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Dive into the research topics where Hilde Sundvold is active.

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Featured researches published by Hilde Sundvold.


Biochimica et Biophysica Acta | 2008

Changes in fatty acids metabolism during differentiation of Atlantic salmon preadipocytes; effects of n-3 and n-9 fatty acids.

Marijana Todorčević; Anne Vegusdal; Tor Gjøen; Hilde Sundvold; Bente E. Torstensen; Marte Avranden Kjær; Bente Ruyter

Atlantic salmon (Salmo salar) preadipocytes, isolated from visceral adipose tissue, differentiate from an unspecialized fibroblast like cell type to mature adipocytes filled with lipid droplets in culture. The expression of the adipogenic gene markers peroxisome proliferated activated receptor (PPAR) alpha, lipoprotein lipase (LPL), microsomal triglyceride transfer protein (MTP), fatty acid transport protein (FATP) 1 and fatty acid binding protein (FABP) 3 increased during differentiation. In addition, we describe a novel alternatively spliced form of PPARgamma (PPARgamma short), the expression of which increased during differentiation. Eicosapentaenoic acid (20:5n-3, EPA) and docosahexaenoic acid (22:6n-3, DHA) lowered the triacylglycerol (TAG) accumulation in mature salmon adipocytes compared to oleic acid (18:1n-9, OA). This finding indicates that a reduced level of highly unsaturated n-3 fatty acids (HUFAs) in fish diets, when the traditional marine oil is exchanged for n-9 fatty acids (FAs) rich vegetable oils (VOs), may influence visceral fat deposition in salmonids. Moreover, major differences in the metabolism of EPA, DHA and OA at different stages during differentiation of adipocytes occur. Most of the EPA and DHA were oxidized in preadipocytes, while they were mainly stored in TAGs in mature adipocytes in contrast to OA which was primarily stored in TAGs at all stages of differentiation.


Lipids | 2003

An in vitro method for studying the proliferation and differentiation of Atlantic salmon preadipocytes

Anne Vegusdal; Hilde Sundvold; Tor Gjøen; Bente Ruyter

The aim of the present study was to develop a cell culture system for studying the proliferation and differentiation of preadipocytes isolated from Atlantic salmon adipose tissue. The expression of proliferating cell nuclear antigen (PCNA) was used as a marker for cell proliferation. The cells started to proliferate within 48 h after seeding and continued to proliferate throughout the culture period of 2 wk. Undifferentiated preadipocytes showed a fibroblast-like morphology with a homogeneous cytoplasm devoid of lipid droplets. At confluence, an exogenous lipid mixture was added to the cell cultures. The preadipocytes became larger and rounder during the subsequent days, and the cytoplasm gradually filled with lipid-rich droplets. These droplets were revealed by oil red O staining. Immunocytochemical staining showed that differentiated adipocytes expressed detectable levels of the three regulatory proteins associated with adipocyte differentiation: peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer binding protein α (C/EBPα), and leptin. The cells also showed activity of glycerol-3-phosphate dehydrogenase (GPDH) (EC 1.1.1.8), a biochemical marker of adipocyte differentiation. The morphological and biochemical data presented here show that fish preadipocytes have properties that are similar to those of preadipocytes in mammals. We conclude therefore that salmon adipose tissue contains a sizable population of preadipocytes. Exogenous lipids promote the activation of adipose-related genes and induce the differentiation of fish preadipocytes in vitro.


Comparative Biochemistry and Physiology B | 2008

Differential gene expression of fatty acid binding proteins during porcine adipogenesis.

Johanna Samulin; Ingunn Berget; Sigbjørn Lien; Hilde Sundvold

Four different subtypes of fatty acid binding proteins i.e. liver-type FABP1, heart/muscle-type FABP3, adipocyte-type FABP4 and epithelial/epidermal-type FABP5 are expressed in adipose tissue. However, only the regulatory role of FABP4 in adipogenesis has been thoroughly investigated. To increase the knowledge on possible roles of these FABP subtypes in preadipocyte differentiation, gene expression patterns were examined during adipogenesis in pig (Sus scrofa). FABP1 expression was induced in proliferating cells, whereas FABP3, FABP4 and FABP5 expression increased throughout preadipocyte differentiation. Interestingly, the FABP4 and FABP5 expression increased early in the differentiation, followed by FABP3 later in the differentiation process. This indicates a role of FABP4 and FABP5 in intracellular fatty acid transport during initiation of differentiation, whereas, FABP3 likely is involved in the transport of fatty acids during intermediate stages of adipogenesis. In this study we demonstrate that FABP3, FABP4 and FABP5 expression is correlated with that of the peroxisome proliferator-activated receptors alpha and gamma (PPARA and PPARG). Altogether, this suggests a role of FABP1 during cell proliferation, whereas a coordinated expression of FABP3, FABP4 and FABP5 together with that of PPARA, PPARG1 and PPARG2 might be critical for the metabolic regulation during porcine adipogenesis.


Gene | 2001

Tissue distribution of porcine peroxisome proliferator-activated receptor α: detection of an alternatively spliced mRNA

Hilde Sundvold; E. Grindflek; Sigbjørn Lien

Peroxisome proliferator-activated receptor alpha (PPAR alpha) plays a key role in regulating the catabolic pathway of lipids in response to a variety of compounds named peroxisome proliferators (PPs). The cellular responses to PPs differ among mouse/rat and other species and actualize the study in swine, which show close resemblance to human lipid physiology and metabolism. We have isolated the cDNA containing the open reading frame of porcine PPAR alpha whose deduced amino acid sequence revealed an evolutionary distance to mouse/rat that could be implicated in causing the species-dependent response to PPs. Interestingly, an alternatively spliced PPAR alpha mRNA, lacking exon 5, was detected by reverse transcriptase-polymerase chain reaction in several porcine tissues. This deletion alters the reading frame and introduces a premature stop codon of PPAR alpha, presumably giving rise to a C-terminal truncated protein. We have also examined PPAR alpha expression by Northern blot analysis in tissues taken from pigs at three different stages of maturation, including two breeds that differ considerably in body composition and fat deposition. Porcine PPAR alpha was predominantly expressed in kidney and liver in mature individuals. When comparing piglets of a young age, a breed-specific tissue distribution of PPAR alpha mRNA was observed, particularly in liver and heart.


Cell Biology International | 2008

Depot specific differences during adipogenesis of porcine stromal‐vascular cells

Johanna Samulin; Sigbjørn Lien; Eli Grindflek; Ingunn Berget; Bente Ruyter; Hilde Sundvold

Recently a role of adipose tissue as an endocrine organ secreting factors involved in the regulation of whole‐body energy homeostasis has emerged. Preadipocytes in different fat depots have distinct adipogenic potential and the metabolic activity differs between mature adipocytes of different depot origins. Here we describe the proliferation and differentiation of stromal‐vascular cells derived from subcutaneous and visceral fat depots of adult pigs. We demonstrate that subcutaneous porcine preadipocytes proliferate more actively and that individual subcutaneous adipocytes have a more rapid accumulation of triacylglycerols than visceral cells. During differentiation, subcutaneous and visceral preadipocytes showed similar gene expression patterns with increased expression of adiponectin (APM1), adipocyte‐specific fatty acid binding protein (FABP4), catalase (CAT), and peroxisome proliferator‐activated receptor gamma 2 (PPARG2). Furthermore, initial data showing depot‐originated effects on the expression of CAT, carnitine palmitoyl transferase 1B (CPT1B) and FABP4 suggest possible depot specific differences in the function and metabolism of mature porcine adipocytes.


Fish & Shellfish Immunology | 2010

Identification of a novel allele of peroxisome proliferator-activated receptor gamma (PPARG) and its association with resistance to Aeromonas salmonicida in Atlantic salmon (Salmo salar).

Hilde Sundvold; Bente Ruyter; Tone-Kari K Østbye; Thomas Moen

Bacterial and viral diseases are major problems in Atlantic salmon aquaculture, but may be challenged through selection of brood stock with enhanced survival to diseases. Todays selection strategy is based on controlled challenge tests using siblings of the breeding candidates, and is thus indirect. Direct trait records on breeding candidates can potentially be provided through identification of genetic variation linked to the susceptibility to the disease. Peroxisome proliferator-activated receptor gamma (PPARG) is a lipid-sensing transcription factor primarily known for inducing fat-accumulation in adipocytes, but also in lipid-accumulating macrophages, in mammalian species. Here we report a novel allele of PPARG, pparg-2, in Atlantic salmon. pparg-2 has an insertion of sixty nucleotides that encodes two additional copies of the almost perfect decapeptide motif, (F/C/Y)NHSPDR(S/N)HS, compared to the previously described pparg-1. pparg-1 contains six copies of this repeat unit whereas eight copies are present in the novel pparg-2 allele. pparg-2 mRNA was detectable in kidney and spleen of random Atlantic salmon samples. Here, we studied the effect of pparg-1 and pparg-2 on survival upon challenge to a highly virulent bacterium, Aeromonas salmonicida, causing furunculosis, and the virus causing infectious salmon anaemia (ISA), respectively, in a Norwegian aquaculture population of Atlantic salmon. ppar alleles were found to be significantly associated with survival upon challenge to A. salmonicida, but not to ISA. pparg-2 was the better allele in terms of survival in the challenge test for furunculosis, survival rates being 0.32, 0.40 and 0.42 for animals with the pparg-1,-1, pparg-1, -2 and pparg-2, -2 genotypes, respectively. We conclude that pparg-2 is in linkage disequilibrium (LD) with, or identical to, a locus contributing to different susceptibility to furunculosis in Atlantic salmon. PPARG was mapped to linkage group eight (LG8) but could only be positioned on the male linkage map since all the informative parents in the mapping families were males. This is the first report showing an association between pparg alleles and an enhanced immune response in fish.


Animal Genetics | 2009

Expression of DLK1 splice variants during porcine adipocyte development in vitro and in vivo

Johanna Samulin; Paul R. Berg; Hilde Sundvold; Eli Grindflek; Sigbjørn Lien

Delta-like 1 (DLK1) belongs to the epidermal growth factor-like transmembrane protein family and is involved in the regulation of adipogenesis. Several splice variants of DLK1 have been identified in various species, of which two have been previously identified in pig. Here, we present two novel porcine DLK1 splice variants DLK1A and DLK1C. The gene expression profile of these variants together with the previously described DLK1B and DLK1C2 variants was studied in adipose tissue depots of pigs and during adipocyte differentiation in vitro. The short DLK1C and DLK1C2 transcripts were most abundantly expressed and their expression was reduced during porcine adipogenesis.


Comparative Biochemistry and Physiology B | 2009

Changes in lipid metabolism associated gene transcripts during porcine adipogenesis

Johanna Samulin; Ingunn Berget; Eli Grindflek; Sigbjørn Lien; Hilde Sundvold

Pigs are recognised as suitable biomedical models to study obesity and obesity-related diseases; however, little is known about adipose tissue development and adipogenesis in pigs. In this study, the temporal expression of key genes involved in lipid metabolism was investigated during porcine adipogenesis and the metabolic fate of exogenously administered palmitic acid (16:0) was examined in differentiating preadipocytes. The expression of genes encoding elongases and desaturases increased simultaneously with those involved in fatty acid and triacylglycerol synthesis during porcine adipogenesis, and a high biosynthesis of monounsaturated fatty acids was measured prior to storage in differentiating preadipocytes. Although the total fatty acid oxidation in differentiating preadipocytes was low, differentiating cells showed increased expression of hormone-sensitive lipase and mitochondrial and peroxisomal genes. These data provide new insight into the temporal expression of genes involved in lipid metabolism during porcine adipogenesis and suggest a possible role of elongation and desaturation events prior to lipid accumulation in porcine adipocytes.


BMC Genetics | 2011

Characterisation of a novel paralog of scavenger receptor class B member I (SCARB1) in Atlantic salmon (Salmo salar)

Hilde Sundvold; Hanna Helgeland; Matthew Baranski; Stig W. Omholt; Dag Inge Våge

BackgroundRed flesh colour is a unique trait found in some salmonid genera. Carotenoid pigments are not synthesized de novo in the fish, but are provided by dietary uptake. A better understanding of the molecular mechanisms underlying the cellular uptake and deposition of carotenoids could potentially be used to improve the low muscle deposition rate that is typically found in farmed Atlantic salmon. In addition, from an evolutionary point of view, the establishment and maintenance of this trait is still poorly understood. It has been demonstrated in several species that scavenger receptor class B, member 1 (SCARB1) is involved in intestinal absorption of carotenoids, which makes this gene a possible source of genetic variation in salmonid flesh pigmentation.ResultsIn this study, a novel paralog of SCARB1 (SCARB1-2) was detected through screening for genetic variation in Atlantic salmon SCARB1. Full length SCARB1-2 encodes a protein with 89% identity to Atlantic salmon SCARB1, except for the C-terminal cytoplasmic tail that shows only 12% identity. The most prominent site of SCARB1 mRNA expression was in the mid gut, while a five-fold lower level was detected in Atlantic salmon skeletal muscle and liver. The SCARB1-2 mRNA was equally expressed in liver, muscle and mid gut, and at a lower level than SCARB1 mRNA. A total of seven different SCARB1-2 alleles comprising repetitive enhancer of zeste motifs (EZH2) were identified in the founding parents of a resource Atlantic salmon population. We mapped the SCARB1-2 paralog to a region on Atlantic salmon chromosome 1, containing a putative QTL for flesh colour. Addition of the SCARB1-2 marker increased the significance of this QTL, however the large confidence interval surrounding the QTL precludes confirmation of SCARB1-2 as a causative gene underlying variation in this trait.ConclusionWe have characterised a novel paralog of SCARB1 (SCARB1-2), have mapped it to Atlantic salmon chromosome 1 and have described its expression in various tissues. Mapping with SCARB1-2 alleles added further evidence for a QTL affecting flesh colour on this chromosome, however further studies are needed to confirm a functional role for this gene in flesh colour pigmentation.


Gene | 2000

Evolutionary conservation of the apolipoprotein E-C1-C2 gene cluster on bovine chromosome 18q24.

Anna Brzozowska; Hilde Sundvold; Sigbjørn Lien; Sissel Rogne

We have constructed a long-range restriction map spanning about 250 kb on bovine chromosome 18q24. Our results show that the apolipoprotein C2 (APOC2) gene is located about 25 kb from the APOE gene. Four putative CpG islands are also indicated in the map. Interestingly, a minisatellite located in the third intron of the human and mouse APOC2 genes was also found at identical position in the bovine gene and revealed high sequence identity comparing with the two corresponding sequences. By means of cosmid mapping, we further demonstrate that the APOE-APOC1-APOC2 gene cluster is evolutionary conserved in cattle.

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Sigbjørn Lien

Norwegian University of Life Sciences

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Johanna Samulin

Norwegian University of Life Sciences

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Eli Grindflek

Norwegian University of Life Sciences

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Ingunn Berget

Norwegian University of Life Sciences

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Dag Inge Våge

Norwegian University of Life Sciences

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Hanna Helgeland

Norwegian University of Life Sciences

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Stig W. Omholt

Norwegian University of Science and Technology

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