H. Y. Tang

The Efficacy of MS-222 as Anesthetic Agent in Largemouth Bronze Gudgeon Coreius guichenoti

AbstractThe Largemouth Bronze Gudgeon Coreius guichenoti is a vulnerable and valuable fish in the upper Yangtze River of China. It is essential to keep it calm by using anesthetic in routine operations such as weight measurement, tag injection, and artificial spawning. This study was conducted to investigate the efficacy of different concentrations of MS-222 (tricaine methanesulfonate) as an anesthetic agent in C. guichenoti. The results showed that anesthesia time decreased significantly (P < 0.001) with increasing anesthetic concentrations. However, anesthetic concentrations had no significant effect on recovery time. In addition, anesthesia time had an obvious positive correlation with body weight. Overall, MS-222 was an effective anesthetic agent for C. guichenoti. Moreover, the optimal concentrations of MS-222 in C. guichenoti (average body weight =135.29 ± 2.23 g) were 100, 120, 160, and 180 mg/L.Received June 27, 2016; accepted September 30, 2016 Published online December 14, 2016

The complete mitochondrial genome sequence of Triplophysa anterodorsalis (Teleostei, Balitoridae, Nemacheilinae)

Abstract Triplophysa anterodorsalis is an endemic fish in the upper stream of the Yangtze River, Jinsha River and its tributaries. However, wild populations of T. anterodorsalis declined sharply due to cascade hydropower stations constructed successively in the Jinsha River during the past decades. In the present study, the complete mitochondrial DNA genome sequence of T. anterodorsalis was first determined by DNA sequencing based on the PCR fragments. The complete mitochondrial genome sequence of T. anterodorsalis is a circular molecule of 16,567 bp in size. It consists of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and a control region (D-loop). The gene nucleotide composition of T. anterodorsalis is 27.37% A, 25.68% C, 18.37% G, and 28.57% T, with a relatively a relatively high A + T content (55.94%). The results could provide useful data for studies on genetic structure and diversity and rational resource conservation in T. anterodorsalis.

Isolation and characterization of microsatellite loci in Jinshaia sinensis

In this study, we isolated and characterized thirteen polymorphic microsatellite markers for Jinshaia sinensis, a fish species endemic to the upper reaches of the Yangtze River in China. Each locus was screened in a population of 48 individuals. Number of alleles per locus ranged between five and nineteen. Observed heterozygosity ranged between 0.121 and 0.854, and expected heterozygosity between 0.722 and 0.928. No significant linkage disequilibrium was found between pairs of loci. However, three loci showed significant departures from Hardy-Weinberg equilibrium and four loci had evidence of null alleles. These markers presented here will be valuable tools to understand the genetic structure of J. sinensis populations in the Yangtze River.

The complete mitochondrial genome of Schizothorax chongi (Fang, 1936) (Teleostei, Cyprinidae, Schizothoracinae).

Abstract Schizothorax chongi is an endemic and important polyploidy fish in the upper stream of the Yangtze River. S. chongi represents a typical model species to study historical adaptation and evolution in the Tibetan Plateau. In this study, the complete mitochondrial DNA genome sequence of S. chongi was first determined by DNA sequencing based on the PCR fragments. The complete mitochondrial DNA (mtDNA) genome sequence of S. chongi is a circular molecule of 16,584 bp in length. It consists of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and a control region (D-loop). The gene nucleotide composition of S. chongi is 29.6% A, 27.1% C, 17.9% G, and 25.4% T, with a high AT content (55.0%). The results could provide useful data for further studies on phylogenetics, conservation genetics and rational resource management for S. chongi.

Characterization of the complete mitochondrial genome sequence of Homatula potanini (Cypriniformes, Nemacheilidae, Nemachilinae)

Abstract Homatula potanini is an endemic and one of ornamental fishes in the upper stream of the Yangtze River and its tributaries. However, wild populations of H. potanini declined sharply due to anthropological activity in the Jinsha River during the past decades. In present study, the complete mitochondrial DNA genome sequence of H. potanini was first determined by DNA sequencing based on the PCR fragments. The complete mitochondrial genome sequence is a circular molecule of 16,569 bp in size. It consists of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and a control region (D-loop). The gene nucleotide composition of H. potanini is 30.1% A, 26.9% C, 16.7% G, and 26.3% T, with a relatively high A + T content (56.4%). The results could provide useful data for studies on genetic structure and rational resource conservation in H. potanini.

The complete mitochondrial genome of Glyptothorax sinense (Siluriformes, Sisoridae).

Abstract Glyptothorax sinense (Siluriformes, Sisoridae), is a kind of small-sized freshwater fish which mainly distributes in the middle and upper reaches of the Yangtze River in China. In this study, the complete mitochondrial genome of G. sinense was first determined using a PCR-based method. The complete mtDNA sequence is 16,531 bp in length, including 22 transfer RNA genes, 2 ribosomal RNA genes, 13 protein-coding genes, and a non-coding control region (D-loop). The overall-based composition was 31.61% A, 26.66% T, 15.38% G and 26.34% C, with a relatively high A + T content (58.27%). This will provide a useful tool for evolutionary and population genetic studies of G. sinense.

Isolation and characterization of 11 microsatellite loci in Schizothorax wangchiachii (Fang)

Abstract72 microsatellite loci were isolated from a (GATA)8 enriched genomic library of Schizothorax wangchiachii (Fang), an endemic fish species in the upper reaches of the Yangtze River. A population of 32 wild captured individuals of this species was tested by these 72 primer pairs developed, 11 pairs of which exhibited polymorphic. The number of observed alleles per locus ranged from 2 to 7. Observed and expected heterozygosities ranged from 0.344 to 0.875 and from 0.289 to 0.875, respectively. No significant linkage disequilibrium was detected among the loci. One loci showed significant deviation from Hardy–Weinberg equilibrium and none was detected in linkage equilibrium. These microsatellite loci described for S. wangchiachii will be useful for future study on population structure, breeding, and conservation studies.