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Featured researches published by H Yan.


Transplantation Proceedings | 2000

Evidence of regulatory T lymphocytes that constitute peripheral blood microchimerism following rat liver transplantation.

K. Adachi; Akihiko Tamura; Atsushi Sugioka; Miwa Morita; H Yan; X.-K Li; Yusuke Kitazawa; Hiroshi Amemiya; Seiichi Suzuki; Miyata M; Hiromitsu Kimura

IT has been well established that, unlike other vascularized allografted organs, hepatic transplants in various species such as pigs or rats of some strain combinations are spontaneously accepted across a whole MHC class I plus class II barrier without active immunotherapy. In addition, spontaneous acceptance of a liver allograft is accompanied by systemic immunological tolerance of other grafts from donor strains, but not of a third party. Furthermore, it has been shown that the tolerance induced by liver allografting is able to reverse ongoing rejection of heart. The mechanisms underlying this unique phenomenon might be of potential use in a clinical setting. In this respect, spontaneous liver allograft acceptance has been suggested to be due to some characteristics of the liver per se, such as its ability to make large amounts of soluble class I antigen, or its complex with antibodies, which may interfere with rejection. Alternatively, it has been suggested that passenger leukocytes within the transplanted liver result in host microchimerism and thereby induce tolerance. If liver passenger leukocytes induce tolerance, then they may bear different characters than those from other organs, since the original concept of passenger leukocytes indicated that the immunogenicity of transplanted organs was attributed to these cells. Thus, it has been shown that several maneuvers, such as removal of passenger leukocytes from these grafts by irradiation and/or pretreatment of the donor by radiomimetic chemical or by parking the graft in an intermediate host syngeneic with the recipient, prolongs allograft survival. In this study we examined whether and to what extent a population of liver passenger leukocytes are important in liver allograft acceptance and its relationship to microchimerism in the peripheral blood by treating them by donor irradiation.


Transplantation | 2004

STUDIES ON THE MOST EFFICIENT VECTOR SYSTEMS FOR GENE TRANSDUCTION INTO DENDRITIC CELLS (DC)

E. Satoh; H Yan; Tohko Miyagi; Xiao-Kang Li; Wataru Sugiura; Naoki Yamamoto; Kenichi Teramoto; Shigeki Arii; Hiromitsu Kimura

Specialized antigen-presenting cells (APC), known as dendritic cells (DC), play a pivotal role in initiating primary immune responses. Several vector systems, including adenoviral vectors, retroviral vectors, hemagglutinating virus of Japan-related vectors, and the electroporation, have been shown to transduce genes into mouse and human but not rat DC. However, there is no direct evidence to support the view that the currently used vector systems are able to transduce genes into mature DC. Inasmuch as most, if not all, gene transfer studies investigating DC or DC-related cell populations are performed employing heterogeneous-groups of cells, it is therefore important to determine the extent to which gene transduction occurs in bona fide DC. In this study, we provide evidence that none of these vector systems are able to transfer genes into mature rat DC, which are derived from bone marrow cells (BMC), driven by Flt3/Flk2 ligand and IL-6, and purified with CD161a. Nevertheless, the most efficient gene transduction was observed with developing DC progenitor cells during long-term culture of rat BMC. Successful gene transfer was achieved after 2-week culture with an HIV-based lentiviral vector system.


Transplantation | 2004

PHENOTYPE AND FUNCTIONAL IDENTITY OF GM-CSF INDEPENDENT DENDRITIC CELLS GENERATED BY LONG-TERM PROPAGATION OF DC PROGENITOR CELLS IN BONE MARROW CELLS AND SKIN LANGERHANS CELLS

H Katayama; Y Hattori; K Ogata; H Yan; E. Satoh; Kenichi Teramoto; Shigeki Arii; R Kamide; H Kimua

Evidence is provided that dendritic cells (DC) generated by either long-term bone marrow cell (BMC) culture with Flt3L and interleukin-6 (IL-6), or after short-term BMC culture with granulocyte macrophage-colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4), contain heterogeneous cell populations of admixed DC and Mphi, regardless of the cytokine source. By employing GM-CSF-independent culture systems with the aid of Flt3/Flk-2 ligand and IL-6 and phenotypic characterization of BMC-derived DC and skin Langerhans cells (LC), revealed similar phenotypes. Furthermore, CD103 (OX62), which is widely used for rat DC separation, was found to be insufficient to enrich DC, due to downregulation of the marker. In this regard, the most efficient selection of rat DC, was obtained by CD161a (NKR-P1A), a member of the C-type lectin family. Despite the phenotypic similarity with BMC-derived DC, the nucleus of LC showed a distinct morphology. A large population of DC generated by Flt3L/IL-6 from GM-CSF receptor-deficient mice by do not express NK1.1 (NKR-P1B and NKR-P1C). The profiles for BMC-derived DC were the same as for skin Langerhans cells.


Molecular Therapy | 2004

406. Determination of the Most Efficient Vector System for Gene Transduction into Dendritic Cells (DC)

E. Satoh; H Yan; Xiao-Kang Li; Wataru Sugiura; Naoki Yamamoto; Kenichi Teramoto; Shigeki Arii; Hiromitsu Kimura

Background: It has been well established that the specialized antigen presenting cells (APC), dendritic cells (DC), play a pivotal role in initiating a primary immune response. Although it has been reported that several vector systems including Adenoviral vector, HVJ liposome, inactivated HVJ vector, and electroporation-based plasmid vector systems, are able to transduce the genes into the DC in mouse and human, this is not the case with rat DC. In addition to this, it appears that the reported studies were not always employing highly purified DC and, to our best knowledge, there has been no direct evidence to support the view that currently used-vector systems are able to effectively transduce the gene into DC. Inasmuch as most, if not all, DC or DC-related gene-transfer studies are performed by employing heterogeneous cell populations, it is important to determine whether and to what extent each vector system is able to transduce the gene into DC.


Transplantation Proceedings | 1998

Prevention of Experimentally Induced Autoimmune Type I Diabetes in Rats by the New Immunosuppressive Reagent FTY720

Kazuya Suzuki; H Yan; X.-K Li; Hiroshi Amemiya; Seiichi Suzuki; K. Hiromitsu


Transplantation Proceedings | 1998

Immunosuppressive effect of FTY 720 on autoimmune diabetes models

H Yan; Kazuya Suzuki; X.-K Li; Hiroshi Amemiya; Seiichi Suzuki; K. Hiromitsu


Transplantation Proceedings | 2005

Phenotype and functional identity of GM-CSF–independent dendritic cells generated by long-term propagation of DC progenitor cells in bone marrow cells and skin langerhans cells

H. Katayama; Y. Hattori; K. Ogata; H Yan; E. Satoh; Kenichi Teramoto; Shigeki Arii; R. Kamide; H. Nakagawa; Hiromitsu Kimura


Transplantation Proceedings | 1998

Origin, occurrence, and function of microchimeric cells: V. quantitative aspects of microchimerism following pancreaticoduodenal transplantation model in rats

Kazuya Suzuki; Teruhisa Kazui; A Kawabe; H Yan; X.-K Li; Yusuke Kitazawa; Hiroshi Amemiya; Seiichi Suzuki; Hiromitsu Kimura


Transplantation Proceedings | 2005

Studies on the most efficient vector systems for gene transduction into dendritic cells

E. Satoh; H Yan; Tohko Miyagi; Xiao-Kang Li; Wataru Sugiura; Naoki Yamamoto; Kenichi Teramoto; Shigeki Arii; Hiromitsu Kimura


Transplantation | 2004

LIFE SPAN OF NAÏVE AND MEMORY T CELLS IN ADULT THYMETOMIZED RTAS

Y Hattori; Lei Guo; Y Nagata; H Yan; M Miyamoto; H Katayama; E. Satoh; Hiromitsu Kimura

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Hiromitsu Kimura

Tokyo Medical and Dental University

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E. Satoh

Tokyo Medical and Dental University

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Kenichi Teramoto

Tokyo Medical and Dental University

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Shigeki Arii

Tokyo Medical and Dental University

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Naoki Yamamoto

Tokyo Medical and Dental University

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