Hirofumi Miyata

Fibre conduction velocity and fibre composition in human vastus lateralis.

SummaryThe relationship between muscle fibre composition and fibre conduction velocity was investigated in 19 male track athletes, 12 sprinters and 7 distance runners, aged 20–24 years, using needle biopsy samples from vastus lateralis. Cross sectional areas of the fast twitch (FT) and slow twitch (ST) fibres were determined by histochemical analysis. The percentage of FT fibre areas ranged from 22.6 to 93.6%. Sprinters had a higher percentage of FT fibres than distance runners. Muscle fibre conduction velocity was measured with a surface electrode array placed along the muscle fibres, and calculated from the time delay between 2 myoelectric signals recorded during a maximal voluntary contraction. The conduction velocity ranged from 4.13 to 5.20 m·s−1. A linear correlation between conduction velocity and the relative area of FT fibres was statistically significant (r = 0.84,p<0.01). This correlation indicates that muscle fibre composition can be estimated from muscle fibre conduction velocity measured noninvasively with surface electrodes.

Inactivity-induced remodeling of neuromuscular junctions in rat diaphragmatic muscle.

We hypothesized that inactivity‐induced remodeling of neuromuscular junctions (NMJs) depends on fiber type and the match between muscle fiber and motoneuron (MN) activities. Two inactivity models were studied in rat diaphragmatic muscle: spinal hemisection at C2 (SH), where both diaphragmatic muscle fibers and phrenic MNs were inactive, and tetrodotoxin (TTX) nerve blockade, where only muscle fibers were inactive. After 2 weeks of inactivity, there was increased number of pre‐ and postsynaptic branches (fragmentation) of NMJs at type IIx/b fibers in both models. In addition, planar NMJ areas at type IIx/b fibers in the SH model were enlarged. In contrast, NMJs at type I and IIa fibers were unaffected in both SH and TTX models. Functionally, neuromuscular transmission in diaphragmatic muscle fibers improved in the SH model, but worsened in the TTX model, compared to controls. These results suggest that NMJ remodeling depends on the level of MN activity. The relative preservation of NMJs at type I and IIa fibers suggests a potential for recovery from diaphragmatic paralysis in the clinical setting, at least for respiratory behaviors.

Morphological changes in the masseter muscle and its motoneurons during postnatal development.

It has been suggested that the morphological properties of the masseter muscle are changed by the masticatory activity pattern. In the rat, the activity pattern of the muscle alters from sucking to biting around 3 weeks after birth. The working hypothesis in this study is that the unique alteration in masticatory activity has an important influence on the development of the masseter muscle and its motoneurons.

Muscle fiber population and biochemical properties of whole body muscles in thoroughbred horses.

We examine the muscle fiber population and metabolic properties of skeletal muscles from the whole body in Thoroughbred horses. Postmortem samples were taken from 46 sites in six Thoroughbred horses aged between 3 and 6 years. Fiber type population was determined on muscle fibers stained with monoclonal antibody to each myosin heavy chain isoform and metabolic enzyme activities were determined spectrophotometrically. Histochemical analysis demonstrated that most of the muscles had a high percentage of Type IIa fibers. In terms of the muscle characteristic in several parts of the horse body, the forelimb muscles had a higher percentage of Type IIa fiber and a significantly lower percentage of Type IIx fiber than the hindlimb muscles. The muscle fiber type populations in the thoracic and trunk portion were similar to those in the hindlimb portion. Biochemical analysis indicated high succinate dehydrogenase activity in respiratory‐related muscle and high phosphofructokinase activity in hindlimbs. We suggested that the higher percentage of Type IIa fibers in Thoroughbred racehorses is attributed to training effects. To consider further the physiological significance of each part of the body, data for the recruitment pattern of each muscle fiber type during exercise are needed. The muscle fiber properties in this study combined with the recruitment data would provide fundamental information for physiological and pathological studies in Thoroughbred horses. Anat Rec, 2009.

Effect of controlled exercise on middle gluteal muscle fibre composition in Thoroughbred foals

REASONS FOR PERFORMING STUDY Most racehorses are trained regularly from about age 18 months; therefore, little information is available on the effect of training in Thoroughbred foals. HYPOTHESIS Well-controlled exercise could improve muscle potential ability for endurance running. METHODS Thoroughbred foals at age 2 months were separated into control and training (treadmill exercise) groups and samples obtained from the middle gluteal muscle at 2 and 12 months post partum. Muscle fibre compositions were determined by histochemical and electrophoretical techniques and succinic dehydrogenase (SDH) activity was analysed in each fibre type. RESULTS All fibre types were hypertrophied with growth and type I and IIA fibres were significantly larger in the training than the control group at age 12 months. A significant increase of SDH activity was found in type IIX muscle fibres in the training group. CONCLUSIONS Training in young Thoroughbred horses can facilitate muscle fibre hypertrophy and increase the oxidative capacity of type IIX fibres, which could potentially enhance stamina at high speeds. POTENTIAL RELEVANCE To apply this result to practical training, further studies are needed to determine more effective and safe intensities of controlled exercise.

Muscle satellite cells are activated after exercise to exhaustion in Thoroughbred horses.

REASONS FOR PERFORMING STUDY Although satellite cells are well known as muscle stem cells capable of adding myonuclei during muscle repair and hypertrophy, the response of satellite cells in horse muscles to a run to exhaustion is still unknown. OBJECTIVES To investigate the time course of satellite cell activation in Thoroughbred horse muscle after running to exhaustion. We hypothesised that this type of intense exercise would induce satellite cell activation in skeletal muscle similar to a resistance exercise. METHODS Nine de-trained Thoroughbred horses (6 geldings and 3 mares) aged 3-6 years were studied. Biopsy samples were taken from the gluteus medius muscle of the horses before and 1 min, 3 h, 1 day, 3 days, 1 week and 2 weeks after a treadmill run to exhaustion. The numbers of satellite cells for each fibre type were determined by using immunofluorescence staining. Total RNA was extracted from these samples, and the expressions of interleukin (IL)-6, paired box transcriptional factor (Pax) 7, myogenic differentiation 1 (MyoD), myogenin, proliferating cell nuclear antigen (PCNA), insulin-like growth factor (IGF)-I and hepatocyte growth factor (HGF) mRNA were analysed using real-time reverse transcription-PCR. RESULTS The numbers of satellite cells were significantly increased in type I and IIa fibres at 1 week and in type IIa/x fibre at 2 weeks post exercise. The expression of IL-6 mRNA increased significantly by 3 h post exercise. The expression of PCNA mRNA also increased by 1 day after running, indicating that running can initiate satellite cell proliferation. The expression of Pax7, MyoD, myogenin, IGF-I and HGF mRNA peaked at 1 week post exercise. CONCLUSION Satellite cell activation and proliferation could be enhanced after a run to exhaustion without detectable injury as assessed by the histochemical analysis. Understanding the response of satellite cell activation to running exercise provides fundamental information about the skeletal muscle adaptation in Thoroughbred horses.

Relationship between soma diameter and oxidative enzyme activity of α-motoneurons

Abstract In order to determine whether there is a relationship between soma diameter and oxidative enzyme activity of α-motoneurons, we investigated the α-motoneurons innervating the different portions within a rat gluteus medius muscle. Two fluorescent neuronal tracers, Nuclear yellow and Fast blue, were used for labeling motoneurons innervating the deep (predominance of oxidative fibers) and superficial (predominance of non-oxidative fibers) portions of the muscle. An inverse relationship between soma diameter and oxidative enzyme activity was not seen in the motoneuron pool innervating either the deep or superficial portions. When the two portions were taken together, however, the inverse relationship was seen. The inverse relationship seemed to be demonstrated in a motoneuron pool which intermingled motoneurons innervating oxidative fibers and motoneurons innervating non-oxidative fibers. These results suggest that the oxidative enzyme activity of α-motoneurons is not correlated strictly with their soma size. We consider that the oxidative enzyme activity of α-motoneurons is correlated with oxidative enzyme activity of the muscle unit.

Soma diameter and oxidative enzyme activity of identified α-motoneurons: application of a retrograde fluorescent neuronal tracer

Using a fluorescent compound as a retrograde neuronal tracer for the identification of a specific motoneuron pool, we examined the soma diameter and oxidative enzyme activity of alpha-motoneurons innervating the extensor digitorum longus (EDL) and the soleus (Sol) muscles in the rat. An inverse relationship between soma diameter and oxidative enzyme activity was demonstrated in the EDL, while there was no significant relationship between these variables in the Sol. These results suggest that an inverse relationship between soma diameter and oxidative enzyme activity cannot always be demonstrated in all of the motoneuron pools.

Investigation and characterization of rat bipedal walking models established by a training program

The purpose of the present study was to establish a rat bipedal walking model to examine the effects of bipedal walking on the central nervous system by training rats to perform bipedal walking over a period of 3 months. The characteristics of bipedal walking were investigated using kinematic and electromyographic methods in established bipedal walking models. Stable bipedal walking was achieved in rats by training them to stand with an upright posture and to walk with the hindlimbs using bipedal-walking training equipment to obtain a water reward. A stable head position in the rat bipedal walking model was attained primarily by closing the swing-phase period with a large angular change in the hip, knee, and ankle joints. The EMG burst pattern of the knee extensor (m. rectus femoris) and the erector muscle of the spine (m. longissimus) during bipedal walking was similar to that during quadrupedal walking in rats. We established two bipedal walking models using normal and forelimb-amputated rats. Comparative studies of these two bipedal walking models are expected to provide the information about the influence of forelimb movements on neuronal control of bipedal walking.

Inactivity Alters Structural and Functional Properties of the Neuromuscular Junction

The diaphragm (DIAm) is the most important inspiratory muscle involved in mammalian ventilation, and accordingly it has a high duty cycle (fraction of time active versus inactive; ∼40% for the rat DIAm (7, 8)), compared to limb muscles (∼2% for the soleus muscle to ∼15% for the extensor digitorum longus muscle (5)). This unique activation pattern of the DIAm might make it particularly susceptible to inactivity.