Henrik Böhlenius

CO/FT Regulatory Module Controls Timing of Flowering and Seasonal Growth Cessation in Trees

Forest trees display a perennial growth behavior characterized by a multiple-year delay in flowering and, in temperate regions, an annual cycling between growth and dormancy. We show here that the CO/FT regulatory module, which controls flowering time in response to variations in daylength in annual plants, controls flowering in aspen trees. Unexpectedly, however, it also controls the short-day–induced growth cessation and bud set occurring in the fall. This regulatory mechanism can explain the ecogenetic variation in a highly adaptive trait: the critical daylength for growth cessation displayed by aspen trees sampled across a latitudinal gradient spanning northern Europe.

GA4 Is the Active Gibberellin in the Regulation of LEAFY Transcription and Arabidopsis Floral Initiation

Flower initiation in Arabidopsis thaliana under noninductive short-day conditions is dependent on the biosynthesis of the plant hormone gibberellin (GA). This dependency can be explained, at least partly, by GA regulation of the flower meristem identity gene LEAFY (LFY) and the flowering time gene SUPPRESSOR OF CONSTANS1. Although it is well established that GA4 is the active GA in the regulation of Arabidopsis shoot elongation, the identity of the GA responsible for the regulation of Arabidopsis flowering has not been established. Through a combination of GA quantifications and sensitivity assays, we show that GA4 is the active GA in the regulation of LFY transcription and Arabidopsis flowering time under short-day conditions. The levels of GA4 and sucrose increase dramatically in the shoot apex shortly before floral initiation, and the regulation of genes involved in GA metabolism suggests that this increase is possibly due to transport of GAs and sucrose from outside sources to the shoot apex. Our results demonstrate that in the dicot Arabidopsis, in contrast with the monocot Lolium temulentum, GA4 is the active GA in the regulation of both shoot elongation and flower initiation.

Early development of pure and mixed tree species plantations in Snogeholm, southern Sweden

There is a lack of experimental studies comparing the forest production of mixed tree species stands and monocultures. As a case study, in 1994 an experiment was established in an afforestation landscape in southern Sweden with 66 plots: 18 planted with single tree species (including most native Swedish trees, plus Populus and Larix hybrids) and 48 with various non-replicated mixtures. Fifteen years after planting, stand growth did not differ significantly between the mixtures and the monocultures. However, the monocultures did exhibit a higher variation in growth levels relative to the moderate but comparatively consistent growth levels exhibited by the mixtures; particularly those comprised of more than two species. A specific analysis of mixtures containing either the tree species Picea abies or Quercus robur and their corresponding monocultures demonstrated the importance of the tree species admixed as a determinant of production outcomes early in the rotation. In the case of P. abies, tree species mixtures tended to reduce production, whereas in the case of Q. robur, mixtures tended to increase production. In addition no consistent differences in the mean height and mean diameter growth of P. abies and Q. robur between mixtures and monocultures were detected. A major conclusion is that adding more than two species did not increase stand volume growth.

Growth response of hybrid poplars to different types and levels of vegetation control

Poplar plantations established on agricultural or forest land can be adversely affected by abundant competing vegetation already present or that grows after planting. In this paper, we investigate how different intensities of manual vegetation control and mulching with polypropylene plastic effect poplar growth during the first two years after planting. Field data revealed that, during the first year, mulching with polypropylene plastic increased seedling growth more than manual vegetation control when performed over the same area. However, during the second year, these differences diminished and poplar seedlings continued to grow at the same rate irrespective of vegetation control. The difference in growth between treatments seems to be related to the fact that mulching positively influences water and nutrient availability to a greater extent that manual vegetation control during the early phase of establishment. Vegetation control also affected biomass allocation, with a relative increase in leaf biomass and decrease in stem biomass. The increase in leaf biomass associated with vegetation control was a result of both an increase in leaf number and increased leaf size.

Impact of seedling type on early growth of poplar plantations on forest and agricultural land

ABSTRACT Today, most poplar plantations in the temperate region are established on abandoned marginal agricultural land, but there is great potential for planting poplars on forest land as the available area is large and does not compete with food production. The objective of this study was to examine how different planting types (un-rooted cuttings, bare-rooted and containerized plants) affect the establishment and early growth of poplar plants on forest and agricultural sites. Our results suggest that on the agricultural site, survival and growth during the first two years are not influenced by plant type. However, at the forest sites, survival of rooted plants was superior compared to un-rooted cuttings. The height and biomass (stem and root) increment of bare-rooted plants was low; greater height and biomass growth was found for containerized plants. Container sizes had no effect on height growth, but leaf and stem biomasses were higher if the largest containers were used. When using the largest containers, concentrations of macronutrients (N, P) were increased compared to bare-rooted plants. Thus, these results suggest that practices for establishing poplar plantations of agricultural land include planting of un-rooted cutting, but on forest land, a plant grown in a container of 470 ml should be used. Together, this can reduce the cost of establishment, increase the available area for poplar plantations and have an impact on poplar plantation economics in Sweden.

Differences in Al sensitivity affect establishment of Populus genotypes on acidic forest land

Forest lands hold great potential for Populus plantations, but in native boreal forests, soils normally have low pH and thus higher levels of aluminum ions (Al3+ and hydroxides). Aluminum (Al) is one of the major factors limiting plant growth on these soils by inhibiting root growth, thus reducing water and nutrient uptake and slowing growth. There is a large variation in Al resistance both among and within species. In this study, growth responses of greenhouse-grown hybrid aspen (P. tremula × tremuloides) and poplar (P. trichocarpa hybrids) were monitored in relation to changes in Al concentrations. In quartz sand, hybrid aspen was more tolerant to exogenous application of Al than P. trichocarpa hybrids. This difference in Al-tolerance was further confirmed by hematoxylin staining of the roots, with hybrid aspen displaying less staining after Al treatment than poplar clones. When planted on forest land with low pH, hybrid aspen increased growth after planting and showed low mortality. This was not the case for poplar clones; plant height decreased after planting and mortality increased. Together, our results suggest that differences in initial growth and survival on forest land among hybrid aspen and the tested poplar clones may be connected to differences in Al tolerance. Our findings that staining with hematoxylin can identify Al-tolerant Populus genotypes may help identify Al-tolerant genotypes suitable for forest land.