Hasan Serdar Gergerlioğlu

Effects of Coenzyme Q10 Supplementation on Plasma Adiponectin, Interleukin-6, and Tumor Necrosis Factor-α Levels in Men

The aim of the study was to determine the effects of coenzyme Q10 supplementation on plasma adiponectin, interleukin (IL)-6, and tumor necrosis factor (TNF )-alpha levels in sedentary men. Fourteen healthy, nonsmoking, sedentary men participated in the study. The protocol was approved by the Ethical Committee of our institution. This study was a randomized, double-blind, crossover trial. Blood samples were collected from all participants before coenzyme Q10 or placebo supplementation. The participants were randomly allocated to two groups. Seven participants received oral coenzyme Q10 (100 mg/day) supplementation, and seven participants received placebo (glucose) for 8 weeks. At the end of the 8 weeks, a second blood sampling was performed. After a 4-week washout period, placebo was given to the participants who used coenzyme Q10 the first time, and vice versa, and blood sampling was repeated. Plasma was stored at -80 degrees C until the time of analysis for adiponectin, IL-6, and TNF-alpha. Both CoQ10 and placebo supplementation did not affect plasma adiponectin and TNF-alpha levels. IL-6 level increased with coenzyme Q10 supplementation, but this increase did not differ from that seen with placebo supplementation. Coenzyme Q10 supplementation did not affect plasma adiponectin, IL-6, and TNF-alpha levels in sedentary men.

Antidepressant-like effects of quercetin in diabetic rats are independent of hypothalamic-pituitary-adrenal axis.

Objective Quercetin, one of the most potent flavonol in the family of flavonoids, has been shown to have benefits against diabetes and its complications. In the present study, we investigated effects of quercetin on depression-like behaviours and hypothalamic–pituitary–adrenal (HPA) axis in diabetic rats. Methods Experimental diabetes was induced by using streptozotocin, and either 50 or 100 mg/kg quercetin was intraperitoneally administered for 21 days. Following the last treatment, animals were subjected to the forced swim test, and subsequently, the blood was obtained by cardiac puncture to measure plasma adrenocorticotropic hormone (ACTH) and corticosterone (CORT) levels. Results A significant increase of the total immobile time, accompanied by a decrease in the immobility latency, which suggests a depressive status, was observed in diabetic animals that was reversed by the treatment of 50 mg/kg quercetin. However, the higher dose of quercetin (100 mg/kg) was ineffective in alleviating depression-like behaviours. The plasma concentrations of ACTH, and total- and free-CORT were not affected by both doses of quercetin. Conclusion Therefore, we concluded that the antidepressant-like effects of quercetin in diabetes are independent of the HPA axis.

Effects of repeated bouts of supramaximal exercise on plasma adiponectin, interleukin-6, and tumor necrosis factor-α levels in sedentary men.

Abstract Gökbel, H, Okudan, N, Gül, I, Belviranlı, M, Gergerlioğlu, HS, and Başaralı, MK. Effects of repeated bouts of supramaximal exercise on plasma adiponectin, interleukin-6, and tumor necrosis factor-&agr; levels in sedentary men. J Strength Cond Res 26(6): 1675–1679, 2012—The objective of the study was to determine the effects of repeated bouts of supramaximal exercise on plasma adiponectin, interleukin (IL)-6 and tumor necrosis factor (TNF)-&agr; levels in sedentary men. Fourteen healthy, nonsmoking, and sedentary men aged between 18.4 and 21.4 years participated in the study. All the subjects performed 5 Wingate tests (WTs) with 75 g per kilogram body weight load with 2-minute intervals between the tests. Blood samples were collected at preexercise, immediately after, 15 and 60 minutes after the fifth WT. Serum and plasma samples were stored at −80°C until the time of analysis for myoglobin, adiponectin, IL-6, and TNF-&agr;. Plasma adiponectin levels decreased, whereas IL-6 levels increased postexercise compared with that preexercise. The TNF-&agr; levels were not changed with supramaximal exercise. In conclusion, repeated bouts of supramaximal exercise cause an inflammatory response in exercised muscle and increase in plasma IL-6 levels and decrease in adiponectin concentrations.

Impact of enriched environment on production of tau, amyloid precursor protein and, amyloid-β peptide in high-fat and high-sucrose-fed rats.

Objective The Western-type diet is associated with an elevated risk of Alzheimer’s disease and other milder forms of cognitive impairment. The aim of the present study was to investigate the effects of the environmental enrichment on amyloid and tau pathology in high-fat and high-sucrose-fed rats. Methods In total, 40 adult male rats were categorised into two main groups according to their housing conditions: enriched environment (EE, n=16) and standard housing condition (n=24). The groups were further divided into five subgroups that received standard diet, high-fat diet, and high-sucrose diet. We performed the analysis of amyloid β-peptide (Aβ) (1–40), Aβ(1–42), amyloid precursor protein (APP), and tau levels in the hippocampus of rats that were maintained under standard housing conditions or exposed to an EE. Results The EE decreased the Aβ(1–40), Aβ(1–42), APP, and tau levels in high-fat and high-sucrose-fed rats. Conclusion This observation shows that EE may rescue diet-induced amyloid and tau pathology.