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Dive into the research topics where Heung Joo Yuk is active.

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Featured researches published by Heung Joo Yuk.


Journal of Enzyme Inhibition and Medicinal Chemistry | 2017

Evaluation of polyphenols from Broussonetia papyrifera as coronavirus protease inhibitors

Ji-Young Park; Heung Joo Yuk; Hyung Won Ryu; Su Hwan Lim; Kyung Su Kim; Ki Hun Park; Young Bae Ryu; Woo Song Lee

Abstract The current study was designed to assess the inhibitory activity of Broussonetia papyrifera-derived polyphenols against 3-chymotrypsin-like and papain-like coronavirus cysteine proteases. The isolated compounds were broussochalcone B (1), broussochalcone A (2), 4-hydroxyisolonchocarpin (3), papyriflavonol A (4), 3′-(3-methylbut-2-enyl)-3′,4,7-trihydroxyflavane (5), kazinol A (6), kazinol B (7), broussoflavan A (8), kazinol F (9), and kazinol J (10). All polyphenols were more potent against papain-like protease (PLpro) than against 3-chymotripsin-like protease (3CLpro); therefore, we investigated their structural features that were responsible for this selectivity. Compound 4 was the most potent inhibitor of PLpro with an IC50 value of 3.7 μM. The active compounds displayed kinetic behaviors, and the binding constants of their interaction with PLpro were determined from surface plasmon resonance analysis. Our results suggest B. papyrifera constituents as promising candidates for development into potential anti-coronaviral agents.


Cytokine | 2017

3-Methoxy-catalposide inhibits inflammatory effects in lipopolysaccharide-stimulated RAW264.7 macrophages.

Hyung Won Ryu; Su Ui Lee; Seoghyun Lee; Hyuk-Hwan Song; Tae Hyun Son; Yeah-Un Kim; Heung Joo Yuk; Hyunju Ro; Chong-Kil Lee; Sung-Tae Hong; Sei-Ryang Oh

HIGHLIGHTS3‐Methoxy‐catalposide inhibits LPS‐increased expression of COX‐2 and iNOS.3‐Methoxy‐catalposide inhibits LPS‐increased expression of TNF‐&agr;, IL1&bgr; and IL‐6.3‐Methoxy‐catalposide inhibits both LPS‐activated MAP kinases and NF‐&kgr;B translocation into cell nucleus. ABSTRACT Pseudolysimachion rotundum var. subintegrum is utilized as a traditional herbal remedy to treat cough, bronchitis, and asthma in Korea, Russia, China, and Europe. Here, we show that 3‐methoxy‐catalposide, a novel iridoide glycoside isolated from P. rotundum var. subintegrum has the anti‐inflammatory activity in lipopolysaccharide (LPS)‐stimulated macrophages. The chemical structure of 3‐methoxy‐catalposide was determined by NMR, optical rotation and HRESIMS. In in vitro experiment, RAW264.7 cells were treated with 3‐methoxy‐catalposide for 2 h before exposure to LPS for different times. Inflammatory gene and protein expressions were assayed using RT‐PCR and ELISA. Activities of signal proteins were examined using western analysis. Our results demonstrated that 3‐methoxy‐catalposide significantly inhibits the expression of cyclooxygenase (COX)‐2 and inducible nitric oxide synthase (iNOS) in RAW264.7 cells stimulated by LPS, thereby suppressing the release of prostaglandin E2 (PGE2) and nitric oxide (NO). Moreover, 3‐methoxy‐catalposide markedly reduced the LPS‐induced expression of pro‐inflammatory genes, such as interleukin (IL)‐6, IL‐1&bgr;, and TNF‐&agr;. Further, 3‐methoxy‐catalposide inhibited both LPS‐induced activation of three MAP kinases (ERK 1/2, JNK, and p38) and the nuclear translocation of NF‐&kgr;B and AP‐1. These results support that 3‐methoxy‐catalposide may be a promising candidate for inflammation treatment.


Journal of Ethnopharmacology | 2018

Anti-inflammatory effect of stem bark of Paulownia tomentosa Steud. in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and LPS-induced murine model of acute lung injury

Jae-Won Lee; Kyeong-Hwa Seo; Hyung Won Ryu; Heung Joo Yuk; Hyun Ah Park; Yourim Lim; Kyung-Seop Ahn; Sei-Ryang Oh

ETHNOPHARMACOLOGICAL RELEVANCE The leaves, bark, and flowers of Paulownia tomentosa Steud. have been widely used as a traditional medicine in East Asia to treat inflammatory and infectious diseases. AIM OF THE STUDY We investigated the protective effect of the methanol stem bark extract of P. tomentosa using an animal model of lipopolysaccharide (LPS)-induced acute lung injury (ALI). MATERIALS AND METHODS The UPLC Q-TOF-MS profiles for the methanol extract of P. tomentosa stem bark showed that verbascoside and isoverbascoside were the predominant compounds. Raw 264.7 cells were used for inhibitory effects of cytokine production in vitro. C57BL/6N mice were administered intranasally with LPS (10μg/per mouse) to induce ALI. H&E staining was used to evaluate histological changes in the lung. RESULTS Treatment with P. tomentosa stem bark extract (PTBE) suppressed the production of IL-6 and TNF-α in LPS-stimulated RAW 264.7 macrophages, and the recruitment of neutrophils and macrophages in the BALF of mice with LPS-induced ALI. PTBE also decreased the levels of reactive oxygen species (ROS) and pro-inflammatory cytokines in the BALF. PTBE reduced the levels of nitric oxide (NO) in the serum and of inducible nitric oxide synthase (iNOS) in the lung of ALI mice. PTBE also attenuated the infiltration of inflammatory cells and the expression of monocyte chemoattractant protein-1 (MCP-1) in the lung. In addition, PTBE suppressed the activation of NF-κB and the reduced expression of superoxide dismutase 3 (SOD3) in the lung. CONCLUSION The results suggest that PTBE has a protective effect on LPS-induced ALI.


PLOS ONE | 2016

Anti-Obesity Effects of Spiramycin In Vitro and In Vivo.

Mun Ock Kim; Hyung Won Ryu; Ji-Hee Choi; Tae Hyun Son; Sei-Ryang Oh; Hyun-Sun Lee; Heung Joo Yuk; Sungchan Cho; Jong Soon Kang; Chang Woo Lee; Jinhyuk Lee; Chong-Kil Lee; Sung-Tae Hong; Su Ui Lee

The effects of spiramycin on adipogenesis and high fat diet (HFD)-induced obesity were investigated. Potential mechanisms contributing to these effects were elucidated. The inhibitory effect of spiramycin on adipocyte differentiation was assessed using 3T3-L1 preadipocyte cells, in which several parameters involved in AMPK signal pathways and lipid metabolism were examined. To further investigate the pharmacological effects of spiramycin in vivo, we examined several obesity-related parameters in HFD-induced obese mice. Spiramycin significantly inhibited preadipocyte differentiation by attenuating intracellular lipid accumulation. Spiramycin also reduced the expression of adipogenic master regulators (PPARγ, C/EBPα, and SREBP1c) and their downstream target genes (FAS, aP2, and GLUT4) in 3T3-L1 cells. In addition, AMPK phosphorylation was increased by spiramycin treatment in 3T3-L1 cells during early differentiation. Notably, HFD-induced obese mice administered spiramycin showed substantial decreases in body weight gain, serum leptin levels, adipose tissue mass, and hepatic lipid accumulation. Moreover, the decreased levels of GPT and GOT in the serum indicated that spiramycin attenuated hepatic injury caused by HFD. Taken together, these results demonstrate for the first time that spiramycin effectively attenuates HFD-induced obesity and hepatic steatosis by inhibiting adipogenesis.


Phytomedicine | 2018

Piscroside C inhibits TNF-α/NF-κB pathway by the suppression of PKCδ activity for TNF-RSC formation in human airway epithelial cells

Su Ui Lee; Seoghyun Lee; Hyunju Ro; Ji-Hee Choi; Hyung Won Ryu; Mun-Ock Kim; Heung Joo Yuk; Jinhyuk Lee; Sung-Tae Hong; Sei-Ryang Oh

BACKGROUND Piscroside C, isolated from Pseudolysimachion rotundum var. subintegrum, is a novel iridoid glycoside with therapeutic efficacy in a mouse model of chronic obstructive pulmonary disease (COPD). Piscroside C has been reported as a constituent of YPL-001 (under Phase 2a study, ClinicalTrials.gov identifier NCT02272634). PURPOSE To investigate the mechanisms behind piscroside C therapeutic effects on COPD in human airway epithelial NCI-H292 cells. METHODS We tested if piscroside C effectively suppresses MUC5AC gene expression and TNF-RSC/IKK/NF-κB cascades in TNF-α-stimulated NCI-H292 cells by employing, reverse transcription-polymerase chain reaction, enzyme-linked immunosorbent assay, luciferase reporter assays, chromatin immunoprecipitation assays and immunoprecipitation. RESULTS Piscroside C markedly suppressed the expression of TNF-α-induced MUC5AC mucus protein by inhibiting the transcriptional activity of NF-κB in NCI-H292 cells. Indeed, piscroside C negatively regulated the function of TNF receptor 1 signaling complex (TNF-RSC, an upstream regulator of the NF-κB pathway) without affecting its extracellular interaction with the TNF-α ligand. This inhibitory effect by piscroside C is mediated by the inactivation of protein kinase C (PKC), an essential regulator of TNF-RSC. PKC inactivation by piscroside C results in decreased PKCδ binding to a TRAF2 subunit of TNF-RSC and subsequent reduced IKK phosphorylation, resulting in NF-κB inactivation. CONCLUSION We propose that piscroside C is a promising therapeutic constituent of YPL-001 through its inhibition of PKCδ activity in the TNF-RSC/IKK/NF-κB/MUC5AC signaling cascade.


Bioscience, Biotechnology, and Biochemistry | 2017

Tripterygium regelii decreases the biosynthesis of triacylglycerol and cholesterol in HepG2 cells

Myung-Ji Kang; Eun-Bin Kwon; Heung Joo Yuk; Hyung Won Ryu; Soo-Yeon Kim; Mi-Kyeong Lee; Dong-Oh Moon; Su Ui Lee; Sei-Ryang Oh; Hyun-Sun Lee; Mun-Ock Kim

In the course of screening to find a plant material decreasing the activity of triacylglycerol and cholesterol, we identified Tripterygium regelii (TR). The methanol extract of TR leaves (TR-LM) was shown to reduce the intracellular lipid contents consisting of triacylglycerol (TG) and cholesterol in HepG2 cells. TR-LM also downregulated the mRNA and protein expression of the lipogenic genes such as SREBP-1 and its target enzymes. Consequently, TR-LM reduced the TG biosynthesis in HepG2 cells. In addition, TR-LM decreased SREBP2 and its target enzyme HMG-CoA reductase, which is involved in cholesterol synthesis. In this study, we evaluated that TR-LM attenuated cellular lipid contents through the suppression of de novo TG and cholesterol biosynthesis in HepG2 cells. All these taken together, TR-LM could be beneficial in regulating lipid metabolism and useful preventing the hyperlipidemia and its complications, in that liver is a crucial tissue for the secretion of serum lipids. The methanol extract of Tripterygium regelii leaves reduced intracellular contents of TG and cholesterol in HepG2 cells.


International Journal of Molecular Medicine | 2016

Picrasma quassiodes (D. Don) Benn. attenuates lipopolysaccharide (LPS)-induced acute lung injury.

Jae-Won Lee; Ji-Won Park; Na-Rae Shin; So-Yeon Park; Ok-Kyoung Kwon; Hyun Ah Park; Yourim Lim; Hyung Won Ryu; Heung Joo Yuk; Jung Hee Kim; Sei-Ryang Oh; Kyung-Seop Ahn


Journal of Functional Foods | 2016

The constituent, anti-inflammation, and human neutrophil elastase inhibitory activity of Gnaphalium affine

Hyung Won Ryu; Ki Ohk Kim; Heung Joo Yuk; Ok-Kyoung Kwon; Jung Hee Kim; Doo-Young Kim; Min Kyun Na; Kyung-Seop Ahn; Sei-Ryang Oh


Journal of Functional Foods | 2017

Protective effects of coumestrol on lipopolysaccharide-induced acute lung injury via the inhibition of proinflammatory mediators and NF-κB activation

Heung Joo Yuk; Jae-Won Lee; Hyun Ah Park; Ok-Kyoung Kwon; Kyeong-Hwa Seo; Kyung-Seop Ahn; Sei-Ryang Oh; Hyung Won Ryu


Journal of Functional Foods | 2017

Protective effect of polyacetylene from Dendropanax morbifera Leveille leaves on pulmonary inflammation induced by cigarette smoke and lipopolysaccharide

Jae-Won Lee; Hyung Won Ryu; Su Ui Lee; Tae Hyun Son; Hyun Ah Park; Mun Ock Kim; Heung Joo Yuk; Kyung-Seop Ahn; Sei-Ryang Oh

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Hyung Won Ryu

Korea Research Institute of Bioscience and Biotechnology

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Sei-Ryang Oh

Korea Research Institute of Bioscience and Biotechnology

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Kyung-Seop Ahn

Korea Research Institute of Bioscience and Biotechnology

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Hyun Ah Park

Korea Research Institute of Bioscience and Biotechnology

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Jae-Won Lee

Korea Research Institute of Bioscience and Biotechnology

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Su Ui Lee

Korea Research Institute of Bioscience and Biotechnology

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Ok-Kyoung Kwon

Korea Research Institute of Bioscience and Biotechnology

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Jung Hee Kim

Korea Research Institute of Bioscience and Biotechnology

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Sung-Tae Hong

Chungnam National University

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Tae Hyun Son

Korea Research Institute of Bioscience and Biotechnology

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