and persuasion in a cinematographic piece.

Genotoxic and Chemopreventive Effects of Vochysia divergens Leaves (Pantanal, Brazil)

The medicinal plant Vochysia divergens is a colonizing tree species of the Pantanal, a unique and little explored wetland region in Brazil. This species is used in folk medicine as syrups and teas to treat respiratory infections, digestive disorders, asthma, scarring, and skin diseases. The objectives of this study were to evaluate the antioxidant, cytotoxic, and genotoxic potential of the ethanolic extract of Vochysia divergens leaves (VdE), as well as the influence of VdE and its major component (the flavone 3′,5-dimethoxy luteolin-7-O-β-glucopyranoside; 3′5 DL) on MMS-induced genotoxicity. The extract significantly reduced the viability of V79 cells in the colorimetric XTT assay at concentrations ≥ 39 μg/mL. A significant increase in micronucleus frequencies was observed in V79 cell cultures treated with VdE concentrations of 160 and 320 μg/mL. However, animals treated with the tested doses of VdE (500, 1000, and 2000 mg/kg b.w.) exhibited frequencies that did not differ significantly from those of the negative control group, indicating the absence of genotoxicity. The results also showed that VdE was effective in reducing MMS-induced genotoxicity at concentrations of 20, 40, and 80 μg/mL in the in vitro test system and at a dose of 15 mg/kg b.w. in the in vivo test system. Its major component 3′5 DL exerted no protective effect, suggesting that it is not responsible for the effect of the extract. The results of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay showed that VdE was able to scavenge 92.6% of free radicals. In conclusion, the results suggest that the protective effect of VdE may be related, at least in part, to the antioxidant activity of its chemical constituents.

Development and validation of an HPLC-DAD analytical method to quantify 5-methoxyflavones in methanolic extracts of Vochysia divergens Pohl cultured under stress conditions

Letícia Pereira Pimentaa, Luís Claúdio Kellner Filhoa, Bruna Arruda Soares Parpinellib, Katia Aparecida de Siqueirab, Marcos Antônio Soaresb, Márcio Luís Andrade e Silvaa, Wilson Roberto Cunhaa, Patrícia Mendonça Paulettia and Ana Helena Januárioa,* Núcleo de Pesquisas em Ciências Exatas e Tecnológicas, Universidade de Franca, 14404-600 Franca – SP, Brasil Laboratório de Biotecnologia e Ecologia Microbiana, Departamento de Botânica e Ecologia, Universidade Federal de Mato Grosso, 78060-900 Cuiabá – MT, Brasil