Hai-Long Wu

Rapid Determination of Bitertanol Content in Banana Using Three-dimensional Fluorescence Coupled with Second-order Calibration

Abstract A novel approach was developed for the direct quantitative analysis of bitertanol in banana by the incorporation of three-dimensional fluorescence with second-order calibration methodologies based on alternating trilinear decomposition (ATLD), alternating fitting residue (AFR), and self-weighted alternating trilinear decomposition (SWATLD) algorithms, respectively. The average recoveries of bitertanol in banana extract by using ATLD, AFR and SWATLD with an estimated component number of two were 98.2% ± 2.6%, 97.6% ± 2.1%, and 91.5% ± 3.5%, respectively. Moreover, the accuracy of these three algorithms was also evaluated through elliptical joint confidence region tests (EJCR) as well as figures of merit, such as sensitivity (SEN), selectivity (SEL), and limit of detection (LOD). The results demonstrate that these algorithms have been successfully applied to determine the content of bitertanol in banana extract.

Simultaneous Determination of Warfarin and Aspirin Contents in Biological Fluids Using Excitation-Emission Matrix Fluorescence Coupled with a Second-order Calibration Method

In the present work, a practical method that combines excitation-emission matrix fluorescence with a second-order calibration method based on an alternating trilinear decomposition (ATLD) algorithm was developed in order to simultaneously and directly determine the contents of warfarin (WAR) and aspirin (ASA) in human plasma and urine samples, even in the presence of unknown interferences. With the pre-estimated component number of 4, the obtained average spiked recoveries were 105.4 ± 7.8 and 104.2 ± 8.3% for WAR, 96.5 ± 2.8 and 91.2 ± 2.3% for ASA in human plasma and urine samples, respectively. Furthermore, the figures of merit were calculated and also inter- and intra-day experiments were performed that proved the proposed method is of great significance to the monitoring of clinical administration and also being a simple sample pretreatment at low-cost.