Hiroaki Eguchi

In vitro Comparative Study of the Antitumor Effects of Human Interferon-α, β and γ on the Growth and Invasive Potential of Human Melanoma Cells

We have studied the effects of interferon (IFN)‐α, β, and γ in vitro on the growth and invasive potential of human melanoma SK‐MEL‐118 cells.

A patient with plaque-stage mycosis fungoides has successfully been treated with long-term administration of IFN-γ and has been in complete remission for more than 6 years

Summary We report the successful treatment of a patient with plaque‐stage mycosis fungoides with long‐term and intravenous administration of recombinant human interferon‐γ (IFN‐γ) and discuss the possible mechanisms of this therapy.

Up‐regulation of ICAM‐1 expression on human dermal fibroblasts by IFN β in the presence of TNF‐α

Unstimulated human fibroblasts show low or undetectable ICAM‐1 expression. Interferon‐beta (IFN‐β) at concentrations of 10,100, and 1000 IU/ml in the presence of tumor necrosis factor‐alpha (TNF‐α) significantly increased the ICAM‐I expression of fibroblasts in a dose‐dependent manner. Treatment with IFN‐β alone, however, did not up‐regulate the ICAM‐1 expression. Furthermore the attachment of peripheral blood mononuclear cells (PBMCs) to cytokine‐treated fibroblasts was increased. This augmented attachment was partly inhibited by anti‐ICAM‐1 antibody. These results suggest that IFN‐β and TNF‐α may cooperatively modulate the attachment of PBMCs in the dermis.